LOCUS       NM_020461               6066 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens tubulin gamma complex component 6 (TUBGCP6), mRNA.
ACCESSION   NM_020461
VERSION     NM_020461.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Thomas-Wilson,A., Schacht,J.P., Chitayat,D., Blaser,S.,
            Santos,F.J.R., Glaser,K., Caffo,A., Wentzensen,I.M.,
            Henderson,L.B., Zhang,F., Zhu,Y., Di Corleto,E., da Silva Costa,F.,
            Vink,R., Alkhunaizi,E., Russell,L., Buckley,M.F., Roscioli,T.,
            Pereira,E.M. and Ganapathi,M.
  TITLE     Biallelic variants in TUBGCP6 result in microcephaly and
            chorioretinopathy 1: Report of four cases and a literature review
  JOURNAL   Am J Med Genet A 191 (7), 1935-1941 (2023)
   PUBMED   37031378
  REMARK    GeneRIF: Biallelic variants in TUBGCP6 result in microcephaly and
            chorioretinopathy 1: Report of four cases and a literature review.
            Review article
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Kato,K., Udagawa,R., Hayashi,Y., Maki,M., Yanagida,M.,
            Higashiura,S., Yagishita,R., Shimamoto,H. and Kimura,K.
  TITLE     SF3B14 is involved in the centrosome regulation through splicing of
            TUBGCP6 pre-mRNA
  JOURNAL   Biochem Biophys Res Commun 588, 133-139 (2022)
   PUBMED   34954520
  REMARK    GeneRIF: SF3B14 is involved in the centrosome regulation through
            splicing of TUBGCP6 pre-mRNA.
REFERENCE   3  (bases 1 to 6066)
  AUTHORS   Shurygina,M.F., Simonett,J.M., Parker,M.A., Mitchell,A.,
            Grigorian,F., Lifton,J., Nagiel,A., Shpak,A.A., Dadali,E.L.,
            Mishina,I.A., Weleber,R.G., Yang,P. and Pennesi,M.E.
  TITLE     Genotype Phenotype Correlation and Variability in Microcephaly
            Associated With Chorioretinopathy or Familial Exudative
            Vitreoretinopathy
  JOURNAL   Invest Ophthalmol Vis Sci 61 (13), 2 (2020)
   PUBMED   33137195
  REMARK    GeneRIF: Genotype Phenotype Correlation and Variability in
            Microcephaly Associated With Chorioretinopathy or Familial
            Exudative Vitreoretinopathy.
REFERENCE   4  (bases 1 to 6066)
  AUTHORS   Hull,S., Arno,G., Ostergaard,P., Pontikos,N., Robson,A.G.,
            Webster,A.R., Hogg,C.R., Wright,G.A., Henderson,R.H.H.,
            Martin,C.A., Jackson,A.P., Mansour,S., Moore,A.T. and
            Michaelides,M.
  TITLE     Clinical and Molecular Characterization of Familial Exudative
            Vitreoretinopathy Associated With Microcephaly
  JOURNAL   Am J Ophthalmol 207, 87-98 (2019)
   PUBMED   31077665
  REMARK    GeneRIF: a family of 2 children with learning difficulties,
            abnormal peripheral retinal vasculogenesis, and rod-cone dystrophy
            were investigated. They were found to have bi-allelic splicing
            variants in TUBGCP6.
REFERENCE   5  (bases 1 to 6066)
  AUTHORS   Navarini,A.A., Simpson,M.A., Weale,M., Knight,J., Carlavan,I.,
            Reiniche,P., Burden,D.A., Layton,A., Bataille,V., Allen,M.,
            Pleass,R., Pink,A., Creamer,D., English,J., Munn,S., Walton,S.,
            Willis,C., Deret,S., Voegel,J.J., Spector,T., Smith,C.H.,
            Trembath,R.C. and Barker,J.N.
  CONSRTM   Acne Genetic Study Group
  TITLE     Genome-wide association study identifies three novel susceptibility
            loci for severe Acne vulgaris
  JOURNAL   Nat Commun 5, 4020 (2014)
   PUBMED   24927181
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6066)
  AUTHORS   Takahashi,M., Yamagiwa,A., Nishimura,T., Mukai,H. and Ono,Y.
  TITLE     Centrosomal proteins CG-NAP and kendrin provide microtubule
            nucleation sites by anchoring gamma-tubulin ring complex
  JOURNAL   Mol Biol Cell 13 (9), 3235-3245 (2002)
   PUBMED   12221128
REFERENCE   7  (bases 1 to 6066)
  AUTHORS   Haren,L. and Merdes,A.
  TITLE     Direct binding of NuMA to tubulin is mediated by a novel sequence
            motif in the tail domain that bundles and stabilizes microtubules
  JOURNAL   J Cell Sci 115 (Pt 9), 1815-1824 (2002)
   PUBMED   11956313
REFERENCE   8  (bases 1 to 6066)
  AUTHORS   Murphy,S.M., Preble,A.M., Patel,U.K., O'Connell,K.L., Dias,D.P.,
            Moritz,M., Agard,D., Stults,J.T. and Stearns,T.
  TITLE     GCP5 and GCP6: two new members of the human gamma-tubulin complex
  JOURNAL   Mol Biol Cell 12 (11), 3340-3352 (2001)
   PUBMED   11694571
REFERENCE   9  (bases 1 to 6066)
  AUTHORS   Dunham,I., Shimizu,N., Roe,B.A., Chissoe,S., Hunt,A.R.,
            Collins,J.E., Bruskiewich,R., Beare,D.M., Clamp,M., Smink,L.J.,
            Ainscough,R., Almeida,J.P., Babbage,A., Bagguley,C., Bailey,J.,
            Barlow,K., Bates,K.N., Beasley,O., Bird,C.P., Blakey,S.,
            Bridgeman,A.M., Buck,D., Burgess,J., Burrill,W.D., O'Brien,K.P. et
            al.
  TITLE     The DNA sequence of human chromosome 22
  JOURNAL   Nature 402 (6761), 489-495 (1999)
   PUBMED   10591208
  REMARK    Erratum:[Nature 2000 Apr 20;404(6780):904]
REFERENCE   10 (bases 1 to 6066)
  AUTHORS   Hsu,H.L. and Yeh,N.H.
  TITLE     Dynamic changes of NuMA during the cell cycle and possible
            appearance of a truncated form of NuMA during apoptosis
  JOURNAL   J Cell Sci 109 (Pt 2), 277-288 (1996)
   PUBMED   8838651
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL022328.21.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_020461.3.
            
            Summary: The protein encoded by this gene is part of a large
            multisubunit complex required for microtubule nucleation at the
            centrosome. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF272887.1, SRR7346977.2859410.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000248846.10/ ENSP00000248846.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1305              AL022328.21        143874-145178       c
            1306-1469           AL022328.21        140359-140522       c
            1470-1680           AL022328.21        133471-133681       c
            1681-1854           AL022328.21        129559-129732       c
            1855-1976           AL022328.21        128062-128183       c
            1977-2055           AL022328.21        127154-127232       c
            2056-2165           AL022328.21        126888-126997       c
            2166-2257           AL022328.21        126442-126533       c
            2258-2397           AL022328.21        126205-126344       c
            2398-2547           AL022328.21        125949-126098       c
            2548-2629           AL022328.21        124666-124747       c
            2630-2718           AL022328.21        124487-124575       c
            2719-2834           AL022328.21        124296-124411       c
            2835-2973           AL022328.21        122609-122747       c
            2974-3048           AL022328.21        122183-122257       c
            3049-4672           AL022328.21        120406-122029       c
            4673-4731           AL022328.21        120112-120170       c
            4732-4879           AL022328.21        119799-119946       c
            4880-5048           AL022328.21        119443-119611       c
            5049-5190           AL022328.21        119223-119364       c
            5191-5385           AL022328.21        118858-119052       c
            5386-5518           AL022328.21        118643-118775       c
            5519-5732           AL022328.21        118344-118557       c
            5733-5932           AL022328.21        118073-118272       c
            5933-6066           AL022328.21        117849-117982       c
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.33"
     gene            1..6066
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /note="tubulin gamma complex component 6"
                     /db_xref="GeneID:85378"
                     /db_xref="HGNC:HGNC:18127"
                     /db_xref="MIM:610053"
     exon            1..1305
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     CDS             565..6024
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /note="tubulin gamma complex associated protein 6"
                     /codon_start=1
                     /product="gamma-tubulin complex component 6"
                     /protein_id="NP_065194.3"
                     /db_xref="CCDS:CCDS14087.1"
                     /db_xref="GeneID:85378"
                     /db_xref="HGNC:HGNC:18127"
                     /db_xref="MIM:610053"
                     /translation="MASITQLFDDLCEALLPAAKTHLGQRSVNRKRAKRSLKKVAYNA
                     LFTNLFQDETQQLQPDMSKLPARNKILMLSFDLRVGGLGPKADRLEELVEELEAAPCC
                     PLLEVGSVLDLLVQLAGSGPPQVLPRKRDYFLNNKHVGRNVPYSGYDCDDLSVFEMDV
                     QSLISREECLCHSMIQETLQVMEAAPGTGLPTVGLFSFGDPCGDRFERDTRVSLFGAL
                     VHSRTYDMDVRLGLPPVPDNADLSGLAIKVPPSVDQWEDEGFQSASNLTPDSQSEPSV
                     TPDVDLWEAALTYEASKRRCWERVGCPPGHREEPYLTEAGRDAFDKFCRLHQGELQLL
                     AGGVLQAPQPVLVKECELVKDVLNVLIGVVSATFSLCQPAQAFVVKRGVHVSGASPES
                     ISSLLSEVAEYGTCYTRLSHFSLQPVLDSLYSKGLVFQAFTSGLRRYLQYYRACVLST
                     PPTLSLLTIGFLFKKLGRQLRYLAELCGVGAVLPGTCGGGPRAAFPTGVKLLSYLYQE
                     ALHNCSNEHYPVLLSLLKTSCEPYTRFIHDWVYSGVFRDAYGEFMIQVNHEYLSFRDK
                     LYWTHGYVLISKEVEDCVPVFLKHIAHDIYVCGKTINLLKLCCPRHYLCWSDVPVPRI
                     SVIFSLEELKEIEKDCAVYVGRMERVARHSSVSKEEKELRMEIAKQELIAHAREAASR
                     VLSALSDRQMSERMALDARKREQFQRLKEQFVKDQERRQAARQEELDDDFSYARELRD
                     RERRLKSLEEELERKARQALVDHYSKLSAEAARREQKALWRIQRHRLESARLRFLLED
                     EKHIQEMLKAVSEAHQPQEPPDVLLSVHPQVTSPGPEHPEGGQGCDSGSAEQHSPAWD
                     GWNRPGLLTPQPLKPLAVGAGGRGLQQAEGARPFSDSLSIGDFLPVGPGAEPSVQTGM
                     VPLLEVALQTINLDLPPSAPGEAPAAASTQPSRPQEYDFSTVLRPAVATSPAPGPLQA
                     AECSLGSSGLQLWEDSCGKMDACGSASRETLLPSHPPRRAALEEGSSQPTERLFGQVS
                     GGGLPTGDYASEIAPTRPRWNTHGHVSDASIRVGENVSDVAPTQPRWNTHGHVSNASI
                     SLGESVSDVAPTRPRWNIHGHVSNASIRVGENVSDVAPTRPRWNTHGHVSNASIRVGE
                     NVSDVAPTRPRWNTHGHVSDASISLGESVSDMAPARPRWNTHGHVSDASISLGESVSD
                     MAPTRPRWNTHGHVSDTSIRVGENVSDVAPIRSRCNTHGHVSDASISLGEPVSDVVST
                     RPRWNTHVPIPPPHMVLGALSPEAEPNTPRPQQSPPGHTSQSALSLGAQSTVLDCGPR
                     LPVEVGPSLSSPSSGCGEGSISVGENVSDVAPTQPWWPNTPGDSVSEELGPGRSGDTE
                     DLSPNWPLNSQEDTAAQSSPGRGEEAEASAAEAQGGEQAYLAGLAGQYHLERYPDSYE
                     SMSEPPIAHLLRPVLPRAFAFPVDPQVQSAADETAVQLSELLTLPVLMKRSITAPLAA
                     HISLVNKAAVDYFFVELHLEAHYEALRHFLLMEDGEFAQSLSDLLFEKLGAGQTPGEL
                     LNPLVLNSVLSKALQCSLHGDTPHASNLSLALKYLPEVFAPNAPDVLSCLELRYKVDW
                     PLNIVITEGCVSKYSGVFSFLLQLKLMMWALKDVCFHLKRTALLSHMAGSVQFRQLQL
                     FKHEMQHFVKVIQGYIANQILHVTWCEFRARLATVGDLEEIQRAHAEYLHKAVFRGLL
                     TEKAAPVMNVIHSIFSLVLKFRSQLISQAWGPPGGPRGAEHPNFALMQQSYNTFKYYS
                     HFLFKVVTKLVNRGYQPHLEDFLLRINFNNYYQDA"
     exon            1306..1469
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1680
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1854
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1976
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2055
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..2165
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            2166..2257
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            2258..2397
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..2547
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            2548..2629
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            2630..2718
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            2719..2834
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..2973
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            2974..3048
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            3049..4672
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            4673..4731
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            4732..4879
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            4880..5048
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            5049..5190
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            5191..5385
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            5386..5518
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            5519..5732
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            5733..5932
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     exon            5933..6066
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6049..6054
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /note="hexamer: AATAAA"
     polyA_site      6066
                     /gene="TUBGCP6"
                     /gene_synonym="GCP-6; GCP6; MCCRP; MCCRP1; MCPHCR"
                     /note="major polyA site"
ORIGIN      
        1 acactggcgc ggctgtgacg cgacggaggc ggcgagagcg gcagccgtgg gcactttcca
       61 cgggtgaggc ctggagaacg ggtggacgtg caggccagaa cccgggaccc acccggctga
      121 gcccccagac tctccgctgc ccacgaccct cggagtggca ccctctcctg gccgtctcct
      181 ctcgtgggtc ccgaagaacg ggtgcaaagc cgagttcgtc cgctgtactg cgcgggcgcg
      241 cgtttcgttc ttcggttttg ccacggttct gtgactccct aaaggttgag ggctactgaa
      301 ttagaagaaa cgccgagctg gcttctggtg ggctgccggg cgccgggcag cgtgcttgga
      361 ctggggaggg cagggcgtgt ttactctgag tagagcaacc gcaagacctc ctttaaaaaa
      421 taccagttct gagcttcggc attggagagg ccgcctgtca ttcagcctct tcggaccctc
      481 tctcactccc tgtcttccgg agcgtgagtg aagcccgggt gaggtgtttt cccacacgcc
      541 gggggcccgg agctgagaag gggcatggcc agcatcacgc agctgttcga cgacctgtgt
      601 gaggccctcc tgccggctgc caagactcac ctgggccagc gcagtgtgaa ccggaagagg
      661 gcaaagcgga gcctcaagaa ggtggcctac aatgctcttt tcacaaatct ttttcaagat
      721 gagactcaac agctgcagcc tgacatgtca aaactaccag cgagaaacaa gatcctcatg
      781 ttgtcctttg acttgagagt gggtggcctg ggccccaagg ccgaccgttt ggaggagctt
      841 gtggaggagc tggaagcagc cccttgctgt ccgcttttgg aggtggggtc tgttttggac
      901 ctcctggttc agctggcagg gagtggtccc cctcaagttc tgccgagaaa acgagactac
      961 ttccttaaca acaagcatgt ggggagaaac gttccgtaca gcggctatga ttgcgacgac
     1021 ctgagtgtgt ttgagatgga cgttcagtct ctgatctcca gagaagagtg tttgtgtcac
     1081 agcatgatcc aggaaacact tcaggttatg gaggctgctc caggcactgg cctgcccacc
     1141 gtcgggctct tctcatttgg tgacccttgt ggtgacaggt tcgagagaga cacccgggtc
     1201 tcgctcttcg gggcccttgt gcacagccgc acttatgaca tggacgtccg actgggcctg
     1261 ccccccgtgc cagacaatgc ggacctctct gggctggcta ttaaggtccc accgagtgtg
     1321 gatcagtggg aagacgaagg attccagtca gcgtccaact tgactcctga ttcccagtct
     1381 gagcccagcg tgaccccaga cgtggacctg tgggaagccg cacttaccta tgaggccagc
     1441 aagcggaggt gctgggagcg agttggctgc ccccctggcc acagagagga gccttacctg
     1501 acggaggcgg gaagggacgc tttcgacaag ttctgcaggc tccaccaagg ggagctccag
     1561 ctgcttgctg ggggcgtcct acaggccccg cagcccgtgc tggtgaagga gtgcgagctg
     1621 gtgaaagacg tgctgaacgt cttgattggg gtcgtgtctg ccacgttttc gctctgccag
     1681 ccggcccagg cctttgtggt gaagcggggc gtccacgtgt caggagcgtc tcccgagagc
     1741 atcagcagcc tgctctcgga agtggccgag tatgggacct gctacacgcg cctgagtcat
     1801 ttctctctgc agcccgtcct ggactctttg tacagcaagg gcctcgtgtt ccaggccttc
     1861 accagtggcc tgaggaggta cctgcagtat taccgggcct gcgtcctttc cactccgccc
     1921 accctgagcc tcctcaccat tggttttctc ttcaagaaac ttggccggca gctcaggtac
     1981 ctggccgagc tctgtggcgt tggcgctgtg ctcccgggca cctgtggagg aggccccagg
     2041 gccgcgtttc ccaccggcgt gaagctgctg tcctacctct accaggaggc tctgcacaac
     2101 tgcagcaacg agcactaccc tgtactgctg tccctgctga agaccagctg cgagccctac
     2161 acccggttca tccacgactg ggtgtacagc ggggtgttca gagacgctta tggcgagttc
     2221 atgattcagg tgaaccacga gtacctcagc ttccgagata agttgtactg gacacatggc
     2281 tacgtgctca tctccaaaga ggtggaggac tgtgttcccg tgtttctgaa gcacattgcc
     2341 cacgacatat acgtctgcgg aaagaccatt aacctgctga agctctgctg cccccggcat
     2401 tacctctgtt ggtccgacgt ccctgtcccc cggatctcgg tgattttctc ccttgaggag
     2461 ttgaaggaga ttgagaagga ctgtgccgtc tacgttgggc gcatggagag ggtggcccgc
     2521 cacagctctg tcagcaagga ggagaaggaa ttacgtatgg aaattgcaaa acaagaatta
     2581 atcgctcatg cccgggaagc agcatccagg gtcctgagtg cactgagtga ccggcagatg
     2641 tcagaacgga tggccttgga tgcccggaag cgtgagcagt ttcagaggct gaaagaacaa
     2701 tttgtgaagg accaggagcg acgccaggcg gccaggcagg aggagctgga tgatgacttc
     2761 agctacgccc gtgaactccg agacagggag agaaggctga agtccctgga ggaggagctg
     2821 gagaggaagg cgaggcaggc actggtcgac cactacagca agctctctgc agaggcagct
     2881 cgtcgggagc agaaggcact gtggagaatc cagaggcacc gactggagag tgcacggctt
     2941 cgttttctct tagaagatga gaaacacatt caggagatgc tgaaagcagt gtctgaggct
     3001 caccagcccc aggagccgcc agacgtcctc ttgagcgtgc acccccaggt cacgtctccg
     3061 ggccctgagc acccagaggg aggccaaggc tgtgattctg ggtctgcaga gcaacactcg
     3121 cctgcctggg atggctggaa caggccaggc ctgctgaccc cacagcccct taagcctcta
     3181 gcagtggggg ctggtggcag ggggctgcag caggcggagg gggccagacc cttctctgac
     3241 agcctcagca ttggagactt cctacctgtg ggcccagggg ctgagccgtc cgtgcagact
     3301 ggcatggtcc ctctcctgga ggtggcgctg cagaccatta acttggacct gcccccctca
     3361 gctcctgggg aggcacccgc agcagccagc actcagccct ccaggccaca ggagtacgac
     3421 tttagtactg tcctgaggcc agctgtggcc acctcacctg caccagggcc cctgcaggct
     3481 gcagagtgca gcttgggcag ctcagggcta cagctgtggg aggacagttg tgggaagatg
     3541 gatgcctgtg gctccgcctc gcgggagact ctgctcccct cacacccacc caggcgtgct
     3601 gctctggagg aggggagcag ccagcccaca gagcggctct ttgggcaggt gtcagggggt
     3661 ggtcttccca caggggacta cgcttctgaa atagctccca cccggccacg gtggaacacc
     3721 cacgggcacg tgtctgacgc cagcatcagg gtcggggaga atgtgtcaga tgtggctccc
     3781 acccagccac ggtggaacac ccacggacac gtatccaatg ccagcatcag cttaggggag
     3841 tctgtgtcag atgtggctcc cactcggcca cggtggaaca tccatggaca tgtgtccaac
     3901 gccagcatca gggtcgggga gaatgtgtca gatgtggctc ccaccaggcc acggtggaat
     3961 acccacgggc acgtgtccaa cgccagcatc agggttgggg agaacgtgtc ggacgtggct
     4021 cccacccggc cacggtggaa cacccatgga cacgtgtccg atgccagcat cagcttgggg
     4081 gagtctgtgt cagacatggc tcccgcccgg ccacggtgga acacccatgg acacgtgtct
     4141 gatgccagca tcagcttggg ggagtctgtg tcagacatgg ctcccacccg gccacggtgg
     4201 aacacccacg gacatgtgtc tgacaccagc atcagggttg gggagaacgt atcggacgtg
     4261 gctcccatcc ggtcacggtg caacacccat ggacacgtgt ccgacgccag catcagcttg
     4321 ggggagcctg tgtcggatgt ggtttccacc cggccacggt ggaacaccca tgtacccatc
     4381 cctccgcccc acatggtgct gggggctctc tcaccagaag ctgagcccaa cacacccagg
     4441 ccccaacaga gcccccctgg ccacacgtcc cagtcagcgc tcagcctggg agcacagagc
     4501 actgtgctgg actgtgggcc acggctgcct gtagaagtgg ggccatctct gtcctccccc
     4561 agctcgggct gcggggaggg gagcatcagc gtgggggaga acgtgtcaga cgtggctccc
     4621 acccaaccat ggtggcccaa caccccggga gacagtgtct ctgaagaact aggccctggg
     4681 aggagcgggg acactgagga cctctctcca aattggcctc tcaactcaca ggaagacaca
     4741 gctgcccaga gcagcccagg ccgtggtgag gaggcggagg catcggcggc ggaggctcag
     4801 ggtggggagc aggcctacct ggcaggcctg gcagggcagt accacttgga gcggtacccg
     4861 gacagttacg agtccatgtc cgagccgccc attgctcatc ttttgcgccc cgtgcttccc
     4921 cgggccttcg ccttccccgt ggacccccag gtccagtctg ccgctgatga gactgctgtg
     4981 cagctgagcg agttgctgac gctgcccgtg ctcatgaagc gctccatcac ggcaccgctg
     5041 gccgcccaca tctccttggt gaacaaggcc gctgtcgact acttcttcgt ggagctgcac
     5101 ctggaggcgc actatgaggc actgcggcac ttcctgctga tggaggacgg cgagttcgcc
     5161 cagtccctca gcgacctgct ctttgagaag cttggagctg ggcaaacgcc cggagagctg
     5221 ctcaacccgc tggtgctgaa ctctgtgctg agcaaggccc tgcagtgcag cctgcatggg
     5281 gacaccccgc acgcctccaa cctctccctc gctctcaagt acctgcccga ggtgtttgcc
     5341 cccaacgccc cggatgtgct gagctgcctg gagctcaggt acaaggtgga ctggcctctc
     5401 aacattgtca tcaccgaggg ctgcgtgagc aagtacagcg gcgtcttctc cttcctgctg
     5461 cagctgaagc tcatgatgtg ggcgctcaag gacgtctgct tccacctcaa gcgcacagcc
     5521 ctgctgagcc acatggccgg ctctgtgcag ttccgtcagc tgcagctgtt caagcacgag
     5581 atgcagcatt tcgtgaaggt catccagggc tacatcgcca accagatcct gcacgtcacc
     5641 tggtgcgagt tcagggccag gttggccacc gtgggcgacc tggaggagat ccagcgtgcg
     5701 cacgcagagt acctgcacaa ggccgtcttc aggggcctgc tcacggagaa ggcggcgccc
     5761 gtcatgaacg tcatccacag catcttcagc ctcgtgctca agttccgcag ccagctcatc
     5821 tcccaggcct gggggccccc tgggggcccg cggggtgcag agcaccccaa ctttgcactc
     5881 atgcagcagt cctacaacac cttcaagtac tactcccact ttctcttcaa agtggtgacc
     5941 aagctggtga accgcggcta ccagccccac ctggaggact ttctgctgcg catcaacttc
     6001 aacaactact accaggacgc ctgaggctgc tctgcggggg acgtgcacaa taaaggtgtt
     6061 ctcgga
//
