LOCUS       NM_003343               3367 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 G2 (UBE2G2),
            transcript variant 1, mRNA.
ACCESSION   NM_003343
VERSION     NM_003343.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Cremer,T., Hoelen,H., van de Weijer,M.L., Janssen,G.M., Costa,A.I.,
            van Veelen,P.A., Lebbink,R.J. and Wiertz,E.J.H.J.
  TITLE     Proinsulin degradation and presentation of a proinsulin B-chain
            autoantigen involves ER-associated protein degradation
            (ERAD)-enzyme UBE2G2
  JOURNAL   PLoS One 19 (5), e0287877 (2024)
   PUBMED   38787820
  REMARK    GeneRIF: Proinsulin degradation and presentation of a proinsulin
            B-chain autoantigen involves ER-associated protein degradation
            (ERAD)-enzyme UBE2G2.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Smith,C.E., Tsai,Y.C., Liang,Y.H., Khago,D., Mariano,J., Li,J.,
            Tarasov,S.G., Gergel,E., Tsai,B., Villaneuva,M., Clapp,M.E.,
            Magidson,V., Chari,R., Byrd,R.A., Ji,X. and Weissman,A.M.
  TITLE     A structurally conserved site in AUP1 binds the E2 enzyme UBE2G2
            and is essential for ER-associated degradation
  JOURNAL   PLoS Biol 19 (12), e3001474 (2021)
   PUBMED   34879065
  REMARK    GeneRIF: A structurally conserved site in AUP1 binds the E2 enzyme
            UBE2G2 and is essential for ER-associated degradation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3367)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 3367)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3367)
  AUTHORS   van de Weijer,M.L., Schuren,A.B.C., van den Boomen,D.J.H.,
            Mulder,A., Claas,F.H.J., Lehner,P.J., Lebbink,R.J. and
            Wiertz,E.J.H.J.
  TITLE     Multiple E2 ubiquitin-conjugating enzymes regulate human
            cytomegalovirus US2-mediated immunoreceptor downregulation
  JOURNAL   J Cell Sci 130 (17), 2883-2892 (2017)
   PUBMED   28743740
  REMARK    GeneRIF: UBE2G2 was crucial for the degradation of various
            immunoreceptors. UBE2J2, on the other hand, counteracted
            US2-induced endoplasmic reticulum-associated protein degradation by
            downregulating TRC8 expression.
REFERENCE   6  (bases 1 to 3367)
  AUTHORS   Huang,L., Kinnucan,E., Wang,G., Beaudenon,S., Howley,P.M.,
            Huibregtse,J.M. and Pavletich,N.P.
  TITLE     Structure of an E6AP-UbcH7 complex: insights into ubiquitination by
            the E2-E3 enzyme cascade
  JOURNAL   Science 286 (5443), 1321-1326 (1999)
   PUBMED   10558980
REFERENCE   7  (bases 1 to 3367)
  AUTHORS   Moynihan,T.P., Ardley,H.C., Nuber,U., Rose,S.A., Jones,P.F.,
            Markham,A.F., Scheffner,M. and Robinson,P.A.
  TITLE     The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with
            RING finger/IBR motif-containing domains of HHARI and H7-AP1
  JOURNAL   J Biol Chem 274 (43), 30963-30968 (1999)
   PUBMED   10521492
REFERENCE   8  (bases 1 to 3367)
  AUTHORS   Katsanis,N. and Fisher,E.M.
  TITLE     Identification, expression, and chromosomal localization of
            ubiquitin conjugating enzyme 7 (UBE2G2), a human homologue of the
            Saccharomyces cerevisiae ubc7 gene
  JOURNAL   Genomics 51 (1), 128-131 (1998)
   PUBMED   9693041
REFERENCE   9  (bases 1 to 3367)
  AUTHORS   Rose,S.A., Leek,J.P., Moynihan,T.P., Ardley,H.C., Markham,A.F. and
            Robinson,P.A.
  TITLE     Assignment1 of the ubiquitin conjugating enzyme gene, UBE2G2, to
            human chromosome band 21q22.3 by in situ hybridization
  JOURNAL   Cytogenet Cell Genet 83 (1-2), 98-99 (1998)
   PUBMED   9925943
REFERENCE   10 (bases 1 to 3367)
  AUTHORS   Chen,P., Johnson,P., Sommer,T., Jentsch,S. and Hochstrasser,M.
  TITLE     Multiple ubiquitin-conjugating enzymes participate in the in vivo
            degradation of the yeast MAT alpha 2 repressor
  JOURNAL   Cell 74 (2), 357-369 (1993)
   PUBMED   8393731
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK290629.1, BC008351.1,
            AL773604.1 and BU741369.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003343.5.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes a member of the E2
            ubiquitin-conjugating enzyme family. The encoded protein shares
            100% sequence identity with the mouse counterpart. This gene is
            ubiquitously expressed, with high expression seen in adult muscle.
            Three alternatively spliced transcript variants encoding distinct
            isoforms have been found for this gene. [provided by RefSeq, Jan
            2011].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.41495.1,
                                           SRR1660805.240636.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000345496.7/ ENSP00000338348.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AK290629.1         17-40
            25-2568             BC008351.1         1-2544
            2569-3348           AL773604.1         88903-89682         c
            3349-3367           BU741369.1         18-36               c
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..3367
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
                     /note="ubiquitin conjugating enzyme E2 G2"
                     /db_xref="GeneID:7327"
                     /db_xref="HGNC:HGNC:12483"
                     /db_xref="MIM:603124"
     exon            1..115
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..570
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
                     /EC_number="2.3.2.23"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin-conjugating enzyme E2G 2 (homologous to yeast
                     UBC7); ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog,
                     yeast); ubiquitin conjugating enzyme G2; ubiquitin carrier
                     protein G2; ubiquitin-protein ligase G2; ubiquitin
                     conjugating enzyme 7; E2 ubiquitin-conjugating enzyme G2"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 G2 isoform 1"
                     /protein_id="NP_003334.2"
                     /db_xref="CCDS:CCDS13714.1"
                     /db_xref="GeneID:7327"
                     /db_xref="HGNC:HGNC:12483"
                     /db_xref="MIM:603124"
                     /translation="MAGTALKRLMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGP
                     EDTCFEFGVFPAILSFPLDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMG
                     YESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQ
                     KSLGL"
     exon            116..151
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
                     /inference="alignment:Splign:2.1.0"
     exon            152..197
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
                     /inference="alignment:Splign:2.1.0"
     exon            198..316
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
                     /inference="alignment:Splign:2.1.0"
     exon            317..457
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
                     /inference="alignment:Splign:2.1.0"
     exon            458..3367
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2878..2883
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
                     /note="hexamer: AATAAA"
     polyA_site      2907
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
                     /note="major polyA site"
     regulatory      3339..3344
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
                     /note="hexamer: AATAAA"
     polyA_site      3367
                     /gene="UBE2G2"
                     /gene_synonym="UBC7"
ORIGIN      
        1 agtccccggt gtcggggcag gaggcacgcg cgcggctgag gcgaggtcgc tcggcgcagc
       61 tgttgcgggg ccatggcggg gaccgcgctc aagaggctga tggccgagta caaacaatta
      121 acactgaatc ctccggaagg aattgtagca ggccccatga atgaagagaa cttttttgaa
      181 tgggaggcat tgatcatggg cccagaagac acctgctttg agtttggtgt ttttcctgcc
      241 atcctgagtt tcccacttga ttacccgtta agtcccccaa agatgagatt tacctgtgag
      301 atgtttcatc ccaacatcta ccctgatggg agagtctgca tttccatcct ccacgcgcca
      361 ggcgatgacc ccatgggcta cgagagcagc gcggagcggt ggagtcctgt gcagagtgtg
      421 gagaagatcc tgctgtcggt ggtgagcatg ctggcagagc ccaatgacga aagtggagct
      481 aacgtggatg cgtccaaaat gtggcgcgat gaccgggagc agttctataa gattgccaag
      541 cagatcgtcc agaagtctct gggactgtga gacctggcct cgcacaggcg cgcacacacc
      601 gccaagcagc tcagcattct cccccggcac acttagtgac agtgatgctc tgtgctggta
      661 ccaaacaagg cagacttgca agaaccatgg catctttttt ttttttcaaa cctttcctac
      721 ttcaaacagg cttctcttct gaaatgatga cttaatgtcg aatattgaca gcttactgca
      781 gttttacagt attcctcaca aagggcttca ggtagattat cagagctgtc agcactacct
      841 ctccccgctg aaaccagcag ttcatggctt cctgtggatt ccctccctcc ctggagtgtt
      901 gagggggttg tacctgccag acttccaggg gacgatggaa tacccagaac gctccttctg
      961 aagaaatggg gccctgtagc tgcagcacag gggaagggcc cggcaccctt tctgggtcct
     1021 tcctggttcc ctgtgggccc catgaggagt ccattacttc ctttcttcct tcatatttta
     1081 caggcagatg cttttcttat aatctaatta catcttttca tttgttatat attacaaacc
     1141 atcacactta gaaatacttc caggaaatgc ttttttgaag tgtgaattaa taagaaatgg
     1201 ggtaaataga aaagaaattt attgctgatt ggccaggtgc ggtggttcgt gcctgtaatc
     1261 ccagctcttt gggaggccaa ggcaggtaga tcacaaggtc aggaaattga gaccatcctg
     1321 gctaatacag tgaaacccca tgtctgctaa aattacaaaa aattagctgg gcgtggtggt
     1381 gcacgcctgt agtctcagct actcaggagg ctgaggcagg agaatcgctt gaacccggga
     1441 ggcagaggta gcagtgagct gaagtcccgc cactgcactc cagcctgggc aacagagcga
     1501 gactcagtct caaaaagaaa aaagaaattt attgctgatc acaaggacag acagtttttt
     1561 cccgaccata ctcatcaaag atttacgttt gtatattagt aactagtgca ttactagagc
     1621 aggtgcaggt gaggtcttta aagtttcaat gaaagtttct tctggatcta cagaaaaaat
     1681 tttttttttt caatctaaaa actggaaatt ctagggtttt tgtacatttt ggatgcactg
     1741 ggaatttatt agcacaaaat cattctttgc aactcaaaat tcagaaggga ctctaccata
     1801 tcttagctca gagcacagag gagtgcctta tccccacact tgactgggct gtggaggtgg
     1861 gcatgtgggc ccctgggccc aggctgggga cagagccctt gttttgtgac ttaggatttt
     1921 gatgtggttc ccatgttctc taacagggcc agctgagcag cacaggccag gaggccacag
     1981 tgtaagcaat aacagatctg ccacatgcag aagcaaatat caggcctgtc gcacacgggc
     2041 ggcatttaaa taggaatttc tatttttgaa ataagggatg gtctatgagg catacagtag
     2101 atttgatgtg atccttttct ccctcccttc cataatggat cgtggtctgt gtgactgaac
     2161 ccacacagag tgtcatgggt gacagtttct ggttgaagta gctccacgcc tggcttctgt
     2221 ggacagcaga ttcttttcct tctcacaagg ggctcattta aaatttggag gctgggtgct
     2281 gtggctcacg cctgcaatcc cagcactttg ggagactgag gcgggcggat catgaggtca
     2341 ggagatcgcg accatcctgg ctaacagtga aaccctgtct ccactaaaaa tacaaaaaat
     2401 tagccgggcg tggtggcggg cgcctgtagt cccagctact ctgaagactg aggcaggaga
     2461 atggcgtgaa cccaggaggc ggagcttgca gtgagctgag atcacgccac tgcactccag
     2521 cctgggcaac agagtgagac tctgtctcaa aaaaaaaaaa aaaaaaaatg gaacgcaggg
     2581 caagaactcg tatttggaag gagatggggg aaaggagcgg tattatacct atgttgtatt
     2641 tgcaggcaaa tgagatggag ccctctctgt aaagaagagt catttgtgca agtagacggg
     2701 gtctgtgggt gcaggccctg gaggggcaca caattgcctg gaggcttctg tgagatcggg
     2761 agagggagga gaggcagtct cttgacaaaa taaagtattt ttattcattt gtatttatta
     2821 aatgaaaaaa caatcccatg gtgtccctgt tgtgtggtgg aacctaatga ctgttgaaat
     2881 aaagttctgt gttttccctg ccctgcagtg tgtgtccctt tgtcgtattg tcagaggagc
     2941 ctgtggccac tgcccccagg gagcacacca gatcatccca gctgggattc ctcacggtgg
     3001 gggcttttct gcagacttgg gttagctctg gcatgttttc ttctgtttct gatctccctt
     3061 ctgtcattct gtggtcttcc tctggaattc tcaggttagt tggggtctgt gagacggatg
     3121 tgttcactaa cctataaatg acattttgca gctttttcag cagatgccat tgtgtgagtg
     3181 gcacatttga ccactagaag ccagcaggct gttccgactg cgcttccctc ctgcagttgc
     3241 ttagaagcac agctccctgt catcactcct ctgtggtcct ctgtggtcac ggttggttgc
     3301 gatgagacct gcctctgcgt cttgttactt agcatgtcaa taaagaggca gattcctgtg
     3361 tcagtga
//
