LOCUS       NM_014444               6812 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens tubulin gamma complex component 4 (TUBGCP4),
            transcript variant 2, mRNA.
ACCESSION   NM_014444
VERSION     NM_014444.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Yahalom,C., Woods,R.L., Akula,J.D., Tan,W.H. and Fulton,A.
  TITLE     Microcephaly and chorioretinopathy associated with TUBGCP4: a case
            report and a review of the literature
  JOURNAL   Ophthalmic Genet 44 (6), 585-590 (2023)
   PUBMED   37038737
  REMARK    GeneRIF: Microcephaly and chorioretinopathy associated with
            TUBGCP4: a case report and a review of the literature.
            Review article
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Chen,C., Liu,Y., Liu,L., Si,C., Xu,Y., Wu,X., Wang,C., Sun,Z. and
            Kang,Q.
  TITLE     Exosomal circTUBGCP4 promotes vascular endothelial cell tipping and
            colorectal cancer metastasis by activating Akt signaling pathway
  JOURNAL   J Exp Clin Cancer Res 42 (1), 46 (2023)
   PUBMED   36793126
  REMARK    GeneRIF: Exosomal circTUBGCP4 promotes vascular endothelial cell
            tipping and colorectal cancer metastasis by activating Akt
            signaling pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6812)
  AUTHORS   Zheng,C., Zhang,J., Jiang,F., Li,D., Huang,C., Guo,X., Zhu,X. and
            Tan,S.
  TITLE     Clinical Significance of TUBGCP4 Expression in Hepatocellular
            Carcinoma
  JOURNAL   Anal Cell Pathol (Amst) 2022, 9307468 (2022)
   PUBMED   36530949
  REMARK    GeneRIF: Clinical Significance of TUBGCP4 Expression in
            Hepatocellular Carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6812)
  AUTHORS   Shurygina,M.F., Simonett,J.M., Parker,M.A., Mitchell,A.,
            Grigorian,F., Lifton,J., Nagiel,A., Shpak,A.A., Dadali,E.L.,
            Mishina,I.A., Weleber,R.G., Yang,P. and Pennesi,M.E.
  TITLE     Genotype Phenotype Correlation and Variability in Microcephaly
            Associated With Chorioretinopathy or Familial Exudative
            Vitreoretinopathy
  JOURNAL   Invest Ophthalmol Vis Sci 61 (13), 2 (2020)
   PUBMED   33137195
  REMARK    GeneRIF: Genotype Phenotype Correlation and Variability in
            Microcephaly Associated With Chorioretinopathy or Familial
            Exudative Vitreoretinopathy.
REFERENCE   5  (bases 1 to 6812)
  AUTHORS   Scheidecker,S., Etard,C., Haren,L., Stoetzel,C., Hull,S., Arno,G.,
            Plagnol,V., Drunat,S., Passemard,S., Toutain,A., Obringer,C.,
            Koob,M., Geoffroy,V., Marion,V., Strahle,U., Ostergaard,P.,
            Verloes,A., Merdes,A., Moore,A.T. and Dollfus,H.
  TITLE     Mutations in TUBGCP4 alter microtubule organization via the
            gamma-tubulin ring complex in autosomal-recessive microcephaly with
            chorioretinopathy
  JOURNAL   Am J Hum Genet 96 (4), 666-674 (2015)
   PUBMED   25817018
  REMARK    GeneRIF: Mutations in TUBGCP4 alter microtubule organization via
            the gamma-tubulin ring complex in autosomal-recessive microcephaly
            with chorioretinopathy.
REFERENCE   6  (bases 1 to 6812)
  AUTHORS   Guillet,V., Knibiehler,M., Gregory-Pauron,L., Remy,M.H., Chemin,C.,
            Raynaud-Messina,B., Bon,C., Kollman,J.M., Agard,D.A., Merdes,A. and
            Mourey,L.
  TITLE     Crystal structure of gamma-tubulin complex protein GCP4 provides
            insight into microtubule nucleation
  JOURNAL   Nat Struct Mol Biol 18 (8), 915-919 (2011)
   PUBMED   21725292
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6812)
  AUTHORS   Haren,L. and Merdes,A.
  TITLE     Direct binding of NuMA to tubulin is mediated by a novel sequence
            motif in the tail domain that bundles and stabilizes microtubules
  JOURNAL   J Cell Sci 115 (Pt 9), 1815-1824 (2002)
   PUBMED   11956313
REFERENCE   8  (bases 1 to 6812)
  AUTHORS   Murphy,S.M., Preble,A.M., Patel,U.K., O'Connell,K.L., Dias,D.P.,
            Moritz,M., Agard,D., Stults,J.T. and Stearns,T.
  TITLE     GCP5 and GCP6: two new members of the human gamma-tubulin complex
  JOURNAL   Mol Biol Cell 12 (11), 3340-3352 (2001)
   PUBMED   11694571
REFERENCE   9  (bases 1 to 6812)
  AUTHORS   Fava,F., Raynaud-Messina,B., Leung-Tack,J., Mazzolini,L., Li,M.,
            Guillemot,J.C., Cachot,D., Tollon,Y., Ferrara,P. and Wright,M.
  TITLE     Human 76p: A new member of the gamma-tubulin-associated protein
            family
  JOURNAL   J Cell Biol 147 (4), 857-868 (1999)
   PUBMED   10562286
REFERENCE   10 (bases 1 to 6812)
  AUTHORS   Hsu,H.L. and Yeh,N.H.
  TITLE     Dynamic changes of NuMA during the cell cycle and possible
            appearance of a truncated form of NuMA during apoptosis
  JOURNAL   J Cell Sci 109 (Pt 2), 277-288 (1996)
   PUBMED   8838651
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA396753.1, BC012801.2,
            AC018924.9 and CK299820.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_014444.4.
            
            Summary: This gene encodes a component of the gamma-tubulin ring
            complex, which is required for microtubule nucleation. In mammalian
            cells, the protein localizes to centrosomes in association with
            gamma-tubulin. Crystal structure analysis revealed a structure
            composed of five helical bundles arranged around conserved
            hydrophobic cores. An exposed surface area located in the
            C-terminal domain is essential and sufficient for direct binding to
            gamma-tubulin. Mutations in this gene that alter microtubule
            organization are associated with microcephaly and
            chorioretinopathy. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, May 2015].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site compared to variant 1. The encoded isoform (b) is
            shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.2104735.1,
                                           SRR14038197.2325355.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000564079.6/ ENSP00000456648.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                DA396753.1         43-56
            15-3101             BC012801.2         1-3087
            3102-3544           AC018924.9         76394-76836
            3545-4136           CK299820.1         17-608              c
            4137-6812           AC018924.9         77429-80104
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.3"
     gene            1..6812
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /note="tubulin gamma complex component 4"
                     /db_xref="GeneID:27229"
                     /db_xref="HGNC:HGNC:16691"
                     /db_xref="MIM:609610"
     exon            1..332
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             255..2255
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /note="isoform b is encoded by transcript variant 2; gamma
                     tubulin ring complex protein (76p gene); gamma-tubulin
                     complex component 4; gamma-ring complex protein 76 kDa;
                     tubulin gamma complex associated protein 4; gamma-tubulin
                     complex protein 4"
                     /codon_start=1
                     /product="gamma-tubulin complex component 4 isoform b"
                     /protein_id="NP_055259.2"
                     /db_xref="CCDS:CCDS42030.1"
                     /db_xref="GeneID:27229"
                     /db_xref="HGNC:HGNC:16691"
                     /db_xref="MIM:609610"
                     /translation="MIHELLLALSGYPGSIFTWNKRSGLQVSQDFPFLHPSETSVLNR
                     LCRLGTDYIRFTEFIEQYTGHVQQQDHHPSQQGQGGLHGIYLRAFCTGLDSVLQPYRQ
                     ALLDLEQEFLGDPHLSISHVNYFLDQFQLLFPSVMVVVEQIKSQKIHGCQILETVYKH
                     SCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSGNVSAQP
                     EEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRVAEKI
                     LFVGESVQMFENQNVNLTRKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRS
                     TVAEHLWKLMVEESDLLGQLKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVTEHDV
                     NVAFQQSAHKVLLDDDNLLPLLHLTIEYHGKEHKDATQAREGPSRETSPREAPASGWA
                     ALGLSYKVQWPLHILFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKHLKSNQ
                     TDAIKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSN
                     LLAQSFILLKPVFHCLNEILDLCHSFCSLVSQNLGPLDERGAAQLSILVKGFSRQSSL
                     LFKILSSVRNHQINSDLAQLLLRLDYNKYYTQAGGTLGSFGM"
     exon            333..461
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            462..584
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            585..638
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            639..695
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            696..775
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            776..977
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1143
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1268
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1319
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1425
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1533
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1672
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1850
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1985
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2102
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2103..2242
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..6812
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4111..4116
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /note="hexamer: AATAAA"
     polyA_site      4136
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /note="major polyA site"
     regulatory      6787..6792
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
                     /note="hexamer: ATTAAA"
     polyA_site      6812
                     /gene="TUBGCP4"
                     /gene_synonym="76P; GCP-4; GCP4; Grip76; MCCRP3"
ORIGIN      
        1 agtctccgca gagcccgggc gggagtagct ggtggacccc gttgagctgc cgaacttccg
       61 ggactccccc gcgacccctt cccagcttcc cgtccgctcc gccgcagcga ttgtctcggt
      121 gggttgattc ggcacaaacc gcccgaccca ggggccggtg cgcgtgtgga aggggaagca
      181 ctcccctcgt ggtcgcctgg aggtgcgctg gaggaggggg tgacataacc agggactcga
      241 ggtccgccgt gggaatgatc cacgaactgc tcttggctct gagcgggtac cctgggtcca
      301 ttttcacctg gaacaagcgg agtggcctgc aggtatcgca ggacttccct ttcctccacc
      361 ccagtgagac cagtgtcctg aatcgactct gccggctcgg cacagactat attcgcttca
      421 ctgagttcat tgaacagtac acgggccatg tgcaacagca ggatcaccat ccatctcaac
      481 agggccaagg tgggttacat ggaatctacc tgcgggcctt ctgcacaggg ctggattctg
      541 ttttgcagcc ttatcgccaa gcactgcttg atttggaaca agagttcctg ggtgatcccc
      601 atctctccat atcacatgtc aactacttcc tagaccagtt ccagcttctt tttccctctg
      661 tgatggttgt agtagaacaa attaaaagtc aaaagattca tggttgtcaa atcctggaaa
      721 cagtctacaa acacagctgt ggggggttgc ctcctgttcg aagtgcactg gaaaaaatcc
      781 tggccgtttg tcatggggtc atgtataaac agctctcagc ctggatgctc catggactcc
      841 tcttggacca gcatgaagaa ttctttatca aacaggggcc atcttctggt aatgtcagtg
      901 cccagccaga agaggacgag gaggatctgg gcattggggg actgacagga aaacaactga
      961 gagaactgca ggacttgcgc ctgattgagg aagagaacat gctggcacca tctctgaagc
     1021 agttttccct acgagtggag attttgccat cctacattcc agtgagggtt gctgaaaaaa
     1081 tcctatttgt tggagaatct gtccagatgt ttgagaatca aaatgtgaac ctgactagaa
     1141 aaggatccat tttgaaaaac caggaagaca cttttgctgc agagctgcac cgtctcaagc
     1201 agcagccact cttcagcttg gtggactttg aacaggtggt ggatcgcatt cgcagcactg
     1261 tggctgagca tctctggaag ttgatggtag aagaatccga tttactgggt cagctgaaga
     1321 tcattaaaga cttttacctt ctgggacgtg gagaactgtt tcaggccttc attgacacag
     1381 ctcaacacat gttgaaaaca ccacccactg cagtaactga gcatgatgtg aatgtggcct
     1441 ttcaacagtc agcacacaag gtattgctag atgatgacaa ccttctccct ctgttgcact
     1501 tgacaatcga gtatcacgga aaggagcaca aagatgctac tcaggcaaga gaagggcctt
     1561 ctcgggaaac ttctccccgg gaagcccctg catctggctg ggcagcccta ggtctttcct
     1621 acaaagtaca gtggccacta catattctct tcaccccagc tgtcctggaa aagtacaatg
     1681 ttgtttttaa gtacttactg agtgtgcgcc gggtgcaagc tgagctgcag cactgctggg
     1741 ccctacaaat gcagcgcaag cacctcaagt cgaaccagac tgatgcaatc aagtggcgcc
     1801 taagaaatca catggcattt ttggtggata atcttcagta ctatctccag gtagatgtgt
     1861 tggagtctca gttctcccag ctgcttcatc agatcaattc tacccgagac tttgaaagca
     1921 tccgattggc tcatgaccac ttcctgagca atttgctggc tcaatccttt atcctattga
     1981 aacctgtgtt tcactgcctg aatgaaatcc tagatctctg tcacagtttt tgttcgctgg
     2041 tcagtcagaa cctaggccca ctggatgagc gtggagccgc ccagctgagc attctcgtga
     2101 agggctttag ccgccagtct tcactcctgt tcaagattct ctccagtgtt cggaatcatc
     2161 agatcaactc agatttggct caactactgt tacgactaga ttataacaaa tactataccc
     2221 aggctggtgg aactctgggc agtttcggga tgtgaaaatt tctggctcat aaattgaaat
     2281 aacagccacg ttcccaaggt tgtaacagaa gattcaaaac atcccattct agccacacac
     2341 aaataaatat ctgcggctta gtgataggac tctacctttt ctcctagaag cagttactga
     2401 acatccagga gtacaactcc ttcccatcat tcccatgtgg aagggtctct cccatcaagg
     2461 agaacatgtg gcatctctga tcctttacat tgagaacatt tgttggatat gttcatttat
     2521 tcaatagtca tttattgagc acctactacg taccttggta ctgttcaagc tgtgggagat
     2581 acagcggtaa acaaacaata tagagcagaa agttaaatat tttatggttc atatgtgaaa
     2641 aagtaattat gtttataaat agactaactg ctggatgtta ccaccaagta agaaagcaac
     2701 aggtaagata ggctttctct ctccctatac caagtaattt atacctacac agattgggca
     2761 attctagcta atgaaaatat acttaaaagt atttcttagg ccgggcatgg tggctcacac
     2821 ctgtaatccc agcactttgg gaggccgagg cgggcggatc acctgaagtc aggagtttga
     2881 gaccagcctg accaacatga tgaaacctcg attctactaa aaatacaaaa attagccagg
     2941 tgtggtggca tgtgcctgta atcccagcta ctcaggaggc tgagacagga gaattgcttg
     3001 aacctgggaa gcagacgctg cagtgagctg agattgtgcc attgcattcc agcccgggca
     3061 acaagagcga aattccgtct caaaaaaaaa aaaaaaaaaa aaaaaagtat tattctccaa
     3121 gaaaaaggtc cttaagaaaa aattgagatc aagttgttag atttttaaat actgaagatt
     3181 gcaggcccaa ttacccatct tacacaaacc ataggggttg aagttatctt aatatggccc
     3241 agccatcact ggtaatcaat attcatatca gtgtaagtaa aaagaaatat tcactgaaca
     3301 acgccctcca aactgaaaaa gaatgcagtg ttctggcatc aggttatagt cactgcatct
     3361 ggttttcatc actacatatt ctacacacac tgggaagctc tgacaactta ttccctgcta
     3421 ttatcaacta aagatcaccc tttctactgc tgtctctgga gcaggagctg gcaaactatg
     3481 gcctgctgtc tgtttttgta cagttttact gaaacacagc catgcccatt tgtttactca
     3541 ttgtctatgg ttgctttcat gccctcacag caaaggcgag tagttgtgat ggatcaaatg
     3601 gcccacaaag cctgaaatat ttactctttg accctttaca gaaaaaaacc ttgttgaccc
     3661 ctgctttaga gaatgagaag ccatgcaggg atcagtgatg ccagaggaag ggaaggaact
     3721 gcttccagct attgtgacaa taataataat aataatattg ggtctttgac tagaacgtgt
     3781 aacatttcca ggtgttctca cttgtgcttc ccatgtttat cttacggaag gtcattccat
     3841 caagcttatg gtcactgtcc cttcatggca gttggtcctt tcgttctccc tttagctcta
     3901 agagttgggg agtacccaca ggtgagctgt gatctcagct cagagagaga gcatgaggtc
     3961 ttttttaact gtcaggaaac agagctgtgc ccaattccac tcaacttttg gcacaactgt
     4021 taatctgggc cttcacctac cttaaactga gtttctgcaa gcatagcatt ttagacaccc
     4081 tggaataacc ttttgggaat gatgccacag aataaagttc actcttaact tttcaatttc
     4141 cttggccagc tgtcctccgt aagtgaataa gcctgttgaa agactcagag aaagtactat
     4201 gtcttgtcat ttgttctgag attaagctca aaaaaacaga tgaagaaatc ccagttacta
     4261 caaccaaaga gattcaacat ttattttatc ataaaagttc agcaaataaa actatataca
     4321 agatccatgc aaggaatcca gttacacaca agacacattt aaaacctggt taaaacacaa
     4381 tctccacgat agcagggaat aaaaccagta agaccaagta tctttagtga gaaacataat
     4441 cgtgtttata ttttggatgc tgcttgaatc caattctctc cccaacaatg aggcactgga
     4501 tcacccactc ttgtgacacc acaggcagct gcaatgcttc agcacacttc agcaccgagg
     4561 ctgggcatga ggggtccgtc accaccacat caaatacccc taaagcaata tctgcaagga
     4621 gcaagggaaa gtgaagaagg aaaggacact caacttagcc ctccattaga aagagagatt
     4681 tgattctaac caatacatcc cactctgcac aaaccaaagc cctattatgt caaacacact
     4741 gctactgatc atgaccaaag gcagagttat aatcactatg tgctgacctt gtagaaatat
     4801 ttaacaaata tacgtccagt gcttcactta tgttgactca cctcttgaag gtggtacttt
     4861 tcttctctaa gaaacatgga tacggtcaac ctattaggcc tgagccttgg accacaaggc
     4921 ctaacaccta caggtctaag gagatccctg gaacaaagac actacacaca ctctttcagg
     4981 tacctttgtt atgggcactt gaatggtgct gcttcacaga ggctgcacca ccagtcatga
     5041 ggatctcaga ccagagctcc aggaagttct gctgttggtc tgataccaag agtaccttca
     5101 gattctggaa aggattttca cggggttgcc tatgaaggag acaggaaagg accttagcat
     5161 gacaagtaat atccaacaaa ctgcctttct gcaaagggac tcatgtacat ctgaatgctt
     5221 tcaaaaataa atgccccatc agacatagtg tctcaagcct gtaatcccag cactttggga
     5281 ggctgtcgtg gttggatctc ttgggcctgg gagttcgaga ccagcctggg caatgtggtg
     5341 agaccccatc tctacaaaag acaacaaaaa aattagctgg gtgtggtggc gagtgcctgt
     5401 agtcccagca gcttgggagg ctgaggtagg gggatcactt cagcctggga ggttgaggct
     5461 gcagtaagtc gtcactgcgc cactgtactc cagcctaggt gacagagcaa gacttcatct
     5521 taaaaaacta agccctatat tagggtcccc cttctcttcc ttctttctat gaatgatctg
     5581 tattccttgc attcctggct ttctaatttc catgtttgtt ctggggctga gaataatcca
     5641 aatcatgctc ctgagcctat atatttttaa tgcttgctta aaacttagtt ctctgacttt
     5701 acaggttgag aatattgaac ctatatacaa atcttcacac atttgcaaaa ggttcctagc
     5761 caatgtaacc tagggaaata aactagataa actcctgaag tcatttcaaa cccactcaaa
     5821 tttatcccac agacattcca atttctagaa agctttactc tctcacctag attctcttcc
     5881 ctccaaagct tgctgtcctc ctgcctatac aattctggat gggcttcaaa tacttaccag
     5941 tccagaattc tttgctcctc aaggctgtac ccagctggca acagataatt acggtagttc
     6001 tggagctggt tggcatggca actatcatgg acccagacat gagacacaca aggaatccca
     6061 ctggcaaggc acaggaagta cttccgggtt cgacaatgct gatccgcaat tagaagacac
     6121 tggtaagctg tgttacactg caagaaaaga agcagagcca atgggtttgg tgacttctgt
     6181 ggaaagctcc taagcagcag ccataatgag ccatgaagag cagatctgaa gactcccaac
     6241 tactacccaa aatgtgattt agtctatcct gcccaaggcc actcttctca ctggaaggcc
     6301 caagtaattt ccatagatgt tctctctgcc tcacctgcag catactgagg acctaaatcc
     6361 tcaacggaca accaaaacct atgaactcag cctttcaggc taaaaatcag caaccctaat
     6421 aggggtttct actactaaac ataaacatca atcttctttt gtcccagcaa cagaaccata
     6481 gccattaact aacccaaggt cctaccttct cttccctata cacaacaaaa attctatttc
     6541 atgcaaaaac attttggcag tttctcagtt cctgaaatct ctggctactt tatccaggtt
     6601 ccccaacccc tcccaggcct cttctcaaca cagcaagttg gctcttatca ttgccactat
     6661 attaggttac acaaagaaac tcctcacctg ggcttcattg aaatcttcaa ggatatagcc
     6721 agctcctgct cgaagctggg attctgtata ctgcttgttg aaaggaggaa tttccaaaaa
     6781 ttctatatta aaaaaaaaaa ccaagataat aa
//
