LOCUS       NM_052883               2921 bp    mRNA    linear   PRI 13-FEB-2025
DEFINITION  Homo sapiens thioredoxin reductase 3 (TXNRD3), transcript variant
            1, mRNA.
ACCESSION   NM_052883
VERSION     NM_052883.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   He,A.Y., Zhao,X., Hao,J., Shi,C.D., Liu,Q.L., Sun,N., Qu,C.Y. and
            Zhang,R.Q.
  TITLE     [Effect of Selenoprotein Thioredoxin Reductase 3 on the Survival
            Prognosis of Tumor Patients]
  JOURNAL   Zhongguo Yi Xue Ke Xue Yuan Xue Bao 44 (6), 970-979 (2022)
   PUBMED   36621786
  REMARK    GeneRIF: [Effect of Selenoprotein Thioredoxin Reductase 3 on the
            Survival Prognosis of Tumor Patients].
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2921)
  AUTHORS   Brandstaedter,C., Fritz-Wolf,K., Weder,S., Fischer,M., Hecker,B.,
            Rahlfs,S. and Becker,K.
  TITLE     Kinetic characterization of wild-type and mutant human thioredoxin
            glutathione reductase defines its reaction and regulatory
            mechanisms
  JOURNAL   FEBS J 285 (3), 542-558 (2018)
   PUBMED   29222842
  REMARK    GeneRIF: Findings represent the first characterization of hTGR and
            provide new insights into the reaction mechanism and the regulation
            of monothiol Grx-containing thioredoxin glutathione reductases.
REFERENCE   4  (bases 1 to 2921)
  AUTHORS   Gladyshev,V.N., Arner,E.S., Berry,M.J., Brigelius-Flohe,R.,
            Bruford,E.A., Burk,R.F., Carlson,B.A., Castellano,S., Chavatte,L.,
            Conrad,M., Copeland,P.R., Diamond,A.M., Driscoll,D.M., Ferreiro,A.,
            Flohe,L., Green,F.R., Guigo,R., Handy,D.E., Hatfield,D.L.,
            Hesketh,J., Hoffmann,P.R., Holmgren,A., Hondal,R.J., Howard,M.T.,
            Huang,K., Kim,H.Y., Kim,I.Y., Kohrle,J., Krol,A., Kryukov,G.V.,
            Lee,B.J., Lee,B.C., Lei,X.G., Liu,Q., Lescure,A., Lobanov,A.V.,
            Loscalzo,J., Maiorino,M., Mariotti,M., Sandeep Prabhu,K.,
            Rayman,M.P., Rozovsky,S., Salinas,G., Schmidt,E.E., Schomburg,L.,
            Schweizer,U., Simonovic,M., Sunde,R.A., Tsuji,P.A., Tweedie,S.,
            Ursini,F., Whanger,P.D. and Zhang,Y.
  TITLE     Selenoprotein Gene Nomenclature
  JOURNAL   J Biol Chem 291 (46), 24036-24040 (2016)
   PUBMED   27645994
REFERENCE   5  (bases 1 to 2921)
  AUTHORS   Gerashchenko,M.V., Su,D. and Gladyshev,V.N.
  TITLE     CUG start codon generates thioredoxin/glutathione reductase
            isoforms in mouse testes
  JOURNAL   J Biol Chem 285 (7), 4595-4602 (2010)
   PUBMED   20018845
  REMARK    GeneRIF: This study pertains to use of non-AUG (CUG) start codon in
            mouse thioredoxin reductase 3 gene, and conservation of this site
            in other mammals.
REFERENCE   6  (bases 1 to 2921)
  AUTHORS   Matsuzaka,Y., Okamoto,K., Mabuchi,T., Iizuka,M., Ozawa,A., Oka,A.,
            Tamiya,G., Kulski,J.K. and Inoko,H.
  TITLE     Identification and characterization of novel variants of the
            thioredoxin reductase 3 new transcript 1 TXNRD3NT1
  JOURNAL   Mamm Genome 16 (1), 41-49 (2005)
   PUBMED   15674732
REFERENCE   7  (bases 1 to 2921)
  AUTHORS   Matsuzaka,Y., Okamoto,K., Mabuchi,T., Iizuka,M., Ozawa,A., Oka,A.,
            Tamiya,G., Kulski,J.K. and Inoko,H.
  TITLE     Identification, expression analysis and polymorphism of a novel
            RLTPR gene encoding a RGD motif, tropomodulin domain and
            proline/leucine-rich regions
  JOURNAL   Gene 343 (2), 291-304 (2004)
   PUBMED   15588584
REFERENCE   8  (bases 1 to 2921)
  AUTHORS   Kryukov,G.V., Castellano,S., Novoselov,S.V., Lobanov,A.V.,
            Zehtab,O., Guigo,R. and Gladyshev,V.N.
  TITLE     Characterization of mammalian selenoproteomes
  JOURNAL   Science 300 (5624), 1439-1443 (2003)
   PUBMED   12775843
REFERENCE   9  (bases 1 to 2921)
  AUTHORS   Sun,Q.A., Kirnarsky,L., Sherman,S. and Gladyshev,V.N.
  TITLE     Selenoprotein oxidoreductase with specificity for thioredoxin and
            glutathione systems
  JOURNAL   Proc Natl Acad Sci U S A 98 (7), 3673-3678 (2001)
   PUBMED   11259642
REFERENCE   10 (bases 1 to 2921)
  AUTHORS   Sun,Q.A., Wu,Y., Zappacosta,F., Jeang,K.T., Lee,B.J., Hatfield,D.L.
            and Gladyshev,V.N.
  TITLE     Redox regulation of cell signaling by selenocysteine in mammalian
            thioredoxin reductases
  JOURNAL   J Biol Chem 274 (35), 24522-24530 (1999)
   PUBMED   10455115
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC024558.21.
            
            On Aug 14, 2020 this sequence version replaced NM_052883.2.
            
            Summary: The protein encoded by this gene belongs to the pyridine
            nucleotide-disulfide oxidoreductase family, and is a member of the
            thioredoxin (Trx) system. Three thioredoxin reductase (TrxR)
            isozymes are found in mammals. TrxRs are selenocysteine-containing
            flavoenzymes, which reduce thioredoxins, as well as other
            substrates, and play a key role in redox homoeostasis. This gene
            encodes the third TrxR, which unlike the other two isozymes,
            contains an additional N-terminal glutaredoxin (Grx) domain, and
            shows highest expression in testis. The Grx domain allows this
            isozyme to participate in both Trx and glutathione systems. It
            functions as a homodimer containing FAD, and selenocysteine (Sec)
            at the active site. Sec is encoded by UGA codon that normally
            signals translation termination. The 3' UTRs of selenoprotein mRNAs
            contain a conserved stem-loop structure, the Sec insertion sequence
            (SECIS) element, which is necessary for the recognition of UGA as a
            Sec codon rather than as a stop signal. Alternatively spliced
            transcript variants have been found for this gene. Experimental
            evidence suggests the use of a non-AUG (CUG) codon as a translation
            initiation codon (PMID:20018845). [provided by RefSeq, Aug 2017].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript, and encodes the longer isoform (1). A non-AUG (CUG)
            codon is used as translation initiation codon.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF171055.1, BC050032.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match              :: ENST00000524230.9/
                                               ENSP00000430031.4
            non-AUG initiation codon        :: inferred from conservation
            protein contains selenocysteine :: PMID: 10455115
            RefSeq Select criteria          :: based on conservation,
                                               expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-377               AC024558.21        215377-215753       c
            378-438             AC024558.21        207865-207925       c
            439-548             AC024558.21        206740-206849       c
            549-653             AC024558.21        204926-205030       c
            654-726             AC024558.21        204610-204682       c
            727-846             AC024558.21        202661-202780       c
            847-989             AC024558.21        194538-194680       c
            990-1105            AC024558.21        192393-192508       c
            1106-1331           AC024558.21        191341-191566       c
            1332-1424           AC024558.21        190008-190100       c
            1425-1501           AC024558.21        183093-183169       c
            1502-1658           AC024558.21        182371-182527       c
            1659-1766           AC024558.21        175984-176091       c
            1767-1862           AC024558.21        171666-171761       c
            1863-1997           AC024558.21        169128-169262       c
            1998-2921           AC024558.21        167679-168602       c
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.3"
     gene            1..2921
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /note="thioredoxin reductase 3"
                     /db_xref="GeneID:114112"
                     /db_xref="HGNC:HGNC:20667"
                     /db_xref="MIM:606235"
     exon            1..377
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..2066
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /EC_number="1.8.1.9"
                     /note="UGA stop codon recoded as selenocysteine; isoform 1
                     is encoded by transcript variant 1; non-AUG (CUG)
                     translation initiation codon; thioredoxin glutathione
                     reductase; thioredoxin reductase TR2; thioredoxin and
                     glutathione reductase; thioredoxin reductase 3 intronic
                     transcript 1; thioredoxin reductase 2 intronic transcript
                     1; thioredoxin reductase 3 new transcript 1; TXNRD3
                     neighbor gene protein; thioredoxin reductase 3 neighbor
                     gene protein; selenoprotein TXNRD3"
                     /codon_start=1
                     /transl_except=(pos:2058..2060,aa:Sec)
                     /product="thioredoxin reductase 3 isoform 1"
                     /protein_id="NP_443115.1"
                     /db_xref="CCDS:CCDS77811.1"
                     /db_xref="GeneID:114112"
                     /db_xref="HGNC:HGNC:20667"
                     /db_xref="MIM:606235"
                     /translation="MERSPPQSPGPGKAGDAPNRRSGHVRGARVLSPPGRRARLSSPG
                     PSRSSEAREELRRHLVGLIERSRVVIFSKSYCPHSTRVKELFSSLGVECNVLELDQVD
                     DGARVQEVLSEITNQKTVPNIFVNKVHVGGCDQTFQAYQSGLLQKLLQEDLAYDYDLI
                     IIGGGSGGLSCAKEAAILGKKVMVLDFVVPSPQGTSWGLGGTCVNVGCIPKKLMHQAA
                     LLGQALCDSRKFGWEYNQQVRHNWETMTKAIQNHISSLNWGYRLSLREKAVAYVNSYG
                     EFVEHHKIKATNKKGQETYYTAAQFVIATGERPRYLGIQGDKEYCITSDDLFSLPYCP
                     GKTLVVGASYVALECAGFLAGFGLDVTVMVRSILLRGFDQEMAEKVGSYMEQHGVKFL
                     RKFIPVMVQQLEKGSPGKLKVLAKSTEGTETIEGVYNTVLLAIGRDSCTRKIGLEKIG
                     VKINEKSGKIPVNDVEQTNVPYVYAVGDILEDKPELTPVAIQSGKLLAQRLFGASLEK
                     CDYINVPTTVFTPLEYGCCGLSEEKAIEVYKKENLEIYHTLFWPLEWTVAGRENNTCY
                     AKIICNKFDHDRVIGFHILGPNAGEVTQGFAAAMKCGLTKQLLDDTIGIHPTCGEVFT
                     TLEITKSSGLDITQKGCUG"
     exon            378..438
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            439..548
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            549..653
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            654..726
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            727..846
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            847..989
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1105
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1331
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1424
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1501
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1658
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1766
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1767..1862
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1863..1997
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2921
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2275..2351
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      2453..2458
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /note="hexamer: AATATA"
     polyA_site      2478
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /note="major polyA site"
     polyA_site      2534
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
     regulatory      2763..2768
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /note="hexamer: GATAAA"
     polyA_site      2789
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
     regulatory      2893..2898
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /note="hexamer: GATAAA"
     regulatory      2903..2908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
                     /note="hexamer: CATAAA"
     polyA_site      2912
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
     polyA_site      2921
                     /gene="TXNRD3"
                     /gene_synonym="TGR; TR2; TR2IT1; TRXR3; TXNR3; TXNRD3IT1;
                     TXNRD3NB; TXNRD3NT1"
ORIGIN      
        1 gcggattcgc gcgggtggtg agagtggcca gcgagggcag cgttcgccgc ccctcaggcc
       61 ccgtcccggc gccgcagccg cctgcgtttc ggtttgtgag caggcccggc cgggccagga
      121 gcgagagcga gactctggag cggtcgccgc cgcagtcgcc cgggccggga aaggcgggcg
      181 atgcccccaa ccgccgctcg ggccatgtcc gaggggcgcg cgtgttgtcg ccgccggggc
      241 gccgtgcccg cctgtcgtcc cccgggccca gccgctcgtc cgaggcccgc gaggagctgc
      301 gccgccacct cgtgggcctc atcgagcgca gccgggtggt gatcttcagc aagagctact
      361 gtccccatag tactcgggtg aaagaactct tttcttcttt gggagtcgaa tgtaatgtct
      421 tggaacttga tcaagttgat gatggggcca gggttcaaga agtgctgtca gaaatcacta
      481 atcagaaaac tgtgcccaat attttcgtga ataaagtgca tgtaggtgga tgtgaccaaa
      541 ctttccaggc atatcagagt ggtttgttac agaagctcct tcaggaagat ttggcatatg
      601 attatgatct catcatcatc ggtggtggtt ctggaggcct ttcatgtgcg aaggaagctg
      661 ccattttggg aaagaaagtt atggtgctag actttgttgt cccgtcacct cagggcacat
      721 cctggggtct tggtggcact tgtgtaaatg taggttgtat tcctaagaaa ttgatgcatc
      781 aggctgccct tttggggcag gcattatgtg actcaaggaa atttggctgg gaatataatc
      841 aacaagtgag gcacaactgg gagacaatga caaaagcgat tcagaaccac atcagctctc
      901 taaactgggg ctacaggttg tctctgaggg aaaaggctgt ggcctatgtc aattcctatg
      961 gagaatttgt tgaacatcat aaaataaagg caaccaataa aaaaggacag gagacttatt
     1021 atactgctgc acagtttgtc atagcaacgg gtgaaaggcc acggtattta ggaatccaag
     1081 gagataaaga atactgtatt actagtgatg accttttttc tctgccttat tgccctggca
     1141 aaacattagt ggtgggtgcc tcttatgttg ccctggagtg tgcagggttt ctggctggct
     1201 ttggcctaga tgtcacagtt atggtacgct caatccttct ccgtggcttc gaccaagaaa
     1261 tggcagaaaa agtgggttcc tacatggagc agcatggtgt gaagttccta cggaaattca
     1321 tacctgtgat ggttcaacag ttggagaaag gttcacctgg aaagctgaaa gtgttggcta
     1381 aatccactga aggaacagaa acaattgaag gagtctataa cacagttttg ttagctattg
     1441 gtcgtgactc ctgtacaagg aaaataggct tggagaagat tggtgtcaaa attaatgaga
     1501 agagtggaaa aatacctgta aatgatgtgg aacagaccaa tgtgccatat gtctatgctg
     1561 ttggtgatat tttggaggat aagccagagc tcactcctgt cgccatacag tcaggcaagc
     1621 tgctagctca gagacttttt ggggcctctt tagaaaagtg tgattatatt aatgttccga
     1681 ctacagtgtt tactcctctg gagtatggtt gctgtggatt atctgaagag aaagctattg
     1741 aagtatataa aaaagagaat ctagaaatat atcatacttt gttctggcct cttgaatgga
     1801 cagtagctgg cagagagaac aacacttgtt atgcaaagat aatctgcaat aaattcgacc
     1861 atgatcgggt gataggattt catattcttg gaccaaacgc cggtgaggtt acccaaggat
     1921 ttgcagctgc aatgaaatgt gggctcacaa aacagctact tgatgacacc attggaattc
     1981 accccacatg tggggaggtg ttcacgactt tggaaatcac aaagtcgtca ggactagaca
     2041 tcactcagaa aggctgctga ggctaggcct gctgctgttt agttctcctt gtcatattct
     2101 catttctctc aaagataaga atgctctcgg ataaaatgag cctgtgctca tgacagctgc
     2161 tctgttactc agggaccagt gcagggctgt cttacgacac ttagatgaga aagtagacaa
     2221 ggaaagagga cagcagtggg catctgcctt gtggtcttgc tgacagcgag aagcagtggg
     2281 actgcttcct tgacgcctta gcttggagcc ccgttatgag gtgagccaag gctgactctc
     2341 gcaagccagg actgagcttc cctcggaaag acctttgagt ggcaccattc acctaagtta
     2401 gcttttctgg tcgctattgt ttttatcccc tttgctttgt tgtttctgtg aaaatatatt
     2461 ttcagttaag aaatgctata aagtagtggt tttctagtgc gtgggcctag aattcacaca
     2521 tgctcactgg tgaacagtgt ctgtgggctg ctgtgggctg catttggata gctgtctgca
     2581 ttagcaggaa gctgtacaca ctgtgtaggg ttagtcatct cttttctttt ttgaggcaca
     2641 tttaacctac tgtcaaaacg ctgactcatg cttcaccctc acactcacta cttggtggtg
     2701 ataaccttga agctattctc ccctaattaa tatctcgaga ctgagcaaaa tatctcatgt
     2761 aagataaaag tacttgaatt gctttttcat gttcatctta atagtattat ttccaaaagt
     2821 ttttaatact gtattatgtg aaatctaact aatttatctg tgaaataatt ccatcacagt
     2881 ctgtttaatg atgataaagt tccataaatg aatacaggta a
//
