LOCUS       NM_001413684            4505 bp    mRNA    linear   PRI 15-MAR-2025
DEFINITION  Homo sapiens tumor suppressor candidate 3 (TUSC3), transcript
            variant 20, mRNA.
ACCESSION   NM_001413684
VERSION     NM_001413684.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Deng,R., Lu,X., Hong,C., Cai,R., Wang,P., Xiong,L., Wang,X.,
            Chen,Q. and Lin,J.
  TITLE     Downregulation of TUSC3 promotes EMT and hepatocellular carcinoma
            progression through LIPC/AKT axis
  JOURNAL   J Transl Med 20 (1), 485 (2022)
   PUBMED   36274132
  REMARK    GeneRIF: Downregulation of TUSC3 promotes EMT and hepatocellular
            carcinoma progression through LIPC/AKT axis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Sun,F., Jie,Q., Li,Q., Wei,Y., Li,H., Yue,X. and Ma,Y.
  TITLE     TUSC3 inhibits cell proliferation and invasion in cervical squamous
            cell carcinoma via suppression of the AKT signalling pathway
  JOURNAL   J Cell Mol Med 26 (5), 1629-1642 (2022)
   PUBMED   35137520
  REMARK    GeneRIF: TUSC3 inhibits cell proliferation and invasion in cervical
            squamous cell carcinoma via suppression of the AKT signalling
            pathway.
REFERENCE   3  (bases 1 to 4505)
  AUTHORS   Ren,Y., Deng,R., Cai,R., Lu,X., Luo,Y., Wang,Z., Zhu,Y., Yin,M.,
            Ding,Y. and Lin,J.
  TITLE     TUSC3 induces drug resistance and cellular stemness via Hedgehog
            signaling pathway in colorectal cancer
  JOURNAL   Carcinogenesis 41 (12), 1755-1766 (2020)
   PUBMED   32338281
  REMARK    GeneRIF: TUSC3 induces drug resistance and cellular stemness via
            Hedgehog signaling pathway in colorectal cancer.
REFERENCE   4  (bases 1 to 4505)
  AUTHORS   Ouidir,M., Mendola,P., Buck Louis,G.M., Kannan,K., Zhang,C. and
            Tekola-Ayele,F.
  TITLE     Concentrations of persistent organic pollutants in maternal plasma
            and epigenome-wide placental DNA methylation
  JOURNAL   Clin Epigenetics 12 (1), 103 (2020)
   PUBMED   32653021
  REMARK    GeneRIF: Concentrations of persistent organic pollutants in
            maternal plasma and epigenome-wide placental DNA methylation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4505)
  AUTHORS   Wang,S. and Zhu,W.
  TITLE     Tumour suppressor candidate 3 inhibits biological function and
            increases endoplasmic reticulum stress of melanoma cells WM451 by
            regulating AKT/GSK3-beta/beta-catenin pathway
  JOURNAL   Cell Biochem Funct 38 (5), 604-612 (2020)
   PUBMED   32090352
  REMARK    GeneRIF: Tumour suppressor candidate 3 inhibits biological function
            and increases endoplasmic reticulum stress of melanoma cells WM451
            by regulating AKT/GSK3-beta/beta-catenin pathway.
REFERENCE   6  (bases 1 to 4505)
  AUTHORS   Kelleher,D.J., Karaoglu,D., Mandon,E.C. and Gilmore,R.
  TITLE     Oligosaccharyltransferase isoforms that contain different catalytic
            STT3 subunits have distinct enzymatic properties
  JOURNAL   Mol Cell 12 (1), 101-111 (2003)
   PUBMED   12887896
REFERENCE   7  (bases 1 to 4505)
  AUTHORS   Ishii,H., Baffa,R., Numata,S.I., Murakumo,Y., Rattan,S., Inoue,H.,
            Mori,M., Fidanza,V., Alder,H. and Croce,C.M.
  TITLE     The FEZ1 gene at chromosome 8p22 encodes a leucine-zipper protein,
            and its expression is altered in multiple human tumors
  JOURNAL   Proc Natl Acad Sci U S A 96 (7), 3928-3933 (1999)
   PUBMED   10097140
REFERENCE   8  (bases 1 to 4505)
  AUTHORS   Pak,B.J., Park,H., Chang,E.R., Pang,S.C. and Graham,C.H.
  TITLE     Differential display analysis of oxygen-mediated changes in gene
            expression in first trimester human trophoblast cells
  JOURNAL   Placenta 19 (7), 483-488 (1998)
   PUBMED   9778121
REFERENCE   9  (bases 1 to 4505)
  AUTHORS   MacGrogan,D., Levy,A., Bova,G.S., Isaacs,W.B. and Bookstein,R.
  TITLE     Structure and methylation-associated silencing of a gene within a
            homozygously deleted region of human chromosome band 8p22
  JOURNAL   Genomics 35 (1), 55-65 (1996)
   PUBMED   8661104
REFERENCE   10 (bases 1 to 4505)
  AUTHORS   Sparks,S.E. and Krasnewich,D.M.
  TITLE     Congenital Disorders of N-Linked Glycosylation and Multiple Pathway
            Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301507
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC091559.7, AC010656.7 and
            AC019292.10.
            
            Summary: This gene encodes a protein that has been associated with
            several biological functions including cellular magnesium uptake,
            protein glycosylation and embryonic development. This protein
            localizes to the endoplasmic reticulum and acts as a component of
            the oligosaccharyl transferase complex which is responsible for
            N-linked protein glycosylation. This gene is a candidate tumor
            suppressor gene. Homozygous mutations in this gene are associated
            with autosomal recessive nonsyndromic mental retardation-7 and in
            the proliferation and invasiveness of several cancers including
            metastatic pancreatic cancer, ovarian cancer and glioblastoma
            multiform. [provided by RefSeq, Oct 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.4495075.1,
                                           SRR1803614.90758.1 [ECO:0000332]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-306               AC091559.7         162788-163093
            307-476             AC010656.7         58176-58345         c
            477-594             AC010656.7         30611-30728         c
            595-735             AC010656.7         21778-21918         c
            736-876             AC010656.7         19129-19269         c
            877-966             AC010656.7         7589-7678           c
            967-1030            AC019292.10        10844-10907
            1031-1105           AC019292.10        23716-23790
            1106-1196           AC019292.10        28553-28643
            1197-4505           AC019292.10        86541-89849
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p22"
     gene            1..4505
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="tumor suppressor candidate 3"
                     /db_xref="GeneID:7991"
                     /db_xref="HGNC:HGNC:30242"
                     /db_xref="MIM:601385"
     exon            1..306
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             169..1353
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="isoform 17 precursor is encoded by transcript
                     variant 20; oligosaccharyltransferase 3 homolog A;
                     magnesium uptake/transporter TUSC3; putative prostate
                     cancer tumor suppressor; oligosaccharyl transferase
                     subunit TUSC3; dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit TUSC3"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit TUSC3 isoform 17 precursor"
                     /protein_id="NP_001400613.1"
                     /db_xref="GeneID:7991"
                     /db_xref="HGNC:HGNC:30242"
                     /db_xref="MIM:601385"
                     /translation="MGARGAPSRRRQAGRRLRYLPTGSFPFLLLLLLLCIQLGGGQKK
                     KENLLAEKVEQLMEWSSRRSIFRMNGDKFRKFIKAPPRNYSMIVMFTALQPQRQCSVC
                     RQANEEYQILANSWRYSSAFCNKLFFSMVDYDEGTDVFQQLNMNSAPTFMHFPPKGRP
                     KRADTFDLQRIGFAAEQLAKWIADRTDVHIRVFRPPNYSGTIALALLVSLVGGLLYLR
                     RNNLEFIYNKTGWAMVSLCIVFAMTSGQMWNHIRGPPYAHKNPHNGQVSYIHGSSQAQ
                     FVAESHIILVLNAAITMGMVLLNEAATSKGDVGKRRIICLVGLGLVVFFFSFLLSIFR
                     SKYHGYPYRYLPNSASFFAASGDTLGAFGNQRKKSHTCCLDFPYANRCRLFHCKLSQG
                     HQ"
     exon            307..476
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            477..594
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            595..735
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            736..876
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            877..966
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1030
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1105
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1196
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..4505
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3317..3322
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: AATATA"
     polyA_site      3339
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     regulatory      4485..4490
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: AATAAA"
     polyA_site      4505
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="major polyA site"
ORIGIN      
        1 agtctcctcc tctgcgtcct cggccgcggc ccgggtccct cgcaaagccg ctgccatccc
       61 ggagggccca gccagcgggc tcccggaggc tggccgggca ggcgtggtgc gcggtaggag
      121 ctgggcgcgc acggctaccg cgcgtggagg agacactgcc ctgccgcgat gggggcccgg
      181 ggcgctcctt cacgccgtag gcaagcgggg cggcggctgc ggtacctgcc caccgggagc
      241 tttcccttcc ttctcctgct gctgctgctc tgcatccagc tcgggggagg acagaagaaa
      301 aaggagaatc ttttagctga aaaagtagag cagctgatgg aatggagttc cagacgctca
      361 atcttccgaa tgaatggtga taaattccga aaatttataa aggcaccacc tcgaaactat
      421 tccatgattg ttatgttcac tgctcttcag cctcagcggc agtgttctgt gtgcaggcaa
      481 gctaatgaag aatatcaaat actggcgaac tcctggcgct attcatctgc tttttgtaac
      541 aagctcttct tcagtatggt ggactatgat gaggggacag acgtttttca gcagctcaac
      601 atgaactctg ctcctacatt catgcatttt cctccaaaag gcagacctaa gagagctgat
      661 acttttgacc tccaaagaat tggatttgca gctgagcaac tagcaaagtg gattgctgac
      721 agaacggatg ttcatattcg ggttttcaga ccacccaact actctggtac cattgctttg
      781 gccctgttag tgtcgcttgt tggaggtttg ctttatttga gaaggaacaa cttggagttc
      841 atctataaca agactggttg ggccatggtg tctctgtgta tagtctttgc tatgacttct
      901 ggccagatgt ggaaccatat ccgtggacct ccatatgctc ataagaaccc acacaatgga
      961 caagtgagct acattcatgg gagcagccag gctcagtttg tggcagaatc acacattatt
     1021 ctggtactga atgccgctat caccatgggg atggttcttc taaatgaagc agcaacttcg
     1081 aaaggcgatg ttggaaaaag acggataatt tgcctagtgg gattgggcct ggtggtcttc
     1141 ttcttcagtt ttctactttc aatatttcgt tccaagtacc acggctatcc ttataggtac
     1201 ttgcccaact ctgcctcctt cttcgctgct tctggtgata ctttgggtgc atttggaaac
     1261 cagagaaaaa aatcacacac atgttgtctt gacttccctt atgcaaacag gtgtcgacta
     1321 tttcattgta aactttctca gggtcatcag tgacttcaag cctggatctt acaaaatcac
     1381 agagctcttc atttcccata acatcccaga tactatcaca tgcaaggata atgaagtgat
     1441 catcttcttc agatctttca gtgtcatgga cttcaggctc tggtgagaca agctgcccag
     1501 taggaccttt cccatggata catttgtaat caaaatcccc aagggctttg gatacagcca
     1561 gagaggcttt cacatgctaa agcattacag agccacctgc attctgaatt cattcttttt
     1621 ctagcggatt tgcaatttgt gaagaaatga actttcctgt tcctacaaag taaacgtcta
     1681 gagtcttcat agttaatgaa ataattatgt tggggagaaa gtaagacgct tacatctgtt
     1741 gacccacttc tatctgcacc atgtttcttc tctgacataa ctctcgtgtt catcaatctc
     1801 caggaaacct gttctgattc cattctttac attttccaaa gaaggtgctc ctgcagaccc
     1861 tgtaaatcct ggttatcggc gatgtgatct aacaaatgct cacagcagta tttggcaacc
     1921 tgagaaccag gatgcccgtt atacacagca aagaatgacc acctttcaag tccacttggc
     1981 aaagcaatca cagccgtatg tgcatcctcc atttcaatac gtcaaccttg catgctgcgt
     2041 agctcgtatc gcaactaatt accccacccc caggcattat gctcttccat ctttgcttgt
     2101 ctaaagatgg tcccattatg tcataatcct gtaggtctct attaaaagtc aaaaatcatc
     2161 aacaaaacca aaacaagtca acaaccacaa caacaacaac aaaacaaggc tgtggagaga
     2221 aggcaatgct tatacactgt tggtgggaat gtaaatggat tcagccactg tggaaagcaa
     2281 agaaattaaa gcttttaatg tctcaaagaa tgtaaaacag aactaccgtt cgacccagaa
     2341 atcccactac tgagtatata tccaaaggaa aataaatcgt tctcccaaaa agacatatac
     2401 ttttatgttc ataacagtgc tgtttactat agcacagtaa ttaacctggg tgcccatccg
     2461 tggtggattg gataaagaaa atgtggtaca tatgcatgtt ggactactaa acaccataca
     2521 aaagaacaaa agcatgtcct ttgcagcaat gtggatgcag ctggaggcca ttatcccaaa
     2581 caaattaaca caggaacaga aaaacaaata ctgcatgttc tcatttataa gtgggagcta
     2641 aacattgaga actcatgaac ataaatatgg caacaataga cactggggac tcacagaatg
     2701 cggatgaagg aaggaaagca agagttgcaa acctaacttt tgggtactac attctatatc
     2761 tgggggacag agcatttgca tccccaacct tagcatcgtg tgatataacc atgtcacata
     2821 cctgtatatg tacctctatt gaatctaaaa tgaaagttga aattattttt aaaaacttta
     2881 tgctagtata attttaaagc atcctacata tactagcttt tgaacatatt attacagtac
     2941 tcatatcatt ttattgtaga gattagggaa gctttgtttt ctgaccagta ttagaaaaga
     3001 tttgagtaac aattacctgc aatctttaag caaacttctt tcacctggta taatgtgttc
     3061 cttaagaaac ttaagaattt ttcaaagact ttttgtttca aatattggtt attattttaa
     3121 tactaaatgt tattttaata tattaaacat aaaaagaata taattgttta aaaaatgctc
     3181 tctccagagc actaggagat aaaatgatac atttacttat gtaacacttt cttaatattc
     3241 ctttgcattt tattactatc agtgagagtt tttgatattg gacaaagaaa gcttaagggg
     3301 ttagaaaagt tctctcaata tatatctctt gttggccaat tctgcttcca gccttttaat
     3361 gagactgtcc agaattattc tcttaccgaa catcttttcc tactctcaga cctgccatcg
     3421 ttacatcttc cacgaaggta agaacatgga agacagaagc agcccaagca taagtctttt
     3481 atagttccca ggagtaagag tgccagacat gtagcaggca atttgcccag tggcttctcc
     3541 atttttgaca atttctaagc tcagctgtca gtcagtgtta gctttggcct gctgccaccc
     3601 tagaaattct gcctcattct gagctcttgg tcacctggat tgctaattgt gcttccaggg
     3661 attctgaagc tcctcaacct acacaggttg tcttttttta aaaaaagata acatgtattt
     3721 atagagtaca aacaaattat acacacacac atataattaa gatatatata taattatagc
     3781 tattttgtat agtatgtaca gtagtacccc tgtaactaaa cccaagtttg gctcctcact
     3841 gcttgaaagg ccaaactcaa gagacaaggg ttggtgggag gaaaagcacg tttattcaga
     3901 aagccagcaa acccagatga tggcacacta tcatgctaaa gtaccatctt aagtcattcc
     3961 aaatttttgg ctctttttat gtcaacggca ggaggaagat ggagggctca gggcaagagg
     4021 taactgatga ctgcagatat ctgggcacca gtgagggtgt gaggatgttg ggaacttctt
     4081 ttccctggtc aggtcacagt atttccataa atctttaaca aaacatagtt acttgtcctt
     4141 taaactcaga gttctttctg aaaaactaca tgattgctgt ttttgtgtat gatctcactg
     4201 ctctataatt atccttgcct atctgtggga ataggtaaag gtcccttaaa caaaaatgaa
     4261 gttagttatg ttagtttttt gctgctttgg tgttacacct atttgaggtg gatacattcc
     4321 aagacccccc ctgtagatgc ctgaaactgt agattgtatc tagtcctata tatgttgttt
     4381 ttcctataca tatatactta aaagaaagtc tagtttatca attaggcaca gtaagagatc
     4441 agcagcaaca ataataaaac agaataatta taacaatatg ctgtaataaa agttttatga
     4501 atgta
//
