LOCUS       NM_001009881            5853 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens terminal uridylyl transferase 4 (TUT4), transcript
            variant 1, mRNA.
ACCESSION   NM_001009881
VERSION     NM_001009881.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Zhang,P., Frederick,M.I. and Heinemann,I.U.
  TITLE     Terminal Uridylyltransferases TUT4/7 Regulate microRNA and mRNA
            Homeostasis
  JOURNAL   Cells 11 (23), 3742 (2022)
   PUBMED   36497000
  REMARK    GeneRIF: Terminal Uridylyltransferases TUT4/7 Regulate microRNA and
            mRNA Homeostasis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Yang,A., Bofill-De Ros,X., Stanton,R., Shao,T.J., Villanueva,P. and
            Gu,S.
  TITLE     TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and
            abundance
  JOURNAL   Nat Commun 13 (1), 5260 (2022)
   PUBMED   36071058
  REMARK    GeneRIF: TENT2, TUT4, and TUT7 selectively regulate miRNA sequence
            and abundance.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5853)
  AUTHORS   Stein,R.L. and Wilson,D.M.
  TITLE     Kinetic and Mechanistic Studies of the Terminal
            Uridylyltransferase, Zcchc11 (TUT4)
  JOURNAL   Biochemistry 61 (15), 1614-1624 (2022)
   PUBMED   35797480
REFERENCE   4  (bases 1 to 5853)
  AUTHORS   Medhi,R., Price,J., Furlan,G., Gorges,B., Sapetschnig,A. and
            Miska,E.A.
  TITLE     RNA uridyl transferases TUT4/7 differentially regulate miRNA
            variants depending on the cancer cell type
  JOURNAL   RNA 28 (3), 353-370 (2022)
   PUBMED   34949722
  REMARK    GeneRIF: RNA uridyl transferases TUT4/7 differentially regulate
            miRNA variants depending on the cancer cell type.
REFERENCE   5  (bases 1 to 5853)
  AUTHORS   Gaza,A., Fritz,V., Malek,L., Wormser,L., Treiber,N., Danner,J.,
            Kremer,A.E., Thasler,W.E., Siebler,J., Meister,G., Neurath,M.F.,
            Hellerbrand,C., Bosserhoff,A.K. and Dietrich,P.
  TITLE     Identification of novel targets of miR-622 in hepatocellular
            carcinoma reveals common regulation of cooperating genes and
            outlines the oncogenic role of zinc finger CCHC-type containing 11
  JOURNAL   Neoplasia 23 (5), 502-514 (2021)
   PUBMED   33901943
  REMARK    GeneRIF: Identification of novel targets of miR-622 in
            hepatocellular carcinoma reveals common regulation of cooperating
            genes and outlines the oncogenic role of zinc finger CCHC-type
            containing 11.
REFERENCE   6  (bases 1 to 5853)
  AUTHORS   Jones,M.R., Quinton,L.J., Blahna,M.T., Neilson,J.R., Fu,S.,
            Ivanov,A.R., Wolf,D.A. and Mizgerd,J.P.
  TITLE     Zcchc11-dependent uridylation of microRNA directs cytokine
            expression
  JOURNAL   Nat Cell Biol 11 (9), 1157-1163 (2009)
   PUBMED   19701194
  REMARK    GeneRIF: Zcchc11 fine tunes IL-6 production by uridylating miR-26a.
REFERENCE   7  (bases 1 to 5853)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   8  (bases 1 to 5853)
  AUTHORS   Mullen,T.E. and Marzluff,W.F.
  TITLE     Degradation of histone mRNA requires oligouridylation followed by
            decapping and simultaneous degradation of the mRNA both 5' to 3'
            and 3' to 5'
  JOURNAL   Genes Dev 22 (1), 50-65 (2008)
   PUBMED   18172165
REFERENCE   9  (bases 1 to 5853)
  AUTHORS   Minoda,Y., Saeki,K., Aki,D., Takaki,H., Sanada,T., Koga,K.,
            Kobayashi,T., Takaesu,G. and Yoshimura,A.
  TITLE     A novel Zinc finger protein, ZCCHC11, interacts with TIFA and
            modulates TLR signaling
  JOURNAL   Biochem Biophys Res Commun 344 (3), 1023-1030 (2006)
   PUBMED   16643855
  REMARK    GeneRIF: We propose that ZCCHC11 is a unique TLR signal regulator,
            which interacts with TIFA after LPS treatment and suppresses the
            TRAF6-dependent activation of NF-kappaB.
            Erratum:[Biochem Biophys Res Commun. 2007 Feb 23;353(4):1121]
REFERENCE   10 (bases 1 to 5853)
  AUTHORS   Keller,W. and Martin,G.
  TITLE     Gene regulation: reviving the message
  JOURNAL   Nature 419 (6904), 267-268 (2002)
   PUBMED   12239557
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591167.6, DA748708.1, BC131734.1, AL138849.12 and AK025698.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001009881.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.215775.1,
                                           SRR1803614.237487.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000257177.9/ ENSP00000257177.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AL591167.6         13259-13260         c
            3-110               DA748708.1         32-139
            111-569             BC131734.1         1-459
            570-570             AL138849.12        111140-111140       c
            571-5252            BC131734.1         461-5142
            5253-5853           AK025698.1         1905-2505
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p32.3"
     gene            1..5853
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /note="terminal uridylyl transferase 4"
                     /db_xref="GeneID:23318"
                     /db_xref="HGNC:HGNC:28981"
                     /db_xref="MIM:613692"
     exon            1..162
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            163..973
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     CDS             256..5193
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /EC_number="2.7.7.52"
                     /note="isoform a is encoded by transcript variant 1; PAP
                     associated domain containing 3; zinc finger CCHC-type
                     containing 11; terminal uridylyltransferase 4; TUTase 4;
                     zinc finger CCHC domain-containing protein 11; zinc
                     finger, CCHC domain containing 11"
                     /codon_start=1
                     /product="terminal uridylyltransferase 4 isoform a"
                     /protein_id="NP_001009881.1"
                     /db_xref="CCDS:CCDS30715.1"
                     /db_xref="GeneID:23318"
                     /db_xref="HGNC:HGNC:28981"
                     /db_xref="MIM:613692"
                     /translation="MEESKTLKSENHEPKKNVICEESKAVQVIGNQTLKARNDKSVKE
                     IENSSPNRNSSKKNKQNDICIEKTEVKSCKVNAANLPGPKDLGLVLRDQSHCKAKKFP
                     NSPVKAEKATISQAKSEKATSLQAKAEKSPKSPNSVKAEKASSYQMKSEKVPSSPAEA
                     EKGPSLLLKDMRQKTELQQIGKKIPSSFTSVDKVNIEAVGGEKCALQNSPRSQKQQTC
                     TDNTGDSDDSASGIEDVSDDLSKMKNDESNKENSSEMDYLENATVIDESALTPEQRLG
                     LKQAEERLERDHIFRLEKRSPEYTNCRYLCKLCLIHIENIQGAHKHIKEKRHKKNILE
                     KQEESELRSLPPPSPAHLAALSVAVIELAKEHGITDDDLRVRQEIVEEMSKVITTFLP
                     ECSLRLYGSSLTRFALKSSDVNIDIKFPPKMNHPDLLIKVLGILKKNVLYVDVESDFH
                     AKVPVVVCRDRKSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCY
                     IDSQTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKGIVEEK
                     FVKWECNSSSATEKNSIAEENKAKADQPKDDTKKTETDNQSNAMKEKHGKSPLALETP
                     NRVSLGQLWLELLKFYTLDFALEEYVICVRIQDILTRENKNWPKRRIAIEDPFSVKRN
                     VARSLNSQLVYEYVVERFRAAYRYFACPQTKGGNKSTVDFKKREKGKISNKKPVKSNN
                     MATNGCILLGETTEKINAEREQPVQCDEMDCTSQRCIIDNNNLLVNELDFADHGQDSS
                     SLSTSKSSEIEPKLDKKQDDLAPSETCLKKELSQCNCIDLSKSPDPDKSTGTDCRSNL
                     ETESSHQSVCTDTSATSCNCKATEDASDLNDDDNLPTQELYYVFDKFILTSGKPPTIV
                     CSICKKDGHSKNDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDELSPPCSEQHNR
                     EQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLDICMTLEGHENAEKLNCKEI
                     IENLAKILKRHPGLRNILPITTAKVPIVKFEHRRSGLEGDISLYNTLAQHNTRMLATY
                     AAIDPRVQYLGYTMKVFAKRCDIGDASRGSLSSYAYILMVLYFLQQRKPPVIPVLQEI
                     FDGKQIPQRMVDGWNAFFFDKTEELKKRLPSLGKNTESLGELWLGLLRFYTEEFDFKE
                     YVISIRQKKLLTTFEKQWTSKCIAIEDPFDLNHNLGAGVSRKMTNFIMKAFINGRKLF
                     GTPFYPLIGREAEYFFDSRVLTDGELAPNDRCCRVCGKIGHYMKDCPKRKSSLLFRLK
                     KKDSEEEKEGNEEEKDSRDVLDPRDLHDTRDFRDPRDLRCFICGDAGHVRRECPEVKL
                     ARQRNSSVAAAQLVRNLVNAQQVAGSAQQQGDQSIRTRQSSECSESPSYSPQPQPFPQ
                     NSSQSAAITQPSSQPGSQPKLGPPQQGAQPPHQVQMPLYNFPQSPPAQYSPMHNMGLL
                     PMHPLQIPAPSWPIHGPVIHSAPGSAPSNIGLNDPSIIFAQPAARPVAIPNTSHDGHW
                     PRTVAPNSLVNSGAVGNSEPGFRGLTPPIPWEHAPRPHFPLVPASWPYGLHQNFMHQG
                     NARFQPNKPFYTQDRCATRRCRERCPHPPRGNVSE"
     exon            974..1137
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1254
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1432
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1521
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1573
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1643
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1644..1770
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1890
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..2103
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2278
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2982
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            2983..3133
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3134..3220
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3221..3324
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3325..3382
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3383..3486
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3487..3576
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3577..3690
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3691..3768
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3769..3946
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3947..3994
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            3995..4077
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            4078..4193
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            4194..4417
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            4418..4518
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            4519..4966
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            4967..5125
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     exon            5126..5853
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5710..5715
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /note="hexamer: AATAAA"
     polyA_site      5728
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /note="major polyA site"
     regulatory      5828..5833
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /note="hexamer: AATAAA"
     polyA_site      5853
                     /gene="TUT4"
                     /gene_synonym="PAPD3; TENT3A; ZCCHC11"
                     /note="major polyA site"
ORIGIN      
        1 agttcgcggc gcggcggaca tggcggtgga gtcctgagct agtcgtgtcc cggcctccag
       61 cggcggcagc ggcgacggcg gcggcagcag gaggcggagg gagagcagga gccactgaga
      121 tgaggaggcg tcgcgcgcgc ctgtagctcg cgggcctcgc agtttgcttg cctaaactgg
      181 acttgaagca atttaaacta ctggagatct gccattttat aatatcacaa ttggaaagaa
      241 acagattttc aaataatgga agagtctaaa accttaaaaa gtgaaaatca tgaaccaaag
      301 aagaatgtta tttgtgaaga aagcaaagca gttcaagtta taggtaatca aacattgaaa
      361 gctagaaatg ataaatccgt aaaagaaatt gagaacagct ctccaaatag gaatagtagt
      421 aaaaaaaata agcaaaatga tatttgtata gaaaaaacag aagttaaatc atgtaaagta
      481 aatgctgcca accttccagg tcctaaagat ttggggttag tccttcgaga tcaaagtcat
      541 tgcaaagcaa aaaaatttcc taattcaccg gtgaaagccg aaaaggcaac catttcacag
      601 gcaaaatcag aaaaggcaac cagtttacag gcaaaagcag aaaaatcacc aaagtcacct
      661 aattcagtga aagcagaaaa agcatccagt tatcagatga agtcagaaaa agtaccaagt
      721 tcaccagcag aagcagaaaa gggacccagt ttactgttga aagacatgag acagaagaca
      781 gaattacaac agattggaaa aaaaattcca agctccttta cttctgtgga caaagtgaat
      841 attgaagctg tagggggaga aaaatgtgct ctgcaaaact caccacgatc tcagaagcaa
      901 cagacatgta cagataatac tggtgattct gatgatagtg cttcaggaat tgaagacgta
      961 tcggacgatt taagtaaaat gaagaatgat gaatctaata aagaaaattc ttcagagatg
     1021 gactacttag aaaatgccac tgtgatagat gaatctgcat tgacacctga gcagaggctg
     1081 gggttgaaac aagcagaaga acgcttagaa agagatcaca tctttcgact tgaaaaacga
     1141 tcaccagaat ataccaattg tcggtatcta tgcaaacttt gcttaattca cattgaaaat
     1201 atccaggggg ctcataaaca tataaaggag aaacgacata agaaaaatat tttggaaaaa
     1261 caagaagaaa gtgagcttcg ttctctgcca cctccttccc ctgcccactt ggctgcttta
     1321 agtgttgcag tcattgaatt agcaaaagaa catggaataa cagatgatga cctcagagtc
     1381 cgtcaggaaa ttgtggagga aatgtcaaag gttataacga catttttacc agaatgttca
     1441 cttaggttgt atggctcatc tctgactaga tttgctctga aaagtagtga tgttaatata
     1501 gatataaaat ttcctcccaa gatgaatcat ccagatcttc tgataaaagt acttgggatt
     1561 ttaaagaaaa atgtattata tgtagatgtg gaatctgatt ttcacgctaa agttcctgtt
     1621 gtggtgtgca gagatcgaaa aagtggttta ctttgtagag tgagtgcagg aaacgatatg
     1681 gcatgtctca ctactgattt acttactgcc cttggcaaaa tagaacctgt ctttattccc
     1741 ttggtgttag cctttcgcta ctgggctaag ttgtgctata ttgactccca aactgatggt
     1801 ggaatccctt cttactgttt tgctttaatg gtgatgtttt ttctacaaca gagaaaaccc
     1861 cctcttcttc cttgcttact tggaagttgg attgaaggct ttgacccaaa aagaatggat
     1921 gactttcagc tgaagggcat agtagaagag aagtttgtga agtgggaatg taattcaagt
     1981 agtgcaactg agaaaaactc aattgctgag gaaaacaaag ctaaggcaga ccaaccaaaa
     2041 gatgatacca agaagacaga aacagacaac caaagtaatg ccatgaagga aaaacatggc
     2101 aaatctcctt tagcattgga aacaccaaat cgggtatcct tgggacagtt atggttagag
     2161 ctgcttaaat tctacacact ggattttgct ttggaggaat atgtcatatg tgtacggata
     2221 caagatattt taacaagaga aaataaaaac tggcctaaaa ggcgaatagc cattgaagat
     2281 ccattttcag tcaagaggaa tgttgcacgg agcttaaaca gccagctggt ttacgaatat
     2341 gttgtggaga gatttagggc agcttatcgg tattttgcct gtcctcagac gaagggtgga
     2401 aataagtcta cagtggattt caagaaaaga gagaagggga aaataagcaa taagaaacca
     2461 gtcaagtcga acaatatggc aaccaatggt tgtattctgc ttggggaaac cacagaaaaa
     2521 ataaatgcag aaagagagca acctgttcaa tgtgatgaaa tggactgtac atcacagaga
     2581 tgtattattg acaacaacaa tttgttggta aatgaactag attttgctga ccacggacag
     2641 gactcttcat ctctttctac cagcaaaagc agtgaaatag agccaaaatt agataagaaa
     2701 caagatgatt tagcgccttc agaaacttgt ttaaaaaaag agctcagcca atgtaattgc
     2761 attgatttgt ctaagtcgcc tgacccagat aaatctactg gaacagactg caggtcaaat
     2821 ttagaaacag agagttcaca tcagagtgtg tgcaccgaca catctgctac ctcttgcaac
     2881 tgcaaagcta cagaagatgc ttctgacctt aatgatgatg ataacctccc cacccaggaa
     2941 ttatattatg tgtttgataa gtttatttta acctctggca agccaccaac gatagtatgc
     3001 agcatctgca aaaaggatgg ccattcaaag aatgattgcc cagaggattt taggaaaatt
     3061 gatttaaaac ctctaccacc aatgacaaac cgatttcggg aaatacttga tttagtatgt
     3121 aaaagatgtt ttgatgagtt atcaccacct tgttctgaac aacacaacag ggagcaaatt
     3181 ttaattggct tggaaaagtt tattcaaaaa gaatatgatg aaaaggcaag gttgtgctta
     3241 tttggctctt ctaagaatgg atttggattt cgtgatagtg atctggatat ttgtatgacc
     3301 ctggaaggcc atgaaaatgc agagaaatta aattgtaagg aaataattga aaatttggca
     3361 aaaattctta agagacatcc aggtttaaga aacattttgc ctataactac tgccaaagtg
     3421 cctatagtaa aatttgaaca caggcgaagt gggttagaag gcgatatcag tttatataat
     3481 acgttggctc aacataacac aagaatgcta gctacttatg cagctattga tcctagagtg
     3541 cagtatttgg gatatactat gaaagtgttt gctaagcgat gtgacattgg ggatgcttcc
     3601 aggggaagtt tatcttcata tgcatatatc cttatggtgc tgtactttct gcagcagaga
     3661 aagccacctg ttatcccagt tctacaagag atctttgatg gaaaacagat tccacagaga
     3721 atggttgatg gatggaatgc tttcttcttt gataaaacag aagaactgaa aaagcgttta
     3781 ccttcacttg gaaagaacac agaatcatta ggggagcttt ggctgggact gcttcgtttc
     3841 tatactgaag agtttgattt caaggaatat gtaattagca ttcggcagaa aaagctgttg
     3901 acaacttttg agaagcagtg gacttccaag tgcattgcaa ttgaagaccc ttttgacttg
     3961 aatcataacc ttggtgctgg agtttccaga aaaatgacca atttcatcat gaaagcattt
     4021 atcaatggaa ggaaactttt tggtacccct ttttatccac tcattggcag agaagctgag
     4081 tacttctttg attccagagt attaacagat ggagaactgg ctcccaatga tagatgttgc
     4141 cgtgtgtgtg gaaaaatagg ccactacatg aaagactgcc ctaaaaggaa aagcagttta
     4201 ctgtttagac taaagaagaa agacagtgaa gaagagaagg aagggaatga agaagagaaa
     4261 gattcccgag atgttcttga cccccgagac ctccacgata ctcgagactt tagagacccg
     4321 agagacctca gatgttttat atgtggagat gctggacatg tacgaaggga gtgcccagag
     4381 gtcaagctgg cccgtcagag gaatagcagt gtggcagcag cccagctggt ccgcaacctt
     4441 gtaaatgctc aacaggtggc tggttcagct cagcaacagg gtgatcagtc cataaggact
     4501 agacagtcat cagaatgttc tgaatcacca tcttattctc ctcagcctca gccatttcca
     4561 cagaactctt cccagtcagc tgctattact cagccttcat ctcagccagg atcccaacct
     4621 aagcttggcc cacctcagca gggagcccaa cctccccatc aggtccagat gccactgtat
     4681 aactttcccc agtcaccacc agctcagtat tctcccatgc acaatatggg attgttgcca
     4741 atgcaccctc tccagatccc tgccccgtcc tggcccatcc atggcccagt gatccactct
     4801 gcaccaggca gtgcccccag caatattggc ctaaatgatc ccagcatcat ctttgcacag
     4861 cctgctgcca gacctgtggc aatccctaac acgtctcacg atggacactg gccccgtact
     4921 gtggctccaa attccctggt aaacagtggt gcagtgggga attcagagcc aggctttcga
     4981 ggactgactc ctccaattcc ttgggaacat gcaccgcgtc cccatttccc ccttgtccca
     5041 gcttcgtggc cttatggttt gcatcaaaac ttcatgcatc agggaaatgc ccgattccag
     5101 cccaacaaac ctttctatac tcaagacaga tgtgccaccc gtcggtgtag agagcgttgt
     5161 ccccacccac caagaggaaa cgtgtcggag taatgcgagt ccattttctt tcagctggtc
     5221 taccgatgca cagcaaccag ccaatcctgc tgtctcaagg gtatccgtac ctcaatgtca
     5281 gttacattca gcagaaaaag tgacatttaa tggaaagcaa aacaaatcag gtcctgattt
     5341 aaaattttaa cacgtttaga tagcttattt aaattctaag actgttttta cagactgtat
     5401 tatttgtata taaatatgaa ctatagtttt tactagtatc accaaattga gtgatacaaa
     5461 tttcatctat tttattaccc tatttttaag ggaattttat gttttgttta accttgatga
     5521 attgtataaa gagagaattt aaaaaattac attctttatt ttctattagc tgtattcata
     5581 ctttggttgc ttcagacttt atatattgtt cttaaaagtt aggaaagaat ttcatgtgtt
     5641 ttaaattgtc acttctattc tttacagaac cttgtagtgc caaaaacttt ttaaaaggta
     5701 aaaaaataaa ataaaattac aacatgaaga ttcagatttt ttttcttttc catacttgta
     5761 tattgaacat tcaaacttga atggaaagtt gattttgtct gtatttgaag tacttctatt
     5821 ttaagttaat aaatcttttt gacaagagtt aaa
//
