LOCUS       NM_018264               3330 bp    mRNA    linear   PRI 10-FEB-2024
DEFINITION  Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1),
            transcript variant 1, mRNA.
ACCESSION   NM_018264
VERSION     NM_018264.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Noma,A. and Suzuki,T.
  TITLE     Ribonucleome analysis identified enzyme genes responsible for
            wybutosine synthesis
  JOURNAL   Nucleic Acids Symp Ser (Oxf) (50), 65-66 (2006)
   PUBMED   17150819
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Waas,W.F., de Crecy-Lagard,V. and Schimmel,P.
  TITLE     Discovery of a gene family critical to wyosine base formation in a
            subset of phenylalanine-specific transfer RNAs
  JOURNAL   J Biol Chem 280 (45), 37616-37622 (2005)
   PUBMED   16162496
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC079920.6, AC073089.5 and
            AC006480.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_018264.3.
            
            Summary: Wybutosine (yW) is a hypermodified guanosine found in
            phenylalanine tRNA adjacent to the anticodon that stabilizes
            codon-anticodon interactions in the ribosome. In yeast, the homolog
            of this gene is essential for the synthesis of wybutosine.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the functional protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC015591.2, SRR1660809.63784.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000359626.10/ ENSP00000352645.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-150               AC079920.6         60391-60540
            151-281             AC079920.6         61623-61753
            282-419             AC079920.6         62375-62512
            420-521             AC079920.6         73141-73242
            522-716             AC079920.6         77925-78119
            717-1007            AC079920.6         81411-81701
            1008-1130           AC079920.6         88458-88580
            1131-1248           AC079920.6         113507-113624
            1249-1301           AC079920.6         119393-119445
            1302-1420           AC073089.5         2559-2677
            1421-1530           AC073089.5         18704-18813
            1531-1708           AC073089.5         33815-33992
            1709-1844           AC073089.5         52757-52892
            1845-1955           AC073089.5         118400-118510
            1956-2123           AC073089.5         130444-130611
            2124-3330           AC006480.3         3794-5000
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q11.21"
     gene            1..3330
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /note="tRNA-yW synthesizing protein 1 homolog"
                     /db_xref="GeneID:55253"
                     /db_xref="HGNC:HGNC:25598"
                     /db_xref="MIM:611243"
     exon            1..150
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..2345
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /EC_number="4.1.3.44"
                     /note="radical S-adenosyl methionine and flavodoxin
                     domains 1; tRNA-yW synthesizing protein 1 homolog A; tRNA
                     wybutosine-synthesizing protein 1 homolog; radical
                     S-adenosyl methionine and flavodoxin domain-containing
                     protein 1"
                     /codon_start=1
                     /product="S-adenosyl-L-methionine-dependent tRNA
                     4-demethylwyosine synthase TYW1"
                     /protein_id="NP_060734.2"
                     /db_xref="CCDS:CCDS5538.1"
                     /db_xref="GeneID:55253"
                     /db_xref="HGNC:HGNC:25598"
                     /db_xref="MIM:611243"
                     /translation="MDPSADTWDLFSPLISLWINRFYIYLGFAVSISLWICVQIVIKT
                     QGKNLQEKSVPKAAQDLMTNGYVSLQEKDIFVSGVKIFYGSQTGTAKGFATVLAEAVT
                     SLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASI
                     DFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVV
                     KSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSH
                     EQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQ
                     EEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRG
                     GCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAI
                     ENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISS
                     FLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVK
                     QQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWH
                     EEVVQFVHELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEY
                     EDSGGSKTFSAKDYMARTPHWALFGASERGFDPKDTRHQRKNKSKAISGC"
     exon            151..281
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            282..419
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            420..521
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            522..716
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            717..1007
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1130
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1248
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1301
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1420
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1530
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1708
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            1709..1844
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1955
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2123
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..3330
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3308..3313
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /note="hexamer: AATAAA"
     polyA_site      3330
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /note="major polyA site"
ORIGIN      
        1 ctggcagtgt catggctgcc cacaggtctg caggcactcg gtacgccgct aacgcggcga
       61 ggtagctcgg tgcgtctcgc ggtaccagtg cgaatcatcg ggctatccag gtccgagatc
      121 ctagtctcct gtcggctctg aggaggatgg atccttctgc ggatacatgg gacctcttct
      181 cacctttaat atcattatgg ataaacaggt tttacattta tttgggcttt gctgttagca
      241 ttagcctttg gatttgtgtc cagattgtca tcaagacgca gggcaagaac ttacaggaaa
      301 aatctgttcc aaaagcagct caggatttga tgacaaatgg ttatgtctcc cttcaagaga
      361 aagacatctt tgtgtctgga gtgaagattt tttatggttc tcagactgga acagcgaagg
      421 gattcgcaac agttcttgct gaagcagtta catccctgga tctgcctgtg gccattatta
      481 atctaaaaga atatgatcca gatgatcatc tgatagaaga ggtgactagt aaaaatgtct
      541 gtgtcttcct ggttgcgaca tacactgacg gcctaccaac tgaaagtgca gagtggttct
      601 gcaaatggtt agaggaagca tccattgatt ttcgatttgg caaaacttac ctgaagggta
      661 tgagatatgc ggtatttggc ctgggaaatt ctgcctatgc tagccacttc aacaaggttg
      721 gcaaaaatgt tgacaagtgg ctctggatgc ttggcgcgca tcgtgtgatg agtcgagggg
      781 agggcgactg cgacgtggtt aaaagcaagc acggcagcat tgaggccgac ttcagagcat
      841 ggaagaccaa gttcatctcc cagctgcagg cacttcagaa aggggagaga aagaagtcct
      901 gtggcggcca ctgcaagaaa ggcaaatgtg aatctcacca acatggctca gaggagaggg
      961 aggaaggatc tcatgagcag gatgaattgc atcatagaga caccgaggag gaagaaccct
     1021 ttgagagctc cagtgaagaa gagtttggtg gtgaggacca tcagagccta aattccattg
     1081 ttgatgttga agatttgggc aaaattatgg atcatgtgaa gaaagaaaag agagaaaagg
     1141 aacagcagga agagaagtct ggtttgttca ggaacatggg gaggaatgaa gatggtgaaa
     1201 gaagagctat gataactcct gctctccgag aagcccttac taaacaaggt tatcagttga
     1261 ttgggagcca ctcgggggtg aagctttgca ggtggacaaa gtccatgctc cgagggagag
     1321 gaggttgtta caaacacaca ttctatggaa ttgagagcca tcgctgcatg gaaaccaccc
     1381 cgagcttggc gtgtgctaat aaatgtgtct tctgttggcg gcaccacacc aaccccgtgg
     1441 gcactgagtg gcggtggaag atggaccagc ctgaaatgat cttgaaggaa gccattgaaa
     1501 accatcagaa catgattaag cagtttaaag gagtaccggg cgtcaaagca gaacgctttg
     1561 aagaaggaat gacggtaaag cactgtgcat tgtccctcgt gggagaacca ataatgtacc
     1621 cagagatcaa caggtttttg aagctactcc accagtgtaa aatttccagc ttcctggtca
     1681 caaacgcaca atttcctgcg gaaatcagga acctcgagcc ggttactcag ctgtatgtca
     1741 gtgtggatgc cagtaccaaa gacagcctga agaaaatcga ccgcccactc ttcaaggatt
     1801 tctggcagag attccttgac agtttaaaag ccttggcagt caagcaacaa cgaactgtct
     1861 acagactgac gctcgtgaaa gcatggaacg tggacgagct ccaggcctac gcgcagctcg
     1921 tgtccctggg gaatcctgac ttcatcgaag tgaagggcgt tacctactgc ggagaaagtt
     1981 cagcaagcag tcttaccatg gcccacgtgc cctggcatga ggaagtggta cagtttgtcc
     2041 acgagttggt ggatctgatc cccgaatatg aaattgcatg tgaacacgaa cactctaatt
     2101 gcctcctgat agcacacaga aagtttaaaa ttggtggtga atggtggaca tggatcgatt
     2161 ataaccgctt ccaggagctc atccaggaat atgaagatag tggtggatca aaaacgttca
     2221 gcgcaaagga ttatatggcc agaactcctc actgggcatt atttggtgcc agtgaaagag
     2281 gctttgatcc caaggacaca agacatcaga gaaagaacaa atcaaaggct atttctggat
     2341 gttgagatta tctgatttca aggtactgaa ggacaaaaac ttggatggcc tcaaaaggtt
     2401 cttgaacacc actgtgattc tccaaggacg aattacgtaa attatacttt catacaaagg
     2461 agacgataag gcagtaaaca tggagacacg ggggacagcg tccacactca gagggcctgg
     2521 gccacagccc cgatgtttct tttcagaact cagccccttt cctgatttta cttctaagag
     2581 gaaaattatt ttggggagga actacacagt cgtgattaga atttatctga tggttttgta
     2641 ttataacttg taagacctgc cagaatgcta gtcccgagag tgtcagacaa ggaagaagtc
     2701 cctgggcctc ttccccttac ccggccctta gatttcatgg agcagccact tagcattgaa
     2761 ttgcactacc ctgagctaaa cgtgtctgtg ctttctaaga taagagcttg atccctttct
     2821 tctatcttaa gacagcacct cctgaaaaga atcgaagttg tcacaactct caattatttt
     2881 ttaaatactg catagattga gttttggttt attaccaacc cttcccagaa ttgcgttgga
     2941 tctaaaacta ctagatctca tcccattccc atgtaaatta ccacagaccg cagtaccggg
     3001 gctggagcgg agtgaagctg tctgctgtaa gaggagtggc catgtgaggg catggagtca
     3061 ttagtctcac aaacacactt tggactgaag aggatcattt ctttttgttc gtgaggtcac
     3121 tgtccaggcc tctcatatca tgaccagacg gcgggtctcc atcttctttc actcctgtgg
     3181 ccctggctgc tttacacaat ctgttctata aggttcaggt gttttcaagt tggaaagatc
     3241 ataaatactc aaaattgttt tcaagttagc aagttctttt aacagtcttt tatgcaaaaa
     3301 ttgaattaat aaaataatct tttgtaaaga
//
