LOCUS       NM_003338               2623 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 D1 (UBE2D1),
            transcript variant 1, mRNA.
ACCESSION   NM_003338
VERSION     NM_003338.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Critchley,W.R., Smith,G.A., Zachary,I.C., Harrison,M.A. and
            Ponnambalam,S.
  TITLE     The E2 ubiquitin-conjugating enzymes UBE2D1 and UBE2D2 regulate
            VEGFR2 dynamics and endothelial function
  JOURNAL   J Cell Sci 136 (10) (2023)
   PUBMED   37226882
  REMARK    GeneRIF: The E2 ubiquitin-conjugating enzymes UBE2D1 and UBE2D2
            regulate VEGFR2 dynamics and endothelial function.
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Kaur,A., Gladu,E.M., Wright,K.M., Webb,J.A. and Massiah,M.A.
  TITLE     B-box1 Domain of MID1 Interacts with the Ube2D1 E2 Enzyme
            Differently Than RING E3 Ligases
  JOURNAL   Biochemistry 62 (5), 1012-1025 (2023)
   PUBMED   36820504
  REMARK    GeneRIF: B-box1 Domain of MID1 Interacts with the Ube2D1 E2 Enzyme
            Differently Than RING E3 Ligases.
REFERENCE   3  (bases 1 to 2623)
  AUTHORS   Seo,S.U., Woo,S.M., Kim,M.W., Lee,E.W., Min,K.J. and Kwon,T.K.
  TITLE     Phosphorylation of OTUB1 at Tyr 26 stabilizes the mTORC1 component,
            Raptor
  JOURNAL   Cell Death Differ 30 (1), 82-93 (2023)
   PUBMED   35927303
REFERENCE   4  (bases 1 to 2623)
  AUTHORS   Ray,P., Nancarrow,D.J., Ferrer-Torres,D., Wang,Z., San Martinho,M.,
            Hinton,T., Wu,J.H., Wu,A., Turgeon,D.K., Hammer,M.A., Dame,M.K.,
            Lawrence,T.S., O'Brien,P.J., Spence,J.R., Beer,D.G. and Ray,D.
  TITLE     UBCH5 Family Members Differentially Impact Stabilization of Mutant
            p53 via RNF128 Iso1 During Barrett's Progression to Esophageal
            Adenocarcinoma
  JOURNAL   Cell Mol Gastroenterol Hepatol 13 (1), 129-149 (2022)
   PUBMED   34416429
  REMARK    GeneRIF: UBCH5 Family Members Differentially Impact Stabilization
            of Mutant p53 via RNF128 Iso1 During Barrett's Progression to
            Esophageal Adenocarcinoma.
REFERENCE   5  (bases 1 to 2623)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2623)
  AUTHORS   Nuber,U. and Scheffner,M.
  TITLE     Identification of determinants in E2 ubiquitin-conjugating enzymes
            required for hect E3 ubiquitin-protein ligase interaction
  JOURNAL   J Biol Chem 274 (11), 7576-7582 (1999)
   PUBMED   10066826
REFERENCE   7  (bases 1 to 2623)
  AUTHORS   Gutierrez,J.A., Yu,J. and Wessling-Resnick,M.
  TITLE     Characterization and chromosomal mapping of the human gene for SFT,
            a stimulator of Fe transport
  JOURNAL   Biochem Biophys Res Commun 253 (3), 739-742 (1998)
   PUBMED   9918797
REFERENCE   8  (bases 1 to 2623)
  AUTHORS   Hatakeyama,S., Jensen,J.P. and Weissman,A.M.
  TITLE     Subcellular localization and ubiquitin-conjugating enzyme (E2)
            interactions of mammalian HECT family ubiquitin protein ligases
  JOURNAL   J Biol Chem 272 (24), 15085-15092 (1997)
   PUBMED   9182527
REFERENCE   9  (bases 1 to 2623)
  AUTHORS   Jensen,J.P., Bates,P.W., Yang,M., Vierstra,R.D. and Weissman,A.M.
  TITLE     Identification of a family of closely related human ubiquitin
            conjugating enzymes
  JOURNAL   J Biol Chem 270 (51), 30408-30414 (1995)
   PUBMED   8530467
REFERENCE   10 (bases 1 to 2623)
  AUTHORS   Scheffner,M., Huibregtse,J.M. and Howley,P.M.
  TITLE     Identification of a human ubiquitin-conjugating enzyme that
            mediates the E6-AP-dependent ubiquitination of p53
  JOURNAL   Proc Natl Acad Sci U S A 91 (19), 8797-8801 (1994)
   PUBMED   8090726
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA677507.1, BC005980.1 and
            AC023170.10.
            
            On Nov 23, 2018 this sequence version replaced NM_003338.4.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes a member of the E2
            ubiquitin-conjugating enzyme family. This enzyme is closely related
            to a stimulator of iron transport (SFT), and is up-regulated in
            hereditary hemochromatosis. It also functions in the ubiquitination
            of the tumor-suppressor protein p53 and the hypoxia-inducible
            transcription factor HIF1alpha by interacting with the E1
            ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
            Two transcript variants encoding different isoforms have been found
            for this gene. [provided by RefSeq, Mar 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.608486.1,
                                           SRR1660809.223931.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373910.9/ ENSP00000363019.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 DA677507.1         28-29
            3-1466              BC005980.1         1-1464
            1467-2623           AC023170.10        152367-153523       c
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q21.1"
     gene            1..2623
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /note="ubiquitin conjugating enzyme E2 D1"
                     /db_xref="GeneID:7321"
                     /db_xref="HGNC:HGNC:12474"
                     /db_xref="MIM:602961"
     exon            1..220
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /inference="alignment:Splign:2.1.0"
     CDS             197..640
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /EC_number="2.3.2.23"
                     /EC_number="2.3.2.24"
                     /note="isoform 1 is encoded by transcript variant 1;
                     stimulator of Fe transport; ubiquitin-conjugating enzyme
                     E2D 1 (UBC4/5 homolog, yeast); ubiquitin-conjugating
                     enzyme E2-17 kDa 1; ubiquitin conjugating enzyme E2D 1;
                     ubiquitin-protein ligase D1; ubiquitin carrier protein D1;
                     ubiquitin-conjugating enzyme E2(17)KB 1; E2
                     ubiquitin-conjugating enzyme D1; (E3-independent) E2
                     ubiquitin-conjugating enzyme D1"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 D1 isoform 1"
                     /protein_id="NP_003329.1"
                     /db_xref="CCDS:CCDS7252.1"
                     /db_xref="GeneID:7321"
                     /db_xref="HGNC:HGNC:12474"
                     /db_xref="MIM:602961"
                     /translation="MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSA
                     YQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKV
                     LLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM"
     exon            221..284
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /inference="alignment:Splign:2.1.0"
     exon            285..316
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /inference="alignment:Splign:2.1.0"
     exon            317..394
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /inference="alignment:Splign:2.1.0"
     exon            395..500
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /inference="alignment:Splign:2.1.0"
     exon            501..594
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /inference="alignment:Splign:2.1.0"
     exon            595..2623
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1451..1456
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /note="hexamer: AATAAA"
     polyA_site      1471
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /note="major polyA site"
     regulatory      2604..2609
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /note="hexamer: ATTAAA"
     polyA_site      2623
                     /gene="UBE2D1"
                     /gene_synonym="E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A"
                     /note="major polyA site"
ORIGIN      
        1 gcacactcgc gcacactcgc gctcgggcgc acacggagca gggaccggcg cccggagcga
       61 gccagggagc ggctaaccgg ggacccaccg cgcggagcca gcctagctgc cagcgagccc
      121 aacccgcgac gacccacgcc cctgagcccc gcagccgacc cctgccggcc ggtgtcccca
      181 ccgccatccc tgacccatgg cgctgaagag gattcagaaa gaattgagtg atctacagcg
      241 cgatccacct gctcactgtt cagctggacc tgtgggagat gacttgttcc actggcaagc
      301 cactattatg gggcctcctg atagcgcata tcaaggtgga gtcttctttc tcactgtaca
      361 ttttccgaca gattatcctt ttaaaccacc aaagattgct ttcacaacaa aaatttacca
      421 tccaaacata aacagtaatg gaagtatttg tctcgatatt ctgaggtcac aatggtcacc
      481 agctctgact gtatcaaaag ttttattgtc catatgttct ctactttgtg atcctaatcc
      541 agatgacccc ttagtaccag atattgcaca aatctataaa tcagacaaag aaaaatacaa
      601 cagacatgca agagaatgga ctcagaaata tgcaatgtaa aaatcaaaaa cattttcata
      661 tataccagag tactgtaaaa tctaggtttt tttcaacatt agcagtaaat tgagcactgt
      721 ttactgtttc attgtaccat gaaaccattt gatttttacc cattttaaat gtgtttctga
      781 agcaagacaa aacaaacttc caaaaatacc cttaagactg tgatgagagc atttatcatt
      841 ttgtatgcat tgagaaagac atttattatg gtttttaaga tacttggaca tctgcatctt
      901 cagcttacaa gatctacaat gcagctgaaa agcaaccaaa ttattttttg ctgaaactag
      961 atgtttttac atgagaaata ctgtatgtgt tgtctaagat gtcagtttta taaatctgta
     1021 ttcagatttc attctttgtt agctcacttt ataatttgta tttttttact gtatagacta
     1081 aatatattct atttacatgt atgtcaactc attacttttt tcctgtgaac agtattgaaa
     1141 aaccccaacg gctgataatt aagtgaatta actgtgtctc ccttgtctta ggatattctg
     1201 tagattgatt gcagatttct taaatctgaa atgatcttta cactgtaatt ctcagcatac
     1261 tgattatgga gaaacacttg ttttgatttt gttatacttg acttaacttt attgcaatgt
     1321 gaattaattg cactgctaag taggaagatg tgtaactttt atttgttgct attcacattt
     1381 gaattttttc ctgtataggc aatattatat tgacaccttt tacagatctt actgtagctt
     1441 tttccatata aataaaatgc tttttctact atttgtcttg attacttaaa aaaataaaaa
     1501 tataagtaag gatcaaaact ctaaaatttt gcatgaaaat tacatccaaa ttgtgaaaat
     1561 cagatctatt ttgtttgcca ttagtcacca ttagttatat aaattttatt gttttaggtt
     1621 agtatctctt tactaaattg tcagtctata agataatata tgttgatccc ttgctgtaga
     1681 ggagaattta gagtaatttg gggtttgtct tggattatat ctaaatggat tatttgttaa
     1741 aagtactgaa atgagtataa ggcagtatca cccatccaaa agaaaggtct ttatagacct
     1801 gcacagtcac tagattaatt cattaaaatg cccccaccct gatgtaattg acattacatt
     1861 tcttaacatt ttaaaatcta gaatttctaa aatggaattt aatgccatca caatttgaaa
     1921 aacttttttt ttttttttac tatagaagtt acaaaggaag ttctaaaatt atgcctccct
     1981 ctgtttttat aagttgccat cgaaaagtga tttaaataag caggttatct ttatagattt
     2041 taaagaaaac tagaaagttt taatgtttta acttggggaa aaatacatct ctttaatgtt
     2101 tagcatgctt gtcaaccttg agtgagtgtc atttttaaga acagttgtag cccttctgat
     2161 tattgcagta gctgtagaag tatgtaagaa tatgtgatgg gtgtagtcat tagcaaagca
     2221 tttaaatcac ttgagtattt tgtcatggtt cattattatt aaagcacaaa ataacctatt
     2281 gttagaaaat atgtgttttt ataaatgaat gtaaaataat taaatgaatt gtgaaatgga
     2341 tgtttaagaa aatataggct taaaaagtaa atctataaaa tgatgtctta aaacagccat
     2401 atcatgaaaa attctactta gctatattat tataagctac atttgccctg aatttgaaca
     2461 ctcaacatca ctagatttaa atatttagta tattttgata gtaaagggtt ttgtttcttg
     2521 aatatcttca ctttaaacaa aaaaaaaaaa caactttcat ttgtgtggca tttatttttg
     2581 gaagtgtctt cttttttttc tttattaaag tttttgaaac ttg
//
