LOCUS       NM_152653               2715 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 E2 (UBE2E2),
            transcript variant 1, mRNA.
ACCESSION   NM_152653
VERSION     NM_152653.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Hong,X., Ma,N., Li,D., Zhang,M., Dong,W., Huang,J., Ci,X. and
            Zhang,S.
  TITLE     UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition
            and Nrf2-mediated antioxidant activity in ovarian cancer
  JOURNAL   Cell Death Dis 14 (2), 100 (2023)
   PUBMED   36765041
  REMARK    GeneRIF: UBE2E2 enhances Snail-mediated epithelial-mesenchymal
            transition and Nrf2-mediated antioxidant activity in ovarian
            cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2715)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2715)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2715)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   6  (bases 1 to 2715)
  AUTHORS   Pringa,E., Martinez-Noel,G., Muller,U. and Harbers,K.
  TITLE     Interaction of the ring finger-related U-box motif of a nuclear dot
            protein with ubiquitin-conjugating enzymes
  JOURNAL   J Biol Chem 276 (22), 19617-19623 (2001)
   PUBMED   11274149
REFERENCE   7  (bases 1 to 2715)
  AUTHORS   Ito,K., Adachi,S., Iwakami,R., Yasuda,H., Muto,Y., Seki,N. and
            Okano,Y.
  TITLE     N-Terminally extended human ubiquitin-conjugating enzymes (E2s)
            mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8
  JOURNAL   Eur J Biochem 268 (9), 2725-2732 (2001)
   PUBMED   11322894
REFERENCE   8  (bases 1 to 2715)
  AUTHORS   Niwa,J., Ishigaki,S., Doyu,M., Suzuki,T., Tanaka,K. and Sobue,G.
  TITLE     A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin
            ligase activity
  JOURNAL   Biochem Biophys Res Commun 281 (3), 706-713 (2001)
   PUBMED   11237715
REFERENCE   9  (bases 1 to 2715)
  AUTHORS   Moynihan,T.P., Ardley,H.C., Nuber,U., Rose,S.A., Jones,P.F.,
            Markham,A.F., Scheffner,M. and Robinson,P.A.
  TITLE     The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with
            RING finger/IBR motif-containing domains of HHARI and H7-AP1
  JOURNAL   J Biol Chem 274 (43), 30963-30968 (1999)
   PUBMED   10521492
REFERENCE   10 (bases 1 to 2715)
  AUTHORS   Kimura,M., Hattori,T., Matsuda,Y., Yoshioka,T., Sumi,N., Umeda,Y.,
            Nakashima,S. and Okano,Y.
  TITLE     cDNA cloning, characterization, and chromosome mapping of UBE2E2
            encoding a human ubiquitin-conjugating E2 enzyme
  JOURNAL   Cytogenet Cell Genet 78 (2), 107-111 (1997)
   PUBMED   9371400
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK057886.1, BC022332.1, CB851345.1 and AC116992.2.
            
            On Nov 22, 2018 this sequence version replaced NM_152653.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.220419.1,
                                           SRR1803612.17213.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396703.6/ ENSP00000379931.1
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AK057886.1         35-168
            135-1005            BC022332.1         145-1015
            1006-1500           AK057886.1         1041-1535
            1501-1726           CB851345.1         523-748
            1727-2715           AC116992.2         135808-136796
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p24.3"
     gene            1..2715
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /note="ubiquitin conjugating enzyme E2 E2"
                     /db_xref="GeneID:7325"
                     /db_xref="HGNC:HGNC:12478"
                     /db_xref="MIM:602163"
     exon            1..138
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /inference="alignment:Splign:2.1.0"
     exon            139..322
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..752
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /EC_number="2.3.2.23"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin-conjugating enzyme E2E 2 (homologous to yeast
                     UBC4/5); ubiquitin-conjugating enzyme E2E 2 (UBC4/5
                     homolog, yeast); ubiquitin-protein ligase E2; ubiquitin
                     carrier protein E2; E2 ubiquitin-conjugating enzyme E2;
                     ubiquitin conjugating enzyme E2E 2"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 E2 isoform 1"
                     /protein_id="NP_689866.1"
                     /db_xref="CCDS:CCDS2637.1"
                     /db_xref="GeneID:7325"
                     /db_xref="HGNC:HGNC:12478"
                     /db_xref="MIM:602163"
                     /translation="MSTEAQRVDDSPSTSGGSSDGDQRESVQQEPEREQVQPKKKEGK
                     ISSKTAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGG
                     VFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSI
                     CSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT"
     exon            323..373
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /inference="alignment:Splign:2.1.0"
     exon            374..506
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /inference="alignment:Splign:2.1.0"
     exon            507..654
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /inference="alignment:Splign:2.1.0"
     exon            655..2715
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1475..1480
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /note="hexamer: AATAAA"
     polyA_site      1501
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /note="major polyA site"
     regulatory      1759..1764
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /note="hexamer: ATTAAA"
     polyA_site      1781
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
     regulatory      1951..1956
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /note="hexamer: AGTAAA"
     polyA_site      1973
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
     regulatory      2689..2694
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
                     /note="hexamer: AATAAA"
     polyA_site      2715
                     /gene="UBE2E2"
                     /gene_synonym="UBCH8"
ORIGIN      
        1 ggctttcggt gactagacgg tccgcagggg acatcccgtc cctggggcct ccccagtctc
       61 cctccccctc gcgcctgggc agctctctcc cagggcttcg gctcgagcct gcgacctgca
      121 cggacacccc cccctcagga tctaaaatgt ccactgaggc acaaagagtt gatgacagtc
      181 caagcactag tggaggaagt tccgatggag atcaacgtga aagtgttcag caagaaccag
      241 aaagagaaca agttcagccc aagaaaaagg agggaaaaat atccagcaaa accgctgcta
      301 aattgtcaac tagtgctaaa agaattcaga aggaacttgc agaaatcaca ttggaccctc
      361 ctcccaactg tagtgctgga cccaaaggag acaacattta tgaatggagg tcaactatat
      421 tgggaccccc aggatctgtc tatgaaggag gggtgttctt tcttgacatt accttttcac
      481 cagactatcc gtttaaaccc cctaaggtta ccttccgaac aagaatctat cactgtaata
      541 ttaacagcca aggtgtgatc tgtctggaca tcttaaagga caactggagt ccggctttaa
      601 ctatttctaa agttctcctc tccatctgct cacttcttac agattgcaac cctgctgacc
      661 ctctggtggg cagcatcgcc acacagtaca tgaccaacag agcagagcat gaccggatgg
      721 ccagacagtg gaccaagcgg tacgccacat aggggcctgc tgcctgccgc cccgcgggac
      781 ctgtgcaagc acattcacca agtgcatcgg tagccctgcc cacccctcca gacctcggtt
      841 cttattttcc tatttttatt aaatttggaa ccattttgtg atggtatgtt gtccatcttc
      901 ccatcccagt tcttcctgcc ccccttcctc tctcccacgc tctcttttat ctctcatttt
      961 attcccttgt tgatttctgt taacttgaaa gatttgggat tttttcccac ctcatcatag
     1021 atgggaactt ttgttttcag tgcaaacaat gttggagctg taatagtaag agctttctta
     1081 caaagctttg tattactgtg tggttttgtt ttttttgttg ttgtttattt gattttgatt
     1141 tttttttctt ttatgtgatc tttgggaaaa cacattcaga attatatctc gtttctactt
     1201 aaatgtagtg cttagggtta attttttgta ctgaagtctt tattggtggg tgcatgctac
     1261 tgggaacaag tttttgtaca aaagcttcaa tcagaatcac tgtgcattac tgagactctg
     1321 tttatcacta gccttctgtc cctcccgcag aagactgttg gattgaacaa aataatatgt
     1381 attttgattt acttaaagtg cttgtaaatt tcttagggac ctgccacttt tgactgtgga
     1441 tcagttgatg tacacttgta ttattaaagc actcaataaa tcactgtggc tgataactgc
     1501 acttctggta acccgacatt tgctttgtgt cctggtgacc gctgtagccc tacgtgcagt
     1561 gaggcttgtc taattcaatt acaggttcaa gtgtattttt catctcaaac ctctaatatt
     1621 tctttggagt tgagttgctt agcatgtgga atttctccag ctgtcagtag cctgatgatt
     1681 ttatggttgt tatagtaaat tgctatcatt ttacatattg actgggcatt ctttctgtta
     1741 cagccttctt tatcaacaat taaaatatgt aactccaaaa atagccttct tgtgaaagtg
     1801 aagtctgagc taatcattgt gttcagccac tttcaacttt atttctctca aaattgttac
     1861 agtgattgcc ctcaagcttt gtattgttca tttttgttgt tgttgttgtt gttgttgctg
     1921 ctcttttttc tccatgattt tagcaaaaga agtaaactca actgtattgg tcacggtcag
     1981 cccattcaat gacaagggtg tctgtctggc ttcaccttca ggctatttta gtgtgcagct
     2041 ataggaggca gaggaaacgt gggtggtgta gtttctaaaa cacgctgact tgctgattga
     2101 taattcaggg ttattagtgt aacaaagcgt agtggaaact cctactcctg ctgcaccata
     2161 cagttacaga ccctaccata ctcttggtct catttaagga accattaaca ccttcactgc
     2221 tgaaaagcag gatttataag cataagtcat ccacctctaa atattttaac atttaaaatg
     2281 cctgattcaa aagatacagt ttttgttgtt tttaactagt taaaaataaa tcctaagaaa
     2341 atatatttaa atattgcaaa tgccacttct aaaccaaaat acctgagaat attttttgtc
     2401 tttttaataa cgattttacc attcatttta tttaaaaaca tttctcagca tagcagggca
     2461 ctgtatggtc tctgatgcac tactcaaccc tctagttgta gtagagggag ccacagacag
     2521 tacataaatg cattgggcag agctatattc cagtgaaact ttatttacca ccataggcat
     2581 gggatttggc ccatgggttg tagtttgcca atccctgaca cagaccaaca tagactgggg
     2641 gctggatgaa aaacaaataa cgatgtgtca tttctttctt taacataaaa taaattcaat
     2701 atggtacaac actga
//
