LOCUS       NM_015269               5856 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens terminal uridylyl transferase 4 (TUT4), transcript
            variant 2, mRNA.
ACCESSION   NM_015269
VERSION     NM_015269.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Zhang,P., Frederick,M.I. and Heinemann,I.U.
  TITLE     Terminal Uridylyltransferases TUT4/7 Regulate microRNA and mRNA
            Homeostasis
  JOURNAL   Cells 11 (23), 3742 (2022)
   PUBMED   36497000
  REMARK    GeneRIF: Terminal Uridylyltransferases TUT4/7 Regulate microRNA and
            mRNA Homeostasis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Yang,A., Bofill-De Ros,X., Stanton,R., Shao,T.J., Villanueva,P. and
            Gu,S.
  TITLE     TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and
            abundance
  JOURNAL   Nat Commun 13 (1), 5260 (2022)
   PUBMED   36071058
  REMARK    GeneRIF: TENT2, TUT4, and TUT7 selectively regulate miRNA sequence
            and abundance.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5856)
  AUTHORS   Stein,R.L. and Wilson,D.M.
  TITLE     Kinetic and Mechanistic Studies of the Terminal
            Uridylyltransferase, Zcchc11 (TUT4)
  JOURNAL   Biochemistry 61 (15), 1614-1624 (2022)
   PUBMED   35797480
REFERENCE   4  (bases 1 to 5856)
  AUTHORS   Medhi,R., Price,J., Furlan,G., Gorges,B., Sapetschnig,A. and
            Miska,E.A.
  TITLE     RNA uridyl transferases TUT4/7 differentially regulate miRNA
            variants depending on the cancer cell type
  JOURNAL   RNA 28 (3), 353-370 (2022)
   PUBMED   34949722
  REMARK    GeneRIF: RNA uridyl transferases TUT4/7 differentially regulate
            miRNA variants depending on the cancer cell type.
REFERENCE   5  (bases 1 to 5856)
  AUTHORS   Gaza,A., Fritz,V., Malek,L., Wormser,L., Treiber,N., Danner,J.,
            Kremer,A.E., Thasler,W.E., Siebler,J., Meister,G., Neurath,M.F.,
            Hellerbrand,C., Bosserhoff,A.K. and Dietrich,P.
  TITLE     Identification of novel targets of miR-622 in hepatocellular
            carcinoma reveals common regulation of cooperating genes and
            outlines the oncogenic role of zinc finger CCHC-type containing 11
  JOURNAL   Neoplasia 23 (5), 502-514 (2021)
   PUBMED   33901943
  REMARK    GeneRIF: Identification of novel targets of miR-622 in
            hepatocellular carcinoma reveals common regulation of cooperating
            genes and outlines the oncogenic role of zinc finger CCHC-type
            containing 11.
REFERENCE   6  (bases 1 to 5856)
  AUTHORS   Jones,M.R., Quinton,L.J., Blahna,M.T., Neilson,J.R., Fu,S.,
            Ivanov,A.R., Wolf,D.A. and Mizgerd,J.P.
  TITLE     Zcchc11-dependent uridylation of microRNA directs cytokine
            expression
  JOURNAL   Nat Cell Biol 11 (9), 1157-1163 (2009)
   PUBMED   19701194
  REMARK    GeneRIF: Zcchc11 fine tunes IL-6 production by uridylating miR-26a.
REFERENCE   7  (bases 1 to 5856)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   8  (bases 1 to 5856)
  AUTHORS   Mullen,T.E. and Marzluff,W.F.
  TITLE     Degradation of histone mRNA requires oligouridylation followed by
            decapping and simultaneous degradation of the mRNA both 5' to 3'
            and 3' to 5'
  JOURNAL   Genes Dev 22 (1), 50-65 (2008)
   PUBMED   18172165
REFERENCE   9  (bases 1 to 5856)
  AUTHORS   Minoda,Y., Saeki,K., Aki,D., Takaki,H., Sanada,T., Koga,K.,
            Kobayashi,T., Takaesu,G. and Yoshimura,A.
  TITLE     A novel Zinc finger protein, ZCCHC11, interacts with TIFA and
            modulates TLR signaling
  JOURNAL   Biochem Biophys Res Commun 344 (3), 1023-1030 (2006)
   PUBMED   16643855
  REMARK    GeneRIF: We propose that ZCCHC11 is a unique TLR signal regulator,
            which interacts with TIFA after LPS treatment and suppresses the
            TRAF6-dependent activation of NF-kappaB.
            Erratum:[Biochem Biophys Res Commun. 2007 Feb 23;353(4):1121]
REFERENCE   10 (bases 1 to 5856)
  AUTHORS   Keller,W. and Martin,G.
  TITLE     Gene regulation: reviving the message
  JOURNAL   Nature 419 (6904), 267-268 (2002)
   PUBMED   12239557
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA748708.1, BC035820.1, D83776.1 and R60512.1.
            
            On Jun 19, 2010 this sequence version replaced NM_015269.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site at the 5' end of a coding exon compared to variant 1.
            The resulting isoform (b) has the same N- and C-termini but is
            shorter compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.213454.1,
                                           SRR1803612.148063.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                DA748708.1         32-50
            20-635              BC035820.1         12-627
            636-5838            D83776.1           1-5203
            5839-5856           R60512.1           1-18                c
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p32.3"
     gene            1..5856
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /note="terminal uridylyl transferase 4"
                     /db_xref="GeneID:23318"
                     /db_xref="HGNC:HGNC:28981"
                     /db_xref="MIM:613692"
     exon            1..160
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            161..971
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     CDS             254..5188
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /EC_number="2.7.7.52"
                     /note="isoform b is encoded by transcript variant 2; PAP
                     associated domain containing 3; zinc finger CCHC-type
                     containing 11; terminal uridylyltransferase 4; TUTase 4;
                     zinc finger CCHC domain-containing protein 11; zinc
                     finger, CCHC domain containing 11"
                     /codon_start=1
                     /product="terminal uridylyltransferase 4 isoform b"
                     /protein_id="NP_056084.1"
                     /db_xref="CCDS:CCDS30716.1"
                     /db_xref="GeneID:23318"
                     /db_xref="HGNC:HGNC:28981"
                     /db_xref="MIM:613692"
                     /translation="MEESKTLKSENHEPKKNVICEESKAVQVIGNQTLKARNDKSVKE
                     IENSSPNRNSSKKNKQNDICIEKTEVKSCKVNAANLPGPKDLGLVLRDQSHCKAKKFP
                     NSPVKAEKATISQAKSEKATSLQAKAEKSPKSPNSVKAEKASSYQMKSEKVPSSPAEA
                     EKGPSLLLKDMRQKTELQQIGKKIPSSFTSVDKVNIEAVGGEKCALQNSPRSQKQQTC
                     TDNTGDSDDSASGIEDVSDDLSKMKNDESNKENSSEMDYLENATVIDESALTPEQRLG
                     LKQAEERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKKNILE
                     KQEESELRSLPPPSPAHLAALSVAVIELAKEHGITDDDLRVRQEIVEEMSKVITTFLP
                     ECSLRLYGSSLTRFALKSSDVNIDIKFPPKMNHPDLLIKVLGILKKNVLYVDVESDFH
                     AKVPVVVCRDRKSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCY
                     IDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEK
                     FVKWECNSSSATEKNSIAEENKAKADQPKDDTKKTETDNQSNAMKEKHGKSPLALETP
                     NRVSLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRN
                     VARSLNSQLVYEYVVERFRAAYRYFACPQTKGGNKSTVDFKKREKGKISNKKPVKSNN
                     MATNGCILLGETTEKINAEREQPVQCDEMDCTSQRCIIDNNNLLVNELDFADHGQDSS
                     SLSTSKSSEIEPKLDKKQDDLAPSETCLKKELSQCNCIDLSKSPDPDKSTGTDCRSNL
                     ETESSHQSVCTDTSATSCNCKATEDASDLNDDDNLPTQELYYVFDKFILTSGKPPTIV
                     CSICKKDGHSKNDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDELSPPCSEQHNR
                     EQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDICMTLEGHENAEKLNCKEI
                     IENLAKILKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDISLYNTLAQHNTRMLATY
                     AAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEI
                     FDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKE
                     YVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLF
                     GTPFYPLIGREAEYFFDSRVLTDGELAPNDRCCRVCGKIGHYMKDCPKRKSLLFRLKK
                     KDSEEEKEGNEEEKDSRDVLDPRDLHDTRDFRDPRDLRCFICGDAGHVRRECPEVKLA
                     RQRNSSVAAAQLVRNLVNAQQVAGSAQQQGDQSIRTRQSSECSESPSYSPQPQPFPQN
                     SSQSAAITQPSSQPGSQPKLGPPQQGAQPPHQVQMPLYNFPQSPPAQYSPMHNMGLLP
                     MHPLQIPAPSWPIHGPVIHSAPGSAPSNIGLNDPSIIFAQPAARPVAIPNTSHDGHWP
                     RTVAPNSLVNSGAVGNSEPGFRGLTPPIPWEHAPRPHFPLVPASWPYGLHQNFMHQGN
                     ARFQPNKPFYTQDRCATRRCRERCPHPPRGNVSE"
     exon            972..1135
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1252
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1430
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1519
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1571
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1641
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1768
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1769..1888
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..2101
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..2276
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2980
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            2981..3131
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3132..3218
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3219..3322
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3323..3380
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3381..3484
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3485..3574
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3575..3688
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3689..3766
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3767..3944
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3945..3992
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3993..4075
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            4076..4191
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            4192..4412
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            4413..4513
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            4514..4961
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            4962..5120
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            5121..5848
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5705..5710
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /note="hexamer: AATAAA"
     polyA_site      5723
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /note="major polyA site"
     regulatory      5823..5828
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /note="hexamer: AATAAA"
     polyA_site      5848
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /note="major polyA site"
ORIGIN      
        1 ttcgcggcgc ggcggacatg gcggtggagt cctgagctag tcgtgtcccg gcctccagcg
       61 gcggcagcgg cgacggcggc ggcagcagga ggcggaggga gagcaggagc cactgagatg
      121 aggaggcgtc gcgcgcgcct gtagctcgcg ggcctcgcag tttgcttgcc taaactggac
      181 ttgaagcaat ttaaactact ggagatctgc cattttataa tatcacaatt ggaaagaaac
      241 agattttcaa ataatggaag agtctaaaac cttaaaaagt gaaaatcatg aaccaaagaa
      301 gaatgttatt tgtgaagaaa gcaaagcagt tcaagttata ggtaatcaaa cattgaaagc
      361 tagaaatgat aaatccgtaa aagaaattga gaacagctct ccaaatagga atagtagtaa
      421 aaaaaataag caaaatgata tttgtataga aaaaacagaa gttaaatcat gtaaagtaaa
      481 tgctgccaac cttccaggtc ctaaagattt ggggttagtc cttcgagatc aaagtcattg
      541 caaagcaaaa aaatttccta attcaccggt gaaagccgaa aaggcaacca tttcacaggc
      601 aaaatcagaa aaggcaacca gtttacaggc aaaagcagaa aaatcaccaa agtcacctaa
      661 ttcagtgaaa gcagaaaaag catccagtta tcagatgaag tcagaaaaag taccaagttc
      721 accagcagaa gcagaaaagg gacccagttt actgttgaaa gacatgagac agaagacaga
      781 attacaacag attggaaaaa aaattccaag ctcctttact tctgtggaca aagtgaatat
      841 tgaagctgta gggggagaaa aatgtgctct gcaaaactca ccacgatctc agaagcaaca
      901 gacatgtaca gataatactg gtgattctga tgatagtgct tcaggaattg aagacgtatc
      961 ggacgattta agtaaaatga agaatgatga atctaataaa gaaaattctt cagagatgga
     1021 ctacttagaa aatgccactg tgatagatga atctgcattg acacctgagc agaggctggg
     1081 gttgaaacaa gcagaagaac gcttagaaag agatcacatc tttcgacttg aaaaacgatc
     1141 accagaatat accaattgtc ggtatctatg caaactttgc ttaattcaca ttgaaaatat
     1201 ccagggggct cataaacata taaaggagaa acgacataag aaaaatattt tggaaaaaca
     1261 agaagaaagt gagcttcgtt ctctgccacc tccttcccct gcccacttgg ctgctttaag
     1321 tgttgcagtc attgaattag caaaagaaca tggaataaca gatgatgacc tcagagtccg
     1381 tcaggaaatt gtggaggaaa tgtcaaaggt tataacgaca tttttaccag aatgttcact
     1441 taggttgtat ggctcatctc tgactagatt tgctctgaaa agtagtgatg ttaatataga
     1501 tataaaattt cctcccaaga tgaatcatcc agatcttctg ataaaagtac ttgggatttt
     1561 aaagaaaaat gtattatatg tagatgtgga atctgatttt cacgctaaag ttcctgttgt
     1621 ggtgtgcaga gatcgaaaaa gtggtttact ttgtagagtg agtgcaggaa acgatatggc
     1681 atgtctcact actgatttac ttactgccct tggcaaaata gaacctgtct ttattccctt
     1741 ggtgttagcc tttcgctact gggctaagtt gtgctatatt gactcccaaa ctgatggtgg
     1801 aatcccttct tactgttttg ctttaatggt gatgtttttt ctacaacaga gaaaaccccc
     1861 tcttcttcct tgcttacttg gaagttggat tgaaggcttt gacccaaaaa gaatggatga
     1921 ctttcagctg aagggcatag tagaagagaa gtttgtgaag tgggaatgta attcaagtag
     1981 tgcaactgag aaaaactcaa ttgctgagga aaacaaagct aaggcagacc aaccaaaaga
     2041 tgataccaag aagacagaaa cagacaacca aagtaatgcc atgaaggaaa aacatggcaa
     2101 atctccttta gcattggaaa caccaaatcg ggtatccttg ggacagttat ggttagagct
     2161 gcttaaattc tacacactgg attttgcttt ggaggaatat gtcatatgtg tacggataca
     2221 agatatttta acaagagaaa ataaaaactg gcctaaaagg cgaatagcca ttgaagatcc
     2281 attttcagtc aagaggaatg ttgcacggag cttaaacagc cagctggttt acgaatatgt
     2341 tgtggagaga tttagggcag cttatcggta ttttgcctgt cctcagacga agggtggaaa
     2401 taagtctaca gtggatttca agaaaagaga gaaggggaaa ataagcaata agaaaccagt
     2461 caagtcgaac aatatggcaa ccaatggttg tattctgctt ggggaaacca cagaaaaaat
     2521 aaatgcagaa agagagcaac ctgttcaatg tgatgaaatg gactgtacat cacagagatg
     2581 tattattgac aacaacaatt tgttggtaaa tgaactagat tttgctgacc acggacagga
     2641 ctcttcatct ctttctacca gcaaaagcag tgaaatagag ccaaaattag ataagaaaca
     2701 agatgattta gcgccttcag aaacttgttt aaaaaaagag ctcagccaat gtaattgcat
     2761 tgatttgtct aagtcgcctg acccagataa atctactgga acagactgca ggtcaaattt
     2821 agaaacagag agttcacatc agagtgtgtg caccgacaca tctgctacct cttgcaactg
     2881 caaagctaca gaagatgctt ctgaccttaa tgatgatgat aacctcccca cccaggaatt
     2941 atattatgtg tttgataagt ttattttaac ctctggcaag ccaccaacga tagtatgcag
     3001 catctgcaaa aaggatggcc attcaaagaa tgattgccca gaggatttta ggaaaattga
     3061 tttaaaacct ctaccaccaa tgacaaaccg atttcgggaa atacttgatt tagtatgtaa
     3121 aagatgtttt gatgagttat caccaccttg ttctgaacaa cacaacaggg agcaaatttt
     3181 aattggcttg gaaaagttta ttcaaaaaga atatgatgaa aaggcaaggt tgtgcttatt
     3241 tggctcttct aagaatggat ttggatttcg tgatagtgat ctggatattt gtatgaccct
     3301 ggaaggccat gaaaatgcag agaaattaaa ttgtaaggaa ataattgaaa atttggcaaa
     3361 aattcttaag agacatccag gtttaagaaa cattttgcct ataactactg ccaaagtgcc
     3421 tatagtaaaa tttgaacaca ggcgaagtgg gttagaaggc gatatcagtt tatataatac
     3481 gttggctcaa cataacacaa gaatgctagc tacttatgca gctattgatc ctagagtgca
     3541 gtatttggga tatactatga aagtgtttgc taagcgatgt gacattgggg atgcttccag
     3601 gggaagttta tcttcatatg catatatcct tatggtgctg tactttctgc agcagagaaa
     3661 gccacctgtt atcccagttc tacaagagat ctttgatgga aaacagattc cacagagaat
     3721 ggttgatgga tggaatgctt tcttctttga taaaacagaa gaactgaaaa agcgtttacc
     3781 ttcacttgga aagaacacag aatcattagg ggagctttgg ctgggactgc ttcgtttcta
     3841 tactgaagag tttgatttca aggaatatgt aattagcatt cggcagaaaa agctgttgac
     3901 aacttttgag aagcagtgga cttccaagtg cattgcaatt gaagaccctt ttgacttgaa
     3961 tcataacctt ggtgctggag tttccagaaa aatgaccaat ttcatcatga aagcatttat
     4021 caatggaagg aaactttttg gtaccccttt ttatccactc attggcagag aagctgagta
     4081 cttctttgat tccagagtat taacagatgg agaactggct cccaatgata gatgttgccg
     4141 tgtgtgtgga aaaataggcc actacatgaa agactgccct aaaaggaaaa gtttactgtt
     4201 tagactaaag aagaaagaca gtgaagaaga gaaggaaggg aatgaagaag agaaagattc
     4261 ccgagatgtt cttgaccccc gagacctcca cgatactcga gactttagag acccgagaga
     4321 cctcagatgt tttatatgtg gagatgctgg acatgtacga agggagtgcc cagaggtcaa
     4381 gctggcccgt cagaggaata gcagtgtggc agcagcccag ctggtccgca accttgtaaa
     4441 tgctcaacag gtggctggtt cagctcagca acagggtgat cagtccataa ggactagaca
     4501 gtcatcagaa tgttctgaat caccatctta ttctcctcag cctcagccat ttccacagaa
     4561 ctcttcccag tcagctgcta ttactcagcc ttcatctcag ccaggatccc aacctaagct
     4621 tggcccacct cagcagggag cccaacctcc ccatcaggtc cagatgccac tgtataactt
     4681 tccccagtca ccaccagctc agtattctcc catgcacaat atgggattgt tgccaatgca
     4741 ccctctccag atccctgccc cgtcctggcc catccatggc ccagtgatcc actctgcacc
     4801 aggcagtgcc cccagcaata ttggcctaaa tgatcccagc atcatctttg cacagcctgc
     4861 tgccagacct gtggcaatcc ctaacacgtc tcacgatgga cactggcccc gtactgtggc
     4921 tccaaattcc ctggtaaaca gtggtgcagt ggggaattca gagccaggct ttcgaggact
     4981 gactcctcca attccttggg aacatgcacc gcgtccccat ttcccccttg tcccagcttc
     5041 gtggccttat ggtttgcatc aaaacttcat gcatcaggga aatgcccgat tccagcccaa
     5101 caaacctttc tatactcaag acagatgtgc cacccgtcgg tgtagagagc gttgtcccca
     5161 cccaccaaga ggaaacgtgt cggagtaatg cgagtccatt ttctttcagc tggtctaccg
     5221 atgcacagca accagccaat cctgctgtct caagggtatc cgtacctcaa tgtcagttac
     5281 attcagcaga aaaagtgaca tttaatggaa agcaaaacaa atcaggtcct gatttaaaat
     5341 tttaacacgt ttagatagct tatttaaatt ctaagactgt ttttacagac tgtattattt
     5401 gtatataaat atgaactata gtttttacta gtatcaccaa attgagtgat acaaatttca
     5461 tctattttat taccctattt ttaagggaat tttatgtttt gtttaacctt gatgaattgt
     5521 ataaagagag aatttaaaaa attacattct ttattttcta ttagctgtat tcatactttg
     5581 gttgcttcag actttatata ttgttcttaa aagttaggaa agaatttcat gtgttttaaa
     5641 ttgtcacttc tattctttac agaaccttgt agtgccaaaa actttttaaa aggtaaaaaa
     5701 ataaaataaa attacaacat gaagattcag attttttttc ttttccatac ttgtatattg
     5761 aacattcaaa cttgaatgga aagttgattt tgtctgtatt tgaagtactt ctattttaag
     5821 ttaataaatc tttttgacaa gagttaaaaa aaaaaa
//
