LOCUS       NM_001071               1613 bp    mRNA    linear   PRI 13-NOV-2024
DEFINITION  Homo sapiens thymidylate synthetase (TYMS), transcript variant 1,
            mRNA.
ACCESSION   NM_001071
VERSION     NM_001071.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Zhang,S., Liu,N., Cao,P., Qin,Q., Li,J., Yang,L., Xin,Y., Jiang,M.,
            Zhang,S., Yang,J. and Lu,J.
  TITLE     LncRNA BC200 promotes the development of EBV-associated
            nasopharyngeal carcinoma by competitively binding to miR-6834-5p to
            upregulate TYMS expression
  JOURNAL   Int J Biol Macromol 278 (Pt 4), 134837 (2024)
   PUBMED   39179085
  REMARK    GeneRIF: LncRNA BC200 promotes the development of EBV-associated
            nasopharyngeal carcinoma by competitively binding to miR-6834-5p to
            upregulate TYMS expression.
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Kim,J.O., Ryu,C.S., Lee,J.Y., Ko,E.J., Ha,Y.H., Sung,J.H.,
            Hwang,T.S., Kim,I.J. and Kim,N.K.
  TITLE     Association of Thymidylate Synthase (TS) Gene Polymorphisms with
            Incidence and Prognosis of Coronary Artery Disease
  JOURNAL   Int J Mol Sci 24 (16), 12591 (2023)
   PUBMED   37628769
  REMARK    GeneRIF: Association of Thymidylate Synthase (TS) Gene
            Polymorphisms with Incidence and Prognosis of Coronary Artery
            Disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1613)
  AUTHORS   Hisatomi,H., Tanemura,H., Iizuka,T., Katsumata,K., Nagao,K.,
            Sumida,H., Udagawa,H. and Hikiji,K.
  TITLE     Differential alternative splicing expressions of thymidylate
            synthase isoforms
  JOURNAL   Cancer Lett 193 (2), 127-131 (2003)
   PUBMED   12706868
REFERENCE   4  (bases 1 to 1613)
  AUTHORS   Chu,J. and Dolnick,B.J.
  TITLE     Natural antisense (rTSalpha) RNA induces site-specific cleavage of
            thymidylate synthase mRNA
  JOURNAL   Biochim Biophys Acta 1587 (2-3), 183-193 (2002)
   PUBMED   12084460
  REMARK    GeneRIF: rTS gene expression likely plays a role in down-regulating
            TS through a natural RNA-based antisense mechanism. Review.
            Review article
REFERENCE   5  (bases 1 to 1613)
  AUTHORS   Schiffer,C.A., Clifton,I.J., Davisson,V.J., Santi,D.V. and
            Stroud,R.M.
  TITLE     Crystal structure of human thymidylate synthase: a structural
            mechanism for guiding substrates into the active site
  JOURNAL   Biochemistry 34 (50), 16279-16287 (1995)
   PUBMED   8845352
REFERENCE   6  (bases 1 to 1613)
  AUTHORS   Johnston,P.G., Liang,C.M., Henry,S., Chabner,B.A. and Allegra,C.J.
  TITLE     Production and characterization of monoclonal antibodies that
            localize human thymidylate synthase in the cytoplasm of human cells
            and tissue
  JOURNAL   Cancer Res 51 (24), 6668-6676 (1991)
   PUBMED   1720706
REFERENCE   7  (bases 1 to 1613)
  AUTHORS   Chu,E., Koeller,D.M., Casey,J.L., Drake,J.C., Chabner,B.A.,
            Elwood,P.C., Zinn,S. and Allegra,C.J.
  TITLE     Autoregulation of human thymidylate synthase messenger RNA
            translation by thymidylate synthase
  JOURNAL   Proc Natl Acad Sci U S A 88 (20), 8977-8981 (1991)
   PUBMED   1924359
REFERENCE   8  (bases 1 to 1613)
  AUTHORS   Kaneda,S., Nalbantoglu,J., Takeishi,K., Shimizu,K., Gotoh,O.,
            Seno,T. and Ayusawa,D.
  TITLE     Structural and functional analysis of the human thymidylate
            synthase gene
  JOURNAL   J Biol Chem 265 (33), 20277-20284 (1990)
   PUBMED   2243092
REFERENCE   9  (bases 1 to 1613)
  AUTHORS   Takeishi,K., Kaneda,S., Ayusawa,D., Shimizu,K., Gotoh,O. and
            Seno,T.
  TITLE     Human thymidylate synthase gene: isolation of phage clones which
            cover a functionally active gene and structural analysis of the
            region upstream from the translation initiation codon
  JOURNAL   J Biochem 106 (4), 575-583 (1989)
   PUBMED   2532645
REFERENCE   10 (bases 1 to 1613)
  AUTHORS   Takeishi,K., Kaneda,S., Ayusawa,D., Shimizu,K., Gotoh,O. and
            Seno,T.
  TITLE     Nucleotide sequence of a functional cDNA for human thymidylate
            synthase
  JOURNAL   Nucleic Acids Res 13 (6), 2035-2043 (1985)
   PUBMED   2987839
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY016886.1, AK223372.1,
            BC018858.2, BC083512.1 and HY269620.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001071.3.
            
            Summary: Thymidylate synthase catalyzes the methylation of
            deoxyuridylate to deoxythymidylate using,
            10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This
            function maintains the dTMP (thymidine-5-prime monophosphate) pool
            critical for DNA replication and repair. The enzyme has been of
            interest as a target for cancer chemotherapeutic agents. It is
            considered to be the primary site of action for 5-fluorouracil,
            5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression
            of this gene and that of a naturally occurring antisense
            transcript, mitochondrial enolase superfamily member 1
            (GeneID:55556), vary inversely when cell-growth progresses from
            late-log to plateau phase. Polymorphisms in this gene may be
            associated with etiology of neoplasia, including breast cancer, and
            response to chemotherapy. [provided by RefSeq, Aug 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.118090.1,
                                           SRR1163658.307472.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000323274.15/ ENSP00000315644.10
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                HY016886.1         73-104
            33-38               AK223372.1         70-75
            39-1050             BC018858.2         1-1012
            1051-1534           BC083512.1         1067-1550
            1535-1613           HY269620.1         29-107              c
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.32"
     gene            1..1613
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /note="thymidylate synthetase"
                     /db_xref="GeneID:7298"
                     /db_xref="HGNC:HGNC:12441"
                     /db_xref="MIM:188350"
     exon            1..295
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..1032
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /EC_number="2.1.1.45"
                     /note="isoform 1 is encoded by transcript variant 1;
                     TSase"
                     /codon_start=1
                     /product="thymidylate synthase isoform 1"
                     /protein_id="NP_001062.1"
                     /db_xref="CCDS:CCDS11821.1"
                     /db_xref="GeneID:7298"
                     /db_xref="HGNC:HGNC:12441"
                     /db_xref="MIM:188350"
                     /translation="MPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCG
                     VRKDDRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELS
                     SKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQ
                     LQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGD
                     MGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPF
                     PKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV"
     exon            296..369
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /inference="alignment:Splign:2.1.0"
     exon            370..544
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /inference="alignment:Splign:2.1.0"
     exon            545..646
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /inference="alignment:Splign:2.1.0"
     exon            647..822
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /inference="alignment:Splign:2.1.0"
     exon            823..894
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1613
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1510..1515
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /note="hexamer: AATAAA"
     polyA_site      1534
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /note="major polyA site"
     regulatory      1590..1595
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
                     /note="hexamer: ATTAAA"
     polyA_site      1613
                     /gene="TYMS"
                     /gene_synonym="DKCD; HST422; TMS; TS"
ORIGIN      
        1 acttggcctg cctccgtccc gccgcgccac ttggcctgcc tccgtcccgc cgcgccactt
       61 cgcctgcctc cgtcccccgc ccgccgcgcc atgcctgtgg ccggctcgga gctgccgcgc
      121 cggcccttgc cccccgccgc acaggagcgg gacgccgagc cgcgtccgcc gcacggggag
      181 ctgcagtacc tggggcagat ccaacacatc ctccgctgcg gcgtcaggaa ggacgaccgc
      241 acgggcaccg gcaccctgtc ggtattcggc atgcaggcgc gctacagcct gagagatgaa
      301 ttccctctgc tgacaaccaa acgtgtgttc tggaagggtg ttttggagga gttgctgtgg
      361 tttatcaagg gatccacaaa tgctaaagag ctgtcttcca agggagtgaa aatctgggat
      421 gccaatggat cccgagactt tttggacagc ctgggattct ccaccagaga agaaggggac
      481 ttgggcccag tttatggctt ccagtggagg cattttgggg cagaatacag agatatggaa
      541 tcagattatt caggacaggg agttgaccaa ctgcaaagag tgattgacac catcaaaacc
      601 aaccctgacg acagaagaat catcatgtgc gcttggaatc caagagatct tcctctgatg
      661 gcgctgcctc catgccatgc cctctgccag ttctatgtgg tgaacagtga gctgtcctgc
      721 cagctgtacc agagatcggg agacatgggc ctcggtgtgc ctttcaacat cgccagctac
      781 gccctgctca cgtacatgat tgcgcacatc acgggcctga agccaggtga ctttatacac
      841 actttgggag atgcacatat ttacctgaat cacatcgagc cactgaaaat tcagcttcag
      901 cgagaaccca gacctttccc aaagctcagg attcttcgaa aagttgagaa aattgatgac
      961 ttcaaagctg aagactttca gattgaaggg tacaatccgc atccaactat taaaatggaa
     1021 atggctgttt agggtgcttt caaaggagct cgaaggatat tgtcagtctt taggggttgg
     1081 gctggatgcc gaggtaaaag ttctttttgc tctaaaagaa aaaggaacta ggtcaaaaat
     1141 ctgtccgtga cctatcagtt attaattttt aaggatgttg ccactggcaa atgtaactgt
     1201 gccagttctt tccataataa aaggctttga gttaactcac tgagggtatc tgacaatgct
     1261 gaggttatga acaaagtgag gagaatgaaa tgtatgtgct cttagcaaaa acatgtatgt
     1321 gcatttcaat cccacgtact tataaagaag gttggtgaat ttcacaagct atttttggaa
     1381 tatttttaga atattttaag aatttcacaa gctattccct caaatctgag ggagctgagt
     1441 aacaccatcg atcatgatgt agagtgtggt tatgaacttt aaagttatag ttgttttata
     1501 tgttgctata ataaagaagt gttctgcatt cgtccacgct ttgttcattc tgtactgcca
     1561 cttatctgct cagttccttc ctaaaataga ttaaagaact ctccttaagt aaa
//
