LOCUS       NM_032646               3433 bp    mRNA    linear   PRI 16-JUN-2024
DEFINITION  Homo sapiens tweety family member 2 (TTYH2), transcript variant 1,
            mRNA.
ACCESSION   NM_032646
VERSION     NM_032646.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Sukalskaia,A., Straub,M.S., Deneka,D., Sawicka,M. and Dutzler,R.
  TITLE     Cryo-EM structures of the TTYH family reveal a novel architecture
            for lipid interactions
  JOURNAL   Nat Commun 12 (1), 4893 (2021)
   PUBMED   34385445
  REMARK    GeneRIF: Cryo-EM structures of the TTYH family reveal a novel
            architecture for lipid interactions.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Lu,Q., Liu,J., Zhao,S., Gomez Castro,M.F., Laurent-Rolle,M.,
            Dong,J., Ran,X., Damani-Yokota,P., Tang,H., Karakousi,T., Son,J.,
            Kaczmarek,M.E., Zhang,Z., Yeung,S.T., McCune,B.T., Chen,R.E.,
            Tang,F., Ren,X., Chen,X., Hsu,J.C.C., Teplova,M., Huang,B.,
            Deng,H., Long,Z., Mudianto,T., Jin,S., Lin,P., Du,J., Zang,R.,
            Su,T.T., Herrera,A., Zhou,M., Yan,R., Cui,J., Zhu,J., Zhou,Q.,
            Wang,T., Ma,J., Koralov,S.B., Zhang,Z., Aifantis,I., Segal,L.N.,
            Diamond,M.S., Khanna,K.M., Stapleford,K.A., Cresswell,P., Liu,Y.,
            Ding,S., Xie,Q. and Wang,J.
  TITLE     SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells
            through C-type lectin receptors and Tweety family member 2
  JOURNAL   Immunity 54 (6), 1304-1319 (2021)
   PUBMED   34048708
  REMARK    GeneRIF: SARS-CoV-2 exacerbates proinflammatory responses in
            myeloid cells through C-type lectin receptors and Tweety family
            member 2.
REFERENCE   3  (bases 1 to 3433)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3433)
  AUTHORS   Moon,D.K., Bae,Y.J., Jeong,G.R., Cho,C.H. and Hwang,S.C.
  TITLE     Upregulated TTYH2 expression is critical for the invasion and
            migration of U2OS human osteosarcoma cell lines
  JOURNAL   Biochem Biophys Res Commun 516 (2), 521-525 (2019)
   PUBMED   31230749
  REMARK    GeneRIF: TTYH2 may serve as a novel target for the treatment of
            osteosarcoma.
REFERENCE   5  (bases 1 to 3433)
  AUTHORS   Ryu,J., Kim,D.G., Lee,Y.S., Bae,Y., Kim,A., Park,N., Hwang,E.M. and
            Park,J.Y.
  TITLE     Surface expression of TTYH2 is attenuated by direct interaction
            with beta-COP
  JOURNAL   BMB Rep 52 (7), 445-450 (2019)
   PUBMED   30670146
  REMARK    GeneRIF: data suggested that beta-COP plays a critical role in the
            trafficking of the TTYH2 channel to the plasma membrane
REFERENCE   6  (bases 1 to 3433)
  AUTHORS   He,Y., Ramsay,A.J., Hunt,M.L., Whitbread,A.K., Myers,S.A. and
            Hooper,J.D.
  TITLE     N-glycosylation analysis of the human Tweety family of putative
            chloride ion channels supports a penta-spanning membrane
            arrangement: impact of N-glycosylation on cellular processing of
            Tweety homologue 2 (TTYH2)
  JOURNAL   Biochem J 412 (1), 45-55 (2008)
   PUBMED   18260827
  REMARK    GeneRIF: N-glycosylation is not the determining factor for TTYH2
            trafficking to the plasma membrane.
REFERENCE   7  (bases 1 to 3433)
  AUTHORS   Toiyama,Y., Mizoguchi,A., Kimura,K., Hiro,J., Inoue,Y., Tutumi,T.,
            Miki,C. and Kusunoki,M.
  TITLE     TTYH2, a human homologue of the Drosophila melanogaster gene
            tweety, is up-regulated in colon carcinoma and involved in cell
            proliferation and cell aggregation
  JOURNAL   World J Gastroenterol 13 (19), 2717-2721 (2007)
   PUBMED   17569141
  REMARK    GeneRIF: TTYH2 gene expression is significantly up-regulated in
            colon cancer. The TTYH2 gene may play an important role in
            regulating both proliferating and metastatic potentials of
            colorectal cancer.
REFERENCE   8  (bases 1 to 3433)
  AUTHORS   Suzuki,M. and Mizuno,A.
  TITLE     A novel human Cl(-) channel family related to Drosophila flightless
            locus
  JOURNAL   J Biol Chem 279 (21), 22461-22468 (2004)
   PUBMED   15010458
REFERENCE   9  (bases 1 to 3433)
  AUTHORS   Ibarrola,N., Molina,H., Iwahori,A. and Pandey,A.
  TITLE     A novel proteomic approach for specific identification of tyrosine
            kinase substrates using [13C]tyrosine
  JOURNAL   J Biol Chem 279 (16), 15805-15813 (2004)
   PUBMED   14739304
REFERENCE   10 (bases 1 to 3433)
  AUTHORS   Rae,F.K., Hooper,J.D., Eyre,H.J., Sutherland,G.R., Nicol,D.L. and
            Clements,J.A.
  TITLE     TTYH2, a human homologue of the Drosophila melanogaster gene
            tweety, is located on 17q24 and upregulated in renal cell carcinoma
  JOURNAL   Genomics 77 (3), 200-207 (2001)
   PUBMED   11597145
  REMARK    GeneRIF: expressed most highly in brain and testis and at lower
            levels in heart, ovary, spleen, and peripheral blood leukocytes.
            Expression of this gene is upregulated in 13 of 16 (81%) renal cell
            carcinoma samples examined
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BF529231.1, BC005168.1,
            AF319952.1, AL831858.1, AK058197.1, AK126955.1 and BC004233.2.
            
            On Nov 22, 2018 this sequence version replaced NM_032646.5.
            
            Summary: This gene encodes a member of the tweety family of
            proteins. Members of this family function as chloride anion
            channels. The encoded protein functions as a calcium(2+)-activated
            large conductance chloride(-) channel, and may play a role in
            kidney tumorigenesis. Two transcript variants encoding distinct
            isoforms have been identified for this gene. [provided by RefSeq,
            Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript, and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.10951.1,
                                           SRR1660805.141609.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000269346.9/ ENSP00000269346.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                BF529231.1         17-27
            12-800              BC005168.1         1-789
            801-1739            AF319952.1         794-1732
            1740-2526           AL831858.1         500-1286
            2527-2866           AK058197.1         1241-1580
            2867-3052           AK126955.1         3099-3284
            3053-3205           AL831858.1         1813-1965
            3206-3429           AF319952.1         3197-3420
            3430-3433           BC004233.2         1522-1525
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.1"
     gene            1..3433
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /note="tweety family member 2"
                     /db_xref="GeneID:94015"
                     /db_xref="HGNC:HGNC:13877"
                     /db_xref="MIM:608855"
     exon            1..146
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     CDS             18..1622
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein tweety homolog 2; hTTY2; volume-regulated anion
                     channel subunit TTYH2"
                     /codon_start=1
                     /product="protein tweety homolog 2 isoform 1"
                     /protein_id="NP_116035.5"
                     /db_xref="CCDS:CCDS32717.1"
                     /db_xref="GeneID:94015"
                     /db_xref="HGNC:HGNC:13877"
                     /db_xref="MIM:608855"
                     /translation="MQAARVDYIAPWWVVWLHSVPHVGLRLQPVNSTFSPGDESYQES
                     LLFLGLVAAVCLGLNLIFLVAYLVCACHCRRDDAVQTKQHHSCCITWTAVVAGLICCA
                     AVGVGFYGNSETNDGAYQLMYSLDDANHTFSGIDALVSGTTQKMKVDLEQHLARLSEI
                     FAARGDYLQTLKFIQQMAGSVVVQLSGLPVWREVTMELTKLSDQTGYVEYYRWLSYLL
                     LFILDLVICLIACLGLAKRSKCLLASMLCCGALSLLLSWASLAADGSAAVATSDFCVA
                     PDTFILNVTEGQISTEVTRYYLYCSQSGSSPFQQTLTTFQRALTTMQIQVAGLLQFAV
                     PLFSTAEEDLLAIQLLLNSSESSLHQLTAMVDCRGLHKDYLDALAGICYDGLQGLLYL
                     GLFSFLAALAFSTMICAGPRAWKHFTTRNRDYDDIDDDDPFNPQAWRMAAHSPPRGQL
                     HSFCSYSSGLGSQTSLQPPAQTISNAPVSEYMNQAMLFGRNPRYENVPLIGRASPPPT
                     YSPSMRATYLSVADEHLRHYGNQFPA"
     exon            147..319
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            320..431
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            432..652
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            653..748
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            749..821
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            822..891
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            892..947
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1040
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1133
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1276
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1462
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1541
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..3433
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3413..3418
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /note="hexamer: AATAAA"
     polyA_site      3433
                     /gene="TTYH2"
                     /gene_synonym="C17orf29"
                     /note="major polyA site"
ORIGIN      
        1 agcactcggg ccgagccatg caggcggcgc gcgtggacta catcgctccc tggtgggtcg
       61 tgtggctgca cagcgtcccg cacgtcggcc tgcgcctgca gcccgtgaac agcaccttca
      121 gccccggcga cgagagttac caggagtcgc tgctgttcct ggggctggtg gccgccgtct
      181 gcctgggcct gaacctcatc ttccttgtgg cttacctggt ctgtgcatgc cactgccggc
      241 gggacgatgc ggtgcagacc aagcagcacc actcctgctg catcacctgg acggccgtgg
      301 tggccgggct catctgctgt gctgcggtgg gcgttggttt ctatggaaac agcgagacca
      361 acgatggggc gtaccagctg atgtactcct tggacgatgc caaccacacc ttctctggga
      421 tcgatgctct ggtttccgga actacccaga agatgaaggt ggacctagag cagcacctgg
      481 cccggctcag tgagatcttt gctgcccggg gcgattacct gcagaccctg aagttcatac
      541 agcagatggc gggcagcgtt gttgttcagc tctcaggact gcccgtgtgg agggaggtca
      601 ccatggagct gaccaagcta tccgaccaga ctggctacgt ggagtactac aggtggctct
      661 cctacctcct gctctttatc ctggacctgg tcatctgcct cattgcctgc ctgggactgg
      721 ccaagcgctc caagtgtctc ctggcctcga tgctgtgctg tggggcactg agcctgctcc
      781 tcagttgggc atccctggcc gctgatggct ctgcggcagt ggccaccagt gacttctgtg
      841 tggctcctga caccttcatc ctgaacgtca cggagggcca gatcagcaca gaggtgactc
      901 gctactacct gtattgcagc cagagtggaa gcagcccctt ccagcagacc ctgaccacct
      961 tccagcgcgc acttaccacc atgcagatcc aggtcgcggg gctgctgcag tttgccgtgc
     1021 ccctcttctc cactgcagag gaagacctgc ttgcaatcca gctcctgctg aactcctcag
     1081 agtccagcct tcaccagctg accgccatgg tggactgccg agggctgcac aaggattatc
     1141 tggacgctct tgctggcatc tgctacgacg gcctccaggg cttgctgtac cttggcctct
     1201 tctccttcct ggccgccctc gccttctcca ccatgatctg tgcagggcca agggcctgga
     1261 agcacttcac caccagaaac agagactacg atgacattga tgatgatgac ccctttaacc
     1321 cccaagcctg gcgcatggcg gctcacagtc ccccgagggg acagcttcac agcttctgca
     1381 gctacagcag tggcctggga agtcagacca gcctgcagcc cccggcccag accatctcca
     1441 acgcccctgt ctccgagtac atgaaccaag ccatgctctt tggtaggaac ccacgctacg
     1501 agaacgtgcc actaatcggg agagcctccc ctccgcctac gtactctccc agcatgagag
     1561 ccacctacct gtctgtggcg gatgagcacc tgaggcacta cgggaatcag tttccagcct
     1621 aacagacttt cgggggttcc tgcctccttt ttccgttctg gtttttaatt agtgcaaata
     1681 caagctgcgt ttctttaata gaaaccaaag gcatctggag cccgagaggc ctcctgctgt
     1741 ggcagaggag cagctgggat tcccgaccaa agccccaggg ggtgcagaag actcaccacg
     1801 cgggccagcc tctctctttt gccctgctct ccacaccaga aatgccccca ggtgcttggc
     1861 tgcctcagag gtaccatccc tgagctggct gcctggccct gctcacccct acgcctcgcc
     1921 cttgccagga ggggagtggc agtgaggagg gggccaggtc aggcaccacc atcaagagag
     1981 ctgtgtgttc tctctggtcc cacaacgatg actctgcctc ttgtcagccc agccaagagc
     2041 ccagacgacc cctctgtcct cgttccctgt cctcgttccc tgcaggtaac atgagaaggg
     2101 ctgatcagga gatgctcttt aagaagttcg cacccctgct gacaccagaa cagcccaaat
     2161 cagagttccc agggccagac aggctcttcc tgggccacag aggggaggca tcaggaaagc
     2221 tctgcagtgg ggggctggtg gctccggggc tgggggatca caggctggtg aaccccggtg
     2281 ggaacagagg tgaaagcctg ccacattccg cctgtctccc taaccctcca ttgcctcgcc
     2341 tctattccag aatcaatgct gcagaatgtg ttagctgcag ataggcatgg tctcaggtat
     2401 gaacagacac tttgaaacga ctttaggtct ttcttttctc cagtgtttta aacatgttga
     2461 ttatccaaag aattgaaact cctagcacat ccagttttta caacagattt gcagctcatt
     2521 ccttaccctg gttaggtcac tacttttgca gattttgctg gcactgatct ggagatctgc
     2581 agatctggag gagacgggaa ggagtcgatt cttaaataag gatcagtgag gcatcctgtc
     2641 ccaagctact gtttggtggg gatctgggtt catctcaccc acagagggag gatctttaag
     2701 aggagaaaaa agccaagagg gaaagccaga gttccctgtt ctaggggact agccaaatgc
     2761 ctacatcagc tgtcccctcc ctgttgtctc caagtaagtt tgccagaaaa ggttttagca
     2821 aagtgctaca actgtgtctt tataggagga taggcctctg ccctgcccca cccccaccac
     2881 ctgtccccac ccagtgtccc aggccacagg agcttattgg ccaggaggga ataatgtccc
     2941 ccaatactgc ctgttgaggg accagagttg gggtctttgg tgcttccaac ctcctgccaa
     3001 cctggagttc acaacaccag agccccacgg cctcgcacac tgaagcaggg gcgtgcggtg
     3061 actcggtgct tctgttttgg aagaaccacc tgtcatcaaa acatggacag cagggtgttc
     3121 tcagctccca gcgaagcctc cacaacagaa tggggccaca gggcagccgg gactccctgt
     3181 ctcacctaca ttaacccatg catactgtat gccataaact cactttggta tatccgcgtc
     3241 acatgcagag aggaactctg cgacgtcaaa gtgttgcttc ttaaagtttc attattggca
     3301 actagagggt tgtttttaat gcatggaaac taaacagatt cctcggggag ttcctgaagg
     3361 aaccaggtgg gcaaaccttt gcttatatac atgcggcctc acctggaaga gaaataaacc
     3421 acttgtacta aaa
//
