LOCUS       NM_003339               2530 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 D2 (UBE2D2),
            transcript variant 1, mRNA.
ACCESSION   NM_003339
VERSION     NM_003339.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Yan,R., Zeng,S., Gao,F., Li,L. and Xiao,X.
  TITLE     CircUBE2D2 regulates HMGB1 through miR-885-5p to promote ovarian
            cancer malignancy
  JOURNAL   Clinics (Sao Paulo) 79, 100391 (2024)
   PUBMED   38848634
  REMARK    GeneRIF: CircUBE2D2 regulates HMGB1 through miR-885-5p to promote
            ovarian cancer malignancy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Critchley,W.R., Smith,G.A., Zachary,I.C., Harrison,M.A. and
            Ponnambalam,S.
  TITLE     The E2 ubiquitin-conjugating enzymes UBE2D1 and UBE2D2 regulate
            VEGFR2 dynamics and endothelial function
  JOURNAL   J Cell Sci 136 (10) (2023)
   PUBMED   37226882
  REMARK    GeneRIF: The E2 ubiquitin-conjugating enzymes UBE2D1 and UBE2D2
            regulate VEGFR2 dynamics and endothelial function.
REFERENCE   3  (bases 1 to 2530)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2530)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 2530)
  AUTHORS   Hu,K., Liu,X., Li,Y., Li,Q., Xu,Y., Zeng,W., Zhong,G. and Yu,C.
  TITLE     Exosomes Mediated Transfer of Circ_UBE2D2 Enhances the Resistance
            of Breast Cancer to Tamoxifen by Binding to MiR-200a-3p
  JOURNAL   Med Sci Monit 26, e922253 (2020)
   PUBMED   32756532
  REMARK    GeneRIF: Exosomes Mediated Transfer of Circ_UBE2D2 Enhances the
            Resistance of Breast Cancer to Tamoxifen by Binding to MiR-200a-3p.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2530)
  AUTHORS   Anan,T., Nagata,Y., Koga,H., Honda,Y., Yabuki,N., Miyamoto,C.,
            Kuwano,A., Matsuda,I., Endo,F., Saya,H. and Nakao,M.
  TITLE     Human ubiquitin-protein ligase Nedd4: expression, subcellular
            localization and selective interaction with ubiquitin-conjugating
            enzymes
  JOURNAL   Genes Cells 3 (11), 751-763 (1998)
   PUBMED   9990509
REFERENCE   7  (bases 1 to 2530)
  AUTHORS   Hatakeyama,S., Jensen,J.P. and Weissman,A.M.
  TITLE     Subcellular localization and ubiquitin-conjugating enzyme (E2)
            interactions of mammalian HECT family ubiquitin protein ligases
  JOURNAL   J Biol Chem 272 (24), 15085-15092 (1997)
   PUBMED   9182527
REFERENCE   8  (bases 1 to 2530)
  AUTHORS   Jensen,J.P., Bates,P.W., Yang,M., Vierstra,R.D. and Weissman,A.M.
  TITLE     Identification of a family of closely related human ubiquitin
            conjugating enzymes
  JOURNAL   J Biol Chem 270 (51), 30408-30414 (1995)
   PUBMED   8530467
REFERENCE   9  (bases 1 to 2530)
  AUTHORS   Rolfe,M., Beer-Romero,P., Glass,S., Eckstein,J., Berdo,I.,
            Theodoras,A., Pagano,M. and Draetta,G.
  TITLE     Reconstitution of p53-ubiquitinylation reactions from purified
            components: the role of human ubiquitin-conjugating enzyme UBC4 and
            E6-associated protein (E6AP)
  JOURNAL   Proc Natl Acad Sci U S A 92 (8), 3264-3268 (1995)
   PUBMED   7724550
REFERENCE   10 (bases 1 to 2530)
  AUTHORS   Scheffner,M., Huibregtse,J.M. and Howley,P.M.
  TITLE     Identification of a human ubiquitin-conjugating enzyme that
            mediates the E6-AP-dependent ubiquitination of p53
  JOURNAL   Proc Natl Acad Sci U S A 91 (19), 8797-8801 (1994)
   PUBMED   8090726
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC033349.1, AK001311.1,
            AK001428.1 and AF317220.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003339.2.
            
            Summary: Regulated degradation of misfolded, damaged or short-lived
            proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome
            system (UPS). An integral part of the UPS system is the
            ubiquitination of target proteins and covalent linkage of
            Ub-containing proteins to form polymeric chains, marking them as
            targets for 26S proteasome-mediated degradation. Ubiquitination of
            proteins is mediated by a cascade of enzymes which includes E1
            (ubiquitin activating), E2 (ubiquitin conjugating), and E3
            (ubiquitin ligases) enzymes. This gene encodes a member of the E2
            enzyme family. Substrates of this enzyme include the tumor
            suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5).
            Alternative splicing results in multiple transcript variants of
            this gene. [provided by RefSeq, May 2013].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK001428.1, SRR3476690.977885.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000398733.8/ ENSP00000381717.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1005              BC033349.1         174-1178
            1006-1786           AK001311.1         952-1732
            1787-2390           AK001428.1         1787-2390
            2391-2530           AF317220.1         1860-1999
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q31.2"
     gene            1..2530
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /note="ubiquitin conjugating enzyme E2 D2"
                     /db_xref="GeneID:7322"
                     /db_xref="HGNC:HGNC:12475"
                     /db_xref="MIM:602962"
     exon            1..477
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /inference="alignment:Splign:2.1.0"
     CDS             454..897
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /EC_number="2.3.2.23"
                     /EC_number="2.3.2.24"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin-conjugating enzyme E2D 2 (homologous to yeast
                     UBC4/5); ubiquitin-conjugating enzyme E2-17 kDa 2;
                     ubiquitin conjugating enzyme E2D 2; ubiquitin-protein
                     ligase D2; ubiquitin carrier protein D2; E2
                     ubiquitin-conjugating enzyme D2; p53-regulated
                     ubiquitin-conjugating enzyme 1; (E3-independent) E2
                     ubiquitin-conjugating enzyme D2"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 D2 isoform 1"
                     /protein_id="NP_003330.1"
                     /db_xref="CCDS:CCDS43369.1"
                     /db_xref="GeneID:7322"
                     /db_xref="HGNC:HGNC:12475"
                     /db_xref="MIM:602962"
                     /translation="MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSP
                     YQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKV
                     LLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM"
     exon            478..541
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /inference="alignment:Splign:2.1.0"
     exon            542..573
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /inference="alignment:Splign:2.1.0"
     exon            574..651
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /inference="alignment:Splign:2.1.0"
     exon            652..757
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /inference="alignment:Splign:2.1.0"
     exon            758..851
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /inference="alignment:Splign:2.1.0"
     exon            852..2530
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      926..931
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /note="hexamer: AATAAA"
     polyA_site      955
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /note="major polyA site"
     regulatory      1757..1762
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /note="hexamer: AATAAA"
     polyA_site      1788
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /note="major polyA site"
     regulatory      2502..2507
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
                     /note="hexamer: AATAAA"
     polyA_site      2530
                     /gene="UBE2D2"
                     /gene_synonym="E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4;
                     UBCH5B"
ORIGIN      
        1 ggccccaccc cggcggcgca gcccgcccgc ccgcgcgtcc ctcggtccac ctgcagcagg
       61 gaggaagaca ggcaatccct ccggctgtcc gaccaagaga ggccggccga gcccgaggct
      121 tgggcttttg ctttctggcg gagggatctg cggcggttta ggaggcggcg ctgatcctgg
      181 gaggaagagg cagctacggc ggcggcggcg gtggcggcta gggcggcggc gaataaaggg
      241 gccgccgccg ggtgatgcgg tgaccgctgc ggcaggccca ggagctgagt gggccccggc
      301 cctcagcccg tcccgccgga cccgctttcc tcaactctcc atcttctcct gccgaccgag
      361 atcgccgagg cggcctcagg ctccctagcc ccttccccgt cccttccccg cccccgtccc
      421 cgccccgggg gccgccgcca cccgcctccc accatggctc tgaagagaat ccacaaggaa
      481 ttgaatgatc tggcacggga ccctccagca cagtgttcag caggtcctgt tggagatgat
      541 atgttccatt ggcaagctac aataatgggg ccaaatgaca gtccctatca gggtggagta
      601 tttttcttga caattcattt cccaacagat taccccttca aaccacctaa ggttgcattt
      661 acaacaagaa tttatcatcc aaatattaac agtaatggca gcatttgtct tgatattcta
      721 cgatcacagt ggtctccagc actaactatt tcaaaagtac tcttgtccat ctgttctctg
      781 ttgtgtgatc ccaatccaga tgatccttta gtgcctgaga ttgctcggat ctacaaaaca
      841 gatagagaaa agtacaacag aatagctcgg gaatggactc agaagtatgc gatgtaatta
      901 aagaaattat tggataacct ctacaaataa agatagggga actctgaaag agaaagtcct
      961 tttgatttcc atttgactgc tttctatgag cccacgcctc atcttcccct gtgcacatgt
     1021 ttacctgata cagcagtgct gcgtgttgta catacttgga acaacaaact agaaatactg
     1081 tacttctgta ccaacattgc ctcctagcag agaagtgtgt gtgtgacaag ccagttctac
     1141 aggcattacc taggtgtgag actaaaagct tttcttattg acttaaattt ggataacagc
     1201 aaggtgtgag gggggtggtg ggtatggtgt gtgcttggat gggaaagaaa aggctccact
     1261 cacctatagg agattatttt taagtggaat ccatttaaac tcaaaacagt tatgaaaagc
     1321 aaggtgaaga acatgaagct gtgtctgtat tcattttatt ccgaaggagc tacgtcttag
     1381 gtgaaagtta tgaccaacca gattaaactc tacccacatc ctgcatttta aggtctaagt
     1441 ttaactggtc aacatttaaa tggattggag ctattagtac atcaagtgtg atgggctttg
     1501 ttcccaactc ttttacatct ccctacccct tcaacctttg gcctttcagc ccttctttct
     1561 ctcttccata ttctttggtt tgtatgtggt ttctcagtta atacatagct aatagctctt
     1621 atttttctta tgtttttaac cgcttaggtc tatttggatg taagggtgaa aattcatttg
     1681 atggaaatac ttgtgtatat ttaaagaccc aattgctcct ctggagcttg tactttcaag
     1741 aatgattaat ctgtgtaata aactggttac tacagtcatt acatataatt ttgtgtgaat
     1801 aggctttttc atttttaaga agtttgtcta gctgagatta gtggtggatt ttctcccact
     1861 tctgaaatgt tcatttatac tggttgcatt ttaagatcat gaaacaattc cagttacatt
     1921 gtaaaaagga tatcttacga gtaattttat tgaacaagtt agaggcataa gcttaagagc
     1981 atttccatga aacaacacat gcagcattcc aggaacttga ttgttaaatt caataagaaa
     2041 tttgctttat taatgaaact aagctgcatt tcatcaaaac cttgtgacat tcccttggta
     2101 cataggacat aaaacacaga ggcattgcta tttggtaagt taagcttctg tgattgtaat
     2161 tataaaagag caacattgac caaacctggg aaacaagagc acagtcttgt ttggagagtc
     2221 tacataatta ctttgcacta acatttgcag gatgttcaca caattttaaa ttgtactgta
     2281 tgtggctttt tgaagtcttc ccttgaccct agtaaaatat agcttgaaac ttgtaaacaa
     2341 ctgtgtttgc cagaaacatc attcatgtga actaggcaag ttaccttttt tccccccttc
     2401 ttttcctaat tgtaaactag gccaacctga aagccatggc tgatgctcta gccatcaggt
     2461 tctttcaaat gcatctttac actcttgcac aaaagttaag gaataaatgt ccactgcttt
     2521 tggttttaaa
//
