LOCUS       NM_003968               2111 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3),
            transcript variant 1, mRNA.
ACCESSION   NM_003968
VERSION     NM_003968.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Zhang,H., Yang,J., Song,Q., Ding,X., Sun,F. and Yang,L.
  TITLE     UBA3 promotes the occurrence and metastasis of intrahepatic
            cholangiocarcinoma through MAPK signaling pathway
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 56 (2), 199-209 (2024)
   PUBMED   38298057
  REMARK    GeneRIF: UBA3 promotes the occurrence and metastasis of
            intrahepatic cholangiocarcinoma through MAPK signaling pathway.
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Liu,H., Shih,Y.H., Wang,W.L., Chang,W.L. and Wang,Y.C.
  TITLE     UBE1C is upregulated and promotes neddylation of p53 in lung cancer
  JOURNAL   FASEB J 37 (10), e23181 (2023)
   PUBMED   37668436
  REMARK    GeneRIF: UBE1C is upregulated and promotes neddylation of p53 in
            lung cancer.
REFERENCE   3  (bases 1 to 2111)
  AUTHORS   Lin,X., Yang,S., Zhou,C., Ao,C. and Sun,D.
  TITLE     The NEDD8-activating enzyme E1 UBA3 orchestrates the
            immunosuppressive microenvironment in lung adenocarcinoma via the
            NF-small ka, CyrillicB pathway
  JOURNAL   Med Oncol 40 (10), 286 (2023)
   PUBMED   37656220
  REMARK    GeneRIF: The NEDD8-activating enzyme E1 UBA3 orchestrates the
            immunosuppressive microenvironment in lung adenocarcinoma via the
            NF-small ka, CyrillicB pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2111)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 2111)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2111)
  AUTHORS   Bohnsack,R.N. and Haas,A.L.
  TITLE     Conservation in the mechanism of Nedd8 activation by the human
            AppBp1-Uba3 heterodimer
  JOURNAL   J Biol Chem 278 (29), 26823-26830 (2003)
   PUBMED   12740388
  REMARK    GeneRIF: Conservation in the mechanism of Nedd8 activation by the
            human AppBp1-Uba3 heterodimer.
REFERENCE   7  (bases 1 to 2111)
  AUTHORS   Walden,H., Podgorski,M.S. and Schulman,B.A.
  TITLE     Insights into the ubiquitin transfer cascade from the structure of
            the activating enzyme for NEDD8
  JOURNAL   Nature 422 (6929), 330-334 (2003)
   PUBMED   12646924
  REMARK    GeneRIF: structure and mutational analysis of human APPBP1-UBA3,
            the heterodimeric E1 enzyme for NEDD8
REFERENCE   8  (bases 1 to 2111)
  AUTHORS   Chen,Y., McPhie,D.L., Hirschberg,J. and Neve,R.L.
  TITLE     The amyloid precursor protein-binding protein APP-BP1 drives the
            cell cycle through the S-M checkpoint and causes apoptosis in
            neurons
  JOURNAL   J Biol Chem 275 (12), 8929-8935 (2000)
   PUBMED   10722740
REFERENCE   9  (bases 1 to 2111)
  AUTHORS   Gong,L. and Yeh,E.T.
  TITLE     Identification of the activating and conjugating enzymes of the
            NEDD8 conjugation pathway
  JOURNAL   J Biol Chem 274 (17), 12036-12042 (1999)
   PUBMED   10207026
REFERENCE   10 (bases 1 to 2111)
  AUTHORS   Osaka,F., Kawasaki,H., Aida,N., Saeki,M., Chiba,T., Kawashima,S.,
            Tanaka,K. and Kato,S.
  TITLE     A new NEDD8-ligating system for cullin-4A
  JOURNAL   Genes Dev 12 (15), 2263-2268 (1998)
   PUBMED   9694792
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CD700642.1, BC022853.1,
            AK002159.1 and AL117566.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003968.3.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes a member of the E1
            ubiquitin-activating enzyme family. The encoded enzyme associates
            with AppBp1, an amyloid beta precursor protein binding protein, to
            form a heterodimer, and then the enzyme complex activates NEDD8, a
            ubiquitin-like protein, which regulates cell division, signaling
            and embryogenesis. Multiple alternatively spliced transcript
            variants encoding distinct isoforms have been found for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.773118.1,
                                           SRR3476690.904367.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361055.9/ ENSP00000354340.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                CD700642.1         39-76
            39-1515             BC022853.1         46-1522
            1516-1792           BC022853.1         1524-1800
            1793-2110           AK002159.1         1746-2063
            2111-2111           AL117566.1         2113-2113
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p14.1"
     gene            1..2111
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /note="ubiquitin like modifier activating enzyme 3"
                     /db_xref="GeneID:9039"
                     /db_xref="HGNC:HGNC:12470"
                     /db_xref="MIM:603172"
     exon            1..32
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..1404
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /EC_number="6.2.1.64"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin-activating enzyme E1C (homologous to yeast
                     UBA3); NEDD8-activating enzyme E1 subunit 2; UBA3,
                     ubiquitin-activating enzyme E1 homolog;
                     ubiquitin-activating enzyme E1C (UBA3 homolog, yeast);
                     Nedd8-activating enzyme hUba3; NEDD8-activating enzyme E1
                     catalytic subunit; ubiquitin-activating enzyme 3;
                     NEDD8-activating enzyme E1C"
                     /codon_start=1
                     /product="NEDD8-activating enzyme E1 catalytic subunit
                     isoform 1"
                     /protein_id="NP_003959.3"
                     /db_xref="CCDS:CCDS2909.1"
                     /db_xref="GeneID:9039"
                     /db_xref="HGNC:HGNC:12470"
                     /db_xref="MIM:603172"
                     /translation="MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKF
                     LERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMD
                     TIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFH
                     IIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMT
                     ACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPE
                     HIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATS
                     AYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNS
                     ASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVT
                     TPQTVLFKLHFTS"
     exon            33..74
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            75..195
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            196..276
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            277..359
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            360..440
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            441..484
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            485..549
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            550..705
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            706..808
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            809..922
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            923..976
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1013
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1095
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1196
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1260
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1315
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..2111
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2091..2096
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /note="hexamer: AATAAA"
     polyA_site      2111
                     /gene="UBA3"
                     /gene_synonym="hUBA3; NAE2; UBE1C"
                     /note="major polyA site"
ORIGIN      
        1 gcggagaaca atatggcgga tggcgaggag ccggagaaga aaagaaggag aatagaggag
       61 ctgctggctg agaaaatggc tgttgatggt gggtgtgggg acactggaga ctgggaaggt
      121 cgctggaacc atgtaaagaa gttcctcgag cgatctggac ccttcacaca ccctgatttc
      181 gaaccgagca ctgaatctct ccagttcttg ttagatacat gtaaagttct agtcattgga
      241 gctggcggct taggatgtga gctcctgaaa aatctggcct tgtctggttt tagacagatt
      301 catgttatag atatggacac tatagatgtt tccaatctaa ataggcagtt tttatttagg
      361 cctaaagata ttggaagacc taaggctgaa gttgctgcag aatttctaaa tgacagagtt
      421 cctaattgca atgtagttcc acatttcaac aagattcaag attttaacga cactttctat
      481 cgacaatttc atattattgt atgtggactg gactctatca tcgccagaag atggataaat
      541 ggcatgctga tatctcttct aaattatgaa gatggtgtct tagatccaag ctccattgtc
      601 cctttgatag atggggggac agaaggtttt aaaggaaatg cccgggtgat tctgcctgga
      661 atgactgctt gtatcgaatg cacgctggaa ctttatccac cacaggttaa ttttcccatg
      721 tgcaccattg catctatgcc caggctacca gaacactgta ttgagtatgt aaggatgttg
      781 cagtggccta aggagcagcc ttttggagaa ggggttccat tagatggaga tgatcctgaa
      841 catatacaat ggattttcca aaaatcccta gagagagcat cacaatataa tattaggggt
      901 gttacgtata ggctcactca aggggtagta aaaagaatca ttcctgcagt agcttccaca
      961 aatgcagtca ttgcagctgt gtgtgccact gaggttttta aaatagccac aagtgcatac
     1021 attcccttga ataattactt ggtgtttaat gatgtagatg ggctgtatac atacacattt
     1081 gaagcagaaa gaaaggaaaa ctgcccagct tgtagccagc ttcctcaaaa tattcagttt
     1141 tctccatcag ctaaactaca ggaggttttg gattatctaa ccaatagtgc ttctctgcaa
     1201 atgaaatctc cagccatcac agccacccta gagggaaaaa atagaacact ttacttacag
     1261 tcggtaacct ctattgaaga acgaacaagg ccaaatctct ccaaaacatt gaaagaattg
     1321 gggcttgttg atggacaaga actggcggtt gctgatgtca ccaccccaca gactgtacta
     1381 ttcaaacttc attttacttc ttaaggaaaa tctccacata atagaaaact catggaaata
     1441 atatactttg tggatgctaa gaagttgaat cgatgtcatt tttagcaata gtgttgccac
     1501 gatttgtctt tttttatata atgaaccact cttttttaac tttgtaacct tcccttgaag
     1561 acagaatttt ggtgttggtg cttgtaagca ttttcattaa taatatgaga aatgatacct
     1621 ggagagagag attatgagca aatgtattgc ttcttttaga ggaggaagca tacaacctct
     1681 tttgtgtgaa ttttgttatt atggtcaaag aatgcattcc taagttttca tttgagtacc
     1741 caaatacaca aaaggtgtcc ctttaaggaa aataaagaat taagttttaa ataacattac
     1801 attttacaat ctgacatctg gagtatattg aacataggct atttcttgat ataacactca
     1861 tttaattgtg gccatccaaa tgaatattat tgcagaattt atcttgttca taatgatttg
     1921 taaatggtgt tatagctgaa tacctgtgca tgaaaatggg caatattttc atctgtttac
     1981 ttgtagtgcc atagaggcca atatgcacaa tattaactaa tgccaagaca tggctgttta
     2041 aaaaatttaa tgttcaaaca gttatcactg atgcttttgc actatttatt aataaaatca
     2101 tatattgtgt a
//
