LOCUS       NM_002822               2987 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens twinfilin actin binding protein 1 (TWF1), transcript
            variant 2, mRNA.
ACCESSION   NM_002822 XM_936937 XM_943082
VERSION     NM_002822.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Wang,Y., Zuo,R., Huo,G., Han,Z., He,Y., Luo,Y., Chen,L., Li,G.,
            Cui,J., Zhu,F., Yue,P., Yuan,D., Sun,Y., Li,Z., Chen,P. and Guo,H.
  TITLE     TWF1 induces autophagy and accelerates malignant phenotype in lung
            adenocarcinoma via inhibiting the cAMP signaling pathway
  JOURNAL   FASEB J 37 (7), e23051 (2023)
   PUBMED   37358822
  REMARK    GeneRIF: TWF1 induces autophagy and accelerates malignant phenotype
            in lung adenocarcinoma via inhibiting the cAMP signaling pathway.
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Yang,Y., Shi,L., Zhang,D., Wu,D., An,Y., Zhang,Y. and Chen,X.
  TITLE     Long non-coding RNA FGD5-AS1 contributes to cisplatin resistance in
            hepatocellular carcinoma via sponging microRNA-153-3p by
            upregulating Twinfilin Actin Binding Protein 1 (TWF1)
  JOURNAL   Bioengineered 12 (1), 6713-6723 (2021)
   PUBMED   34519634
  REMARK    GeneRIF: Long non-coding RNA FGD5-AS1 contributes to cisplatin
            resistance in hepatocellular carcinoma via sponging microRNA-153-3p
            by upregulating Twinfilin Actin Binding Protein 1 (TWF1).
REFERENCE   3  (bases 1 to 2987)
  AUTHORS   Du,J., Shao,Y., Hu,Y., Chen,Y., Cang,J., Chen,X., Pei,W., Miao,F.,
            Shen,Y., Muddassir,M., Zhang,Y., Zhang,J. and Teng,G.
  TITLE     Multifunctional Liposomes Enable Active Targeting and Twinfilin 1
            Silencing to Reverse Paclitaxel Resistance in Brain Metastatic
            Breast Cancer
  JOURNAL   ACS Appl Mater Interfaces 13 (20), 23396-23409 (2021)
   PUBMED   33982563
  REMARK    GeneRIF: Multifunctional Liposomes Enable Active Targeting and
            Twinfilin 1 Silencing to Reverse Paclitaxel Resistance in Brain
            Metastatic Breast Cancer.
REFERENCE   4  (bases 1 to 2987)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2987)
  AUTHORS   Vartiainen,M.K., Sarkkinen,E.M., Matilainen,T., Salminen,M. and
            Lappalainen,P.
  TITLE     Mammals have two twinfilin isoforms whose subcellular localizations
            and tissue distributions are differentially regulated
  JOURNAL   J Biol Chem 278 (36), 34347-34355 (2003)
   PUBMED   12807912
  REMARK    GeneRIF: mammals have two twinfilin isoforms, which are
            differentially expressed and regulated through distinct cellular
            signaling pathways
REFERENCE   6  (bases 1 to 2987)
  AUTHORS   Paavilainen,V.O., Merckel,M.C., Falck,S., Ojala,P.J., Pohl,E.,
            Wilmanns,M. and Lappalainen,P.
  TITLE     Structural conservation between the actin monomer-binding sites of
            twinfilin and actin-depolymerizing factor (ADF)/cofilin
  JOURNAL   J Biol Chem 277 (45), 43089-43095 (2002)
   PUBMED   12207032
REFERENCE   7  (bases 1 to 2987)
  AUTHORS   Ojala,P.J., Paavilainen,V.O., Vartiainen,M.K., Tuma,R., Weeds,A.G.
            and Lappalainen,P.
  TITLE     The two ADF-H domains of twinfilin play functionally distinct roles
            in interactions with actin monomers
  JOURNAL   Mol Biol Cell 13 (11), 3811-3821 (2002)
   PUBMED   12429826
  REMARK    GeneRIF: These data suggest that the association with an actin
            monomer induces a first-order conformational change within the
            twinfilin molecule.
REFERENCE   8  (bases 1 to 2987)
  AUTHORS   Palmgren,S., Vartiainen,M. and Lappalainen,P.
  TITLE     Twinfilin, a molecular mailman for actin monomers
  JOURNAL   J Cell Sci 115 (Pt 5), 881-886 (2002)
   PUBMED   11870207
  REMARK    Review article
REFERENCE   9  (bases 1 to 2987)
  AUTHORS   Rohwer,A., Kittstein,W., Marks,F. and Gschwendt,M.
  TITLE     Cloning, expression and characterization of an A6-related protein
  JOURNAL   Eur J Biochem 263 (2), 518-525 (1999)
   PUBMED   10406962
REFERENCE   10 (bases 1 to 2987)
  AUTHORS   Beeler,J.F., LaRochelle,W.J., Chedid,M., Tronick,S.R. and
            Aaronson,S.A.
  TITLE     Prokaryotic expression cloning of a novel human tyrosine kinase
  JOURNAL   Mol Cell Biol 14 (2), 982-988 (1994)
   PUBMED   7507208
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC043148.1.
            
            On Jun 3, 2019 this sequence version replaced NM_002822.4.
            
            Summary: This gene encodes twinfilin, an actin monomer-binding
            protein conserved from yeast to mammals. Studies of the mouse
            counterpart suggest that this protein may be an actin
            monomer-binding protein, and its localization to cortical
            G-actin-rich structures may be regulated by the small GTPase RAC1.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            coding region, compared to variant 1. The encoded isoform (2) is
            shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC043148.1, U02680.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000395510.7/ ENSP00000378886.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2987              BC043148.1         62-3048
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q12"
     gene            1..2987
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /note="twinfilin actin binding protein 1"
                     /db_xref="GeneID:5756"
                     /db_xref="HGNC:HGNC:9620"
                     /db_xref="MIM:610932"
     exon            1..97
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..1125
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /note="isoform 2 is encoded by transcript variant 2; PTK9
                     protein tyrosine kinase 9; A6 protein tyrosine kinase;
                     twinfilin-1; protein A6; twinfilin, actin-binding protein,
                     homolog 1"
                     /codon_start=1
                     /product="twinfilin-1 isoform 2"
                     /protein_id="NP_002813.3"
                     /db_xref="CCDS:CCDS31780.2"
                     /db_xref="GeneID:5756"
                     /db_xref="HGNC:HGNC:9620"
                     /db_xref="MIM:610932"
                     /translation="MSHQTGIQASEDVKEIFARARNGKYRLLKISIENEQLVIGSYSQ
                     PSDSWDKDYDSFVLPLLEDKQPCYILFRLDSQNAQGYEWIFIAWSPDHSHVRQKMLYA
                     ATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKIN
                     EVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTT
                     NTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCK
                     SRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQHAHKQSFAKPKGPAGKRG
                     IRRLIRGPAETEATTD"
     exon            98..175
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /inference="alignment:Splign:2.1.0"
     exon            176..354
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /inference="alignment:Splign:2.1.0"
     exon            355..450
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /inference="alignment:Splign:2.1.0"
     exon            451..555
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /inference="alignment:Splign:2.1.0"
     exon            556..681
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /inference="alignment:Splign:2.1.0"
     exon            682..832
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /inference="alignment:Splign:2.1.0"
     exon            833..954
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /inference="alignment:Splign:2.1.0"
     exon            955..2987
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2966..2971
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /note="hexamer: AATAAA"
     polyA_site      2987
                     /gene="TWF1"
                     /gene_synonym="A6; PTK9"
                     /note="major polyA site"
ORIGIN      
        1 cacttcctgg ggccgccggc cggggccgct ggctgcactc agcgccggag ccgggagcta
       61 gcggccgccg ccatgtccca ccagaccggc atccaagcaa gtgaagatgt taaagagatc
      121 tttgccagag ccagaaatgg aaagtacaga cttctgaaaa tatctattga aaatgagcaa
      181 cttgtgattg gatcatatag tcagccttca gattcctggg ataaggatta tgattccttt
      241 gttttacccc tgttggagga caaacaacca tgctatatat tattcaggtt agattctcag
      301 aatgcccagg gatatgaatg gatattcatt gcatggtctc cagatcattc tcatgttcgt
      361 caaaaaatgt tgtatgcagc aacaagagca actctgaaga aggaatttgg aggtggccac
      421 attaaagatg aagtatttgg aacagtaaag gaagatgtat cattacatgg atataaaaaa
      481 tacttgctgt cacaatcttc ccctgcccca ctgactgcag ctgaggaaga actacgacag
      541 attaaaatca atgaggtaca gactgacgtg ggtgtggaca ctaagcatca aacactacaa
      601 ggagtagcat ttcccatttc tcgagaagcc tttcaggctt tggaaaaatt gaataataga
      661 cagctcaact atgtgcagtt ggaaatagat ataaaaaatg aaattataat tttggccaac
      721 acaacaaata cagaactgaa agatttgcca aagaggattc ccaaggattc agctcgttac
      781 catttctttc tgtataaaca ttcccatgaa ggagactatt tagagtccat agtttttatt
      841 tattcaatgc ctggatacac atgcagtata agagagcgga tgctgtattc tagctgcaag
      901 agccgtctgc tagaaattgt agaaagacaa ctacaaatgg atgtaattag aaagatcgag
      961 atagacaatg gggatgagtt gactgcagac ttcctttatg aagaagtaca tcccaagcag
     1021 catgcacaca agcaaagttt tgcaaaacca aaaggtcctg caggaaaaag aggaattcga
     1081 agactaatta ggggcccagc ggaaactgaa gctactactg attaaagtca tcacattaaa
     1141 cattgtaata ctagtttttt aaaagtccag cttttagtac aggagaactg aaatcattcc
     1201 atgttgatat aaagtaggga aaaaaattgt actttttgga aaatagcact tttcacttct
     1261 gtgtgttttt aaaattaatg ttatagaaga ctcatgattt ctatttttga gttaaagcta
     1321 gaaaagggtt caacataatg tttaattttg tcacactgtt ttcatagcgt tgattccaca
     1381 cttcaaatac ttcttaaaat tttatacagt tgggccagtt ctagaaagtc tgatgtctca
     1441 aagggtaaac ttactacttt cttgtgggac agaaagacct taaaatattc atattactta
     1501 atgaatatgt taaggaccag gctagagtat tttctaagct ggaaacttag tgtgccttgg
     1561 aaaaggccgc aagttgctta ctccgagtag ctgtgctagc tctgtcagac tgtaggatca
     1621 tgtctgcaac ttttagaaat agtgctttat attgcagcag tcttttatat ttgacttttt
     1681 tttaatagca ttaaaattgc agatcagctc actctgaaac tttaagggta ccagatattt
     1741 tctatactgc aggatttctg atgacattga aagactttaa acagccttag taaattatct
     1801 ttctaatgct ctgtgaggcc aaacatttat gttcagattg aaatttaaat taatatcatt
     1861 caaaaggaaa caaaaaatgt tgagttttaa aaatcaggat tgactttttt ctccaaaacc
     1921 atacatttat gggcaaattg tgttctttat cacttccgag caaatactca gatttaaaat
     1981 tactttaaag tcctggtact taacaggcta acgtagataa acaccttaat aatctcagtt
     2041 aatactgtat ttcaaaacac atttaactgt tttctaatgc tttgcattat cagttacaac
     2101 ctagagagat tttgagcctc atatttcttt gatacttgaa atagagggag ctagaacact
     2161 taatgtttaa tctgttaaac ctgctgcaag agccataact ttgaggcatt ttctaaatga
     2221 actgtgggga tccaggattt gtaatttctt gatctaaact ttatgctgca taaatcactt
     2281 atcggaaatg cacatttcat agtgtgaagc actcatttct aaaccttatt atctaaggta
     2341 atatatgcac ctttcagaaa tttgtgttcg agtaagtaaa gcatattaga ataattgtgg
     2401 gttgacagat ttttaaaata gaatttagag tatttggggt tttgtttgtt tacaaataat
     2461 cagactataa tatttaaaca tgcaaaataa ctgacaataa tgttgcactt gtttactaaa
     2521 gatataagtt gttccatggg tgtacacgta gacagacaca catacaccca aattattgca
     2581 ttaagaatcc tggagcagac catagctgaa gctgttattt tcagtcagga agactacctg
     2641 tcatgaaggt ataaaataat ttagaagtga atgtttttct gtaccatcta tgtgcaatta
     2701 tactctaaat tccactacac tacattaaag taaatggaca ttccagaata tagatgtgat
     2761 tatagtctta aactaattat tattaaacca atgattgctg aaaatcagtg atgcatttgt
     2821 tatagagtat aactcatcgt ttacagtatg ttttagttgg cagtatcata cctagatggt
     2881 gaataacata ttcccagtaa atttatatag cagtgaagaa ttacatgcct tctggtggac
     2941 attttataag tgcattttat atcacaataa aaattttttc tctttaa
//
