LOCUS       NM_003341               1783 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 E1 (UBE2E1),
            transcript variant 1, mRNA.
ACCESSION   NM_003341
VERSION     NM_003341.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Anandapadamanaban,M., Kyriakidis,N.C., Csizmok,V., Wallenhammar,A.,
            Espinosa,A.C., Ahlner,A., Round,A.R., Trewhella,J., Moche,M.,
            Wahren-Herlenius,M. and Sunnerhagen,M.
  TITLE     E3 ubiquitin-protein ligase TRIM21-mediated lysine capture by
            UBE2E1 reveals substrate-targeting mode of a ubiquitin-conjugating
            E2
  JOURNAL   J Biol Chem 294 (30), 11404-11419 (2019)
   PUBMED   31160341
  REMARK    GeneRIF: found that critical UBE2E1 residues involved in the
            capture of the TRIM21 substrate lysine are conserved in
            ubiquitin-conjugating E2s, whereas residues critical for
            SUMOylation are not conserved
REFERENCE   3  (bases 1 to 1783)
  AUTHORS   Pasupala,N., Morrow,M.E., Que,L.T., Malynn,B.A., Ma,A. and
            Wolberger,C.
  TITLE     OTUB1 non-catalytically stabilizes the E2 ubiquitin-conjugating
            enzyme UBE2E1 by preventing its autoubiquitination
  JOURNAL   J Biol Chem 293 (47), 18285-18295 (2018)
   PUBMED   30282802
  REMARK    GeneRIF: OTUB1 depletion dramatically destabilizes the
            E2-conjugating enzyme UBE2E1 in both mouse and human OTUB1 knockout
            cell lines.
REFERENCE   4  (bases 1 to 1783)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 1783)
  AUTHORS   Takeuchi,T., Iwahara,S., Saeki,Y., Sasajima,H. and Yokosawa,H.
  TITLE     Link between the ubiquitin conjugation system and the ISG15
            conjugation system: ISG15 conjugation to the UbcH6 ubiquitin E2
            enzyme
  JOURNAL   J Biochem 138 (6), 711-719 (2005)
   PUBMED   16428300
  REMARK    GeneRIF: isolated ISG15-modified and unmodified UbcH6 proteins, and
            analyzed their abilities to form thioester intermediates with
            ubiquitin
REFERENCE   6  (bases 1 to 1783)
  AUTHORS   Kramer,E.R., Scheuringer,N., Podtelejnikov,A.V., Mann,M. and
            Peters,J.M.
  TITLE     Mitotic regulation of the APC activator proteins CDC20 and CDH1
  JOURNAL   Mol Biol Cell 11 (5), 1555-1569 (2000)
   PUBMED   10793135
REFERENCE   7  (bases 1 to 1783)
  AUTHORS   Lukas,C., Sorensen,C.S., Kramer,E., Santoni-Rugiu,E., Lindeneg,C.,
            Peters,J.M., Bartek,J. and Lukas,J.
  TITLE     Accumulation of cyclin B1 requires E2F and cyclin-A-dependent
            rearrangement of the anaphase-promoting complex
  JOURNAL   Nature 401 (6755), 815-818 (1999)
   PUBMED   10548110
REFERENCE   8  (bases 1 to 1783)
  AUTHORS   Anan,T., Nagata,Y., Koga,H., Honda,Y., Yabuki,N., Miyamoto,C.,
            Kuwano,A., Matsuda,I., Endo,F., Saya,H. and Nakao,M.
  TITLE     Human ubiquitin-protein ligase Nedd4: expression, subcellular
            localization and selective interaction with ubiquitin-conjugating
            enzymes
  JOURNAL   Genes Cells 3 (11), 751-763 (1998)
   PUBMED   9990509
REFERENCE   9  (bases 1 to 1783)
  AUTHORS   Nuber,U., Schwarz,S., Kaiser,P., Schneider,R. and Scheffner,M.
  TITLE     Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7
            (E2-F1) and characterization of their interaction with E6-AP and
            RSP5
  JOURNAL   J Biol Chem 271 (5), 2795-2800 (1996)
   PUBMED   8576257
REFERENCE   10 (bases 1 to 1783)
  AUTHORS   Chen,P., Johnson,P., Sommer,T., Jentsch,S. and Hochstrasser,M.
  TITLE     Multiple ubiquitin-conjugating enzymes participate in the in vivo
            degradation of the yeast MAT alpha 2 repressor
  JOURNAL   Cell 74 (2), 357-369 (1993)
   PUBMED   8393731
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB478686.1, AK314854.1 and
            AC124914.3.
            
            On May 17, 2019 this sequence version replaced NM_003341.4.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes a member of the E2
            ubiquitin-conjugating enzyme family. Three alternatively spliced
            transcript variants encoding distinct isoforms have been found for
            this gene. [provided by RefSeq, Jan 2011].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.650674.1,
                                           SRR3476690.1086990.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000306627.8/ ENSP00000303709.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                DB478686.1         67-95
            30-749              AK314854.1         1-720
            750-1783            AC124914.3         35784-36817
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p24.2"
     gene            1..1783
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /note="ubiquitin conjugating enzyme E2 E1"
                     /db_xref="GeneID:7324"
                     /db_xref="HGNC:HGNC:12477"
                     /db_xref="MIM:602916"
     exon            1..134
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /inference="alignment:Splign:2.1.0"
     exon            135..319
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /inference="alignment:Splign:2.1.0"
     CDS             168..749
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /EC_number="2.3.2.23"
                     /EC_number="2.3.2.24"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin-conjugating enzyme E2E 1 (homologous to yeast
                     UBC4/5); ubiquitin-conjugating enzyme E2E 1 (UBC4/5
                     homolog, yeast); ubiquitin-protein ligase E1; ubiquitin
                     carrier protein E1; E2 ubiquitin-conjugating enzyme E1;
                     (E3-independent) E2 ubiquitin-conjugating enzyme E1;
                     ubiquitin conjugating enzyme E2E 1"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 E1 isoform 1"
                     /protein_id="NP_003332.1"
                     /db_xref="CCDS:CCDS2638.1"
                     /db_xref="GeneID:7324"
                     /db_xref="HGNC:HGNC:12477"
                     /db_xref="MIM:602916"
                     /translation="MSDDDSRASTSSSSSSSSNQQTEKETNTPKKKESKVSMSKNSKL
                     LSTSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITF
                     TPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCN
                     PADPLVGSIATQYMTNRAEHDRMARQWTKRYAT"
     exon            320..370
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /inference="alignment:Splign:2.1.0"
     exon            371..503
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /inference="alignment:Splign:2.1.0"
     exon            504..651
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /inference="alignment:Splign:2.1.0"
     exon            652..1783
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1428..1433
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /note="hexamer: AATAAA"
     regulatory      1438..1443
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /note="hexamer: ATTAAA"
     polyA_site      1448
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /note="major polyA site"
     regulatory      1452..1457
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
                     /note="hexamer: AATAAA"
     polyA_site      1459
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
     polyA_site      1475
                     /gene="UBE2E1"
                     /gene_synonym="UBCH6"
ORIGIN      
        1 attgcctgtt taatagttgc tgttgctgca cttccgcttc tctcccagcg agagagagac
       61 acgagtggcc aggcccagcc gcagccgcag cagcagccgc cgcggcggca cggaggagcc
      121 agacacaaag agaggggctg tttgcggggt ggggtggggg gttcgctatg tcggatgacg
      181 attcgagggc cagcaccagc tcctcctcat cttcgtcttc caaccagcaa accgagaaag
      241 aaacaaacac ccccaagaag aaggagagta aagtcagcat gagcaaaaac tccaaactcc
      301 tctccaccag cgccaagaga attcagaagg agctggcgga catcacttta gaccctccac
      361 ctaattgcag tgctggtccc aaaggcgata acatctatga atggagatca accattctag
      421 ggcctccagg atccgtgtat gagggtggtg tattctttct cgatatcact tttacaccag
      481 aatatccctt caagcctcca aaggttacat ttcggacaag aatctatcat tgtaatatta
      541 acagtcaagg tgttatttgc ttggacatat tgaaagataa ttggagtcca gcactaacca
      601 tttctaaagt cctcctttct atctgctcac ttcttacaga ctgtaatcct gccgacccct
      661 tggtgggaag tattgccact cagtatatga ccaacagagc agaacatgac agaatggcca
      721 gacagtggac caagagatac gctacataaa ttggggtttc acaattctta cattatttgt
      781 ctgtcacaga agagagctgc ttatgatttt gaaggggtca gggagggtgg gagttggtaa
      841 agagtagggt atttctataa cagatattat tcagtcttat ttcctaagat tttgttgtaa
      901 cttaaggtat cttgctacag tagacagaat tggtaatagc aacttttaaa attgtcatta
      961 gttctgcaat attagctgaa atgtagtaca gaaaagaatg tacatttaga catttgggtt
     1021 cagttgcttg tagtctgtaa atttaaaaca gcttaatttg gtacaggtta cacatatggc
     1081 catttatgta aagtccctct aagactacat actttttgtt taaaacaaaa ttggaatttg
     1141 ttttcccttc ttggaaggga acattgatat ttaacagagt ttttagagat tgtcatctca
     1201 tatatataaa atggacacgt ggctataaaa caccatataa gagatgagta gtgcgtttta
     1261 ttttatatgc caatctactt tgtttaaaaa aggtctgaat caggacttgt gaaaacctgt
     1321 agtgaaatac cttaagctgt taactaactg taaggcgtgg aataggagtt gctcagtgga
     1381 ttggttctat gttgtggact acttaagtct gcatttgtta ctgtgctaat aaacaatatt
     1441 aaaaaccacc taataaacac tgctgtgttc atttactttt cttttgcctt ttggttgcca
     1501 ttcgcagatt cttctgaaat cgataggtat ctgcttctaa aacaagctaa aaataatgca
     1561 tatttagtag gtattagtta gttacctata ttaggatctg tactttacac agctgtagat
     1621 gaggtatcct agaaaaggtt aaataatcta ccccatttag gcttcaaaga cgaaccctac
     1681 tgcatctttt taaaagcatt ttctgacttg cagatgctgt agtagcaagt acatgagaaa
     1741 tgggttccgt catggataaa ttggtaccct gccttagggg gca
//
