LOCUS       NM_005499               4005 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens ubiquitin like modifier activating enzyme 2 (UBA2),
            transcript variant 1, mRNA.
ACCESSION   NM_005499 XR_109486 XR_111852 XR_115269
VERSION     NM_005499.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Chen,H., Li,H., He,M., Lai,Z., Huang,L., Wen,D., Shi,M. and Kan,A.
  TITLE     UBA2 SUMOylates NQO1 and promotes the proliferation of
            hepatocellular carcinoma by modulating the MAPK pathway
  JOURNAL   Cancer Sci 115 (9), 2998-3012 (2024)
   PUBMED   39013843
  REMARK    GeneRIF: UBA2 SUMOylates NQO1 and promotes the proliferation of
            hepatocellular carcinoma by modulating the MAPK pathway.
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Ou,Y., Luo,H., Zhang,Q., Du,T., Liu,R., Wang,D., Chen,J., Dong,M.,
            Wang,Y., Yang,Z. and Wang,X.
  TITLE     UBA2 as a Prognostic Biomarker and Potential Therapeutic Target in
            Glioma
  JOURNAL   Front Biosci (Landmark Ed) 29 (4), 144 (2024)
   PUBMED   38682183
  REMARK    GeneRIF: UBA2 as a Prognostic Biomarker and Potential Therapeutic
            Target in Glioma.
REFERENCE   3  (bases 1 to 4005)
  AUTHORS   Du,X. and Shi,J.
  TITLE     UBA2 promotes the progression of renal cell carcinoma by
            suppressing the p53 signaling
  JOURNAL   Ir J Med Sci 191 (4), 1555-1560 (2022)
   PUBMED   34467471
  REMARK    GeneRIF: UBA2 promotes the progression of renal cell carcinoma by
            suppressing the p53 signaling.
REFERENCE   4  (bases 1 to 4005)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 4005)
  AUTHORS   Elsner,J., Mensah,M.A., Holtgrewe,M., Hertzberg,J., Bigoni,S.,
            Busche,A., Coutelier,M., de Silva,D.C., Elcioglu,N., Filges,I.,
            Gerkes,E., Girisha,K.M., Graul-Neumann,L., Jamsheer,A., Krawitz,P.,
            Kurth,I., Markus,S., Megarbane,A., Reis,A., Reuter,M.S.,
            Svoboda,D., Teller,C., Tuysuz,B., Turkmen,S., Wilson,M.,
            Woitschach,R., Vater,I., Caliebe,A., Hulsemann,W., Horn,D.,
            Mundlos,S. and Spielmann,M.
  TITLE     Genome sequencing in families with congenital limb malformations
  JOURNAL   Hum Genet 140 (8), 1229-1239 (2021)
   PUBMED   34159400
  REMARK    GeneRIF: Genome sequencing in families with congenital limb
            malformations.
REFERENCE   6  (bases 1 to 4005)
  AUTHORS   Tatham,M.H., Jaffray,E., Vaughan,O.A., Desterro,J.M., Botting,C.H.,
            Naismith,J.H. and Hay,R.T.
  TITLE     Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein
            substrates by SAE1/SAE2 and Ubc9
  JOURNAL   J Biol Chem 276 (38), 35368-35374 (2001)
   PUBMED   11451954
REFERENCE   7  (bases 1 to 4005)
  AUTHORS   Desterro,J.M., Rodriguez,M.S., Kemp,G.D. and Hay,R.T.
  TITLE     Identification of the enzyme required for activation of the small
            ubiquitin-like protein SUMO-1
  JOURNAL   J Biol Chem 274 (15), 10618-10624 (1999)
   PUBMED   10187858
REFERENCE   8  (bases 1 to 4005)
  AUTHORS   Gong,L., Li,B., Millas,S. and Yeh,E.T.
  TITLE     Molecular cloning and characterization of human AOS1 and UBA2,
            components of the sentrin-activating enzyme complex
  JOURNAL   FEBS Lett 448 (1), 185-189 (1999)
   PUBMED   10217437
REFERENCE   9  (bases 1 to 4005)
  AUTHORS   Okuma,T., Honda,R., Ichikawa,G., Tsumagari,N. and Yasuda,H.
  TITLE     In vitro SUMO-1 modification requires two enzymatic steps, E1 and
            E2
  JOURNAL   Biochem Biophys Res Commun 254 (3), 693-698 (1999)
   PUBMED   9920803
REFERENCE   10 (bases 1 to 4005)
  AUTHORS   Ueki,N., Oda,T., Kondo,M., Yano,K., Noguchi,T. and Muramatsu,M.
  TITLE     Selection system for genes encoding nuclear-targeted proteins
  JOURNAL   Nat Biotechnol 16 (13), 1338-1342 (1998)
   PUBMED   9853615
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BG503066.1, U35832.1, BM556031.1, BG678201.1, AF090384.1 and
            AC008747.5.
            
            On Nov 23, 2018 this sequence version replaced NM_005499.2.
            
            Summary: Posttranslational modification of proteins by the addition
            of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation,
            regulates protein structure and intracellular localization. SAE1
            (MIM 613294) and UBA2 form a heterodimer that functions as a
            SUMO-activating enzyme for the sumoylation of proteins (Okuma et
            al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK023120.1, SRR3476690.51052.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000246548.9/ ENSP00000246548.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-595               BG503066.1         21-615
            596-1827            U35832.1           569-1800
            1828-2206           BM556031.1         316-694
            2207-2324           BG678201.1         632-749
            2325-2647           AF090384.1         2309-2631
            2648-4005           AC008747.5         68345-69702
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.11"
     gene            1..4005
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /note="ubiquitin like modifier activating enzyme 2"
                     /db_xref="GeneID:10054"
                     /db_xref="HGNC:HGNC:30661"
                     /db_xref="MIM:613295"
     exon            1..190
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..1975
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     SUMO-1 activating enzyme subunit 2; SUMO1 activating
                     enzyme subunit 2; UBA2, ubiquitin-activating enzyme E1
                     homolog; SUMO-activating enzyme subunit 2; ubiquitin-like
                     1-activating enzyme E1B; anthracycline-associated
                     resistance ARX"
                     /codon_start=1
                     /product="SUMO-activating enzyme subunit 2 isoform 1"
                     /protein_id="NP_005490.1"
                     /db_xref="CCDS:CCDS12439.1"
                     /db_xref="GeneID:10054"
                     /db_xref="HGNC:HGNC:30661"
                     /db_xref="MIM:613295"
                     /translation="MALSRGLPRELAEAVAGGRVLVVGAGGIGCELLKNLVLTGFSHI
                     DLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFYPKANIVAYHDSIMNPDYN
                     VEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYE
                     CHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWE
                     PTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKR
                     KPPVPLDWAEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLA
                     EKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNA
                     VIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNPNCYVCASKPEV
                     TVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILISSEEGETEANNHKKLSE
                     FGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSI
                     TNGSDDGAQPSTSTAQEQDDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKR
                     SRIEQKEELDDVIALD"
     exon            191..274
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            275..345
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            346..410
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            411..511
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            512..633
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            634..701
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            702..823
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            824..923
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1090
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1184
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1297
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1453
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1550
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1656
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1793
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..4005
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2620..2625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /note="hexamer: AATAAA"
     polyA_site      2645
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /note="major polyA site"
     regulatory      3982..3987
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
                     /note="hexamer: ACTAAA"
     polyA_site      4005
                     /gene="UBA2"
                     /gene_synonym="ACCES; ARX; HRIHFB2115; SAE2"
ORIGIN      
        1 gcggctcggt tctcccgcct ccgcctccgc cgcggctcgt ggttgtcccg ccatggcact
       61 gtcgcggggg ctgccccggg agctggctga ggcggtggcc gggggccggg tgctggtggt
      121 gggggcgggc ggcatcggct gcgagctcct caagaatctc gtgctcaccg gtttctccca
      181 catcgacctg attgatctgg atactattga tgtaagcaac ctcaacagac agtttttgtt
      241 tcaaaagaaa catgttggaa gatcaaaggc acaggttgcc aaggaaagtg tactgcagtt
      301 ttacccgaaa gctaatatcg ttgcctacca tgacagcatc atgaaccctg actataatgt
      361 ggaatttttc cgacagttta tactggttat gaatgcttta gataacagag ctgcccgaaa
      421 ccatgttaat agaatgtgcc tggcagctga tgttcctctt attgaaagtg gaacagctgg
      481 gtatcttgga caagtaacta ctatcaaaaa gggtgtgacc gagtgttatg agtgtcatcc
      541 taagccgacc cagagaacct ttcctggctg tacaattcgt aacacacctt cagaacctat
      601 acattgcatc gtttgggcaa agtacttgtt caaccagttg tttggggaag aagatgctga
      661 tcaagaagta tctcctgaca gagctgaccc tgaagctgcc tgggaaccaa cggaagccga
      721 agccagagct agagcatcta atgaagatgg tgacattaaa cgtatttcta ctaaggaatg
      781 ggctaaatca actggatatg atccagttaa actttttacc aagcttttta aagatgacat
      841 caggtatctg ttgacaatgg acaaactatg gcggaaaagg aaacctccag ttccgttgga
      901 ctgggctgaa gtacaaagtc aaggagaaga aacgaatgca tcagatcaac agaatgaacc
      961 ccagttaggc ctgaaagacc agcaggttct agatgtaaag agctatgcac gtcttttttc
     1021 aaagagcatc gagactttga gagttcattt agcagaaaag ggggatggag ctgagctcat
     1081 atgggataag gatgacccat ctgcaatgga ttttgtcacc tctgctgcaa acctcaggat
     1141 gcatattttc agtatgaata tgaagagtag atttgatatc aaatcaatgg cagggaacat
     1201 tattcctgct attgctacta ctaatgcagt aattgctggg ttgatagtat tggaaggatt
     1261 gaagatttta tcaggaaaaa tagaccagtg cagaacaatt tttttgaata aacaaccaaa
     1321 cccaagaaag aagcttcttg tgccttgtgc actggatcct cccaacccca attgttatgt
     1381 atgtgccagc aagccagagg tgactgtgcg gctgaatgtc cataaagtga ctgttctcac
     1441 cttacaagac aagatagtga aagaaaaatt tgctatggta gcaccagatg tccaaattga
     1501 agatgggaaa ggaacaatcc taatatcttc cgaagaggga gagacggaag ctaataatca
     1561 caagaagttg tcagaatttg gaattagaaa tggcagccgg cttcaagcag atgacttcct
     1621 ccaggactat actttattga tcaacatcct tcatagtgaa gacctaggaa aggacgttga
     1681 atttgaagtt gttggtgatg ccccggaaaa agtggggccc aaacaagctg aagatgctgc
     1741 caaaagcata accaatggca gtgatgatgg agctcagccc tccacctcca cagctcaaga
     1801 gcaagatgac gttctcatag ttgattcaga tgaagaagat tcttcaaata atgccgacgt
     1861 cagtgaagaa gagagaagcc gcaagaggaa attagatgag aaagagaatc tcagtgcaaa
     1921 gaggtcacgt atagaacaga aggaagagct tgatgatgtc atagcattag attgaacaga
     1981 aatgcctcta aacagaaccc tcttactatt tagtttatct gggcagaacc agattgttat
     2041 gtcctttgtt ccaaagggaa aaaattgaca gcagtgactt gaaaatgatt ctgctccctt
     2101 tgaaagcatt cattttgcta gaactgttag acacattgca gtatgctgta ttgaaagtag
     2161 gaatatagtt ttaaaaaccc tttgaacaaa gtgtgtgcat aaccagtcat gagataaaac
     2221 aacacaatgc atgttgcctt tttaatgtaa atacccgtag gtatcattaa tagtttcaaa
     2281 atattgtggt ttagtaaagt tgatacctgg ttataaatat tatgccttta tttttggcta
     2341 gaagaagaat tatttttagc ctagatctaa ccattttcat actcttaact gattgaaaca
     2401 gattcaaaga agtatcgagt gctatgcatt gaaacttgtt tttaaatgtt agatggcact
     2461 atgtatatta atgtaaaaca atgttaattt actcaagttt tcagtttgta ccgcctggta
     2521 tgtctgtgta agaagccaat ttttgtgtat tgttacagtt tcaggttatt tatattcgat
     2581 gttttgtaaa actcaaataa cgactatact tatggaccaa ataaatggca tctgcattct
     2641 tgttacacat gcctgcacag ttcctgtttc tgctgcctta tatctactgc aggaatgtca
     2701 aattcttttc attaaaaaaa gaactcggtc gggcacggtg gctcatgcct gtaatcccag
     2761 cactttggaa ggctgaggca ggtggatcac ctgaggtcag tagttcaaga ccaacctggt
     2821 caacatggtg aaaccccatc tctactaaaa aaaaaaaaaa aaattagccg ggcctggtgg
     2881 caggcacctg taattccagc tactcaggag gctaaggcag cagaattgct tgaacccggg
     2941 aggcagaggt tgcagtgagc caagattgtg ctattgctac tccagccagg gcgatgagtg
     3001 aaactccatc tcaaaaaagg ttcagaagat ctccactgtt tgccaaagac gagtactttc
     3061 ttttctttat tcttggagac ttcacatgta ggaagagtac tttctaaaat aaagctcttc
     3121 ctgcgtgtgg tggtggtgca catctgtgtt ccagctactc aggaggctga ggcaggagga
     3181 tggctggagc ccagaagttc aaggccagcc tgggtaacat agcaagaccc tgtctctaaa
     3241 aaataagtaa ataaaataaa gcttttaatg gcagtgggtt ttttgttcgt ttttgagatg
     3301 aagcctcaca ctgtcatctg gcctggagtg cagtggcgcg atcctggctc actgcagcct
     3361 ccgccttcca ggtgcaagcg attctcctgt ctcagcctcc cctgtagctg ggattacagg
     3421 cgccctccac cacgctcagc taattttttg tatttttagt agagatgggg tttcactatg
     3481 ttggccaggc tggtctggaa ctcctgacct cgtgatccac ctgcctcagc ctcccaaagt
     3541 gctgggatta caggcgtgag ccactgcgcc tggcctaatg gcagtgtttt taaaatttgg
     3601 attgtcagca gttggccttt gttgagaaaa tgtgtgactt tgcccaagcc cagtaacttg
     3661 gagccttgaa tttgagatgc tggaaaggga gtccttcctc ctttctgcag tgtttgtctg
     3721 ggttgtcccc tagtttacca aagtccattt tgaatgtacc atccccagcc caactccagc
     3781 ctacagatag tgccagaccg ccagtaggtg gagtagcact gtcttcctgg tcccggacct
     3841 cagagggctt tgcctgttag ggtactctta tcactcagag atgggccttg agttttcaga
     3901 gaacagaaag ccattaagct gtttccccta ttgtattcat gtgccctttg tgtttctggc
     3961 aataatttat tttatatttc aactaaattt taaatttcta attca
//
