LOCUS       NM_032525               1879 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens tubulin beta 6 class V (TUBB6), transcript variant 1,
            mRNA.
ACCESSION   NM_032525 XM_940079
VERSION     NM_032525.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Kim,B., Jung,M., Moon,K.C., Han,D., Kim,K., Kim,H., Yang,S.,
            Lee,D., Jun,H., Lee,K.M., Lee,C.H., Nikas,I.P., Yang,S., Lee,H. and
            Ryu,H.S.
  TITLE     Quantitative proteomics identifies TUBB6 as a biomarker of
            muscle-invasion and poor prognosis in bladder cancer
  JOURNAL   Int J Cancer 152 (2), 320-330 (2023)
   PUBMED   36054443
  REMARK    GeneRIF: Quantitative proteomics identifies TUBB6 as a biomarker of
            muscle-invasion and poor prognosis in bladder cancer.
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 1879)
  AUTHORS   Guerit,D., Marie,P., Morel,A., Maurin,J., Verollet,C.,
            Raynaud-Messina,B., Urbach,S. and Blangy,A.
  TITLE     Primary myeloid cell proteomics and transcriptomics: importance of
            beta-tubulin isotypes for osteoclast function
  JOURNAL   J Cell Sci 133 (10) (2020)
   PUBMED   32265273
  REMARK    GeneRIF: Primary myeloid cell proteomics and transcriptomics:
            importance of beta-tubulin isotypes for osteoclast function.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1879)
  AUTHORS   Lee,J.H., Han,J.H., Kim,H., Park,S.M., Joe,E.H. and Jou,I.
  TITLE     Parkinson's disease-associated LRRK2-G2019S mutant acts through
            regulation of SERCA activity to control ER stress in astrocytes
  JOURNAL   Acta Neuropathol Commun 7 (1), 68 (2019)
   PUBMED   31046837
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1879)
  AUTHORS   Beggs,A.D., James,J., Caldwell,G., Prout,T., Dilworth,M.P.,
            Taniere,P., Iqbal,T., Morton,D.G. and Matthews,G.
  TITLE     Discovery and Validation of Methylation Biomarkers for Ulcerative
            Colitis Associated Neoplasia
  JOURNAL   Inflamm Bowel Dis 24 (7), 1503-1509 (2018)
   PUBMED   29762666
  REMARK    GeneRIF: Methylation in the beta-tubulin TUBB6 correlated with the
            presence of dysplasia and accurately discriminated between
            dysplasia and nondysplastic tissue, even in the apparently normal
            field mucosa downstream from dysplastic lesions. Methylation in
            TUBB6 is a potential biomarker for Ulcerative colitis (UC) -
            associated dysplasia.
REFERENCE   6  (bases 1 to 1879)
  AUTHORS   Leandro-Garcia,L.J., Leskela,S., Landa,I., Montero-Conde,C.,
            Lopez-Jimenez,E., Leton,R., Cascon,A., Robledo,M. and
            Rodriguez-Antona,C.
  TITLE     Tumoral and tissue-specific expression of the major human
            beta-tubulin isotypes
  JOURNAL   Cytoskeleton (Hoboken) 67 (4), 214-223 (2010)
   PUBMED   20191564
  REMARK    GeneRIF: The TUBB6 expression was largely decreased in most tumors.
REFERENCE   7  (bases 1 to 1879)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   8  (bases 1 to 1879)
  AUTHORS   Cucchiarelli,V., Hiser,L., Smith,H., Frankfurter,A., Spano,A.,
            Correia,J.J. and Lobert,S.
  TITLE     Beta-tubulin isotype classes II and V expression patterns in
            nonsmall cell lung carcinomas
  JOURNAL   Cell Motil Cytoskeleton 65 (8), 675-685 (2008)
   PUBMED   18613117
REFERENCE   9  (bases 1 to 1879)
  AUTHORS   Verdier-Pinard,P., Shahabi,S., Wang,F., Burd,B., Xiao,H.,
            Goldberg,G.L., Orr,G.A. and Horwitz,S.B.
  TITLE     Detection of human betaV-tubulin expression in epithelial cancer
            cell lines by tubulin proteomics
  JOURNAL   Biochemistry 44 (48), 15858-15870 (2005)
   PUBMED   16313188
REFERENCE   10 (bases 1 to 1879)
  AUTHORS   Gigot,D., Caplier,I., Strosberg,D., Pierard,A. and Glansdorff,N.
  TITLE     Amino-proximal sequences of the argF and argI ornithine
            carbamoyltransferases from Escherichia coli K-12 [proceedings]
  JOURNAL   Arch Int Physiol Biochim 86 (4), 913-915 (1978)
   PUBMED   84617
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK001295.1 and CD364877.1.
            
            On May 17, 2019 this sequence version replaced NM_032525.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). Both variants 1 and 2 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK001295.1, AK022340.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000317702.10/ ENSP00000318697.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1831              AK001295.1         1-1831
            1832-1879           CD364877.1         18-65               c
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.21"
     gene            1..1879
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
                     /note="tubulin beta 6 class V"
                     /db_xref="GeneID:84617"
                     /db_xref="HGNC:HGNC:20776"
                     /db_xref="MIM:615103"
     exon            1..109
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..1393
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
                     /note="isoform 1 is encoded by transcript variant 1; class
                     V beta-tubulin; tubulin beta MGC4083; tubulin beta-6
                     chain; tubulin beta class V; tubulin, beta 6"
                     /codon_start=1
                     /product="tubulin beta-6 chain isoform 1"
                     /protein_id="NP_115914.1"
                     /db_xref="CCDS:CCDS11858.1"
                     /db_xref="GeneID:84617"
                     /db_xref="HGNC:HGNC:20776"
                     /db_xref="MIM:615103"
                     /translation="MREIVHIQAGQCGNQIGTKFWEVISDEHGIDPAGGYVGDSALQL
                     ERINVYYNESSSQKYVPRAALVDLEPGTMDSVRSGPFGQLFRPDNFIFGQTGAGNNWA
                     KGHYTEGAELVDAVLDVVRKECEHCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFP
                     DRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLT
                     TPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSR
                     GSQQYRALTVPELTQQMFDARNMMAACDPRHGRYLTVATVFRGPMSMKEVDEQMLAIQ
                     SKNSSYFVEWIPNNVKVAVCDIPPRGLKMASTFIGNSTAIQELFKRISEQFSAMFRRK
                     AFLHWFTGEGMDEMEFTEAESNMNDLVSEYQQYQDATANDGEEAFEDEEEEIDG"
     exon            110..218
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
                     /inference="alignment:Splign:2.1.0"
     exon            219..329
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
                     /inference="alignment:Splign:2.1.0"
     exon            330..1879
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1807..1812
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
                     /note="hexamer: AATAAA"
     regulatory      1815..1820
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
                     /note="hexamer: ACTAAA"
     polyA_site      1832
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
                     /note="major polyA site"
     regulatory      1849..1854
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
                     /note="hexamer: CATAAA"
     polyA_site      1854
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
     polyA_site      1879
                     /gene="TUBB6"
                     /gene_synonym="FPVEPD; HsT1601; TUBB-5"
ORIGIN      
        1 agttgccgct gtcgtccgca gagccagttc ctagcgcaga gccgcgcccg ccatgaggga
       61 gatcgtgcac atccaggcgg gccagtgcgg gaaccagatc ggcaccaagt tttgggaagt
      121 gatcagcgat gagcacggca tcgacccggc cggaggctac gtgggagact cggcgctgca
      181 gctggagaga atcaacgtct actacaatga gtcatcgtct cagaaatatg tgcccagggc
      241 cgccctggtg gacttagagc caggcaccat ggacagcgtg cggtctgggc cttttgggca
      301 gcttttccgg cctgacaact tcatctttgg ccagacgggt gcagggaaca actgggcgaa
      361 agggcactac acggagggcg cggagctggt ggacgcagtg ctggacgtgg tgcggaagga
      421 gtgcgagcac tgcgactgcc tgcagggctt ccagctcacg cactcgctgg gcggcggcac
      481 gggctcaggc atgggcacgc tgctcatcag caagatccgt gaggagttcc cggaccgcat
      541 catgaacacc ttcagcgtca tgccctcgcc caaggtgtcg gacacggtgg tggagcccta
      601 caatgccaca ctgtcggtgc accagctggt ggagaataca gacgagacct actgcatcga
      661 caacgaggcg ctctatgaca tctgcttccg cactctgaag ctgacaacgc ccacctacgg
      721 ggacctcaac cacctggtgt ccgccaccat gagtggggtc accacctcgc tgcgcttccc
      781 gggccagctc aatgctgacc tgcgcaagct ggcggtgaac atggtgccct tcccgcgcct
      841 gcacttcttc atgcctggct tcgcgccgct caccagccgc ggcagccagc agtaccgggc
      901 cctgaccgtg cccgagctca cccagcagat gttcgacgcc aggaacatga tggccgcctg
      961 cgatccgcgc catggccgct acctgaccgt ggccaccgtg ttccgcgggc ccatgtccat
     1021 gaaggaggtg gacgagcaga tgctggccat ccagagtaag aacagcagct acttcgtgga
     1081 gtggattccc aacaacgtga aggtggccgt gtgcgacatc ccgccccgcg gcctgaagat
     1141 ggcctccacc ttcatcggca acagcacggc catccaggag ctgttcaagc gcatctccga
     1201 gcagttctca gccatgttcc ggcgcaaggc cttcctgcac tggttcacgg gtgagggcat
     1261 ggatgaaatg gagttcaccg aggcggagag caacatgaac gacctggtat ccgagtacca
     1321 gcagtaccag gatgccaccg ccaatgacgg ggaggaagct tttgaggatg aggaagagga
     1381 gatcgatgga tagtcggaat agagccgccc caactcagat cctacaacac gcaagttcct
     1441 tcttgaaccc tggtgcctcc taccctatgg ccctgaatgg tgcactggtt taattgtgtt
     1501 ggtgtcggcc cctcacaaat gcagccaagt catgtaatta gtcatctgga acaaagacta
     1561 aaaacagcag agaattgcgg gttctaccca gtcagaagat cacaccatgg agactttcta
     1621 ctagaggact tgaaagagaa ctgaggggcc acaaaataaa cttcaccttc cattaagtgt
     1681 tcaagcatgt ctgcaaatta ggagggagtt agaaacagtc tttttcatcc tttgtgatga
     1741 agcctgaaat tgtgccgtgt tgccttatat gaatatgcag tatgggactt tgaaataatg
     1801 attcataata aaatactaaa cgtgtgtctt catctctcta gccatgtgca taaatgacgt
     1861 aagttacttt gtatggtta
//
