LOCUS       NM_001413676            4316 bp    mRNA    linear   PRI 15-MAR-2025
DEFINITION  Homo sapiens tumor suppressor candidate 3 (TUSC3), transcript
            variant 12, mRNA.
ACCESSION   NM_001413676
VERSION     NM_001413676.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Deng,R., Lu,X., Hong,C., Cai,R., Wang,P., Xiong,L., Wang,X.,
            Chen,Q. and Lin,J.
  TITLE     Downregulation of TUSC3 promotes EMT and hepatocellular carcinoma
            progression through LIPC/AKT axis
  JOURNAL   J Transl Med 20 (1), 485 (2022)
   PUBMED   36274132
  REMARK    GeneRIF: Downregulation of TUSC3 promotes EMT and hepatocellular
            carcinoma progression through LIPC/AKT axis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Sun,F., Jie,Q., Li,Q., Wei,Y., Li,H., Yue,X. and Ma,Y.
  TITLE     TUSC3 inhibits cell proliferation and invasion in cervical squamous
            cell carcinoma via suppression of the AKT signalling pathway
  JOURNAL   J Cell Mol Med 26 (5), 1629-1642 (2022)
   PUBMED   35137520
  REMARK    GeneRIF: TUSC3 inhibits cell proliferation and invasion in cervical
            squamous cell carcinoma via suppression of the AKT signalling
            pathway.
REFERENCE   3  (bases 1 to 4316)
  AUTHORS   Ren,Y., Deng,R., Cai,R., Lu,X., Luo,Y., Wang,Z., Zhu,Y., Yin,M.,
            Ding,Y. and Lin,J.
  TITLE     TUSC3 induces drug resistance and cellular stemness via Hedgehog
            signaling pathway in colorectal cancer
  JOURNAL   Carcinogenesis 41 (12), 1755-1766 (2020)
   PUBMED   32338281
  REMARK    GeneRIF: TUSC3 induces drug resistance and cellular stemness via
            Hedgehog signaling pathway in colorectal cancer.
REFERENCE   4  (bases 1 to 4316)
  AUTHORS   Ouidir,M., Mendola,P., Buck Louis,G.M., Kannan,K., Zhang,C. and
            Tekola-Ayele,F.
  TITLE     Concentrations of persistent organic pollutants in maternal plasma
            and epigenome-wide placental DNA methylation
  JOURNAL   Clin Epigenetics 12 (1), 103 (2020)
   PUBMED   32653021
  REMARK    GeneRIF: Concentrations of persistent organic pollutants in
            maternal plasma and epigenome-wide placental DNA methylation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4316)
  AUTHORS   Wang,S. and Zhu,W.
  TITLE     Tumour suppressor candidate 3 inhibits biological function and
            increases endoplasmic reticulum stress of melanoma cells WM451 by
            regulating AKT/GSK3-beta/beta-catenin pathway
  JOURNAL   Cell Biochem Funct 38 (5), 604-612 (2020)
   PUBMED   32090352
  REMARK    GeneRIF: Tumour suppressor candidate 3 inhibits biological function
            and increases endoplasmic reticulum stress of melanoma cells WM451
            by regulating AKT/GSK3-beta/beta-catenin pathway.
REFERENCE   6  (bases 1 to 4316)
  AUTHORS   Kelleher,D.J., Karaoglu,D., Mandon,E.C. and Gilmore,R.
  TITLE     Oligosaccharyltransferase isoforms that contain different catalytic
            STT3 subunits have distinct enzymatic properties
  JOURNAL   Mol Cell 12 (1), 101-111 (2003)
   PUBMED   12887896
REFERENCE   7  (bases 1 to 4316)
  AUTHORS   Ishii,H., Baffa,R., Numata,S.I., Murakumo,Y., Rattan,S., Inoue,H.,
            Mori,M., Fidanza,V., Alder,H. and Croce,C.M.
  TITLE     The FEZ1 gene at chromosome 8p22 encodes a leucine-zipper protein,
            and its expression is altered in multiple human tumors
  JOURNAL   Proc Natl Acad Sci U S A 96 (7), 3928-3933 (1999)
   PUBMED   10097140
REFERENCE   8  (bases 1 to 4316)
  AUTHORS   Pak,B.J., Park,H., Chang,E.R., Pang,S.C. and Graham,C.H.
  TITLE     Differential display analysis of oxygen-mediated changes in gene
            expression in first trimester human trophoblast cells
  JOURNAL   Placenta 19 (7), 483-488 (1998)
   PUBMED   9778121
REFERENCE   9  (bases 1 to 4316)
  AUTHORS   MacGrogan,D., Levy,A., Bova,G.S., Isaacs,W.B. and Bookstein,R.
  TITLE     Structure and methylation-associated silencing of a gene within a
            homozygously deleted region of human chromosome band 8p22
  JOURNAL   Genomics 35 (1), 55-65 (1996)
   PUBMED   8661104
REFERENCE   10 (bases 1 to 4316)
  AUTHORS   Sparks,S.E. and Krasnewich,D.M.
  TITLE     Congenital Disorders of N-Linked Glycosylation and Multiple Pathway
            Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301507
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC091559.7, AC010656.7 and
            AC019292.10.
            
            Summary: This gene encodes a protein that has been associated with
            several biological functions including cellular magnesium uptake,
            protein glycosylation and embryonic development. This protein
            localizes to the endoplasmic reticulum and acts as a component of
            the oligosaccharyl transferase complex which is responsible for
            N-linked protein glycosylation. This gene is a candidate tumor
            suppressor gene. Homozygous mutations in this gene are associated
            with autosomal recessive nonsyndromic mental retardation-7 and in
            the proliferation and invasiveness of several cancers including
            metastatic pancreatic cancer, ovarian cancer and glioblastoma
            multiform. [provided by RefSeq, Oct 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.1084238.1,
                                           SRR14038197.1823711.1 [ECO:0000332]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-306               AC091559.7         162788-163093
            307-476             AC010656.7         58176-58345         c
            477-594             AC010656.7         30611-30728         c
            595-735             AC010656.7         21778-21918         c
            736-876             AC010656.7         19129-19269         c
            877-966             AC010656.7         7589-7678           c
            967-4316            AC019292.10        44381-47730
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p22"
     gene            1..4316
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="tumor suppressor candidate 3"
                     /db_xref="GeneID:7991"
                     /db_xref="HGNC:HGNC:30242"
                     /db_xref="MIM:601385"
     exon            1..306
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             169..978
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="isoform 9 precursor is encoded by transcript
                     variant 12; oligosaccharyltransferase 3 homolog A;
                     magnesium uptake/transporter TUSC3; putative prostate
                     cancer tumor suppressor; oligosaccharyl transferase
                     subunit TUSC3; dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit TUSC3"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit TUSC3 isoform 9 precursor"
                     /protein_id="NP_001400605.1"
                     /db_xref="GeneID:7991"
                     /db_xref="HGNC:HGNC:30242"
                     /db_xref="MIM:601385"
                     /translation="MGARGAPSRRRQAGRRLRYLPTGSFPFLLLLLLLCIQLGGGQKK
                     KENLLAEKVEQLMEWSSRRSIFRMNGDKFRKFIKAPPRNYSMIVMFTALQPQRQCSVC
                     RQANEEYQILANSWRYSSAFCNKLFFSMVDYDEGTDVFQQLNMNSAPTFMHFPPKGRP
                     KRADTFDLQRIGFAAEQLAKWIADRTDVHIRVFRPPNYSGTIALALLVSLVGGLLYLR
                     RNNLEFIYNKTGWAMVSLCIVFAMTSGQMWNHIRGPPYAHKNPHNGQVLFN"
     exon            307..476
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            477..594
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            595..735
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            736..876
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            877..966
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     exon            967..4316
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1227..1232
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: AATAAA"
     polyA_site      1250
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="major polyA site"
     regulatory      1386..1391
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: ATTAAA"
     polyA_site      1429
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     regulatory      2003..2008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: AATAAA"
     polyA_site      2037
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     regulatory      2651..2656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: ATTAAA"
     polyA_site      2672
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     regulatory      2729..2734
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: ATTAAA"
     polyA_site      2749
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     regulatory      3296..3301
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: TATAAA"
     polyA_site      3316
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
     regulatory      4294..4299
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
                     /note="hexamer: ATTAAA"
     polyA_site      4316
                     /gene="TUSC3"
                     /gene_synonym="D8S1992; M33; MagT2; MRT22; MRT7; N33;
                     OST3A; SLC58A2"
ORIGIN      
        1 agtctcctcc tctgcgtcct cggccgcggc ccgggtccct cgcaaagccg ctgccatccc
       61 ggagggccca gccagcgggc tcccggaggc tggccgggca ggcgtggtgc gcggtaggag
      121 ctgggcgcgc acggctaccg cgcgtggagg agacactgcc ctgccgcgat gggggcccgg
      181 ggcgctcctt cacgccgtag gcaagcgggg cggcggctgc ggtacctgcc caccgggagc
      241 tttcccttcc ttctcctgct gctgctgctc tgcatccagc tcgggggagg acagaagaaa
      301 aaggagaatc ttttagctga aaaagtagag cagctgatgg aatggagttc cagacgctca
      361 atcttccgaa tgaatggtga taaattccga aaatttataa aggcaccacc tcgaaactat
      421 tccatgattg ttatgttcac tgctcttcag cctcagcggc agtgttctgt gtgcaggcaa
      481 gctaatgaag aatatcaaat actggcgaac tcctggcgct attcatctgc tttttgtaac
      541 aagctcttct tcagtatggt ggactatgat gaggggacag acgtttttca gcagctcaac
      601 atgaactctg ctcctacatt catgcatttt cctccaaaag gcagacctaa gagagctgat
      661 acttttgacc tccaaagaat tggatttgca gctgagcaac tagcaaagtg gattgctgac
      721 agaacggatg ttcatattcg ggttttcaga ccacccaact actctggtac cattgctttg
      781 gccctgttag tgtcgcttgt tggaggtttg ctttatttga gaaggaacaa cttggagttc
      841 atctataaca agactggttg ggccatggtg tctctgtgta tagtctttgc tatgacttct
      901 ggccagatgt ggaaccatat ccgtggacct ccatatgctc ataagaaccc acacaatgga
      961 caagtgcttt ttaattaaat gaagccaagt gggatttgca taaagtgaat gtttaccatg
     1021 aagataaact gttcctgact ttatactatt ttgaattcat tcatttcatt gtgatcagct
     1081 agcttattct tgtgtacttt ttttaaactg tgggttttcc tagtaaattt aatttacaga
     1141 aatcaatggt agcatttagt aatctacaaa ggaaatatca aagtgttttt caagcctgtt
     1201 atattcagtg tgtgccacag gattgaaata aatgacaatg taattatgaa ttcatgtttt
     1261 agagctgttt actcattagt aaaggaccgc aatgttagta aagaaaacct atgaaatgta
     1321 tgttaaagat tcttagtatt gtacagggat aaagcaaatg catgaaaata tgtcatgtac
     1381 tgaaaattaa acttacttca gtttcagtta taaaggacgt gcactgttac gaatagtgtt
     1441 ttgttattaa ttataactag ataaaggtat ataggacagg gaactggatg aatggtacct
     1501 acaatttgga tgcctaacca aggactagag ctccttcttg agatctaaat ctaaagtaaa
     1561 tgtgcattaa agcagtgtgc ttcaaaggca tcagacgatg aaagcaacat accacaacta
     1621 ggagttattt ctcaaactta aatgtcctct gggaatccag acttaaaaat aagagcaaac
     1681 ttaacacact atccattttc gagcaaacct aacccactat atccattttg ctcatgtgtt
     1741 ttatgcaacc agctttccat caaatcctca atctttgaat ccaggtaaaa ggttaattat
     1801 cctaggatta gtgaatgatt caatgaagct ttcttgaaaa caaacatagg agtgtaatgt
     1861 actattatgt ttgtatcctg ttttagttta taaagcactt tcacatacat tatggtattt
     1921 cttcctcaca ataaccttgt aaattagtca ggaaatgtaa attttatctg actcatagaa
     1981 ctaatggaag ctgaaagcca aaaataaagt ttataaccag gccttcaaac tctcaaacat
     2041 ggatttttat attctgtgga gttatccaaa ttaattaggg tttaggaaag cagttcttct
     2101 tgagtcagtt caataagtgt ttgctttttg tttttaaggt ttgttatcta attttcatta
     2161 agatttgagt aacagaccat ggagaattgt tttcattggg agttccagct atatagcctc
     2221 aaacaagaaa cgggtgtaca accattgagt ttacttactt aaatctgtga atttcaatga
     2281 agaatgggaa tataaaggcc tactggggca tgtttatcaa gttatatccc agggcaatgt
     2341 gtatgctagc aggatgaatg ctatcttttc aaaacataat attcagtgaa aattacgtaa
     2401 taattgtaag tttataatca tactcccaaa tctgttacta aaaataacat aaattctcct
     2461 agttcatgta cttagatgaa taaatttagt tttggaatga cgtttatgga aaagcaatag
     2521 atgaacttaa tcatttgatc actgcctatc actaggcaga acttgacctg taattcttaa
     2581 tccattgtag attttttaca ttttactcaa aacttcatac atagagactc tcaggtcaaa
     2641 ttttacaagt attaaacatt tgttaattat aatcactttt gtttgtatcc ttaaatctca
     2701 gaattatctt gcagttaata tgcagctgat taaatgatat tacaaattat ctctaatctc
     2761 actgtaaatc ttttaatcat atcaaagtca ggtcatcctc agacactata actaagatag
     2821 ataaggagta ctttactata ccatattagt aaaggttttc tagctcttat tttctaattt
     2881 cctctgagca tttaaaatta catccagtga taaaaacaat attttttttt cagaatttca
     2941 tgctttaaaa agctgtggct tctctataga caactgttac attagggaag tgattctaga
     3001 gcaaaatata ctgcctcaac ataagtcgtt actgactgga aaatgctata ggcagaattg
     3061 caaagagtgg ctcaaacata tagtttgctt gcagaatgta acgtgcaaaa tcacatacat
     3121 gcgatgtatt tacctttgct agacaatgag aattgaataa ttgatttgta cctggtatgt
     3181 tatatcctcc agtgtcactt tttaaccaga ttcactgtgc gttccaaact gtcataaaaa
     3241 ttgattatat gtttaacaat tgtttttcta ctacagtgga gagaaaaatc ccaattataa
     3301 aattccacct aaaggagttt cattttgtaa taacatctga ttaataactt gttgcatttt
     3361 gcttaacata ctgttcctgg cattagatat tcacatgagt aagcccagta gcattttgtg
     3421 cttactgcag ttttctttta cacacgaagt aacatccctt gtcaattaca attcagcagg
     3481 catggtgatt tggggagacg actagatgtt gctgtaaccg tagagcgtgg caagtattcc
     3541 ctttcttgct atcacctaag caaacctccc taagggtacg tgaagggagt ggttattcta
     3601 aataaggcat gacaaattac ttttcaacca gtgacattaa acattgtgtt ttaaaaagga
     3661 ctggtcactg gggcgaacac attagctgga gcatgtaggg acaaaatact tgaattgacg
     3721 cttgaagact caatgttctc aaagtccaca gagctcatta agtttaaaat agtaaaacaa
     3781 ggtcttcttc atactccagc aatatgctat attggatcag accaatatat gtaataatgt
     3841 tttctctaat agtaggaaaa ggatgagcat tgtagctagt cacactcaaa gggtacaagt
     3901 atgcattcat tagctcctaa attccctgat gatataattc acgagcatct gccatttatg
     3961 aattttcgat attcctctat gtctgaaata catgcatata tttatatcat ataccaaggg
     4021 tccaactact atacaaagat ttaacaaagc atctcacctt ccggccataa taggaaaatc
     4081 atgtcacatt tacccaaatg tggatacatc tatctatttc cattaaaaat tatgcataga
     4141 tgtcaggaaa aatgacagca aggaattcca gggacatacc tgtccttaga aaaacaggaa
     4201 aaaaaaaaaa cactgtcaca ttccatttta ttggaaattt gttttaaaaa aaaaagttta
     4261 ccaaaccact gtttaaaaca agaaaatgct taaattaaaa aagcaactga aactca
//
