LOCUS       NM_006357               1555 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 E3 (UBE2E3),
            transcript variant 1, mRNA.
ACCESSION   NM_006357 XM_941143 XM_944994 XM_944996 XM_944999 XM_945001
VERSION     NM_006357.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 1555)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1555)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1555)
  AUTHORS   Plafker,K.S., Zyla,K., Berry,W. and Plafker,S.M.
  TITLE     Loss of the ubiquitin conjugating enzyme UBE2E3 induces cellular
            senescence
  JOURNAL   Redox Biol 17, 411-422 (2018)
   PUBMED   29879550
  REMARK    GeneRIF: Cells depleted of UBE2E3 become senescent in the absence
            of overt DNA damage and have a distinct senescence-associated
            secretory phenotype, increased mitochondrial and lysosomal mass, an
            increased sensitivity to mitochondrial and lysosomal poisons, and
            an increased basal autophagic flux.
REFERENCE   6  (bases 1 to 1555)
  AUTHORS   Hay,R.T., Vuillard,L., Desterro,J.M. and Rodriguez,M.S.
  TITLE     Control of NF-kappa B transcriptional activation by signal induced
            proteolysis of I kappa B alpha
  JOURNAL   Philos Trans R Soc Lond B Biol Sci 354 (1389), 1601-1609 (1999)
   PUBMED   10582246
  REMARK    Review article
REFERENCE   7  (bases 1 to 1555)
  AUTHORS   Desterro,J.M., Rodriguez,M.S., Kemp,G.D. and Hay,R.T.
  TITLE     Identification of the enzyme required for activation of the small
            ubiquitin-like protein SUMO-1
  JOURNAL   J Biol Chem 274 (15), 10618-10624 (1999)
   PUBMED   10187858
REFERENCE   8  (bases 1 to 1555)
  AUTHORS   Ito,K., Kato,S., Matsuda,Y., Kimura,M. and Okano,Y.
  TITLE     cDNA cloning, characterization, and chromosome mapping of UBE2E3
            (alias UbcH9), encoding an N-terminally extended human
            ubiquitin-conjugating enzyme
  JOURNAL   Cytogenet Cell Genet 84 (1-2), 99-104 (1999)
   PUBMED   10343118
REFERENCE   9  (bases 1 to 1555)
  AUTHORS   Desterro,J.M., Rodriguez,M.S. and Hay,R.T.
  TITLE     SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation
  JOURNAL   Mol Cell 2 (2), 233-239 (1998)
   PUBMED   9734360
REFERENCE   10 (bases 1 to 1555)
  AUTHORS   Desterro,J.M., Thomson,J. and Hay,R.T.
  TITLE     Ubch9 conjugates SUMO but not ubiquitin
  JOURNAL   FEBS Lett 417 (3), 297-300 (1997)
   PUBMED   9409737
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB458228.1, BC092407.1 and
            BU608985.1.
            
            On Nov 23, 2018 this sequence version replaced NM_006357.3.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes a member of the E2
            ubiquitin-conjugating enzyme family. The encoded protein shares
            100% sequence identity with the mouse and rat counterparts, which
            indicates that this enzyme is highly conserved in eukaryotes.
            Multiple alternatively spliced transcript variants encoding the
            same protein have been found for this gene. [provided by RefSeq,
            Jun 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2, 3 and 4 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1025546.1,
                                           SRR3476690.940336.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000410062.9/ ENSP00000386788.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-336               DB458228.1         1-336
            337-1553            BC092407.1         301-1517
            1554-1555           BU608985.1         18-19               c
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.3"
     gene            1..1555
                     /gene="UBE2E3"
                     /gene_synonym="UBCH9; UbcM2"
                     /note="ubiquitin conjugating enzyme E2 E3"
                     /db_xref="GeneID:10477"
                     /db_xref="HGNC:HGNC:12479"
                     /db_xref="MIM:604151"
     exon            1..369
                     /gene="UBE2E3"
                     /gene_synonym="UBCH9; UbcM2"
                     /inference="alignment:Splign:2.1.0"
     exon            370..588
                     /gene="UBE2E3"
                     /gene_synonym="UBCH9; UbcM2"
                     /inference="alignment:Splign:2.1.0"
     CDS             395..1018
                     /gene="UBE2E3"
                     /gene_synonym="UBCH9; UbcM2"
                     /EC_number="2.3.2.23"
                     /note="ubiquitin-conjugating enzyme E2E 3 (homologous to
                     yeast UBC4/5); ubiquitin conjugating enzyme E2E 3;
                     ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog,
                     yeast); ubiquitin-protein ligase E3; ubiquitin carrier
                     protein E3; ubiquitin-conjugating enzyme E2-23 kDa; E2
                     ubiquitin-conjugating enzyme E3"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 E3"
                     /protein_id="NP_006348.1"
                     /db_xref="CCDS:CCDS2282.1"
                     /db_xref="GeneID:10477"
                     /db_xref="HGNC:HGNC:12479"
                     /db_xref="MIM:604151"
                     /translation="MSSDRQRSDDESPSTSSGSSDADQRDPAAPEPEEQEERKPSATQ
                     QKKNTKLSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPG
                     SVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTIS
                     KVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT"
     exon            589..639
                     /gene="UBE2E3"
                     /gene_synonym="UBCH9; UbcM2"
                     /inference="alignment:Splign:2.1.0"
     exon            640..772
                     /gene="UBE2E3"
                     /gene_synonym="UBCH9; UbcM2"
                     /inference="alignment:Splign:2.1.0"
     exon            773..920
                     /gene="UBE2E3"
                     /gene_synonym="UBCH9; UbcM2"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1555
                     /gene="UBE2E3"
                     /gene_synonym="UBCH9; UbcM2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1534..1539
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2E3"
                     /gene_synonym="UBCH9; UbcM2"
                     /note="hexamer: AATAAA"
     polyA_site      1555
                     /gene="UBE2E3"
                     /gene_synonym="UBCH9; UbcM2"
                     /note="major polyA site"
ORIGIN      
        1 ggaacttgct gactgcgcgg ccgggaggag ccgagccggg cggcggcggc gggaggctac
       61 agcgcgcggg ggtctcccgc gtcccctccg cctcgccggg agctcgcgcc ctcgcccagc
      121 cgagctccca cccccgcttt tttccgaagg cgctgggcgg cgccaccctc cggccggagc
      181 ccggcactgc acaaccccct ccgactttca atgttccaca ctccccggcc agagcctcct
      241 cggcttcttt ttttccctcc ccccccttcc cccccccaca gctgcctcca tttccttaag
      301 gaagggtttt tttctctctc cctcccccac accgtagcgg cgcgcgagcg ggccgggcgg
      361 gcggccgagt tttccaagag ataacttcac caagatgtcc agtgataggc aaaggtccga
      421 tgatgagagc cccagcacca gcagtggcag ttcagatgcg gaccagcgag acccagccgc
      481 tccagagcct gaagaacaag aggaaagaaa accttctgcc acccagcaga agaaaaacac
      541 caaactctct agcaaaacca ctgctaagtt atccactagt gctaaaagaa ttcagaagga
      601 gctagctgaa ataacccttg atcctcctcc taattgcagt gctgggccta aaggagataa
      661 catttatgaa tggagatcaa ctatacttgg tccaccgggt tctgtatatg aaggtggtgt
      721 gttttttctg gatatcacat tttcatcaga ttatccattt aagccaccaa aggttacttt
      781 ccgcaccaga atctatcact gcaacatcaa cagtcaggga gtcatctgtc tggacatcct
      841 taaagacaac tggagtcccg ctttgactat ttcaaaggtt ttgctgtcta tttgttccct
      901 tttgacagac tgcaaccctg cggatcctct ggttggaagc atagccactc agtatttgac
      961 caacagagca gaacacgaca ggatagccag acagtggacc aagagatacg caacataatt
     1021 cacataattt gtatgcagtg tgaaggagca gaaggcatct tctcactgtg ctgcaaatct
     1081 ttatagcctt tacaatacgg acttctgtgt atatgttata ctgattctac tctgctttta
     1141 tcctttggag cctgggagac tccccaaaaa ggtaaatgct atcaagagta gaactttgta
     1201 gctgtagatt agttatgttt aaaacgccta cttgcaagtc ttgcttcttt gggatatcaa
     1261 aatgtatttt gtgatgtact aaggatactg gtcctgaagt ctaccaaata ttatagtgca
     1321 ttttagccta attcattatc tgtatgaagt tataaaagta gctgtagatg gctaggaatt
     1381 atgtcatttg tattaaaccc agatctattt ctgagtatgt ggttcatgct gttgtgaaaa
     1441 atgttttacc ttttaccttt gtcagtttgt aatgagagga tttcctttta ccctttgtag
     1501 ctcagagagc acctgatgta tcatctcaaa cacaataaac atgctcctga aggca
//
