LOCUS       NM_003345               2850 bp    mRNA    linear   PRI 17-MAR-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 I (UBE2I), transcript
            variant 1, mRNA.
ACCESSION   NM_003345
VERSION     NM_003345.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Wang,Y., Zou,H., Ji,W., Huang,M., You,B., Sun,N., Qiao,Y., Liu,P.,
            Xu,L., Zhang,X., Cai,M., Kuang,Y., Fu,S., Sun,W., Jia,X. and Wu,J.
  TITLE     Repression of the SUMO-conjugating enzyme UBC9 is associated with
            lowered double minutes and reduced tumor progression
  JOURNAL   Cancer Biol Ther 25 (1), 2323768 (2024)
   PUBMED   38465861
  REMARK    GeneRIF: Repression of the SUMO-conjugating enzyme UBC9 is
            associated with lowered double minutes and reduced tumor
            progression.
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Zhu,Q., Liang,P., Meng,H., Li,F., Miao,W., Chu,C., Wang,W., Li,D.,
            Chen,C., Shi,Y., Yu,X., Ping,Y., Niu,C., Wu,H.B., Zhang,A.,
            Bian,X.W. and Zhou,W.
  TITLE     Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and
            protein sumoylation in glioma stem cells
  JOURNAL   Nat Commun 15 (1), 40 (2024)
   PUBMED   38167292
  REMARK    GeneRIF: Stabilization of Pin1 by USP34 promotes Ubc9 isomerization
            and protein sumoylation in glioma stem cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2850)
  AUTHORS   Gu,Y., Feng,X., Jin,Y., Liu,Y., Zeng,L., Zhou,D. and Feng,Y.
  TITLE     Upregulation of miRNA-10a-5p promotes tumor progression in cervical
            cancer by suppressing UBE2I signaling
  JOURNAL   J Obstet Gynaecol 43 (1), 2171283 (2023)
   PUBMED   36744815
  REMARK    GeneRIF: Upregulation of miRNA-10a-5p promotes tumor progression in
            cervical cancer by suppressing UBE2I signaling.
REFERENCE   4  (bases 1 to 2850)
  AUTHORS   Huang,C., Yin,Y., Pan,P., Huang,Y., Chen,S., Chen,J., Wang,J.,
            Xu,G., Tao,X., Xiao,X., Li,J., Yang,J., Jin,Z., Li,B., Tong,Z.,
            Du,W., Liu,L. and Liu,Z.
  TITLE     The Interaction between SARS-CoV-2 Nucleocapsid Protein and UBC9
            Inhibits MAVS Ubiquitination by Enhancing Its SUMOylation
  JOURNAL   Viruses 15 (12), 2304 (2023)
   PUBMED   38140545
  REMARK    GeneRIF: The Interaction between SARS-CoV-2 Nucleocapsid Protein
            and UBC9 Inhibits MAVS Ubiquitination by Enhancing Its SUMOylation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2850)
  AUTHORS   Huang,J., Tan,X., Liu,Y., Jiang,K. and Luo,J.
  TITLE     Knockdown of UBE2I inhibits tumorigenesis and enhances
            chemosensitivity of cholangiocarcinoma via modulating p27kip1
            nuclear export
  JOURNAL   Mol Carcinog 62 (5), 700-715 (2023)
   PUBMED   36825757
  REMARK    GeneRIF: Knockdown of UBE2I inhibits tumorigenesis and enhances
            chemosensitivity of cholangiocarcinoma via modulating p27kip1
            nuclear export.
REFERENCE   6  (bases 1 to 2850)
  AUTHORS   Yasugi,T. and Howley,P.M.
  TITLE     Identification of the structural and functional human homolog of
            the yeast ubiquitin conjugating enzyme UBC9
  JOURNAL   Nucleic Acids Res 24 (11), 2005-2010 (1996)
   PUBMED   8668529
REFERENCE   7  (bases 1 to 2850)
  AUTHORS   Jiang,W. and Koltin,Y.
  TITLE     Two-hybrid interaction of a human UBC9 homolog with centromere
            proteins of Saccharomyces cerevisiae
  JOURNAL   Mol Gen Genet 251 (2), 153-160 (1996)
   PUBMED   8668125
REFERENCE   8  (bases 1 to 2850)
  AUTHORS   Kovalenko,O.V., Plug,A.W., Haaf,T., Gonda,D.K., Ashley,T.,
            Ward,D.C., Radding,C.M. and Golub,E.I.
  TITLE     Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51
            recombination protein and localizes in synaptonemal complexes
  JOURNAL   Proc Natl Acad Sci U S A 93 (7), 2958-2963 (1996)
   PUBMED   8610150
REFERENCE   9  (bases 1 to 2850)
  AUTHORS   Gottlicher,M., Heck,S., Doucas,V., Wade,E., Kullmann,M., Cato,A.C.,
            Evans,R.M. and Herrlich,P.
  TITLE     Interaction of the Ubc9 human homologue with c-Jun and with the
            glucocorticoid receptor
  JOURNAL   Steroids 61 (4), 257-262 (1996)
   PUBMED   8733011
REFERENCE   10 (bases 1 to 2850)
  AUTHORS   Watanabe,T.K., Fujiwara,T., Kawai,A., Shimizu,F., Takami,S.,
            Hirano,H., Okuno,S., Ozaki,K., Takeda,S., Shimada,Y., Nagata,M.,
            Takaichi,A., Takahashi,E., Nakamura,Y. and Shin,S.
  TITLE     Cloning, expression, and mapping of UBE2I, a novel gene encoding a
            human homologue of yeast ubiquitin-conjugating enzymes which are
            critical for regulating the cell cycle
  JOURNAL   Cytogenet Cell Genet 72 (1), 86-89 (1996)
   PUBMED   8565643
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK291118.1, BC000427.2,
            AL031714.8 and BI793063.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003345.4.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes a member of the E2
            ubiquitin-conjugating enzyme family. Four alternatively spliced
            transcript variants encoding the same protein have been found for
            this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript. Variants 1-4 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.432179.1,
                                           SRR3476690.531737.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397514.8/ ENSP00000380649.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-286               AK291118.1         1-286
            287-1094            BC000427.2         200-1007
            1095-2832           AL031714.8         13966-15703
            2833-2850           BI793063.1         102-119
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..2850
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /note="ubiquitin conjugating enzyme E2 I"
                     /db_xref="GeneID:7329"
                     /db_xref="HGNC:HGNC:12485"
                     /db_xref="MIM:601661"
     exon            1..138
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /inference="alignment:Splign:2.1.0"
     exon            139..215
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..626
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /EC_number="6.3.2.19"
                     /note="ubiquitin-conjugating enzyme E2I (homologous to
                     yeast UBC9); ubiquitin-conjugating enzyme E2I (UBC9
                     homolog, yeast); SUMO-1-protein ligase; ubiquitin-like
                     protein SUMO-1 conjugating enzyme; ubiquitin-conjugating
                     enzyme UbcE2A; ubiquitin conjugating enzyme E2I;
                     ubiquitin-protein ligase E2I; ubiquitin conjugating enzyme
                     9; SUMO-protein ligase; ubiquitin-protein ligase I;
                     ubiquitin carrier protein 9; ubiquitin carrier protein I;
                     RING-type E3 SUMO transferase UBC9"
                     /codon_start=1
                     /product="SUMO-conjugating enzyme UBC9"
                     /protein_id="NP_003336.1"
                     /db_xref="CCDS:CCDS10433.1"
                     /db_xref="GeneID:7329"
                     /db_xref="HGNC:HGNC:12485"
                     /db_xref="MIM:601661"
                     /translation="MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECA
                     IPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKD
                     WRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS"
     exon            216..299
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /inference="alignment:Splign:2.1.0"
     exon            300..372
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /inference="alignment:Splign:2.1.0"
     exon            373..482
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /inference="alignment:Splign:2.1.0"
     exon            483..562
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /inference="alignment:Splign:2.1.0"
     exon            563..2850
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1165..1170
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /note="hexamer: AATAAA"
     polyA_site      1183
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /note="major polyA site"
     regulatory      2832..2837
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
                     /note="hexamer: ATTAAA"
     polyA_site      2850
                     /gene="UBE2I"
                     /gene_synonym="C358B7.1; P18; UBC9"
ORIGIN      
        1 agggtcctcg gagctgctct ggctgcgcgc ggagcgggct ccggagggaa gtcccgagac
       61 aaagggaagc gccgccgccg ccgccccgct cggtcctcca cctgtccgct acgctcgccg
      121 gggctgcggc cgcccgaggg actttgaaca tgtcggggat cgccctcagc agactcgccc
      181 aggagaggaa agcatggagg aaagaccacc catttggttt cgtggctgtc ccaacaaaaa
      241 atcccgatgg cacgatgaac ctcatgaact gggagtgcgc cattccagga aagaaaggga
      301 ctccgtggga aggaggcttg tttaaactac ggatgctttt caaagatgat tatccatctt
      361 cgccaccaaa atgtaaattc gaaccaccat tatttcaccc gaatgtgtac ccttcgggga
      421 cagtgtgcct gtccatctta gaggaggaca aggactggag gccagccatc acaatcaaac
      481 agatcctatt aggaatacag gaacttctaa atgaaccaaa tatccaagac ccagctcaag
      541 cagaggccta cacgatttac tgccaaaaca gagtggagta cgagaaaagg gtccgagcac
      601 aagccaagaa gtttgcgccc tcataagcag cgaccttgtg gcatcgtcaa aaggaaggga
      661 ttggtttggc aagaacttgt ttacaacatt tttgcaaatc taaagttgct ccatacaatg
      721 actagtcacc tgggggggtt gggcgggcgc catcttccat tgccgccgcg ggtgtgcggt
      781 ctcgattcgc tgaattgccc gtttccatac agggtctctt ccttcggtct tttgtatttt
      841 tgattgttat gtaaaactcg cttttatttt aatattgatg tcagtatttc aactgctgta
      901 aaattataaa cttttatact tgggtaagtc ccccaggggc gagttcctcg ctctgggatg
      961 caggcatgct tctcaccgtg cagagctgca cttggcctca gctggctgta tggaaatgca
     1021 ccctccctcc tgccgctcct ctctagaacc ttctagaacc tgggctgtgc tgcttttgag
     1081 cctcagaccc caggtcagca tctcggttct gcgccacttc ctttgtgttt atatggcgtt
     1141 ttgtctgtgt tgctgtttag agtaaataaa ctgtttatat aaaggttttg gttgcattat
     1201 tatcattgaa agtgagagga ggcggcctcc cagtgcccgg ccctccccac ccacctgcag
     1261 ccccaccgcg ggccaggacc aggctctcca tctgcttcgg atgcacgcag gctgtgaggc
     1321 tctgtcttgc cctggatctt tgtaaacagg gctgtgtaca aagtgctgct gaggtttctg
     1381 tgctccccgc atctgcgggc tgtagagcgc tgggcagcta agatctgcat aggtcgggat
     1441 tggcatcgag accctggcaa ctgcaccggt gccagctgtc ttgggggcca caaggccagg
     1501 tccagaccag ggctgggggc tgcctgagga ctcctatccg ggcagcctgc tggcgggggt
     1561 tcccctcttc agtggccagg tcacagggat ggagctgcgc tgtgcatagg gtgccacctc
     1621 aggtgtctgt cccttgtgtc ctcaggaggc agccttgcta ccacccgtgg caaacgccag
     1681 gtgctttttc tgggagagcc cacagccgtg gccctccagg gcttccccga cccttagcgc
     1741 caggtagagg gccctgggca gcctgtgtct ggaattcttc gtcctgaggc cacctgagtg
     1801 tggtctgtcc tggggaggct gtgcgcctca gcagccgtcc tgacgctgag ccctctgcaa
     1861 aggttgggcc ggccaggcct cttggggctg cctgagccac tgcaggaagt ggcctggctg
     1921 ggaagttggg tgccggtcac ctcccagcag gaaggcacag tggacagaga tgggaagccc
     1981 tgggggacac agcccggtgc tcccagccct ccaacctctg gctcccaacc cagtctcccc
     2041 atcctagcga gcttggccct cctcagtttc gtttcaagcc ttggggctgg agctggccct
     2101 gctgccctgg caccccccgg tggctggagc tgggtccccg tggcccaagt gcagggtccc
     2161 aagagggcag ggcggggctc cccaaaggag caaagaatgc agggagggcg gtccagggcc
     2221 ctgggaaggg gagctcggca ccctccaggt ccgtgtggga ctccagccgc tgttggctgg
     2281 gaatcgaagt tagaggtgac ttccaaaggc cccccgagcc ggcagtgccc cccaccaccc
     2341 ctccagcgac tctgcggtgc cagtgccttg ttggcttttc cggctacgca ccctgcagtc
     2401 actgagctct cggtctgacg tctgatgttt gtggtttgtt tataacacgg ggccttacct
     2461 ggggaattca gctggtttga atatttgtag cccgctccca gaatgtctta ttttgtaatg
     2521 actgaactac atttagtaat agttacacat gtatatggtt aatacatatg gaaattcaat
     2581 atattttgta gttaacgtat tctgaagtaa cggatgtttc tcgccaatcg tagtgacttc
     2641 agctaacgaa atgttctttt gtagtaccac ggtcctcggc ctaacgaagg acgtgaacct
     2701 tgtaagagga gagctctgaa acgcggtcac ctttgtttag tggaagggaa agtgtgttcc
     2761 cggcatgagg tgcctcggaa ttagtaaaga attgtgggca atggattaac cactgtatct
     2821 aagaatccac cattaaagca tttgcacaga
//
