LOCUS       NM_016437               1782 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens tubulin gamma 2 (TUBG2), transcript variant 2, mRNA.
ACCESSION   NM_016437
VERSION     NM_016437.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Draberova,E., Sulimenko,V., Vinopal,S., Sulimenko,T., Sladkova,V.,
            D'Agostino,L., Sobol,M., Hozak,P., Kren,L., Katsetos,C.D. and
            Draber,P.
  TITLE     Differential expression of human gamma-tubulin isotypes during
            neuronal development and oxidative stress points to a
            gamma-tubulin-2 prosurvival function
  JOURNAL   FASEB J 31 (5), 1828-1846 (2017)
   PUBMED   28119396
  REMARK    GeneRIF: that in the face of predominant gamma-tubulin-1
            expression, the accumulation of gamma-tubulin-2 in mature neurons
            and neuroblastoma cells during oxidative stress may denote a
            prosurvival role of gamma-tubulin-2 in neurons
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Ohashi,T., Yamamoto,T., Yamanashi,Y. and Ohsugi,M.
  TITLE     Human TUBG2 gene is expressed as two splice variant mRNA and
            involved in cell growth
  JOURNAL   FEBS Lett 590 (8), 1053-1063 (2016)
   PUBMED   27015882
  REMARK    GeneRIF: expression of gamma-tubulin2 along with gamma-tubulin1 and
            a novel TUBG2 splice variant are identified.
REFERENCE   3  (bases 1 to 1782)
  AUTHORS   Maounis,N.F., Draberova,E., Mahera,E., Chorti,M., Caracciolo,V.,
            Sulimenko,T., Riga,D., Trakas,N., Emmanouilidou,A., Giordano,A.,
            Draber,P. and Katsetos,C.D.
  TITLE     Overexpression of gamma-tubulin in non-small cell lung cancer
  JOURNAL   Histol Histopathol 27 (9), 1183-1194 (2012)
   PUBMED   22806905
  REMARK    GeneRIF: Our results reveal for the first time an increased
            expression of TUBG1 and TUBG2 in lung cancer
REFERENCE   4  (bases 1 to 1782)
  AUTHORS   Vinopal,S., Cernohorska,M., Sulimenko,V., Sulimenko,T., Vosecka,V.,
            Flemr,M., Draberova,E. and Draber,P.
  TITLE     gamma-Tubulin 2 nucleates microtubules and is downregulated in
            mouse early embryogenesis
  JOURNAL   PLoS One 7 (1), e29919 (2012)
   PUBMED   22235350
  REMARK    GeneRIF: gamma-tubulin 2 is able to nucleate microtubules and
            substitute for gamma-tubulin 1
            Erratum:[PLoS One. 2012;7(6):
            doi/10.1371/annotation/5dd084b1-20e6-4e1f-88e0-dfe05289da08]
REFERENCE   5  (bases 1 to 1782)
  AUTHORS   Bharti,P., Schliebs,W., Schievelbusch,T., Neuhaus,A., David,C.,
            Kock,K., Herrmann,C., Meyer,H.E., Wiese,S., Warscheid,B., Theiss,C.
            and Erdmann,R.
  TITLE     PEX14 is required for microtubule-based peroxisome motility in
            human cells
  JOURNAL   J Cell Sci 124 (Pt 10), 1759-1768 (2011)
   PUBMED   21525035
REFERENCE   6  (bases 1 to 1782)
  AUTHORS   Niwa,J., Ishigaki,S., Doyu,M., Suzuki,T., Tanaka,K. and Sobue,G.
  TITLE     A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin
            ligase activity
  JOURNAL   Biochem Biophys Res Commun 281 (3), 706-713 (2001)
   PUBMED   11237715
REFERENCE   7  (bases 1 to 1782)
  AUTHORS   Herreros,L., Rodriguez-Fernandez,J.L., Brown,M.C.,
            Alonso-Lebrero,J.L., Cabanas,C., Sanchez-Madrid,F., Longo,N.,
            Turner,C.E. and Sanchez-Mateos,P.
  TITLE     Paxillin localizes to the lymphocyte microtubule organizing center
            and associates with the microtubule cytoskeleton
  JOURNAL   J Biol Chem 275 (34), 26436-26440 (2000)
   PUBMED   10840040
REFERENCE   8  (bases 1 to 1782)
  AUTHORS   Wise,D.O., Krahe,R. and Oakley,B.R.
  TITLE     The gamma-tubulin gene family in humans
  JOURNAL   Genomics 67 (2), 164-170 (2000)
   PUBMED   10903841
REFERENCE   9  (bases 1 to 1782)
  AUTHORS   Tassin,A.M., Celati,C., Moudjou,M. and Bornens,M.
  TITLE     Characterization of the human homologue of the yeast spc98p and its
            association with gamma-tubulin
  JOURNAL   J Cell Biol 141 (3), 689-701 (1998)
   PUBMED   9566969
REFERENCE   10 (bases 1 to 1782)
  AUTHORS   Hsu,H.L. and Yeh,N.H.
  TITLE     Dynamic changes of NuMA during the cell cycle and possible
            appearance of a truncated form of NuMA during apoptosis
  JOURNAL   J Cell Sci 109 (Pt 2), 277-288 (1996)
   PUBMED   8838651
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC353745.1 and BC108739.1.
            
            On Nov 22, 2018 this sequence version replaced NM_016437.2.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region, compared to variant 1. It encodes
            isoform 2 which has a shorter N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.367929.1,
                                           SRR3476690.1097203.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000251412.8/ ENSP00000251412.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DC353745.1         37-39
            4-1782              BC108739.1         1-1779
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.2"
     gene            1..1782
                     /gene="TUBG2"
                     /note="tubulin gamma 2"
                     /db_xref="GeneID:27175"
                     /db_xref="HGNC:HGNC:12419"
                     /db_xref="MIM:605785"
     exon            1..269
                     /gene="TUBG2"
                     /inference="alignment:Splign:2.1.0"
     CDS             221..1576
                     /gene="TUBG2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     gamma-2-tubulin; tubulin gamma 2 chain"
                     /codon_start=1
                     /product="tubulin gamma-2 chain isoform 2"
                     /protein_id="NP_057521.1"
                     /db_xref="CCDS:CCDS32658.1"
                     /db_xref="GeneID:27175"
                     /db_xref="HGNC:HGNC:12419"
                     /db_xref="MIM:605785"
                     /translation="MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEG
                     TDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGN
                     NWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDR
                     YPKKLVQTYSVFPYQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRL
                     HIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPL
                     TTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVH
                     KSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFESSC
                     QQFDKLRKRDAFLEQFRKEDMFKDNFDEMDRSREVVQELIDEYHAATQPDYISWGTQE
                     Q"
     exon            270..382
                     /gene="TUBG2"
                     /inference="alignment:Splign:2.1.0"
     exon            383..550
                     /gene="TUBG2"
                     /inference="alignment:Splign:2.1.0"
     exon            551..619
                     /gene="TUBG2"
                     /inference="alignment:Splign:2.1.0"
     exon            620..699
                     /gene="TUBG2"
                     /inference="alignment:Splign:2.1.0"
     exon            700..826
                     /gene="TUBG2"
                     /inference="alignment:Splign:2.1.0"
     exon            827..913
                     /gene="TUBG2"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1063
                     /gene="TUBG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..1216
                     /gene="TUBG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1378
                     /gene="TUBG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1782
                     /gene="TUBG2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1752..1757
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUBG2"
                     /note="hexamer: AATAAA"
     polyA_site      1782
                     /gene="TUBG2"
                     /note="major polyA site"
ORIGIN      
        1 attccagctg cgactgctga gggagaaaat gatgcccagg ttgggctccc cggcccaccg
       61 gccgaggaga ggcctgcgct gcacacgcgc agaccgagca tccgcgtcaa gaggcgaaga
      121 gagcgcgcgc tccccacgtc ctgcgctcct ggctgccggg cattcgtctc agccgtgact
      181 ctcgccaggc cggggctggc gcgcccacgt ctgaagagcg atgccccggg agatcatcac
      241 cctgcagctg ggccagtgcg gcaaccagat tgggttcgag ttctggaaac agctgtgcgc
      301 cgagcatggt atcagccccg agggcatcgt ggaggaattc gccaccgagg gcactgaccg
      361 caaggacgtc tttttctacc aggcagacga tgagcactac atcccccggg ccgtgctgct
      421 ggacttggaa ccccgggtga tccactccat cctcaactcc ccctatgcca agctctacaa
      481 cccagagaac atctacctgt cggaacatgg aggaggagct ggcaacaact gggccagcgg
      541 attctcccag ggtgagaaaa ttcatgaaga catctttgac atcatagacc gagaagcaga
      601 tggaagtgac agtttggagg gcttcgtgct gtgtcactcc atcgctgggg gtacgggttc
      661 tggcctgggc tcctacctcc tggagcgact gaatgacagg taccccaaga agctagtgca
      721 gacttattca gtgtttccct accaggacga gatgagcgac gtagtggttc agccctacaa
      781 ttcactcctg acactcaaga ggctgacgca gaacgcagat tgtgtggtgg tgctggacaa
      841 cacagccctg aaccggattg ccacagaccg cctgcacatc cagaacccgt ccttctccca
      901 gatcaaccag ctggtgtcca ccatcatgtc ggccagcacc accaccctgc gctaccccgg
      961 ctacatgaac aatgacctca tcggcctcat cgcctcgctc attcccaccc cacggctcca
     1021 cttcctcatg accggctaca ccccgctcac tacagaccag tcagtggcca gcgtgaggaa
     1081 gaccacggtc ctggatgtca tgaggcggct gctgcagccc aagaacgtga tggtgtccac
     1141 aggccgagac cgccagacca accactgcta catcgccatc ctcaacatca tccagggaga
     1201 ggtggacccc acccaggtcc acaagagcct gcagaggatc cgggaacgga agttggccaa
     1261 cttcatcccg tggggccccg ccagcatcca ggtggccctg tcgaggaagt ctccctacct
     1321 gccctcggcc caccgggtca gcgggctcat gatggccaac cacaccagca tctcctcgct
     1381 ctttgaaagt tcctgccagc agtttgacaa gctgcggaag cgggatgcct tcctcgagca
     1441 gttccgtaag gaggacatgt tcaaggacaa ctttgatgag atggacaggt ctagggaggt
     1501 tgttcaggag ctcattgatg agtaccatgc ggccacccag ccagactaca tttcctgggg
     1561 cacccaggag cagtgatttc cctccccact actccttctc cttctagatg gtaaccacag
     1621 cctcgaccat gcctgctccc tctgacccag cttcacctca tggacaaccc ttcttggttc
     1681 atctccagcc cgtgagctgg tcctgcttcc tcccttccat gccctaactt ttaatatgct
     1741 tgttcagctc taataaagta ataaagcttg gttgtcagta ta
//
