LOCUS       NP_060734                732 aa            linear   PRI 28-APR-2025
DEFINITION  S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            TYW1 [Homo sapiens].
ACCESSION   NP_060734
VERSION     NP_060734.2
DBSOURCE    REFSEQ: accession NM_018264.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 732)
  AUTHORS   Noma,A. and Suzuki,T.
  TITLE     Ribonucleome analysis identified enzyme genes responsible for
            wybutosine synthesis
  JOURNAL   Nucleic Acids Symp Ser (Oxf) (50), 65-66 (2006)
   PUBMED   17150819
REFERENCE   2  (residues 1 to 732)
  AUTHORS   Waas,W.F., de Crecy-Lagard,V. and Schimmel,P.
  TITLE     Discovery of a gene family critical to wyosine base formation in a
            subset of phenylalanine-specific transfer RNAs
  JOURNAL   J Biol Chem 280 (45), 37616-37622 (2005)
   PUBMED   16162496
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC079920.6, AC073089.5 and
            AC006480.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 28, 2004 this sequence version replaced NP_060734.1.
            
            Summary: Wybutosine (yW) is a hypermodified guanosine found in
            phenylalanine tRNA adjacent to the anticodon that stabilizes
            codon-anticodon interactions in the ribosome. In yeast, the homolog
            of this gene is essential for the synthesis of wybutosine.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the functional protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC015591.2, SRR1660809.63784.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000359626.10/ ENSP00000352645.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q11.21"
     Protein         1..732
                     /product="S-adenosyl-L-methionine-dependent tRNA
                     4-demethylwyosine synthase TYW1"
                     /EC_number="4.1.3.44"
                     /note="radical S-adenosyl methionine and flavodoxin
                     domains 1; tRNA-yW synthesizing protein 1 homolog A; tRNA
                     wybutosine-synthesizing protein 1 homolog; radical
                     S-adenosyl methionine and flavodoxin domain-containing
                     protein 1"
                     /calculated_mol_wt=83571
     Region          81..>246
                     /region_name="CysJ"
                     /note="Flavoprotein (flavin reductase) subunit CysJ of
                     sulfite and N-hydroxylaminopurine reductases [Nucleotide
                     transport and metabolism, Inorganic ion transport and
                     metabolism]; COG0369"
                     /db_xref="CDD:440138"
     Region          248..314
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NV66.2)"
     Region          360..656
                     /region_name="rSAM_TYW1"
                     /note="wyosine biosynthesis protein TYW1; TIGR03972"
                     /db_xref="CDD:274890"
     CDS             1..732
                     /gene="TYW1"
                     /gene_synonym="RSAFD1; TYW1A; YPL207W"
                     /coded_by="NM_018264.4:147..2345"
                     /db_xref="CCDS:CCDS5538.1"
                     /db_xref="GeneID:55253"
                     /db_xref="HGNC:HGNC:25598"
                     /db_xref="MIM:611243"
ORIGIN      
        1 mdpsadtwdl fsplislwin rfyiylgfav sislwicvqi viktqgknlq eksvpkaaqd
       61 lmtngyvslq ekdifvsgvk ifygsqtgta kgfatvlaea vtsldlpvai inlkeydpdd
      121 hlieevtskn vcvflvatyt dglptesaew fckwleeasi dfrfgktylk gmryavfglg
      181 nsayashfnk vgknvdkwlw mlgahrvmsr gegdcdvvks khgsieadfr awktkfisql
      241 qalqkgerkk scgghckkgk ceshqhgsee reegsheqde lhhrdteeee pfessseeef
      301 ggedhqslns ivdvedlgki mdhvkkekre keqqeeksgl frnmgrnedg erramitpal
      361 realtkqgyq ligshsgvkl crwtksmlrg rggcykhtfy gieshrcmet tpslacankc
      421 vfcwrhhtnp vgtewrwkmd qpemilkeai enhqnmikqf kgvpgvkaer feegmtvkhc
      481 alslvgepim ypeinrflkl lhqckissfl vtnaqfpaei rnlepvtqly vsvdastkds
      541 lkkidrplfk dfwqrfldsl kalavkqqrt vyrltlvkaw nvdelqayaq lvslgnpdfi
      601 evkgvtycge ssassltmah vpwheevvqf vhelvdlipe yeiacehehs nclliahrkf
      661 kiggewwtwi dynrfqeliq eyedsggskt fsakdymart phwalfgase rgfdpkdtrh
      721 qrknkskais gc
//
