LOCUS       NM_003342               4167 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 G1 (UBE2G1), mRNA.
ACCESSION   NM_003342
VERSION     NM_003342.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Hill,S., Reichermeier,K., Scott,D.C., Samentar,L.,
            Coulombe-Huntington,J., Izzi,L., Tang,X., Ibarra,R., Bertomeu,T.,
            Moradian,A., Sweredoski,M.J., Caberoy,N., Schulman,B.A.,
            Sicheri,F., Tyers,M. and Kleiger,G.
  TITLE     Robust cullin-RING ligase function is established by a multiplicity
            of poly-ubiquitylation pathways
  JOURNAL   Elife 8, e51163 (2019)
   PUBMED   31868589
  REMARK    GeneRIF: UBE2R2 alone had negligible ubiquitylation activity at
            physiological concentrations and the ablation of UBE2R1/2 had no
            effect on the stability of SCF substrates in cells. A genome-wide
            CRISPR screen revealed that an additional E2 enzyme, UBE2G1,
            buffers against the loss of UBE2R1/2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4167)
  AUTHORS   Lu,G., Weng,S., Matyskiela,M., Zheng,X., Fang,W., Wood,S.,
            Surka,C., Mizukoshi,R., Lu,C.C., Mendy,D., Jang,I.S., Wang,K.,
            Marella,M., Couto,S., Cathers,B., Carmichael,J., Chamberlain,P. and
            Rolfe,M.
  TITLE     UBE2G1 governs the destruction of cereblon neomorphic substrates
  JOURNAL   Elife 7, e40958 (2018)
   PUBMED   30234487
  REMARK    GeneRIF: the ubiquitin-conjugating enzymes UBE2G1 and UBE2D3
            cooperatively promote the K48-linked polyubiquitination of
            CRL4(CRBN) neomorphic substrates
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4167)
  AUTHORS   Choi,Y.S., Lee,Y.J., Lee,S.Y., Shi,L., Ha,J.H., Cheong,H.K.,
            Cheong,C., Cohen,R.E. and Ryu,K.S.
  TITLE     Differential ubiquitin binding by the acidic loops of Ube2g1 and
            Ube2r1 enzymes distinguishes their Lys-48-ubiquitylation activities
  JOURNAL   J Biol Chem 290 (4), 2251-2263 (2015)
   PUBMED   25471371
  REMARK    GeneRIF: ubiquitin binding by the acidic loops of Ube2g1 and Ube2r1
            enzymes distinguishes their Lys-48-ubiquitylation activities
REFERENCE   5  (bases 1 to 4167)
  AUTHORS   Shibata,E., Abbas,T., Huang,X., Wohlschlegel,J.A. and Dutta,A.
  TITLE     Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G
            ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase
            complex
  JOURNAL   Mol Cell Biol 31 (15), 3136-3145 (2011)
   PUBMED   21628527
  REMARK    GeneRIF: study reports that UBCH8 and UBE2G1 and UBE2G2 cooperate
            with CRL4Cdt2 in promoting the polyubiquitylation and subsequent
            degradation of p21 and Cdt1, respectively
REFERENCE   6  (bases 1 to 4167)
  AUTHORS   Huang,L., Kinnucan,E., Wang,G., Beaudenon,S., Howley,P.M.,
            Huibregtse,J.M. and Pavletich,N.P.
  TITLE     Structure of an E6AP-UbcH7 complex: insights into ubiquitination by
            the E2-E3 enzyme cascade
  JOURNAL   Science 286 (5443), 1321-1326 (1999)
   PUBMED   10558980
REFERENCE   7  (bases 1 to 4167)
  AUTHORS   Moynihan,T.P., Ardley,H.C., Nuber,U., Rose,S.A., Jones,P.F.,
            Markham,A.F., Scheffner,M. and Robinson,P.A.
  TITLE     The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with
            RING finger/IBR motif-containing domains of HHARI and H7-AP1
  JOURNAL   J Biol Chem 274 (43), 30963-30968 (1999)
   PUBMED   10521492
REFERENCE   8  (bases 1 to 4167)
  AUTHORS   Katsanis,N. and Fisher,E.M.
  TITLE     Identification, expression, and chromosomal localization of
            ubiquitin conjugating enzyme 7 (UBE2G2), a human homologue of the
            Saccharomyces cerevisiae ubc7 gene
  JOURNAL   Genomics 51 (1), 128-131 (1998)
   PUBMED   9693041
REFERENCE   9  (bases 1 to 4167)
  AUTHORS   Watanabe,T.K., Kawai,A., Fujiwara,T., Maekawa,H., Hirai,Y.,
            Nakamura,Y. and Takahashi,E.
  TITLE     Molecular cloning of UBE2G, encoding a human skeletal
            muscle-specific ubiquitin-conjugating enzyme homologous to UBC7 of
            C. elegans
  JOURNAL   Cytogenet Cell Genet 74 (1-2), 146-148 (1996)
   PUBMED   8893823
REFERENCE   10 (bases 1 to 4167)
  AUTHORS   Chen,P., Johnson,P., Sommer,T., Jentsch,S. and Hochstrasser,M.
  TITLE     Multiple ubiquitin-conjugating enzymes participate in the in vivo
            degradation of the yeast MAT alpha 2 repressor
  JOURNAL   Cell 74 (2), 357-369 (1993)
   PUBMED   8393731
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC116910.11, BI601205.1,
            BG703127.1, BC002775.2, AC087742.9 and AI493944.1.
            
            On Aug 11, 2020 this sequence version replaced NM_003342.4.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes a member of the E2
            ubiquitin-conjugating enzyme family and catalyzes the covalent
            attachment of ubiquitin to other proteins. The protein may be
            involved in degradation of muscle-specific proteins. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853565.11785.1,
                                           SRR11853564.11524.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396981.7/ ENSP00000380178.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AC116910.11        48067-48067         c
            2-67                BI601205.1         10-75
            68-102              BG703127.1         39-73
            103-1395            BC002775.2         1-1293
            1396-3981           AC087742.9         47840-50425
            3982-4167           AI493944.1         43-228              c
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.2"
     gene            1..4167
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /note="ubiquitin conjugating enzyme E2 G1"
                     /db_xref="GeneID:7326"
                     /db_xref="HGNC:HGNC:12482"
                     /db_xref="MIM:601569"
     exon            1..405
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /inference="alignment:Splign:2.1.0"
     CDS             360..872
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /EC_number="2.3.2.23"
                     /note="ubiquitin-conjugating enzyme E2G 1 (homologous to
                     C. elegans UBC7); ubiquitin-conjugating enzyme E2G 1 (UBC7
                     homolog, C. elegans); ubiquitin-conjugating enzyme E2G 1
                     (UBC7 homolog, yeast); ubiquitin carrier protein G1;
                     ubiquitin-protein ligase G1; E2 ubiquitin-conjugating
                     enzyme G1; ubiquitin conjugating enzyme E2G 1"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 G1"
                     /protein_id="NP_003333.1"
                     /db_xref="CCDS:CCDS32532.1"
                     /db_xref="GeneID:7326"
                     /db_xref="HGNC:HGNC:12482"
                     /db_xref="MIM:601569"
                     /translation="MTELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIG
                     PPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGEDKY
                     GYEKPEERWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARC
                     VRKSQETAFE"
     exon            406..508
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /inference="alignment:Splign:2.1.0"
     exon            509..606
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /inference="alignment:Splign:2.1.0"
     exon            607..785
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /inference="alignment:Splign:2.1.0"
     exon            786..909
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /inference="alignment:Splign:2.1.0"
     exon            910..4167
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1375..1380
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /note="hexamer: ATTAAA"
     polyA_site      1398
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /note="major polyA site"
     regulatory      1975..1980
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /note="hexamer: AATATA"
     polyA_site      1995
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
     regulatory      4143..4148
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
                     /note="hexamer: AATAAA"
     polyA_site      4167
                     /gene="UBE2G1"
                     /gene_synonym="E217K; UBC7; UBE2G"
ORIGIN      
        1 agtctcagcg cgcctgcgcc gagcggccct gcgcgcagtg aggcagtggc gggggaaggc
       61 accggtgggg ccgacgggcg ggttgaagga gggaagcggg cgagcgaagt cccagtgcgc
      121 gccgcggcag cccgggcacc ctccccttcc gggcgtgagt cgctgtgaaa agagctgaag
      181 cgagcggact cgcaccggca gcgaggcgcc gctcccgccg cctcagcccg gccttcctcg
      241 gctccggcgc tccggtcgcg gggcccgggt tcctcggcac accccgctcc agccgccccc
      301 agagcctgtc cccagccctt cggaagcccc ggcgccagcc cgggccctcg gcagggagga
      361 tgacggagct gcagtcggca ctgctactgc gaagacagct ggcagaactc aacaaaaatc
      421 cagtggaagg cttttctgca ggtttaatag atgacaatga tctctaccga tgggaagtcc
      481 ttattattgg ccctccagat acactttatg aaggtggtgt ttttaaggct catcttactt
      541 tcccaaaaga ttatcccctc cgacctccta aaatgaaatt cattacagaa atctggcacc
      601 caaatgttga taaaaatggt gatgtgtgca tttctattct tcatgagcct ggggaagata
      661 agtatggtta tgaaaagcca gaggaacgct ggctccctat ccacactgtg gaaaccatca
      721 tgattagtgt catttctatg ctggcagacc ctaatggaga ctcacctgct aatgttgatg
      781 ctgcgaaaga atggagggaa gatagaaatg gagaatttaa aagaaaagtt gcccgctgtg
      841 taagaaaaag ccaagagact gcttttgagt gacatttatt tagcagctag taacttcact
      901 tatttcaggg tctccaattg agaaacatgg cactgttttt cctgcactct acccacctat
      961 tgctggactt ctgttgtaca agttggcaaa cactggctgg aactgggctg caataaaaca
     1021 tgccagttat caatgctgac aagagcctaa caagtgccaa cttacagatg attacgcatt
     1081 ttgaattcta atgaactgtt ttaaccttca ggaagaattg taaagacctg tacatagcac
     1141 aacatgatcc ggataatata tatactgttc atgtacatcc acaaatacac cttgtaccaa
     1201 ataatgcttt cttgtagtag aataagaatc gtgtaaattc taagagattt tagcaggttt
     1261 tctttcctat tcattgtttc ttatcagttt aaaaggattc ctttaagcat gtcagatgaa
     1321 aagcaattag gattaaaagt ttccatttaa tttcccttaa acccttgagg cttcattaaa
     1381 ctcttttcac ttactaaact tttgtatctt ctttgttttg acacactccc ctttgctttt
     1441 atctcttacc tgccagaatg ttctcaaatg atttagttca aatactgaaa tacttaatga
     1501 gcaattactt gatttttaat gatgacttcg aaggagtcat cactaggtgc tttgtccttt
     1561 ttgtattcta gttgcaccca cctcttggat tggatatagc aataacattt attggccgtt
     1621 gtgagctctt gatcccagtc attacccctg agaactaaaa atagatggtt cttaattcaa
     1681 cttactgaaa atttccccaa acaatagcaa atctgacttt tccctcttca gttgcctggt
     1741 attaaggttg gataaatgaa gcatgcacag ctacaggctt tctacttaac ttctgggttt
     1801 gctattacaa atcctattta ctctcatacc cttctcctta gtccttcata tttctctgcc
     1861 tctattcttc tatactgcag atttttctca cctattgtac aaagaaattg cgatgtatat
     1921 tttcatgtaa tttgattttg gaattctgtc accttatgta gtgagttctt ccaaaatata
     1981 attttttttc aataattgtc aagttgttgg cttttattgt attgaatgaa ggctataata
     2041 ctgagtgcca gagaagtggt ttaggaaaat ctcaggttga ttccttatgc aaatgaactt
     2101 ttaatacttg aaaatcacat ggccatggca gtatatgtat ttggttctat ctagattctt
     2161 ctgtgaatct aaaagcatta caggggtaaa tgctttgcta tttgacgtat agatcccgtc
     2221 actaacaata gtacacttgg atgtgattaa tgtttgagct tcaatatatt tcatatcata
     2281 cagttttcta aaacaacttc agcaaatggt aaaatgaaca tgtgcagtgt taaaggcagg
     2341 ccttaggctc cttcatgttt gttgtgaggt tgtgtgtggg aagtagtctt tggcttataa
     2401 gggatagaac ttgagacagt agcagatggg acatggtgtt tgattgtgag aatcagtgag
     2461 aattcgtgca tctctgctct gtggggtttg gagaaatgct ttggcagaag agtgaaagaa
     2521 ctcctgccaa gagcccagac ctctacaaac gttgtatgtc cttttttaag cagaaataaa
     2581 atggttgagg atgtagtcac agtagagagt gatttttttc taagtccctg tcctctactc
     2641 tgaagcgtta taaaaacctg taaacattat acaaacccac aaaccttata gaaactcgta
     2701 agtgtgttgt gactggaaat tgattcatta gaacccagtt ttctttaaga actttgtgac
     2761 ttggtttttt ttttcctttt ccaaagactg taaaaatagt tgccccaaaa tgtcagcact
     2821 gcacaccctc cagggacttg gaatacaatc ctttttactt tttttttttt ttttttaaga
     2881 aactgggtct ctctgtcacc caggctggag tgcagtggca acgatcatag ctaactgcag
     2941 ctttgacctc ctgggctcaa gtgatcctcc tgcctcagcc tcctgagtag ctaggactac
     3001 aggtgtatgc caccatgcct ggctaataca aaaaaattct tttaagagat gggatccctc
     3061 ccttttaaaa tcagaacttg ttcacatggt ggttgcttgt ggcaaaacgg agttcaaatt
     3121 ttgctctcct attgctataa ttctgctagc aatctgttga ggtgaaactt gggatctgac
     3181 tcttcagcaa gcagcaaatg acctagtaac tcagggacaa ctatttttga actttaagtg
     3241 ccactttaat gcagttagtt tgataaaacc atgtgggttt tttttttagg gctagctcta
     3301 cgggagtgga agtgagagcc aggcatgagt gcgtctccac atgcttttcc acctgccctg
     3361 agtgtgttac atactgaaac aggcctacat agatgttaca acttcccttc ctctgtcgga
     3421 gatgtcatct gtgcctttct cagtgttcat ctgataatgt aaatttaaat gcctctacat
     3481 ttgatacgaa acccacattc aggtgacact gaacgaggtg gcttttgtcc caccagtgcc
     3541 tcatcagtgt gaggcgattc ctctctgctt taggaaaatg atttttcccc ctaaacttgt
     3601 gccaaccatc aacaacatct ccatagatct tatggattgt agaactgttg gctgtttcct
     3661 aaatttattc caagttctcg tagaggcata tagatttcag tctgtgcttg tatgggatag
     3721 atgatctgag tggctttctg gcctcttttt tgagtttaaa atccatatga ggttgacgtg
     3781 tcatactaag gtaacatgtt tgtgaggtta ttccactagt actgtgatca cgtgggtgtc
     3841 agtatcttta acggccttca ttcttggttg tgagatttta tttgatatgc ccactcaccc
     3901 tcgacgaatc tgcccgcttt gggctgtggt gcctgtgtat ctttgcccgt ctggtctcca
     3961 gttggtggaa ttaccttttt tgtactgcca cttctcagca tctttgaaat ttgacataat
     4021 gttgcttcat ttcagttttt ttagttctgt aatttgttga ttgtatttaa ctatgtgagt
     4081 tctgttgtga tgtttactgt attgtaaagc acctcattca tgtgatgagt gctctataaa
     4141 tcaataaatg atgacttaga ggctgta
//
