LOCUS       NM_003334               3572 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1),
            transcript variant 1, mRNA.
ACCESSION   NM_003334
VERSION     NM_003334.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Sirenko,M., Bernard,E., Creignou,M., Domenico,D., Farina,A., Arango
            Ossa,J.E., Kosmider,O., Hasserjian,R., Jadersten,M., Germing,U.,
            Sanz,G., van de Loosdrecht,A.A., Gurnari,C., Follo,M.Y., Thol,F.,
            Zamora,L., Pinheiro,R.F., Pellagatti,A., Elias,H.K., Haase,D.,
            Sander,B., Orna,E., Zoldan,K., Eder,L.N., Sperr,W.R.,
            Thalhammer,R., Ganster,C., Ades,L., Tobiasson,M., Palomo,L., Della
            Porta,M.G., Huberman,K., Fenaux,P., Belickova,M., Savona,M.R.,
            Klimek,V.M., Santos,F.P.S., Boultwood,J., Kotsianidis,I.,
            Santini,V., Sole,F., Platzbecker,U., Heuser,M., Valent,P.,
            Finelli,C., Voso,M.T., Shih,L.Y., Ogawa,S., Fontenay,M.,
            Jansen,J.H., Cervera,J., Ebert,B.L., Bejar,R., Greenberg,P.L.,
            Gattermann,N., Malcovati,L., Cazzola,M., Beck,D.B.,
            Hellstrom-Lindberg,E. and Papaemmanuil,E.
  TITLE     Molecular and clinical presentation of UBA1-mutated myelodysplastic
            syndromes
  JOURNAL   Blood 144 (11), 1221-1229 (2024)
   PUBMED   38687605
  REMARK    GeneRIF: Molecular and clinical presentation of UBA1-mutated
            myelodysplastic syndromes.
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Chen,C., Li,Y., Chen,Z., Shi,P., Li,Y. and Qian,S.
  TITLE     A comprehensive prognostic and immune infiltration analysis of UBA1
            in pan-cancer: A computational analysis and in vitro experiments
  JOURNAL   J Cell Mol Med 28 (16), e70037 (2024)
   PUBMED   39183260
  REMARK    GeneRIF: A comprehensive prognostic and immune infiltration
            analysis of UBA1 in pan-cancer: A computational analysis and in
            vitro experiments.
REFERENCE   3  (bases 1 to 3572)
  AUTHORS   Corty,R.W., Brogan,J., Byram,K., Springer,J., Grayson,P.C. and
            Bick,A.G.
  TITLE     VEXAS-Defining UBA1 Somatic Variants in 245,368 Diverse Individuals
            in the NIH All Of Us Cohort
  JOURNAL   Arthritis Rheumatol 76 (6), 942-948 (2024)
   PUBMED   38225170
  REMARK    GeneRIF: VEXAS-Defining UBA1 Somatic Variants in 245,368 Diverse
            Individuals in the NIH All Of Us Cohort.
REFERENCE   4  (bases 1 to 3572)
  AUTHORS   Collins,J.C., Magaziner,S.J., English,M., Hassan,B., Chen,X.,
            Balanda,N., Anderson,M., Lam,A., Fernandez-Pol,S., Kwong,B.,
            Greenberg,P.L., Terrier,B., Likhite,M.E., Kosmider,O., Wang,Y.,
            Samara,N.L., Walters,K.J., Beck,D.B. and Werner,A.
  TITLE     Shared and distinct mechanisms of UBA1 inactivation across
            different diseases
  JOURNAL   EMBO J 43 (10), 1919-1946 (2024)
   PUBMED   38360993
  REMARK    GeneRIF: Shared and distinct mechanisms of UBA1 inactivation across
            different diseases.
REFERENCE   5  (bases 1 to 3572)
  AUTHORS   Duan,S., Luo,H., Wang,Y., Jiang,D., Liu,J., Li,J., Zheng,H.,
            Zhao,T., Liu,C., Zhang,H., Mao,C., Zhang,L. and Xu,Y.
  TITLE     Dynamic monitoring of UBA1 somatic mutations in patients with
            relapsing polychondritis
  JOURNAL   Orphanet J Rare Dis 19 (1), 1 (2024)
   PUBMED   38167209
  REMARK    GeneRIF: Dynamic monitoring of UBA1 somatic mutations in patients
            with relapsing polychondritis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3572)
  AUTHORS   Baumbach-Reardon,L., Hunter,J.M., Ahearn,M.E. and Pfautsch,M.
  TITLE     Spinal Muscular Atrophy, X-Linked Infantile
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301739
REFERENCE   7  (bases 1 to 3572)
  AUTHORS   Schwartz,A.L., Trausch,J.S., Ciechanover,A., Slot,J.W. and Geuze,H.
  TITLE     Immunoelectron microscopic localization of the ubiquitin-activating
            enzyme E1 in HepG2 cells
  JOURNAL   Proc Natl Acad Sci U S A 89 (12), 5542-5546 (1992)
   PUBMED   1376922
REFERENCE   8  (bases 1 to 3572)
  AUTHORS   Ayusawa,D., Kaneda,S., Itoh,Y., Yasuda,H., Murakami,Y.,
            Sugasawa,K., Hanaoka,F. and Seno,T.
  TITLE     Complementation by a cloned human ubiquitin-activating enzyme E1 of
            the S-phase-arrested mouse FM3A cell mutant with thermolabile E1
  JOURNAL   Cell Struct Funct 17 (2), 113-122 (1992)
   PUBMED   1606621
REFERENCE   9  (bases 1 to 3572)
  AUTHORS   Takahashi,E., Ayusawa,D., Kaneda,S., Itoh,Y., Seno,T. and Hori,T.
  TITLE     The human ubiquitin-activating enzyme E1 gene (UBE1) mapped to band
            Xp11.3----p11.23 by fluorescence in situ hybridization
  JOURNAL   Cytogenet Cell Genet 59 (4), 268-269 (1992)
   PUBMED   1544321
REFERENCE   10 (bases 1 to 3572)
  AUTHORS   Kudo,M., Sugasawa,K., Hori,T., Enomoto,T., Hanaoka,F. and Ui,M.
  TITLE     Human ubiquitin-activating enzyme (E1): compensation for
            heat-labile mouse E1 and its gene localization on the X chromosome
  JOURNAL   Exp Cell Res 192 (1), 110-117 (1991)
   PUBMED   1845793
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI547207.1, M58028.1 and
            BC013041.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003334.3.
            
            Summary: The protein encoded by this gene catalyzes the first step
            in ubiquitin conjugation to mark cellular proteins for degradation.
            This gene complements an X-linked mouse temperature-sensitive
            defect in DNA synthesis, and thus may function in DNA repair. It is
            part of a gene cluster on chromosome Xp11.23. Alternatively spliced
            transcript variants that encode the same protein have been
            described. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.128662.1,
                                           SRR1660807.76023.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000335972.11/ ENSP00000338413.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-93                BI547207.1         33-125
            94-2919             M58028.1           27-2852
            2920-3572           BC013041.2         2814-3466
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp11.3"
     gene            1..3572
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /note="ubiquitin like modifier activating enzyme 1"
                     /db_xref="GeneID:7317"
                     /db_xref="HGNC:HGNC:12469"
                     /db_xref="MIM:314370"
     exon            1..196
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     CDS             197..3373
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /EC_number="6.2.1.45"
                     /note="POC20 centriolar protein homolog; UBA1,
                     ubiquitin-activating enzyme E1 homolog A; A1S9T and BN75
                     temperature sensitivity complementing; testicular
                     secretory protein Li 63"
                     /codon_start=1
                     /product="ubiquitin-like modifier-activating enzyme 1"
                     /protein_id="NP_003325.2"
                     /db_xref="CCDS:CCDS14275.1"
                     /db_xref="GeneID:7317"
                     /db_xref="HGNC:HGNC:12469"
                     /db_xref="MIM:314370"
                     /translation="MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKN
                     GSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLH
                     DQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQ
                     VVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLS
                     AMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFS
                     ICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQ
                     ALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAG
                     DLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNR
                     YDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTI
                     EKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQ
                     NLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDP
                     PEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGT
                     QPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAP
                     FWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPE
                     FTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSN
                     FHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQ
                     GHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTL
                     KQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRH
                     VRALVLELCCNDESGEDVEVPYVRYTIR"
     exon            197..313
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            314..372
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            373..541
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            542..676
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            677..783
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            784..874
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1007
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1105
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1252
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1429
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1534
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1615
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1771
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..1937
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2134
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..2199
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2395
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2470
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            2471..2660
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            2661..2749
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            2750..2842
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            2843..3034
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            3035..3136
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            3137..3237
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     exon            3238..3572
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3551..3556
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /note="hexamer: AATAAA"
     polyA_site      3572
                     /gene="UBA1"
                     /gene_synonym="A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;
                     POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS"
                     /note="major polyA site"
ORIGIN      
        1 atcttgtgtc ggcggctcgg ctgtaaggag gtggcaggga caaccacaac cacaacggcc
       61 gggggaggag aaggcggcag cggcgattct aggcggccca ggcggcgggg aggaggagaa
      121 ggaggagggt ggcggccggg cttggcttcg gctccttgag gagttggcgg cggcgcgacc
      181 cggggaaccg gcattgatgt ccagctcgcc gctgtccaag aaacgtcgcg tgtccgggcc
      241 tgatccaaag ccgggttcta actgctcccc tgcccagtcc gtgttgtccg aagtgccctc
      301 ggtgccaacc aacggaatgg ccaagaacgg cagtgaagca gacatagacg agggccttta
      361 ctcccggcag ctgtatgtgt tgggccatga ggcaatgaag cggctccaga catccagtgt
      421 cctggtatca ggcctgcggg gcctgggcgt ggagatcgct aagaacatca tccttggtgg
      481 ggtcaaggct gttaccctac atgaccaggg cactgcccag tgggctgatc tttcctccca
      541 gttctacctg cgggaggagg acatcggtaa aaaccgggcc gaggtatcac agccccgcct
      601 cgctgagctc aacagctatg tgcctgtcac tgcctacact ggacccctcg ttgaggactt
      661 ccttagtggt ttccaggtgg tggtgctcac caacaccccc ctggaggacc agctgcgagt
      721 gggtgagttc tgtcacaacc gtggcatcaa gctggtggtg gcagacacgc ggggcctgtt
      781 tgggcagctc ttctgtgact ttggagagga aatgatcctc acagattcca atggggagca
      841 gccactcagt gctatggttt ctatggttac caaggacaac cccggtgtgg ttacctgcct
      901 ggatgaggcc cgacacgggt ttgagagcgg ggactttgtc tccttttcag aagtacaggg
      961 catggttgaa ctcaacggaa atcagcccat ggagatcaaa gtcctgggtc cttatacctt
     1021 tagcatctgt gacacctcca acttctccga ctacatccgt ggaggcatcg tcagtcaggt
     1081 caaagtacct aagaagatta gctttaaatc cttggtggcc tcactggcag aacctgactt
     1141 tgtggtgacg gacttcgcca agttttctcg ccctgcccag ctgcacattg gcttccaggc
     1201 cctgcaccag ttctgtgctc agcatggccg gccacctcgg ccccgcaatg aggaggatgc
     1261 agcagaactg gtagccttag cacaggctgt gaatgctcga gccctgccag cagtgcagca
     1321 aaataacctg gacgaggacc tcatccggaa gctggcatat gtggctgctg gggatctggc
     1381 acccataaac gccttcattg ggggcctggc tgcccaggaa gtcatgaagg cctgctccgg
     1441 gaagttcatg cccatcatgc agtggctata ctttgatgcc cttgagtgtc tccctgagga
     1501 caaagaggtc ctcacagagg acaagtgcct ccagcgccag aaccgttatg acgggcaagt
     1561 ggctgtgttt ggctcagacc tgcaagagaa gctgggcaag cagaagtatt tcctggtggg
     1621 tgcgggggcc attggctgtg agctgctcaa gaactttgcc atgattgggc tgggctgcgg
     1681 ggagggtgga gaaatcatcg ttacagacat ggacaccatt gagaagtcaa atctgaatcg
     1741 acagtttctt ttccggccct gggatgtcac gaagttaaag tctgacacgg ctgctgcagc
     1801 tgtgcgccaa atgaatccac atatccgggt gacaagccac cagaaccgtg tgggtcctga
     1861 cacggagcgc atctatgatg acgatttttt ccaaaaccta gatggcgtgg ccaatgccct
     1921 ggacaacgtg gatgcccgca tgtacatgga ccgccgctgt gtctactacc ggaagccact
     1981 gctggagtca ggcacactgg gcaccaaagg caatgtgcag gtggtgatcc ccttcctgac
     2041 agagtcgtac agttccagcc aggacccacc tgagaagtcc atccccatct gtaccctgaa
     2101 gaacttccct aatgccatcg agcacaccct gcagtgggct cgggatgagt ttgaaggcct
     2161 cttcaagcag ccagcagaaa atgtcaacca gtacctcaca gaccccaagt ttgtggagcg
     2221 aacactgcgg ctggcaggca ctcagccctt ggaggtgctg gaggctgtgc agcgcagcct
     2281 ggtgctgcag cgaccacaga cctgggctga ctgcgtgacc tgggcctgcc accactggca
     2341 cacccagtac tcgaacaaca tccggcagct gctgcacaac ttccctcctg accagctcac
     2401 aagctcagga gcgccgttct ggtctgggcc caaacgctgt ccacacccgc tcacctttga
     2461 tgtcaacaat cccctgcatc tggactatgt gatggctgct gccaacctgt ttgcccagac
     2521 ctacgggctg acaggctctc aggaccgagc tgctgtggcc acattcctgc agtctgtgca
     2581 ggtccccgaa ttcaccccca agtctggcgt caagatccat gtttctgacc aggagctgca
     2641 gagcgccaat gcctctgttg atgacagtcg tctagaggag ctcaaagcca ctctgcccag
     2701 cccagacaag ctccctggat tcaagatgta ccccattgac tttgagaagg atgatgacag
     2761 caactttcat atggatttca tcgtggctgc atccaacctc cgggcagaaa actatgacat
     2821 tccttctgca gaccggcaca agagcaagct gattgcaggg aagatcatcc cagccattgc
     2881 cacgaccaca gcagccgtgg ttggccttgt gtgtctggag ctgtacaagg ttgtgcaggg
     2941 gcaccgacag cttgactcct acaagaatgg tttcctcaac ttggccctgc ctttctttgg
     3001 tttctctgaa ccccttgccg caccacgtca ccagtactat aaccaagagt ggacattgtg
     3061 ggatcgcttt gaggtacaag ggctgcagcc taatggtgag gagatgaccc tcaaacagtt
     3121 cctcgactat tttaagacag agcacaaatt agagatcacc atgctgtccc agggcgtgtc
     3181 catgctctat tccttcttca tgccagctgc caagctcaag gaacggttgg atcagccgat
     3241 gacagagatt gtgagccgtg tgtcgaagcg aaagctgggc cgccacgtgc gggcgctggt
     3301 gcttgagctg tgctgtaacg acgagagcgg cgaggatgtc gaggttccct atgtccgata
     3361 caccatccgc tgaccccgtc tgctcctcta ggctggcccc ttgtccaccc ctctccacac
     3421 cccttccagc ccagggttcc catttggctt ctggcagtgg cccaactagc caagtctggt
     3481 gttccctcat catcccccta cctgaacccc tcttgccact gccttctacc ttgtttgaaa
     3541 cctgaatcct aataaagaat taataactcc ca
//
