LOCUS       NM_003337               2241 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 B (UBE2B), mRNA.
ACCESSION   NM_003337
VERSION     NM_003337.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Barnsby-Greer,L., Mabbitt,P.D., Dery,M.A., Squair,D.R., Wood,N.T.,
            Lamoliatte,F., Lange,S.M. and Virdee,S.
  TITLE     UBE2A and UBE2B are recruited by an atypical E3 ligase module in
            UBR4
  JOURNAL   Nat Struct Mol Biol 31 (2), 351-363 (2024)
   PUBMED   38182926
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Shukla,P.K., Sinha,D., Leng,A.M., Bissell,J.E., Thatipamula,S.,
            Ganguly,R., Radmall,K.S., Skalicky,J.J., Shrieve,D.C. and
            Chandrasekharan,M.B.
  TITLE     Mutations of Rad6 E2 ubiquitin-conjugating enzymes at alanine-126
            in helix-3 affect ubiquitination activity and decrease enzyme
            stability
  JOURNAL   J Biol Chem 298 (11), 102524 (2022)
   PUBMED   36162503
  REMARK    GeneRIF: Mutations of Rad6 E2 ubiquitin-conjugating enzymes at
            alanine-126 in helix-3 affect ubiquitination activity and decrease
            enzyme stability.
REFERENCE   3  (bases 1 to 2241)
  AUTHORS   Zeng,X., Zheng,W., Sheng,Y. and Ma,H.
  TITLE     UBE2B promotes ovarian cancer growth via promoting RAD18 mediated
            ZMYM2 monoubiquitination and stabilization
  JOURNAL   Bioengineered 13 (4), 8000-8012 (2022)
   PUBMED   35313791
  REMARK    GeneRIF: UBE2B promotes ovarian cancer growth via promoting RAD18
            mediated ZMYM2 monoubiquitination and stabilization.
REFERENCE   4  (bases 1 to 2241)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2241)
  AUTHORS   Sarma,A., Gajan,A., Kim,S., Gurdziel,K., Mao,G., Nangia-Makker,P.
            and Shekhar,M.P.V.
  TITLE     RAD6B Loss Disrupts Expression of Melanoma Phenotype in Part by
            Inhibiting WNT/beta-Catenin Signaling
  JOURNAL   Am J Pathol 191 (2), 368-384 (2021)
   PUBMED   33181138
  REMARK    GeneRIF: RAD6B Loss Disrupts Expression of Melanoma Phenotype in
            Part by Inhibiting WNT/beta-Catenin Signaling.
REFERENCE   6  (bases 1 to 2241)
  AUTHORS   Koken,M.H., Hoogerbrugge,J.W., Jasper-Dekker,I., de Wit,J.,
            Willemsen,R., Roest,H.P., Grootegoed,J.A. and Hoeijmakers,J.H.
  TITLE     Expression of the ubiquitin-conjugating DNA repair enzymes HHR6A
            and B suggests a role in spermatogenesis and chromatin modification
  JOURNAL   Dev Biol 173 (1), 119-132 (1996)
   PUBMED   8575614
REFERENCE   7  (bases 1 to 2241)
  AUTHORS   Koken,M.H., Smit,E.M., Jaspers-Dekker,I., Oostra,B.A.,
            Hagemeijer,A., Bootsma,D. and Hoeijmakers,J.H.
  TITLE     Localization of two human homologs, HHR6A and HHR6B, of the yeast
            DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31
  JOURNAL   Genomics 12 (3), 447-453 (1992)
   PUBMED   1559696
REFERENCE   8  (bases 1 to 2241)
  AUTHORS   Koken,M.H., Reynolds,P., Jaspers-Dekker,I., Prakash,L., Prakash,S.,
            Bootsma,D. and Hoeijmakers,J.H.
  TITLE     Structural and functional conservation of two human homologs of the
            yeast DNA repair gene RAD6
  JOURNAL   Proc Natl Acad Sci U S A 88 (20), 8865-8869 (1991)
   PUBMED   1717990
REFERENCE   9  (bases 1 to 2241)
  AUTHORS   Woffendin,C., Chen,Z.Y., Staskus,K., Retzel,E.F. and Plagemann,P.G.
  TITLE     Mammalian mRNAs encoding protein closely related to
            ubiquitin-conjugating enzyme encoded by yeast DNA repair gene RAD6
  JOURNAL   Biochim Biophys Acta 1090 (1), 81-85 (1991)
   PUBMED   1883845
REFERENCE   10 (bases 1 to 2241)
  AUTHORS   Schneider,R., Eckerskorn,C., Lottspeich,F. and Schweiger,M.
  TITLE     The human ubiquitin carrier protein E2(Mr = 17,000) is homologous
            to the yeast DNA repair gene RAD6
  JOURNAL   EMBO J 9 (5), 1431-1435 (1990)
   PUBMED   2158443
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK312012.1, M74525.1,
            BU954901.1 and T88933.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_003337.3.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes a member of the E2
            ubiquitin-conjugating enzyme family. This enzyme is required for
            post-replicative DNA damage repair. Its protein sequence is 100%
            identical to the mouse, rat, and rabbit homologs, which indicates
            that this enzyme is highly conserved in eukaryotic evolution.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.241935.1,
                                           SRR1803613.64513.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265339.7/ ENSP00000265339.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-486               AK312012.1         58-543
            487-1322            M74525.1           881-1716
            1323-1668           BU954901.1         64-409
            1669-2124           M74525.1           2062-2517
            2125-2241           T88933.1           1-117               c
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q31.1"
     gene            1..2241
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /note="ubiquitin conjugating enzyme E2 B"
                     /db_xref="GeneID:7320"
                     /db_xref="HGNC:HGNC:12473"
                     /db_xref="MIM:179095"
     exon            1..71
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..486
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /EC_number="2.3.2.23"
                     /note="ubiquitin-conjugating enzyme E2B (RAD6 homolog); E2
                     protein; ubiquitin-protein ligase B; ubiquitin conjugating
                     enzyme E2B; ubiquitin carrier protein B; RAD6 homolog B;
                     ubiquitin-conjugating enzyme E2-17 kDa; E2
                     ubiquitin-conjugating enzyme B"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 B"
                     /protein_id="NP_003328.1"
                     /db_xref="CCDS:CCDS4174.1"
                     /db_xref="GeneID:7320"
                     /db_xref="HGNC:HGNC:12473"
                     /db_xref="MIM:179095"
                     /translation="MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPE
                     GTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDV
                     SSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS"
     exon            72..152
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            153..178
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            179..268
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            269..357
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /inference="alignment:Splign:2.1.0"
     exon            358..2241
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      893..898
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /note="hexamer: AATACA"
     polyA_site      912
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /note="major polyA site"
     regulatory      1647..1652
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /note="hexamer: AGTAAA"
     polyA_site      1667
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
     regulatory      1731..1736
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /note="hexamer: CATAAA"
     polyA_site      1750
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
     regulatory      2103..2108
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
                     /note="hexamer: AATAAA"
     polyA_site      2125
                     /gene="UBE2B"
                     /gene_synonym="E2-17kDa; HHR6B; HR6B; RAD6B; UBC2"
ORIGIN      
        1 agactgaccg cggggcagct gcggagcatg tcgaccccgg cccggaggag gctcatgcgg
       61 gatttcaagc ggttacaaga ggacccacct gtgggtgtca gtggcgcacc atctgaaaac
      121 aacatcatgc agtggaatgc agttatattt ggaccagaag ggacaccttt tgaagatggt
      181 acttttaaac tagtaataga attttctgaa gaatatccaa ataaaccacc aactgttagg
      241 tttttatcca aaatgtttca tccaaatgtg tatgctgatg gtagcatatg tttagatatc
      301 cttcagaatc gatggagtcc aacatatgat gtatcttcta tcttaacatc aattcagtct
      361 ctgctggatg aaccgaatcc taacagtcca gccaatagcc aggcagcaca gctttatcag
      421 gaaaacaaac gagaatatga gaaaagagtt tcggccattg ttgaacaaag ctggaatgat
      481 tcataataga caactggtct gttaatcttt ttcatcattg ttgtgtataa tttacctctc
      541 attagaaagg ctaacaaatt ttaagtgcca caggttttaa ggattctgca gaaaaaaaag
      601 aaaaaagtcc ttcagtttag aacctacaaa agcttgtgta tcttgattaa tgtacttttt
      661 attgcatggt gtgaactaag ttattgctgc ataaatttgt aatatatcct gtttgtattt
      721 ttttccaagt gtataatgtt ggtgtggagt tttcatgaca gaatatacac attttgtaaa
      781 tctgtacttt tttcaaatat tgaatgcctt atttttgaat tctttagatt tttaaattgg
      841 agaaaagcac ttaaagtttt ttatatatga atattacatg taaagctgtt aaaatacata
      901 acttcagtgc aagagacttt gtcacttatt tccttatgtg tgtaggaggg gttaataagt
      961 ctctagctct ccatctattg atagtttcat ttacaatttc aaaagaacat tcttatattt
     1021 tatcaaggaa gtcttcaaat ttgattctaa atagcgatta taatctccaa ctttattttg
     1081 aatgtacctc tattagtttc aattgagtaa ttctagacat aactggtttg actctgtcca
     1141 actctgtatt taggccattt gttacagttt cttcatgcat tacttactgt taaaactgta
     1201 ccttttgcga tttcacagtt ggcacttctg ccatgagcag agaactgatg cgacttgttt
     1261 tgctgcttgg tagcacttta aaaaattttt tgattaatga agaaagtaaa accataaaca
     1321 tttgccaaaa attcatgccc cagtattagc aatgaattag ttgcattggt ttgagaaagg
     1381 cacatattgg agggaaatct tggtgtaact taaatatttg aaaattacct ttaatgcaat
     1441 gcatatctgt ttattctggg aaatgtttta atgccagggc ctgctgagtt gcttcttctt
     1501 gtggagattt tttttttaat ctcctgagtt gtataaaagt tgtactgcat cttagtttac
     1561 tggataaatt taaaacacag tattgtagaa agctaataca aaactatcct atgccttcaa
     1621 atagtataga aaatggaaaa tatacaagta aattctgttg aaccacctgt gtagtctctc
     1681 tagtagttaa aacagctatt ttagtaaccc caagagtttc ttctcatatc cataaaattg
     1741 gattacagta tggcaatatc tacagcttct attcattatg caacgtttaa cattgattgg
     1801 ataaatgctt ataggctggg ccaggtggct cccacctgta gtcccagcac tttgggaggc
     1861 aaaggcggga ggatcacttg aggccaggac tttgagacca gccagggcaa cataataaga
     1921 cttttctcta ctttaaattt tttaaaaaat tagttgggca tagtggcact tgcctgtagt
     1981 ctcagctact taggaggctg aggtggcaga atagcttatg cctaggaggt tgaggctgca
     2041 gtgagctgtg attatgccat tgcactccag cctgggtgac agagcaatac cctgtcttta
     2101 aaaataaaag ctattacttg gcaaattctg aggtaagtgt atgatcttag ggaactttga
     2161 tacaaatggg aattcactca ctggaatcgt ggctcccaaa aagatgcttt aattgtgtgg
     2221 tgcttttata gtttagctcc a
//
