LOCUS       NM_006765               3697 bp    mRNA    linear   PRI 15-MAR-2025
DEFINITION  Homo sapiens tumor suppressor candidate 3 (TUSC3), transcript
            variant 1, mRNA.
ACCESSION   NM_006765
VERSION     NM_006765.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Deng,R., Lu,X., Hong,C., Cai,R., Wang,P., Xiong,L., Wang,X.,
            Chen,Q. and Lin,J.
  TITLE     Downregulation of TUSC3 promotes EMT and hepatocellular carcinoma
            progression through LIPC/AKT axis
  JOURNAL   J Transl Med 20 (1), 485 (2022)
   PUBMED   36274132
  REMARK    GeneRIF: Downregulation of TUSC3 promotes EMT and hepatocellular
            carcinoma progression through LIPC/AKT axis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Sun,F., Jie,Q., Li,Q., Wei,Y., Li,H., Yue,X. and Ma,Y.
  TITLE     TUSC3 inhibits cell proliferation and invasion in cervical squamous
            cell carcinoma via suppression of the AKT signalling pathway
  JOURNAL   J Cell Mol Med 26 (5), 1629-1642 (2022)
   PUBMED   35137520
  REMARK    GeneRIF: TUSC3 inhibits cell proliferation and invasion in cervical
            squamous cell carcinoma via suppression of the AKT signalling
            pathway.
REFERENCE   3  (bases 1 to 3697)
  AUTHORS   Ren,Y., Deng,R., Cai,R., Lu,X., Luo,Y., Wang,Z., Zhu,Y., Yin,M.,
            Ding,Y. and Lin,J.
  TITLE     TUSC3 induces drug resistance and cellular stemness via Hedgehog
            signaling pathway in colorectal cancer
  JOURNAL   Carcinogenesis 41 (12), 1755-1766 (2020)
   PUBMED   32338281
  REMARK    GeneRIF: TUSC3 induces drug resistance and cellular stemness via
            Hedgehog signaling pathway in colorectal cancer.
REFERENCE   4  (bases 1 to 3697)
  AUTHORS   Ouidir,M., Mendola,P., Buck Louis,G.M., Kannan,K., Zhang,C. and
            Tekola-Ayele,F.
  TITLE     Concentrations of persistent organic pollutants in maternal plasma
            and epigenome-wide placental DNA methylation
  JOURNAL   Clin Epigenetics 12 (1), 103 (2020)
   PUBMED   32653021
  REMARK    GeneRIF: Concentrations of persistent organic pollutants in
            maternal plasma and epigenome-wide placental DNA methylation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3697)
  AUTHORS   Wang,S. and Zhu,W.
  TITLE     Tumour suppressor candidate 3 inhibits biological function and
            increases endoplasmic reticulum stress of melanoma cells WM451 by
            regulating AKT/GSK3-beta/beta-catenin pathway
  JOURNAL   Cell Biochem Funct 38 (5), 604-612 (2020)
   PUBMED   32090352
  REMARK    GeneRIF: Tumour suppressor candidate 3 inhibits biological function
            and increases endoplasmic reticulum stress of melanoma cells WM451
            by regulating AKT/GSK3-beta/beta-catenin pathway.
REFERENCE   6  (bases 1 to 3697)
  AUTHORS   Kelleher,D.J., Karaoglu,D., Mandon,E.C. and Gilmore,R.
  TITLE     Oligosaccharyltransferase isoforms that contain different catalytic
            STT3 subunits have distinct enzymatic properties
  JOURNAL   Mol Cell 12 (1), 101-111 (2003)
   PUBMED   12887896
REFERENCE   7  (bases 1 to 3697)
  AUTHORS   Ishii,H., Baffa,R., Numata,S.I., Murakumo,Y., Rattan,S., Inoue,H.,
            Mori,M., Fidanza,V., Alder,H. and Croce,C.M.
  TITLE     The FEZ1 gene at chromosome 8p22 encodes a leucine-zipper protein,
            and its expression is altered in multiple human tumors
  JOURNAL   Proc Natl Acad Sci U S A 96 (7), 3928-3933 (1999)
   PUBMED   10097140
REFERENCE   8  (bases 1 to 3697)
  AUTHORS   Pak,B.J., Park,H., Chang,E.R., Pang,S.C. and Graham,C.H.
  TITLE     Differential display analysis of oxygen-mediated changes in gene
            expression in first trimester human trophoblast cells
  JOURNAL   Placenta 19 (7), 483-488 (1998)
   PUBMED   9778121
REFERENCE   9  (bases 1 to 3697)
  AUTHORS   MacGrogan,D., Levy,A., Bova,G.S., Isaacs,W.B. and Bookstein,R.
  TITLE     Structure and methylation-associated silencing of a gene within a
            homozygously deleted region of human chromosome band 8p22
  JOURNAL   Genomics 35 (1), 55-65 (1996)
   PUBMED   8661104
REFERENCE   10 (bases 1 to 3697)
  AUTHORS   Sparks,S.E. and Krasnewich,D.M.
  TITLE     Congenital Disorders of N-Linked Glycosylation and Multiple Pathway
            Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301507
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA796279.1, U42349.1,
            AC019292.10 and AI366810.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_006765.3.
            
            Summary: This gene encodes a protein that has been associated with
            several biological functions including cellular magnesium uptake,
            protein glycosylation and embryonic development. This protein
            localizes to the endoplasmic reticulum and acts as a component of
            the oligosaccharyl transferase complex which is responsible for
            N-linked protein glycosylation. This gene is a candidate tumor
            suppressor gene. Homozygous mutations in this gene are associated
            with autosomal recessive nonsyndromic mental retardation-7 and in
            the proliferation and invasiveness of several cancers including
            metastatic pancreatic cancer, ovarian cancer and glioblastoma
            multiform. [provided by RefSeq, Oct 2017].
            
            Transcript Variant: This variant (1) represents the longer
            transcript, and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.190050.1,
                                           SRR1660805.203313.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2151741, SAMN03568911
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000503731.6/ ENSP00000424544.1
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                DA796279.1         177-196
            21-1262             U42349.1           10-1251
            1263-3272           AC019292.10        44382-46391
            3273-3697           AI366810.1         16-440              c
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p22"
     gene            1..3697
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="tumor suppressor candidate 3"
                     /db_xref="GeneID:7991"
                     /db_xref="HGNC:HGNC:30242"
                     /db_xref="MIM:601385"
     exon            1..306
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             169..1215
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; oligosaccharyltransferase 3 homolog A;
                     magnesium uptake/transporter TUSC3; putative prostate
                     cancer tumor suppressor; oligosaccharyl transferase
                     subunit TUSC3; dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit TUSC3"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit TUSC3 isoform 1 precursor"
                     /protein_id="NP_006756.2"
                     /db_xref="CCDS:CCDS5994.1"
                     /db_xref="GeneID:7991"
                     /db_xref="HGNC:HGNC:30242"
                     /db_xref="MIM:601385"
                     /translation="MGARGAPSRRRQAGRRLRYLPTGSFPFLLLLLLLCIQLGGGQKK
                     KENLLAEKVEQLMEWSSRRSIFRMNGDKFRKFIKAPPRNYSMIVMFTALQPQRQCSVC
                     RQANEEYQILANSWRYSSAFCNKLFFSMVDYDEGTDVFQQLNMNSAPTFMHFPPKGRP
                     KRADTFDLQRIGFAAEQLAKWIADRTDVHIRVFRPPNYSGTIALALLVSLVGGLLYLR
                     RNNLEFIYNKTGWAMVSLCIVFAMTSGQMWNHIRGPPYAHKNPHNGQVSYIHGSSQAQ
                     FVAESHIILVLNAAITMGMVLLNEAATSKGDVGKRRIICLVGLGLVVFFFSFLLSIFR
                     SKYHGYPYSDLDFE"
     exon            307..476
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            477..594
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            595..735
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            736..876
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            877..966
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1030
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1105
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1196
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1261
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..3697
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1522..1527
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: AATAAA"
     polyA_site      1545
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="major polyA site"
     regulatory      1681..1686
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: ATTAAA"
     polyA_site      1724
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     regulatory      2298..2303
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: AATAAA"
     polyA_site      2332
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     regulatory      2946..2951
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: ATTAAA"
     polyA_site      2967
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     regulatory      3024..3029
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: ATTAAA"
     polyA_site      3044
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     regulatory      3528..3533
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: CATAAA"
     polyA_site      3551
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     regulatory      3591..3596
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: TATAAA"
     polyA_site      3602
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     polyA_site      3611
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     polyA_site      3642
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
ORIGIN      
        1 agtctcctcc tctgcgtcct cggccgcggc ccgggtccct cgcaaagccg ctgccatccc
       61 ggagggccca gccagcgggc tcccggaggc tggccgggca ggcgtggtgc gcggtaggag
      121 ctgggcgcgc acggctaccg cgcgtggagg agacactgcc ctgccgcgat gggggcccgg
      181 ggcgctcctt cacgccgtag gcaagcgggg cggcggctgc ggtacctgcc caccgggagc
      241 tttcccttcc ttctcctgct gctgctgctc tgcatccagc tcgggggagg acagaagaaa
      301 aaggagaatc ttttagctga aaaagtagag cagctgatgg aatggagttc cagacgctca
      361 atcttccgaa tgaatggtga taaattccga aaatttataa aggcaccacc tcgaaactat
      421 tccatgattg ttatgttcac tgctcttcag cctcagcggc agtgttctgt gtgcaggcaa
      481 gctaatgaag aatatcaaat actggcgaac tcctggcgct attcatctgc tttttgtaac
      541 aagctcttct tcagtatggt ggactatgat gaggggacag acgtttttca gcagctcaac
      601 atgaactctg ctcctacatt catgcatttt cctccaaaag gcagacctaa gagagctgat
      661 acttttgacc tccaaagaat tggatttgca gctgagcaac tagcaaagtg gattgctgac
      721 agaacggatg ttcatattcg ggttttcaga ccacccaact actctggtac cattgctttg
      781 gccctgttag tgtcgcttgt tggaggtttg ctttatttga gaaggaacaa cttggagttc
      841 atctataaca agactggttg ggccatggtg tctctgtgta tagtctttgc tatgacttct
      901 ggccagatgt ggaaccatat ccgtggacct ccatatgctc ataagaaccc acacaatgga
      961 caagtgagct acattcatgg gagcagccag gctcagtttg tggcagaatc acacattatt
     1021 ctggtactga atgccgctat caccatgggg atggttcttc taaatgaagc agcaacttcg
     1081 aaaggcgatg ttggaaaaag acggataatt tgcctagtgg gattgggcct ggtggtcttc
     1141 ttcttcagtt ttctactttc aatatttcgt tccaagtacc acggctatcc ttatagtgat
     1201 ctggactttg agtgagaaga tgtgatttgg accatggcac ttaaaaactc tataacctca
     1261 gctttttaat taaatgaagc caagtgggat ttgcataaag tgaatgttta ccatgaagat
     1321 aaactgttcc tgactttata ctattttgaa ttcattcatt tcattgtgat cagctagctt
     1381 attcttgtgt acttttttta aactgtgggt tttcctagta aatttaattt acagaaatca
     1441 atggtagcat ttagtaatct acaaaggaaa tatcaaagtg tttttcaagc ctgttatatt
     1501 cagtgtgtgc cacaggattg aaataaatga caatgtaatt atgaattcat gttttagagc
     1561 tgtttactca ttagtaaagg accgcaatgt tagtaaagaa aacctatgaa atgtatgtta
     1621 aagattctta gtattgtaca gggataaagc aaatgcatga aaatatgtca tgtactgaaa
     1681 attaaactta cttcagtttc agttataaag gacgtgcact gttacgaata gtgttttgtt
     1741 attaattata actagataaa ggtatatagg acagggaact ggatgaatgg tacctacaat
     1801 ttggatgcct aaccaaggac tagagctcct tcttgagatc taaatctaaa gtaaatgtgc
     1861 attaaagcag tgtgcttcaa aggcatcaga cgatgaaagc aacataccac aactaggagt
     1921 tatttctcaa acttaaatgt cctctgggaa tccagactta aaaataagag caaacttaac
     1981 acactatcca ttttcgagca aacctaaccc actatatcca ttttgctcat gtgttttatg
     2041 caaccagctt tccatcaaat cctcaatctt tgaatccagg taaaaggtta attatcctag
     2101 gattagtgaa tgattcaatg aagctttctt gaaaacaaac ataggagtgt aatgtactat
     2161 tatgtttgta tcctgtttta gtttataaag cactttcaca tacattatgg tatttcttcc
     2221 tcacaataac cttgtaaatt agtcaggaaa tgtaaatttt atctgactca tagaactaat
     2281 ggaagctgaa agccaaaaat aaagtttata accaggcctt caaactctca aacatggatt
     2341 tttatattct gtggagttat ccaaattaat tagggtttag gaaagcagtt cttcttgagt
     2401 cagttcaata agtgtttgct ttttgttttt aaggtttgtt atctaatttt cattaagatt
     2461 tgagtaacag accatggaga attgttttca ttgggagttc cagctatata gcctcaaaca
     2521 agaaacgggt gtacaaccat tgagtttact tacttaaatc tgtgaatttc aatgaagaat
     2581 gggaatataa aggcctactg gggcatgttt atcaagttat atcccagggc aatgtgtatg
     2641 ctagcaggat gaatgctatc ttttcaaaac ataatattca gtgaaaatta cgtaataatt
     2701 gtaagtttat aatcatactc ccaaatctgt tactaaaaat aacataaatt ctcctagttc
     2761 atgtacttag atgaataaat ttagttttgg aatgacgttt atggaaaagc aatagatgaa
     2821 cttaatcatt tgatcactgc ctatcactag gcagaacttg acctgtaatt cttaatccat
     2881 tgtagatttt ttacatttta ctcaaaactt catacataga gactctcagg tcaaatttta
     2941 caagtattaa acatttgtta attataatca cttttgtttg tatccttaaa tctcagaatt
     3001 atcttgcagt taatatgcag ctgattaaat gatattacaa attatctcta atctcactgt
     3061 aaatctttta atcatatcaa agtcaggtca tcctcagaca ctataactaa gatagataag
     3121 gagtacttta ctataccata ttagtaaagg ttttctagct cttattttct aatttcctct
     3181 gagcatttaa aattacatcc agtgataaaa acaatatttt tttttcagaa tttcatgctt
     3241 taaaaagctg tggcttctct atagacaact gttacattag ggaagtgatt ctagagcaaa
     3301 atatactgcc tcaacataag tcgttactga ctggaaaatg ctataggcag aattgcaaag
     3361 agtggctcaa acatatagtt tgcttgcaga atgtaacgtg caaaatcaca tacatgcgat
     3421 gtatttacct ttgctagaca atgagaattg aataattgat ttgtacctgg tatgttatat
     3481 cctccagtgt cactttttaa ccagattcac tgtgcgttcc aaactgtcat aaaaattgat
     3541 tatatgttta acaattgttt ttctactaca gtggagagaa aaatcccaat tataaaattc
     3601 cacctaaagg agtttcattt tgtaataaca tctgattaat aacttgttgc attttgctta
     3661 acatactgtt cctggcatta gatattcaca tgagtaa
//
