LOCUS       NM_080678               2137 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 F (putative) (UBE2F),
            transcript variant 1, mRNA.
ACCESSION   NM_080678
VERSION     NM_080678.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Zhou,L., Dong,C., Xu,Z., Wang,X., Zhang,L., Chen,S., Chen,J. and
            Zhu,Y.
  TITLE     NEDD8-conjugating enzyme E2 UBE2F confers radiation resistance by
            protecting lung cancer cells from apoptosis
  JOURNAL   J Zhejiang Univ Sci B 22 (11), 959-965 (2021)
   PUBMED   34783226
  REMARK    GeneRIF: NEDD8-conjugating enzyme E2 UBE2F confers radiation
            resistance by protecting lung cancer cells from apoptosis.
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Zhou,L., Zhu,J., Chen,W., Jiang,Y., Hu,T., Wang,Y., Ye,X., Zhan,M.,
            Ji,C., Xu,Z., Wang,X., Gu,Y. and Jia,L.
  TITLE     Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum protects
            lung cancer cells from apoptosis and confers to
            platinum-insensitivity
  JOURNAL   Cell Death Dis 11 (11), 975 (2020)
   PUBMED   33184273
  REMARK    GeneRIF: Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum
            protects lung cancer cells from apoptosis and confers to
            platinum-insensitivity.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2137)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 2137)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2137)
  AUTHORS   Zhou,W., Xu,J., Tan,M., Li,H., Li,H., Wei,W. and Sun,Y.
  TITLE     UBE2M Is a Stress-Inducible Dual E2 for Neddylation and
            Ubiquitylation that Promotes Targeted Degradation of UBE2F
  JOURNAL   Mol Cell 70 (6), 1008-1024 (2018)
   PUBMED   29932898
  REMARK    GeneRIF: Study establishes a negative regulatory axis between two
            neddylation E2s with UBE2M ubiquitylating UBE2F, and two CRLs with
            CRL3 inactivating CRL5.
REFERENCE   6  (bases 1 to 2137)
  AUTHORS   Zhou,W., Xu,J., Li,H., Xu,M., Chen,Z.J., Wei,W., Pan,Z. and Sun,Y.
  TITLE     Neddylation E2 UBE2F Promotes the Survival of Lung Cancer Cells by
            Activating CRL5 to Degrade NOXA via the K11 Linkage
  JOURNAL   Clin Cancer Res 23 (4), 1104-1116 (2017)
   PUBMED   27591266
  REMARK    GeneRIF: By ubiquitylating and degrading NOXA through activating
            CRL5, UBE2F selectively promotes lung cancer cell survival and
            could, therefore, serve as a novel cancer target
REFERENCE   7  (bases 1 to 2137)
  AUTHORS   Monda,J.K., Scott,D.C., Miller,D.J., Lydeard,J., King,D.,
            Harper,J.W., Bennett,E.J. and Schulman,B.A.
  TITLE     Structural conservation of distinctive N-terminal
            acetylation-dependent interactions across a family of mammalian
            NEDD8 ligation enzymes
  JOURNAL   Structure 21 (1), 42-53 (2013)
   PUBMED   23201271
  REMARK    GeneRIF: Distinct preferences of UBC12 and UBE2F peptides for
            inhibiting different DCNLs, including the oncogenic DCNL1.
REFERENCE   8  (bases 1 to 2137)
  AUTHORS   Stanley,D.J., Bartholomeeusen,K., Crosby,D.C., Kim,D.Y., Kwon,E.,
            Yen,L., Cartozo,N.C., Li,M., Jager,S., Mason-Herr,J., Hayashi,F.,
            Yokoyama,S., Krogan,N.J., Harris,R.S., Peterlin,B.M. and Gross,J.D.
  TITLE     Inhibition of a NEDD8 Cascade Restores Restriction of HIV by
            APOBEC3G
  JOURNAL   PLoS Pathog 8 (12), e1003085 (2012)
   PUBMED   23300442
REFERENCE   9  (bases 1 to 2137)
  AUTHORS   Huang,D.T., Ayrault,O., Hunt,H.W., Taherbhoy,A.M., Duda,D.M.,
            Scott,D.C., Borg,L.A., Neale,G., Murray,P.J., Roussel,M.F. and
            Schulman,B.A.
  TITLE     E2-RING expansion of the NEDD8 cascade confers specificity to
            cullin modification
  JOURNAL   Mol Cell 33 (4), 483-495 (2009)
   PUBMED   19250909
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX421077.2, BC010549.1, AK290063.1, AK294107.1 and CN370973.1.
            
            On Feb 27, 2020 this sequence version replaced NM_080678.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). Both variants 1 and 2 encode the same isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK290063.1, BC010549.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000272930.9/ ENSP00000272930.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                BX421077.2         188-231
            45-1398             BC010549.1         1-1354
            1399-1527           AK290063.1         1379-1507
            1528-1600           AK294107.1         1409-1481
            1601-2137           CN370973.1         167-703
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.3"
     gene            1..2137
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /note="ubiquitin conjugating enzyme E2 F (putative)"
                     /db_xref="GeneID:140739"
                     /db_xref="HGNC:HGNC:12480"
                     /db_xref="MIM:617700"
     exon            1..119
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            120..253
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..693
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /EC_number="2.3.2.34"
                     /note="isoform 1 is encoded by transcript variant 1;
                     NEDD8-conjugating enzyme UBE2F; NEDD8 protein ligase
                     UBE2F; NEDD8-conjugating enzyme 2; NEDD8 carrier protein
                     UBE2F; ubiquitin-conjugating enzyme E2F (putative);
                     RING-type E3 NEDD8 transferase UBE2F"
                     /codon_start=1
                     /product="NEDD8-conjugating enzyme UBE2F isoform 1"
                     /protein_id="NP_542409.1"
                     /db_xref="CCDS:CCDS2523.1"
                     /db_xref="GeneID:140739"
                     /db_xref="HGNC:HGNC:12480"
                     /db_xref="MIM:617700"
                     /translation="MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAEL
                     EANLPCTCKVHFPDPNKLHCFQLTVTPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLT
                     KIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIE
                     AAEHHLRDKEDFRNKVDDYIKRYAR"
     exon            254..283
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            284..349
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            350..417
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            418..488
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            489..546
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            547..579
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            580..642
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            643..2137
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      883..888
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /note="hexamer: AATACA"
     polyA_site      901
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /note="major polyA site"
     regulatory      996..1001
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /note="hexamer: AATGAA"
     polyA_site      1008
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
     regulatory      1103..1108
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /note="hexamer: AATAAG"
     polyA_site      1128
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
     polyA_site      1272
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
     polyA_site      1319
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
     regulatory      1386..1391
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /note="hexamer: AATACA"
     polyA_site      1409
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
     regulatory      1929..1934
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
                     /note="hexamer: AATAAA"
     polyA_site      1950
                     /gene="UBE2F"
                     /gene_synonym="NCE2"
ORIGIN      
        1 acttccggtc ccgccgccgg gagccggtgc ggctgtgagg ggccgcgtct cgcagcagcc
       61 gcccggaccg ggcatggtgt tgggcgccgg gcccgcctcg cctgtctcgg ggagcccagg
      121 gtaaaggcag cagtaatgct aacgctagca agtaaactga agcgtgacga tggtctcaaa
      181 gggtcccgga cggcagccac agcgtccgac tcgactcgga gggtttctgt gagagacaaa
      241 ttgcttgtta aagaggttgc agaacttgaa gctaatttac cttgtacatg taaagtgcat
      301 tttcctgatc caaacaagct tcattgtttt cagctaacag taaccccaga tgagggttac
      361 taccagggtg gaaaatttca gtttgaaact gaagttcccg atgcgtacaa catggtgcct
      421 cccaaagtga aatgcctgac caagatctgg caccccaaca tcacagagac aggggaaata
      481 tgtctgagtt tattgagaga acattcaatt gatggcactg gctgggctcc cacaagaaca
      541 ttaaaggatg tcgtttgggg attaaactct ttgtttactg atcttttgaa ttttgatgat
      601 ccactgaata ttgaagctgc agaacatcat ttgcgggaca aggaggactt ccggaataaa
      661 gtggatgact acatcaaacg ttatgccaga tgataaaagg ggacgattgc aggcccatgg
      721 actgtgttac agtttgtctc taacatgaaa cagcaagagg tagccccctc tcccgtcctc
      781 atgctccctc tcagtcccct ggattgcccc agtcctgtga ccatgttgcc ctgaagaaga
      841 ccatcttcat gactgctcat tgtagatgga gaattcaaca taaatacagc aagaaaatgt
      901 gtttgggctt ctgaagagtt gtctgcttac cttaacatgt ttactttttt gaacttgtac
      961 tgtataggct gttggtgaaa ttcttaagaa gttgtaatga actcaaaatt gaggccagag
     1021 cttgctttcc cttttcccaa acaaaattgg ttttctgcac aagcgatgct aatgatgtgt
     1081 tcagtgtaac tcgcagattg gcaataagat acccgctaca aactgtgatt ggatgcaaaa
     1141 tctcttagct tctttcacga atgttggccc tgcctagatg ttgtgaagcc tcccagaatg
     1201 catagagtca ttcactgtag atctcttatt gaaatgcgta ttttatttaa tgtaagtata
     1261 ttttggaaca gatttgtaat ttgtacaatt caatgcttta attatttttt ctattctcat
     1321 ttagtttgta ttttcattgt atagagcaga cagaaagatg ttgggtcaag caactattga
     1381 agagaaatac aaagaaaata tgaaaggcac attattcatt ttgtccaaat gcaatgagaa
     1441 tctcactctt aaaaatcagc tcttgctttc gggtccggat gtggtgagca cattttggag
     1501 ccctttgaag ctagatttgg atgatcaaaa caaaaaggca gggagcccat tctaacatgc
     1561 tgcccagagg aaactggctg gagcctggac cagctggggt tgatgctttt gcagtggtca
     1621 tgtgattgtg acctggtagc tacttatcag agagccagac cctgctgtcc tgggagacag
     1681 gagcgatgcc tcaggaatca gcccaatgtc tgatgtcact gagactgtac ctgtggcctt
     1741 cttctgagtt tgctatggct ccaggccctg ccggtggggt gagcctccta ggccttggag
     1801 gaccaggagt caacagtggc atatgccatc ctcggccagg ttaatatact gcagaggaaa
     1861 agccctgaag agaggcaagt ggatttactc cagcatgtag acatttgaac cagtgaaatc
     1921 aaacacaaaa taaatgtctg tctagtttca tttgctgcct gcctaacact ctcaaactta
     1981 actgccagct tccttagacc tctggattcc cccatctgcc atctactggg tttagtttgt
     2041 ttttcttaat ttatagaatc tctgattgtg agatcagtct gtcacggaga cccagcaggt
     2101 gaggaatgta ggccttgctt cctctttgca cccatta
//
