LOCUS       NM_015983               3982 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 D4 (UBE2D4), mRNA.
ACCESSION   NM_015983
VERSION     NM_015983.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 3982)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3982)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3982)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 3982)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   7  (bases 1 to 3982)
  AUTHORS   David,Y., Ziv,T., Admon,A. and Navon,A.
  TITLE     The E2 ubiquitin-conjugating enzymes direct polyubiquitination to
            preferred lysines
  JOURNAL   J Biol Chem 285 (12), 8595-8604 (2010)
   PUBMED   20061386
REFERENCE   8  (bases 1 to 3982)
  AUTHORS   Markson,G., Kiel,C., Hyde,R., Brown,S., Charalabous,P., Bremm,A.,
            Semple,J., Woodsmith,J., Duley,S., Salehi-Ashtiani,K., Vidal,M.,
            Komander,D., Serrano,L., Lehner,P. and Sanderson,C.M.
  TITLE     Analysis of the human E2 ubiquitin conjugating enzyme protein
            interaction network
  JOURNAL   Genome Res 19 (10), 1905-1911 (2009)
   PUBMED   19549727
REFERENCE   9  (bases 1 to 3982)
  AUTHORS   van Wijk,S.J., de Vries,S.J., Kemmeren,P., Huang,A., Boelens,R.,
            Bonvin,A.M. and Timmers,H.T.
  TITLE     A comprehensive framework of E2-RING E3 interactions of the human
            ubiquitin-proteasome system
  JOURNAL   Mol Syst Biol 5, 295 (2009)
   PUBMED   19690564
  REMARK    Erratum:[Mol Syst Biol. 2009;5:317]
REFERENCE   10 (bases 1 to 3982)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BG499454.1, BC004104.2, AC004985.2 and AW014299.1.
            
            On Nov 22, 2018 this sequence version replaced NM_015983.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.17829.1,
                                           SRR1660805.230028.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000222402.8/ ENSP00000222402.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 BG499454.1         2-9
            9-1406              BC004104.2         1-1398
            1407-3675           AC004985.2         9571-11839          c
            3676-3982           AW014299.1         17-323              c
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p13"
     gene            1..3982
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /note="ubiquitin conjugating enzyme E2 D4"
                     /db_xref="GeneID:51619"
                     /db_xref="HGNC:HGNC:21647"
     exon            1..121
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..541
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /EC_number="2.3.2.23"
                     /note="ubiquitin-conjugating enzyme HBUCE1;
                     ubiquitin-protein ligase D4; ubiquitin carrier protein D4;
                     E2 ubiquitin-conjugating enzyme D4; ubiquitin conjugating
                     enzyme E2D 4 (putative); ubiquitin conjugating enzyme E2
                     D4 (putative)"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 D4"
                     /protein_id="NP_057067.1"
                     /db_xref="CCDS:CCDS5474.1"
                     /db_xref="GeneID:51619"
                     /db_xref="HGNC:HGNC:21647"
                     /translation="MALKRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSP
                     YQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKV
                     LLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLAREWTQKYAM"
     exon            122..185
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            186..217
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            218..295
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            296..401
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            402..495
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            496..3982
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1383..1388
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /note="hexamer: AATAAA"
     polyA_site      1413
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /note="major polyA site"
     regulatory      3949..3954
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /note="hexamer: AACAAA"
     regulatory      3958..3963
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
                     /note="hexamer: TATAAA"
     polyA_site      3966
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
     polyA_site      3982
                     /gene="UBE2D4"
                     /gene_synonym="HBUCE1"
ORIGIN      
        1 gcggctcccg gcgtgcagct tggtggcggc tgagccggca gcgggccgcc tcaggcagcc
       61 ccggccgggc cgcccgggtc cccggcagcg gggtaggatg gcgctaaagc ggatccagaa
      121 ggaattaacc gacttgcaga gggatcctcc tgcccagtgt tctgcaggac ctgtcggtga
      181 tgacttgttc cactggcagg ccaccatcat gggcccgaat gacagtcctt accaaggagg
      241 tgttttcttc ctgaccatcc actttcctac agattacccg ttcaagcccc caaaggttgc
      301 tttcacaacc aaaatttatc accctaatat caacagcaat ggcagcatct gccttgatat
      361 cctgcggtct cagtggtctc cagcgttgac tgtgtcaaaa gttctcttgt ccatctgctc
      421 gctgctctgc gaccccaacc ccgatgaccc cctggtgcca gagatagcac acacctacaa
      481 ggccgacaga gagaagtaca acagactagc aagagagtgg acacaaaaat atgctatgta
      541 agtgccttgg aggttttaca tgagacactg tccaagagaa gctggcagag aggtcttccc
      601 ttaaaacttt gggctgttgg ctgagccatt caaagagcat catctgttct tcaaacaaat
      661 gttggtcacc cactctctcc agctgcagca tgttggtgcc attttcagca attacggctt
      721 tgacagtgcc acctctttga tgccaaatca gcaaccattg ttgttatgat ctgcagtctt
      781 cctggtgaca ctggaatctc tctctctgcc gcctcagttt gtctgctggt ctcttggggg
      841 gccaggccct gcacgtctct cctacccggc ctcaaatggt gctgctgccc atgatggtac
      901 cacaccaggg cctcagcctg gcccctcacc acataccctt tgccttttag aactcagtgc
      961 catcctgggt gcccagggca gagcaggctt tgttcgcacc tcatctgctg cagaaccaca
     1021 tcctgaggag tctcagctta tcctggaggg aattgggaac agtgtcactg ggaagtgaag
     1081 gcctagccct gtggcttcca ccagtctcct cctgcagtgc cacgtggtgg catttctcgc
     1141 ctcacaccaa gaagcagcaa gtggaaaatt tcaggataca aagcacataa caccccataa
     1201 gagatgatta tgtttttaga agcaagagca aaattatgaa acctctagag atttgggtca
     1261 tgttactcca tttgatgaag attctcacta ccgcccgctc ctcccatagg agcctacact
     1321 aagtccaagt gtgagccatt cacagactag aacacaagga gggagagaga ctcttaaacg
     1381 taaataaaaa tgcaattcac tcacaactct aaatgataat tgacatttca gcctctttct
     1441 tttaggcaaa acagaagata cacactatta acagttattt gaagccttaa attacaatag
     1501 cctgttaggt gatcggcttt ctgccttggc tttctgatgc agcacatttt aagatttgcc
     1561 tttaaagctt tgagccttga gattaggtta gttaggaaga ggacaacaaa gggaagagat
     1621 tattctaata aaacttccac agagggaaga aaattgagga gcttcagatc tctaaagcca
     1681 aattcaacct gatctttcta ggttggttat aaagctggtc cacttccagg aagtgatgtg
     1741 taggtgaata ggaacaaaag gtgaagaagc agtaaaactg tgaccaagtg gccagatggg
     1801 gcttttgccg gctgattgaa aggaagtcta cagacctaac ataagcagtc agccaaaggt
     1861 ggctgtggga ggttgatttc ttccagaaac ttccaagttg tggctacaaa tttattgcca
     1921 gatattttcc cactgagaat actgaggtta ttcacataat cacttcctta acaaaggaag
     1981 aagcactttt aacttgggct gcattccctt tgtgagggtc cacatttagg atcctcctgc
     2041 taccataagg ccccttttgg ggttctttgg gtgtggctgc atctcaccat gttgaggatt
     2101 gccttttttt tttttttttt tttttttttt taacacagag ccttgctctg tctcccaggc
     2161 tggagtgcag tggtgcgatc ttggctcact gcaacctctg cctcccaggt tcaagcaatt
     2221 ctcctgcctc agcctcccaa gtagccggaa ttacaggcat gtgccaccat gctgggctaa
     2281 tttttgtatt tttagtggag acggtgtttc accatgttgg ccaggctgtt cttgaactcc
     2341 tggcctcaag tgacccgccc acattggcct cccaaagtgc tgggattaca ggcgtgagcc
     2401 cccgtgccca gcaaggattg ctgttttaat tcttgtatct tcgttgcttc tttggtcttt
     2461 gcttaaacaa aacaataaag cttaatagtt ggggagaatg ctgttaccag tgtcctctag
     2521 gaaggctgtt agcatatggc tctactgagc agcagtgcca tctggtgcct gagatcatgt
     2581 gcttcaggca gttgtcacac tgcatctaca gccaagcatc acagaaacac ttcactcatg
     2641 tggtcaggga aggtgggact gattcacatg ttagagacag gtcagatggg aaaattaagg
     2701 cccaacttgg tgttggggaa taaccatact ctggtctttc aagtgtagta gctgatgatt
     2761 cccaagaaac tgatgaattt tctgagtcca gacaaaattt ccccaagcct gctctctctg
     2821 ttggatatac ataatagaga aagtcaaatc agccctttgg ggtttgtggt aaaaattcac
     2881 tcacccattg aagcctaagg tggctttgta ccttccagat ctctcagcaa atcccctggg
     2941 cagaaatgtc acctgcttgc catgtctttg aggggttcag gtcagcctgg agaacaggct
     3001 ccttaggtca aaaaccttgt tttaagagcc actgtggtac agtcaatgta acccacttgt
     3061 gcagaagcca tgcttccaag gaaggtctca ggttgacaac tagacatcaa atctgagaag
     3121 ccaactgtgg agtaatgtgt ttttttctct gtggacaaga gtgatcagaa aggtgcttga
     3181 tcttgaacaa ttgatccatg atgaaaaaat gaccattagc tctcccccag aagcgtctgt
     3241 gtttgctgaa tggagctgag attttgatcc gctatcagac tccagaaaga tccaggagtt
     3301 ccaagaacat ggcaaagagt caaaatctta ggacaaggaa ttaaaatgcc agaatgcaaa
     3361 tgtccaacag gcctggtagc tcacagctat caacacccta gaattccaca ctgcctcact
     3421 gcagttgctc aaggatgggt atgtttctgt ctctgaaatt cgggaaaaag ccttaagtgc
     3481 cagagaaggt gcttccaaga ctgtctgcag aattcctttc ctagggtctg tctctggtac
     3541 ccaaaagggc agagaaggaa ccctaagatt agcatagagc cactcatctc tcctcccctc
     3601 cccacccctt cttcccctgc ttccattccc catggctctc tcctctgact gtgaaacact
     3661 tcctctccag cagtgttggg aaagttctgg acctctactg gatctagcca taaatggtgg
     3721 tcttcagaaa aatggagatt gtggctgcca gcacccacaa gacagcagcc tcatgccatg
     3781 caggcagcaa gccatcctga acagcgttcc tcccagagtc tgtttcctca cctgctgttc
     3841 actggtgaga ggaaaaggga cttgttctcc cttgaccagt tttctagttt tcctgttccc
     3901 accctggtct ttgcctgatg acagagaaac tttctaaaaa aaaacaaaaa caaaagctat
     3961 aaaaaatctg caaaacaccc ta
//
