LOCUS       NM_133379              18220 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens titin (TTN), transcript variant novex-3, mRNA.
ACCESSION   NM_133379
VERSION     NM_133379.5
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 18220)
  AUTHORS   Tskhovrebova,L. and Trinick,J.
  TITLE     Roles of titin in the structure and elasticity of the sarcomere
  JOURNAL   J Biomed Biotechnol 2010, 612482 (2010)
   PUBMED   20625501
  REMARK    GeneRIF: Recent titin data is reviewed and its implications for
            sarcomere architecture and elasticity, are discussed.
            Review article
REFERENCE   2  (bases 1 to 18220)
  AUTHORS   Labeit,S., Lahmers,S., Burkart,C., Fong,C., McNabb,M., Witt,S.,
            Witt,C., Labeit,D. and Granzier,H.
  TITLE     Expression of distinct classes of titin isoforms in striated and
            smooth muscles by alternative splicing, and their conserved
            interaction with filamins
  JOURNAL   J Mol Biol 362 (4), 664-681 (2006)
   PUBMED   16949617
  REMARK    GeneRIF: This suggests that the titin Z2-Zis1 domain can link
            filamins and alpha-actinin together in the periphery of the
            Z-line/dense bodies in a fashion that is conserved in smooth and
            striated muscles.
REFERENCE   3  (bases 1 to 18220)
  AUTHORS   Bang,M.L., Centner,T., Fornoff,F., Geach,A.J., Gotthardt,M.,
            McNabb,M., Witt,C.C., Labeit,D., Gregorio,C.C., Granzier,H. and
            Labeit,S.
  TITLE     The complete gene sequence of titin, expression of an unusual
            approximately 700-kDa titin isoform, and its interaction with
            obscurin identify a novel Z-line to I-band linking system
  JOURNAL   Circ Res 89 (11), 1065-1072 (2001)
   PUBMED   11717165
REFERENCE   4  (bases 1 to 18220)
  AUTHORS   Freiburg,A., Trombitas,K., Hell,W., Cazorla,O., Fougerousse,F.,
            Centner,T., Kolmerer,B., Witt,C., Beckmann,J.S., Gregorio,C.C.,
            Granzier,H. and Labeit,S.
  TITLE     Series of exon-skipping events in the elastic spring region of
            titin as the structural basis for myofibrillar elastic diversity
  JOURNAL   Circ Res 86 (11), 1114-1121 (2000)
   PUBMED   10850961
REFERENCE   5  (bases 1 to 18220)
  AUTHORS   Labeit,S. and Kolmerer,B.
  TITLE     Titins: giant proteins in charge of muscle ultrastructure and
            elasticity
  JOURNAL   Science 270 (5234), 293-296 (1995)
   PUBMED   7569978
REFERENCE   6  (bases 1 to 18220)
  AUTHORS   Pfeffer,G. and Chinnery,P.F.
  TITLE     Hereditary Myopathy with Early Respiratory Failure
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24575448
REFERENCE   7  (bases 1 to 18220)
  AUTHORS   Hackman,P., Savarese,M., Carmignac,V., Udd,B. and Salih,M.A.
  TITLE     Salih Myopathy
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   22238790
REFERENCE   8  (bases 1 to 18220)
  AUTHORS   Cirino,A.L. and Ho,C.
  TITLE     Hypertrophic Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301725
REFERENCE   9  (bases 1 to 18220)
  AUTHORS   Pegoraro,E. and Hoffman,E.P.
  TITLE     Limb-Girdle Muscular Dystrophy Overview - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301582
REFERENCE   10 (bases 1 to 18220)
  AUTHORS   Udd,B. and Hackman,P.
  TITLE     Udd Distal Myopathy - Tibial Muscular Dystrophy
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301498
REFERENCE   11 (bases 1 to 18220)
  AUTHORS   Hershberger,R.E. and Jordan,E.
  TITLE     Dilated Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301486
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC023270.7 and BX537998.1.
            
            On May 31, 2019 this sequence version replaced NM_133379.4.
            
            Summary: This gene encodes a large abundant protein of striated
            muscle. The product of this gene is divided into two regions, a
            N-terminal I-band and a C-terminal A-band. The I-band, which is the
            elastic part of the molecule, contains two regions of tandem
            immunoglobulin domains on either side of a PEVK region that is rich
            in proline, glutamate, valine and lysine. The A-band, which is
            thought to act as a protein-ruler, contains a mixture of
            immunoglobulin and fibronectin repeats, and possesses kinase
            activity. An N-terminal Z-disc region and a C-terminal M-line
            region bind to the Z-line and M-line of the sarcomere,
            respectively, so that a single titin molecule spans half the length
            of a sarcomere. Titin also contains binding sites for muscle
            associated proteins so it serves as an adhesion template for the
            assembly of contractile machinery in muscle cells. It has also been
            identified as a structural protein for chromosomes. Alternative
            splicing of this gene results in multiple transcript variants.
            Considerable variability exists in the I-band, the M-line and the
            Z-disc regions of titin. Variability in the I-band region
            contributes to the differences in elasticity of different titin
            isoforms and, therefore, to the differences in elasticity of
            different muscle types. Mutations in this gene are associated with
            familial hypertrophic cardiomyopathy 9, and autoantibodies to titin
            are produced in patients with the autoimmune disease scleroderma.
            [provided by RefSeq, Feb 2012].
            
            CCDS Note: This CCDS ID represents the TTN novex-3 isoform, which
            has a novel and shorter C-terminus compared to longer isoforms.
            This isoform is supported by data in PMID:11717165, but it should
            be noted that the evidence for the transcript is partial on the 5'
            end, and thus this CCDS representation has an inferred exon
            combination.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: PMID: 11717165
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-212               AC023270.7         65697-65908         c
            213-316             AC023270.7         63037-63140         c
            317-520             AC023270.7         60623-60826         c
            521-808             AC023270.7         58880-59167         c
            809-894             AC023270.7         58310-58395         c
            895-1139            AC023270.7         57972-58216         c
            1140-1470           AC023270.7         53407-53737         c
            1471-1623           AC023270.7         52884-53036         c
            1624-1761           AC023270.7         51889-52026         c
            1762-1887           AC023270.7         50557-50682         c
            1888-2025           AC023270.7         49193-49330         c
            2026-2163           AC023270.7         48463-48600         c
            2164-2301           AC023270.7         47845-47982         c
            2302-2595           AC023270.7         44333-44626         c
            2596-2718           AC023270.7         44105-44227         c
            2719-3000           AC023270.7         42555-42836         c
            3001-3066           AC023270.7         42205-42270         c
            3067-3325           AC023270.7         41291-41549         c
            3326-3389           AC023270.7         41024-41087         c
            3390-3605           AC023270.7         40697-40912         c
            3606-3748           AC023270.7         39606-39748         c
            3749-3954           AC023270.7         38485-38690         c
            3955-4188           AC023270.7         37714-37947         c
            4189-4433           AC023270.7         37359-37603         c
            4434-4705           AC023270.7         36189-36460         c
            4706-4870           AC023270.7         35905-36069         c
            4871-5039           AC023270.7         35634-35802         c
            5040-6733           AC023270.7         33841-35534         c
            6734-7015           AC023270.7         33406-33687         c
            7016-7282           AC023270.7         32692-32958         c
            7283-7555           AC023270.7         32323-32595         c
            7556-7819           AC023270.7         31947-32210         c
            7820-8080           AC023270.7         31594-31854         c
            8081-8341           AC023270.7         29696-29956         c
            8342-8605           AC023270.7         28897-29160         c
            8606-8866           AC023270.7         28545-28805         c
            8867-9127           AC023270.7         28164-28424         c
            9128-9388           AC023270.7         27158-27418         c
            9389-9530           AC023270.7         26499-26640         c
            9531-9696           AC023270.7         26244-26409         c
            9697-9928           AC023270.7         24866-25097         c
            9929-10213          AC023270.7         23012-23296         c
            10214-10339         AC023270.7         22662-22787         c
            10340-10528         AC023270.7         17469-17657         c
            10529-10585         AC023270.7         11609-11665         c
            10586-16531         AC023270.7         4579-10524          c
            16532-18220         BX537998.1         3284-4972
FEATURES             Location/Qualifiers
     source          1..18220
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.2"
     gene            1..18220
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /note="titin"
                     /db_xref="GeneID:7273"
                     /db_xref="HGNC:HGNC:12403"
                     /db_xref="MIM:188840"
     exon            1..212
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            213..316
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     CDS             226..17040
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /EC_number="2.7.11.1"
                     /note="isoform novex-3 is encoded by transcript variant
                     novex-3; connectin; rhabdomyosarcoma antigen MU-RMS-40.14"
                     /codon_start=1
                     /product="titin isoform novex-3"
                     /protein_id="NP_596870.2"
                     /db_xref="CCDS:CCDS33337.1"
                     /db_xref="GeneID:7273"
                     /db_xref="HGNC:HGNC:12403"
                     /db_xref="MIM:188840"
                     /translation="MTTQAPTFTQPLQSVVVLEGSTATFEAHISGFPVPEVSWFRDGQ
                     VISTSTLPGVQISFSDGRAKLTIPAVTKANSGRYSLKATNGSGQATSTAELLVKAETA
                     PPNFVQRLQSMTVRQGSQVRLQVRVTGIPTPVVKFYRDGAEIQSSLDFQISQEGDLYS
                     LLIAEAYPEDSGTYSVNATNSVGRATSTAELLVQGEEEVPAKKTKTIVSTAQISESRQ
                     TRIEKKIEAHFDARSIATVEMVIDGAAGQQLPHKTPPRIPPKPKSRSPTPPSIAAKAQ
                     LARQQSPSPIRHSPSPVRHVRAPTPSPVRSVSPAARISTSPIRSVRSPLLMRKTQAST
                     VATGPEVPPPWKQEGYVASSSEAEMRETTLTTSTQIRTEERWEGRYGVQEQVTISGAA
                     GAAASVSASASYAAEAVATGAKEVKQDADKSAAVATVVAAVDMARVREPVISAVEQTA
                     QRTTTTAVHIQPAQEQVRKEAEKTAVTKVVVAADKAKEQELKSRTKEVITTKQEQMHV
                     THEQIRKETEKTFVPKVVISAAKAKEQETRISEEITKKQKQVTQEAIRQETEITAASM
                     VVVATAKSTKLETVPGAQEETTTQQDQMHLSYEKIMKETRKTVVPKVIVATPKVKEQD
                     LVSRGREGITTKREQVQITQEKMRKEAEKTALSTIAVATAKAKEQETILRTRETMATR
                     QEQIQVTHGKVDVGKKAEAVATVVAAVDQARVREPREPGHLEESYAQQTTLEYGYKER
                     ISAAKVAEPPQRPASEPHVVPKAVKPRVIQAPSETHIKTTDQKGMHISSQIKKTTDLT
                     TERLVHVDKRPRTASPHFTVSKISVPKTEHGYEASIAGSAIATLQKELSATSSAQKIT
                     KSVKAPTVKPSETRVRAEPTPLPQFPFADTPDTYKSEAGVEVKKEVGVSITGTTVREE
                     RFEVLHGREAKVTETARVPAPVEIPVTPPTLVSGLKNVTVIEGESVTLECHISGYPSP
                     TVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYL
                     AVQVSEEFEKETTAVTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEPAAPYFITKP
                     VVQKLVEGGSVVFGCQVGGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECKLVISMT
                     FADDAGEYTIVVRNKHGETSASASLLEEADYELLMKSQQEMLYQTQVTAFVQEPKVGE
                     TAPGFVYSEYEKEYEKEQALIRKKMAKDTVVVRTYVEDQEFHISSFEERLIKEIEYRI
                     IKTTLEELLEEDGEEKMAVDISESEAVESGFDSRIKNYRILEGMGVTFHCKMSGYPLP
                     KIAWYKDGKRIKHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICSGK
                     LYVEPAAPLGAPTYIPTLEPVSRIRSLSPRSVSRSPIRMSPARMSPARMSPARMSPAR
                     MSPGRRLEETDESQLERLYKPVFVLKPVSFKCLEGQTARFDLKVVGRPMPETFWFHDG
                     QQIVNDYTHKVVIKEDGTQSLIIVPATPSDSGEWTVVAQNRAGRSSISVILTVEAVEH
                     QVKPMFVEKLKNVNIKEGSRLEMKVRATGNPNPDIVWLKNSDIIVPHKYPKIRIEGTK
                     GEAALKIDSTVSQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERKLIIPRGTYR
                     AKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRL
                     TPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYG
                     TDHTSATLIVKDEKSLVEESQLPEGRKGLQRIEELERMAHEGALTGVTTDQKEKQKPD
                     IVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDGIHYLDIV
                     DCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRRAPEPRPEFHVHEPGKLQ
                     FEVQKVDRPVDTTETKEVVKLKRAERITHEKVPEESEELRSKFKRRTEEGYYEAITAV
                     ELKSRKKDESYEELLRKTKDELLHWTKELTEEEKKALAEEGKITIPTFKPDKIELSPS
                     MEAPKIFERIQSQTVGQGSDAHFRVRVVGKPDPECEWYKNGVKIERSDRIYWYWPEDN
                     VCELVIRDVTAEDSASIMVKAINIAGETSSHAFLLVQAKQLITFTQELQDVVAKEKDT
                     MATFECETSEPFVKVKWYKDGMEVHEGDKYRMHSDRKVHFLSILTIDTSDAEDYSCVL
                     VEDENVKTTAKLIVEGAVVEFVKELQDIEVPESYSGELECIVSPENIEGKWYHNDVEL
                     KSNGKYTITSRRGRQNLTVKDVTKEDQGEYSFVIDGKKTTCKLKMKPRPIAILQGLSD
                     QKVCEGDIVQLEVKVSLESVEGVWMKDGQEVQPSDRVHIVIDKQSHMLLIEDMTKEDA
                     GNYSFTIPALGLSTSGRVSVYSVDVITPLKDVNVIEGTKAVLECKVSVPDVTSVKWYL
                     NDEQIKPDDRVQAIVKGTKQRLVINRTHASDEGPYKLIVGRVETNCNLSVEKIKIIRG
                     LRDLTCTETQNVVFEVELSHSGIDVLWNFKDKEIKPSSKYKIEAHGKIYKLTVLNMMK
                     DDEGKYTFYAGENMTSGKLTVAGGAISKPLTDQTVAESQEAVFECEVANPDSKGEWLR
                     DGKHLPLTNNIRSESDGHKRRLIIAATKLDDIGEYTYKVATSKTSAKLKVEAVKIKKT
                     LKNLTVTETQDAVFTVELTHPNVKGVQWIKNGVVLESNEKYAISVKGTIYSLRIKNCA
                     IVDESVYGFRLGRLGASARLHVETVKIIKKPKDVTALENATVAFEVSVSHDTVPVKWF
                     HKSVEIKPSDKHRLVSERKVHKLMLQNISPSDAGEYTAVVGQLECKAKLFVETLHITK
                     TMKNIEVPETKTASFECEVSHFNVPSMWLKNGVEIEMSEKFKIVVQGKLHQLIIMNTS
                     TEDSAEYTFVCGNDQVSATLTVTPIMITSMLKDINAEEKDTITFEVTVNYEGISYKWL
                     KNGVEIKSTDKCQMRTKKLTHSLNIRNVHFGDAADYTFVAGKATSTATLYVEARHIEF
                     RKHIKDIKVLEKKRAMFECEVSEPDITVQWMKDDQELQITDRIKIQKEKYVHRLLIPS
                     TRMSDAGKYTVVAGGNVSTAKLFVEGRDVRIRSIKKEVQVIEKQRAVVEFEVNEDDVD
                     AHWYKDGIEINFQVQERHKYVVERRIHRMFISETRQSDAGEYTFVAGRNRSSVTLYVN
                     APEPPQVLQELQPVTVQSGKPARFCAVISGRPQPKISWYKEEQLLSTGFKCKFLHDGQ
                     EYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPEVVSPDQEMPVYPPAIITP
                     LQDTVTSEGQPARFQCRVSGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYP
                     EDEGTYTFVASNAVGQVSSTANLSLEAPESILHERIEQEIEMEMKELFSEGESEHSER
                     DTRDAFSDSEDIDHKSMAAKRYASRISSTSSWPEYFKPSFTQKLTFKYVLEGEPVVFT
                     CRLIACPTPEMTWFHNNRPIPTGLRRIIKAESDLHHHSSSLEIKRVQDRDSGSYRLLA
                     INSEGSAESTASLLVIQKGQDEKYLEFLKRAERTHENVEALVERGEDRIKVDLRFTGS
                     PFNKKQDVEQKGMMRTIHFKTMSSAKKTDYMYDEEYLESKSDIRGWLNVGESFLDKET
                     KVKLQRLREARKTLMEKKKLSLLDTSSEISSRTLRSEASDKDILFSREDMKIRSMSDL
                     AESYKVDHSAESIVQNPHALSNQMDQNIESEELPTSFQTIVDEEIFQTEIRMSQEALV
                     KESLPKDHLYGEILVNENTQARGQLEEIMANTTIGESSTYITNVCEKEEVYETPENVS
                     QAITPHASESFGTLVNVEESEEIASERIKKDDLRELQLSASTRIDEFKTEQKEENMRF
                     FENSFRKRPQRCPPSFLQEIESQEVYEGDSCNFVCHFQGYPQPIVTWYNNDMPIPRNQ
                     NFIIHSLENYSILTLSSVHHQNEGSITCVLFNQYGTVKTTSMLKVKAKQKHDVKAHKV
                     PVFHDYLDEEEELALVFDQAKGAHPSMSQEGQTNLHLLKTNPPVPPSGDTELLSFPVE
                     IQVTAATPIPEQDKESKEVFQTEELEPKAMPQDQVTQSPKHRFVFLSDITNEPPKMLQ
                     EMPKHARCREGDSIILECLISGEPQPVVTWFQNGVLLKQNQKFQFEEVNCSHQLYIKD
                     VNSQDSGKYKCVAENNSGAVESVSDLTVEPVTYRENSQFENIGEIYGKYSRDQQLQDQ
                     GESVRAHFYDYPAGPFTPWTNVKEYSVRDYFQSLETIEQIDQKEQVRCIPSREKIPRF
                     VHGASRTIKISKPIRAEFIQCQAEGKERHVSEKSKLHQAEGTVYPFVDDFSDVTIKKE
                     IRNNFGKLGRSEKENVQECAQSDYLPNIHSERISDSYNTKDSSAIVYEESLGEEIHYP
                     GKKVKHRIIEFEKLHVEKGVLEKRPTRTSIVNPPQKKIDDKAFSLKQRESRSSNLNAN
                     MYQAEKMSPNTESDSSNIAINLKLLSSQTHKEFDAQEREQQEKISLIDKPAISKRAEH
                     ESPITFDLKQFHTQIKHTDVKFQELDSGQPEEAYFKIQHPADTENIVFDLKQMYSHIG
                     DPALEFQGQETREQQEIHYKEKIPSPETLQPDTHNISKSVQNNVFASQEISSSQELSN
                     RTMVEKSSIDENSISLEKEVRHVQEQNLDILKTDLSLKSFSEEIYSESCALLPTSSAD
                     IEETDLSEKSCPLENGGRSSISHLKKAASEEKPLGVGEMEEECTLEPELAAFPKQDGG
                     TQEYTDATLEDHRGDVQEADTLHRQLSLSQCFPLLMTEEQQNPGEQISTNIHASGEEK
                     CYEEVQVQNEASFSTLEGEMIETSFSQNIPKLDEAHTTEAAESETSLTQYLLAAGKRE
                     VPETKDTRDQAKLVQSESITSMEVEEVTFNTVYEYYNQKQESLGRPLSPESDISIGVG
                     STTSEEISELDQFYTPPSSVEYFESPKSPDLYFNPSDITKQSSIHSGGETVERYSTPL
                     GEVAERYSTPSEGEVGERYSTPPGETLERYSTPPGETLERYSTPPGETLERYSTPPGE
                     TLERYSTPPGETLERYSTPPGEALERYSIPTGGPNPTGTFKTYPSKIEREDGTPNEHF
                     YTPTEERGSAYEIWRSDSFGTPNEAIEPKDNEMPPSFIEPLTKRKVYENTTLGFIVEV
                     EGLPVPGVKWYRNKSLLEPDERIKMERVGNVCSLEISNIQKGEGGEYMCHAVNIIGEA
                     KSFANVDIMPQEERVVALPPPVTHQHVMEFDLEHTTSSRTPSPQEIVLEVELSEKDVK
                     EFEKQVKIVTVPEFTPDHKSMIVSLDVLPFNFVDPNMDSREGEDKELKIDLEVFEMPP
                     RFIMPICDFKIPENSDAVFKCSVIGIPTPEVKWYKEYMCIEPDNIKYVISEEKGSHTL
                     KIRNVCLSDSATYRCRAVNCVGEAICRGFLTMGDSEIFAVIAKKSKVTLSSLMEELVL
                     KSNYTDSFFEFQVVEGPPRFIKGISDCYAPIGTAAYFQCLVRGSPRPTVYWYKDGKLV
                     QGRRFTVEESGTGFHNLFITSLVKSDEGEYRCVATNKSGMAESFAALTLT"
     exon            317..520
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            521..808
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            809..894
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1139
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1470
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1623
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1761
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1887
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..2025
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2163
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2301
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2302..2595
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2718
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2719..3000
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            3001..3066
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            3067..3325
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            3326..3389
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            3390..3605
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            3606..3748
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            3749..3954
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            3955..4188
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            4189..4433
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            4434..4705
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            4706..4870
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            4871..5039
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            5040..6733
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            6734..7015
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            7016..7282
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            7283..7555
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            7556..7819
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            7820..8080
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            8081..8341
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            8342..8605
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            8606..8866
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            8867..9127
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            9128..9388
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            9389..9530
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            9531..9696
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            9697..9928
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            9929..10213
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            10214..10339
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            10340..10528
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            10529..10585
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     exon            10586..18220
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      17283..17288
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /note="hexamer: ATTAAA"
     polyA_site      17307
                     /gene="TTN"
                     /gene_synonym="CMD1G; CMH9; CMPD4; CMYO5; CMYP5; EOMFC;
                     HMERF; LGMD2J; LGMDR10; MYLK5; SALMY; TMD"
                     /note="major polyA site"
ORIGIN      
        1 gagcagtcgt gcattcccag cctcgcctcg ggtgtaggga ttgcatagaa aagcaaaact
       61 acacagtctt gactgtgtag ttttgttttt aggattagag gctcaccgat tcatgtcgga
      121 gatggtcaga aaaaccaact ctccatagga cgtcgtttca gaagcaacct tgggcttagt
      181 cccacccttt ttaggcactc ttgagaaatc agagtgccta gaaagatgac aactcaagca
      241 ccgacgttta cgcagccgtt acaaagcgtt gtggtactgg agggtagtac cgcaaccttt
      301 gaggctcaca ttagtggttt tccagttcct gaggtgagct ggtttaggga tggccaggtg
      361 atttccactt ccactctgcc cggcgtgcag atctccttta gcgatggccg cgctaaactg
      421 acgatccccg ccgtgactaa agccaacagt ggacgatatt ccctgaaagc caccaatgga
      481 tctggacaag cgactagtac tgctgagctt ctcgtgaaag ctgagacagc accacccaac
      541 ttcgttcaac gactgcagag catgaccgtg agacaaggaa gccaagtgag actccaagtg
      601 agagtgactg gaatccctac acctgtggtg aagttctacc gggatggagc cgaaatccag
      661 agctcccttg atttccaaat ttcacaagaa ggcgacctct acagcttact gattgcagaa
      721 gcataccctg aggactcagg gacctattca gtaaatgcca ccaatagcgt tggaagagct
      781 acttcgactg ctgaattact ggttcaaggt gaagaagaag tacctgctaa aaagacaaag
      841 acaattgttt cgactgctca gatctcagaa tcaagacaaa cccgaattga aaagaagatt
      901 gaagcccact ttgatgccag atcaattgca acagttgaga tggtcataga tggtgccgct
      961 gggcaacagc tgccacataa aacacctccc aggattcctc cgaagccaaa gtcaagatcc
     1021 ccaacaccac cgtctattgc tgccaaagca cagctggctc ggcagcagtc cccatcgccc
     1081 ataagacact ccccttcccc ggtcagacac gtgcgggcac cgaccccatc tccggtcagg
     1141 tccgtgtctc cagcagcaag aatctccaca tcccccatca ggtctgttag gtctccattg
     1201 ctcatgcgta agactcaggc atccaccgtg gccacaggtc ctgaagtgcc tcccccttgg
     1261 aagcaagagg gctacgtggc ctcctcatct gaggctgaga tgagagagac aacgctgaca
     1321 acctctactc agatcaggac agaagagaga tgggaaggga gatacggtgt ccaggagcaa
     1381 gtgaccatca gtggtgctgc gggtgctgcc gccagtgtgt cggccagtgc tagctacgca
     1441 gcagaggctg ttgccactgg tgctaaagag gtgaaacaag atgctgacaa aagtgcagct
     1501 gttgcgactg ttgttgctgc cgttgatatg gccagagtga gagaaccagt gatcagcgct
     1561 gtagagcaga ctgctcagag gacaaccacg actgctgtgc acatccaacc tgctcaagaa
     1621 caggtaagaa aggaagcgga gaagactgct gtaactaagg tagtagtggc cgccgataaa
     1681 gccaaggaac aagaattaaa atcaagaacc aaagaagtaa ttaccacaaa gcaagagcag
     1741 atgcacgtaa ctcatgagca gataagaaaa gaaactgaaa aaacatttgt accaaaggta
     1801 gtaatttccg cagctaaagc caaagaacaa gaaactagaa tttctgaaga aattactaag
     1861 aaacagaaac aagtaactca agaagcaata agacaggaaa ctgagataac tgctgcatcc
     1921 atggtggtag ttgccactgc aaagtccaca aaactagaaa cagtcccggg agctcaagaa
     1981 gaaactacca cacaacaaga tcaaatgcac ctaagttatg aaaagataat gaaggaaact
     2041 aggaaaacag ttgtacctaa agtcatagtt gccacaccca aagtcaaaga acaagattta
     2101 gtatcaagag gtagagaagg cattactacc aaaagagaac aagtgcaaat aactcaggag
     2161 aagatgagaa aggaagccga gaaaactgcc ttgtctacaa tagcagttgc tactgctaaa
     2221 gccaaagaac aagaaacaat actgagaact agagaaacta tggctactag acaagaacaa
     2281 atccaagtta cccatggaaa ggtggacgtt ggaaaaaagg ctgaagctgt agcaacagtt
     2341 gttgctgcag tagaccaggc ccgagtcaga gagcccagag agcctgggca tcttgaagaa
     2401 tcctatgctc agcagaccac tttggagtac ggatataagg aacgcatttc cgccgcaaag
     2461 gtagctgagc ctccccaacg tccagcctca gaaccccacg ttgtccctaa agcagtcaag
     2521 cctagagtaa tccaggctcc ttctgagact catatcaaaa ctactgatca aaagggaatg
     2581 cacatatcat cacagatcaa gaaaactaca gatctaacaa cggaaagatt agtccatgtg
     2641 gataaacgcc cccgcacagc tagccctcac tttactgttt caaaaatttc tgttcctaag
     2701 acagaacatg gatatgaggc atcaatagcc ggtagtgcta ttgccacatt acaaaaagag
     2761 ttgtcagcca catcttctgc tcagaagatc accaaatcgg tgaaggctcc tactgtgaag
     2821 cccagtgaga ctagagtaag ggcagagccc acacccttgc cacagttccc cttcgctgac
     2881 acaccagata cttacaagag tgaagctggc gttgaggtga aaaaggaagt aggggtgagc
     2941 atcactggca ccaccgtccg tgaagagcgc tttgaagtac tgcacggacg cgaagccaag
     3001 gtaacagaaa cagcaagagt accagcacct gttgaaattc ctgttactcc accaactttg
     3061 gtctcgggct taaaaaatgt gactgtcata gaaggtgaat ctgtcacctt ggagtgccac
     3121 atctctggat acccatcccc gacagtgaca tggtacaggg aagactacca aatcgaaagt
     3181 tccattgact tccagataac cttccagagt ggaattgctc gtcttatgat tcgcgaagca
     3241 tttgcggaag acagcgggcg atttacttgc agtgctgtaa atgaggctgg aaccgtcagc
     3301 acatcctgct atctggctgt gcaggtgtca gaagaatttg aaaaggaaac cacagccgtg
     3361 actgagaaat ttactacaga agagaaacgc tttgttgagt caagagatgt ggttatgact
     3421 gatactagcc tcacagagga acaagcaggg cctggagaac ctgccgcgcc ttactttatt
     3481 acaaaaccag tggtccagaa actggtggaa ggtgggagcg tggtgtttgg atgccaagtt
     3541 ggcggcaacc caaagcccca tgtatactgg aaaaaatctg gtgttcctct aaccactgga
     3601 tacagataca aagtgagtta caacaaacaa accggtgaat gcaagctggt gatttctatg
     3661 acttttgctg atgatgctgg agaatacact attgttgttc gcaataagca tggagaaact
     3721 tctgcatctg cttccttgct tgaagaagct gattatgagt tactgatgaa gtcccagcaa
     3781 gaaatgcttt atcagacaca agtgactgca tttgttcaag aacctaaagt tggagaaaca
     3841 gcacctggat ttgtatactc tgagtatgaa aaagagtatg aaaaagaaca agccttaatt
     3901 aggaagaaaa tggccaaaga tactgtagtg gtcagaactt atgtagaaga tcaggaattc
     3961 catatttctt cctttgaaga gagacttatt aaagaaattg aatatagaat aataaagact
     4021 acattagaag aacttcttga agaagatgga gaagaaaaga tggcagttga catttctgaa
     4081 tctgaagctg ttgaatcagg atttgattca agaatcaaga attatagaat tcttgagggg
     4141 atgggtgtca cttttcattg caagatgtct ggatatccat taccaaagat tgcttggtac
     4201 aaagatggca agcgcatcaa acatggagaa agataccaaa tggactttct acaagatggc
     4261 agagctagtc tgcgtatacc tgttgttctt ccagaagatg aaggaatcta cactgcattt
     4321 gccagcaata ttaaaggaaa tgcaatttgc tcagggaaat tgtatgtgga gcctgctgca
     4381 ccacttggag ctccgactta cattcccaca ctagagccag tgagcagaat cagatctctc
     4441 tctccacgtt cagtgagcag gtctcctata cgcatgtctc ctgcacggat gtcacctgca
     4501 aggatgtctc ctgcacggat gtcccctgca agaatgtccc ctggacgtag gctggaggag
     4561 acagatgagt cacaacttga gagactatat aaaccagtct ttgtgttaaa acctgtttct
     4621 ttcaaatgtt tagaagggca aactgccaga tttgacttaa aggttgttgg tagacctatg
     4681 ccagagacgt tctggtttca tgatggccag caaattgtca atgactatac ccataaagta
     4741 gtcattaaag aagatggtac tcaatcacta attattgtcc ctgccacacc cagtgattct
     4801 ggggaatgga ctgtggttgc ccaaaacagg gcaggcagat cttcaatttc agtgatttta
     4861 actgtggaag ctgtggaaca tcaggtaaaa ccgatgtttg tagaaaaact gaaaaatgtc
     4921 aatataaagg aaggttcccg acttgaaatg aaagtcagag ctacgggtaa ccccaaccct
     4981 gacattgtat ggttgaaaaa cagtgacatc attgtgcctc ataaatatcc caaaatcaga
     5041 attgaaggaa ccaagggaga agctgccctt aaaatcgatt ccactgtcag ccaagattct
     5101 gcctggtata ctgcgactgc tattaataaa gctggcagag acactacaag atgcaaagta
     5161 aatgttgaag ttgagtttgc agagcctgag ccagagagaa agttaatcat cccacggggg
     5221 acatatagag caaaggagat tgcagcccca gaactggagc ccctccattt gcgatatggc
     5281 caagagcaat gggaagaagg tgatctctat gacaaagaga aacaacagaa accatttttc
     5341 aagaaaaaac tcacttcctt aagacttaag cgctttgggc ctgcccactt tgaatgcagg
     5401 ctaacaccca ttggtgaccc aacgatggtg gtggagtggc tccatgatgg aaagccactt
     5461 gaagcagcca acaggctccg tatgatcaat gaatttgggt actgcagcct tgattatggc
     5521 gttgcatatt ctagagacag tggtatcatt acttgcagag ccactaacaa atatggaaca
     5581 gatcacacat ctgctaccct tattgttaaa gatgagaaaa gtcttgtgga agaatcccaa
     5641 ttgcctgagg ggaggaaagg cttacagaga attgaagaat tagagagaat ggctcatgaa
     5701 ggtgcactta caggtgtaac aacagatcag aaagaaaagc aaaagccaga cattgtcttg
     5761 tacccagagc cagttagagt acttgaaggg gagactgcaa ggttccgctg cagggtaaca
     5821 ggctaccctc agcccaaagt caactggtac ctcaatggac agctcatccg caaaagcaaa
     5881 aggttcagag ttcgctatga tggtatccat tacctggaca tcgtggactg caaatcatat
     5941 gacacaggtg aagtgaaggt caccgcggaa aatcctgaag gtgtgataga gcataaagtg
     6001 aagcttgaga ttcaacagag ggaagatttt aggtctgtcc ttaggagagc tcctgaacca
     6061 aggcctgagt ttcacgtaca tgaaccagga aagcttcagt ttgaagtaca aaaagtggat
     6121 agacctgttg acaccactga aaccaaagaa gttgtgaagt tgaaaagggc tgaaagaatt
     6181 acccatgaaa aagtgcctga agagtcggaa gagctgcgca gtaaattcaa gcgcagaaca
     6241 gaagagggct attatgaagc cattaccgct gtggagctca agtctcgaaa gaaggatgaa
     6301 tcctatgagg aactcctcag gaagacaaaa gatgaacttc tccactggac caaagagtta
     6361 actgaagagg aaaagaaagc tcttgccgaa gaaggcaaaa tcacgattcc aacttttaaa
     6421 cctgacaaga ttgaactaag tcctagtatg gaggctccaa aaatcttcga aagaatccag
     6481 agccaaacag tgggccaagg atctgatgca cacttccggg tcagagtcgt ggggaaacca
     6541 gaccccgaat gtgaatggta caaaaatggt gtcaaaattg aacggtctga ccggatctac
     6601 tggtactggc ccgaagacaa tgtttgtgaa ttggtcataa gagatgtgac tgctgaggac
     6661 tctgccagca tcatggtaaa agccatcaac atagctggag aaacctccag tcacgcattc
     6721 ttacttgtcc aagccaagca attgatcact ttcacacagg aattacaaga tgttgttgct
     6781 aaggaaaaag acactatggc aacctttgaa tgtgaaactt cagaaccatt tgtcaaagtg
     6841 aaatggtata aagatggtat ggaggttcat gagggagata aatacaggat gcactctgac
     6901 agaaaggttc acttcctctc catactgacc attgatacgt ctgatgctga agattacagc
     6961 tgtgtacttg tggaagatga aaatgtcaaa acgactgcta aacttattgt tgaaggtgca
     7021 gttgttgagt ttgtgaaaga acttcaggac atagaagttc cagaatcata ttcaggagaa
     7081 ttagagtgca ttgtatcccc agaaaatata gaaggaaaat ggtatcataa tgatgtggag
     7141 cttaaatcca atggcaaata tacaattaca tctcgtcgtg gacgtcagaa cctcacggtc
     7201 aaggatgtaa ccaaggagga ccagggagaa tacagctttg tcatcgacgg gaaaaagaca
     7261 acctgtaaat taaagatgaa accccgcccc attgctatcc tacaaggact tagtgaccaa
     7321 aaagtctgtg agggtgacat tgttcagctt gaagttaaag tctccttgga aagtgtggaa
     7381 ggcgtctgga tgaaagacgg ccaagaagtg cagcccagtg acagggttca cattgtgata
     7441 gacaaacaat ctcatatgct gctcattgaa gacatgacta aggaagatgc tggaaattac
     7501 tctttcacca ttccagccct tggcctctcc accagtgggc gtgtctctgt ctatagtgtg
     7561 gacgtgataa cacctctaaa agatgttaat gtgattgaag gcaccaaggc tgtgcttgaa
     7621 tgtaaggtgt cagtccctga tgtgacttct gttaagtggt acttaaatga tgaacaaatc
     7681 aagcctgatg accgtgtaca ggccattgtg aaaggtacta aacagcgact agtcattaac
     7741 cgaactcatg cttcagacga aggaccttac aagctgatag ttggcagagt tgaaaccaac
     7801 tgtaatctct ctgtagaaaa aattaaaatt atcagaggtc ttcgtgacct tacctgtaca
     7861 gaaactcaaa atgtggtgtt tgaggttgag ctgtcccact ctggaattga tgtcctgtgg
     7921 aattttaagg acaaggaaat caagcccagt tctaaatata aaattgaagc acatggaaaa
     7981 atatataaat tgacagttct aaatatgatg aaagatgatg aaggaaaata cacattttac
     8041 gcgggagaaa atatgacatc tggaaaactt actgtggcag gtggggccat ctccaagcca
     8101 ctcacagatc agaccgtagc tgaatcccag gaagctgtgt ttgaatgtga agttgccaac
     8161 ccagattcca aaggcgaatg gttgagggat ggcaaacacc taccactgac taacaacatc
     8221 agaagtgagt ctgatggcca caaaaggaga cttatcattg ctgccaccaa attagatgac
     8281 attggagaat atacctacaa ggtggccacc tccaaaacat ctgccaaact caaagttgaa
     8341 gctgtcaaaa ttaagaagac tctgaagaac ctcacagtga cagaaacaca ggatgctgtt
     8401 ttcactgtcg agcttacaca ccctaatgtc aaaggtgtcc agtggatcaa aaatggagtt
     8461 gtgctggaat ccaatgaaaa gtatgctatc tctgtcaaag gaacaattta ctctctgagg
     8521 attaaaaact gtgccatcgt ggatgagtct gtttatggct tcaggcttgg aaggcttgga
     8581 gccagtgcca gactgcacgt ggagactgtc aagatcatta aaaagccaaa ggatgtgaca
     8641 gccttggaaa atgccactgt tgcctttgaa gttagtgttt cccatgacac tgttccagta
     8701 aaatggttcc ataagagtgt ggaaattaag ccaagtgaca aacacagact ggtctcagaa
     8761 aggaaagtcc acaagctgat gctgcagaac atctccccct cagatgctgg ggaatacaca
     8821 gctgtggtcg ggcaattgga atgcaaagca aaactgtttg tggagacatt acatattaca
     8881 aaaaccatga aaaatatcga ggtgcctgag accaaaactg cctcttttga gtgtgaggtg
     8941 tcccacttca atgtcccttc catgtggctg aagaatggtg tggaaattga gatgagtgaa
     9001 aagttcaaga tagttgtgca gggaaaactc catcagctga tcatcatgaa caccagcaca
     9061 gaggactcgg cagaatacac atttgtctgt ggcaatgacc aagtcagtgc caccctgaca
     9121 gtcaccccaa tcatgattac ttccatgctg aaagacatca acgctgaaga aaaagacact
     9181 attacttttg aggtgacagt gaactatgaa ggcatctctt acaaatggtt aaagaatggt
     9241 gtggaaatca aatcaactga caagtgccag atgagaacca aaaagctcac acactcactg
     9301 aacatcagga atgttcactt tggggatgct gctgactaca cctttgtggc tggaaaagca
     9361 acatcaacag ccacacttta tgtggaagct cgtcatatag aatttaggaa acacattaag
     9421 gacattaagg tactggagaa gaagcgagcc atgtttgaat gtgaagtttc tgaacctgac
     9481 atcactgtac agtggatgaa agatgaccag gaactgcaga tcacagacag aataaagatt
     9541 cagaaggaga aatatgtcca ccgccttctg atcccatcca cccggatgtc tgatgctggg
     9601 aagtacacag tggtggcagg aggcaacgtg tcaactgcaa aactctttgt agaaggcaga
     9661 gatgttcgca tccgaagtat taaaaaggag gttcaggtca ttgagaaaca gcgtgctgtt
     9721 gttgaatttg aggtcaatga agacgatgtt gatgcccact ggtataaaga tggcattgaa
     9781 atcaatttcc aagttcaaga acgacacaaa tatgtagtgg aaagaagaat ccaccgaatg
     9841 tttatctctg agaccagaca gagcgatgca ggagaataca cctttgtggc aggaaggaac
     9901 aggagttctg tcactctcta tgtcaatgct cctgaaccgc cccaagttct gcaggagctc
     9961 cagcctgtca ctgtgcagtc tggcaagcct gcccgcttct gtgccgtgat atccggaaga
    10021 ccacagccca aaatttcctg gtacaaggaa gagcagctgc tttccactgg cttcaagtgc
    10081 aaatttcttc atgatgggca agaatacacg cttttgctaa ttgaagcctt cccagaggat
    10141 gcggcagtct atacctgtga agccaagaat gactatggtg ttgccacaac atcagcttca
    10201 ctctcagtgg aagttccaga agttgtgtct cctgatcagg aaatgcctgt ttatccacct
    10261 gccatcatca ccccgcttca ggacactgtc acttctgaag ggcagccagc ccgttttcaa
    10321 tgccgggttt ctggaacaga tctaaaagtg tcgtggtaca gcaaagacaa gaaaatcaag
    10381 ccatctcggt tctttagaat gactcaattt gaagacactt atcaactgga aattgccgaa
    10441 gcttatccag aagatgaagg aacttacacg tttgttgcta gtaatgctgt aggccaagta
    10501 tcaagcacag ccaacctgag tctggaagct cctgaatcaa ttttgcatga gaggattgaa
    10561 caagagattg agatggaaat gaaagaatta ttctctgagg gggaatctga acattcagaa
    10621 agggatacac gtgacgcctt ttcagattct gaagacatag accacaaatc catggctgct
    10681 aaaagatatg caagtagaat atcatctaca agctcctggc ctgaatattt taagccttct
    10741 tttacccaga aacttacatt taaatatgtt ttagagggtg aaccggttgt ttttacatgt
    10801 agattaattg cctgtccaac tccagaaatg acttggtttc ataacaatcg gcccatccca
    10861 acaggtcttc gtcggattat aaaagctgag agtgatttac accatcactc ttccagctta
    10921 gagattaaaa gggtccaaga cagagattct ggaagttata ggttactagc aattaatagt
    10981 gaagggtctg cagaatcaac tgcatcattg cttgttatcc aaaaagggca agatgagaaa
    11041 tatttagaat ttctgaaaag agcagaacgg acacatgaaa atgtggaggc tttagttgaa
    11101 aggggagaag ataggataaa ggttgacctg aggtttactg gctctccctt taacaaaaaa
    11161 caagatgtgg aacaaaaggg aatgatgaga actatacatt ttaagacaat gagttctgcc
    11221 aagaaaacag attacatgta tgatgaagaa tatttagaaa gtaaatctga cataagaggt
    11281 tggcttaatg taggtgaaag tttcctggac aaggagacca aagtgaagtt gcaacgttta
    11341 agggaggcta gaaaaacatt aatggagaaa aagaaattat ctcttttaga tacgtcttct
    11401 gaaataagct caagaacttt gagaagtgaa gctagtgata aagatattct gttctctaga
    11461 gaggacatga aaatcagatc tatgtctgat ctagctgaaa gttataaggt agatcattca
    11521 gctgagagta ttgtgcaaaa tccacatgcc ctttctaatc aaatggatca aaatatagaa
    11581 tctgaggaac ttcccacaag ctttcaaact attgttgatg aagaaatttt ccagactgaa
    11641 ataagaatga gccaagaagc actggtaaag gaaagtctac caaaagacca tttatatggt
    11701 gaaattctag taaatgaaaa tacccaagca agaggacagc tggaagaaat tatggccaat
    11761 actacaattg gagagtcatc cacatatatc actaatgtat gtgagaagga ggaagtatat
    11821 gagactcctg aaaatgtgtc tcaagctatt acaccacatg ccagcgaatc ttttggtaca
    11881 cttgtaaatg ttgaagaaag tgaagaaata gctagtgaaa gaataaaaaa ggatgatttg
    11941 agagaattac aactctctgc ttctacacga attgatgaat tcaaaaccga acagaaggaa
    12001 gaaaacatga gattttttga aaattctttt cgaaaacgcc cacaacgttg cccaccttca
    12061 tttcttcaag aaattgaatc tcaagaagtt tatgaaggtg acagttgtaa ttttgtgtgt
    12121 cattttcaag gatatcctca acctatagtg acgtggtata acaatgacat gccaatccca
    12181 cgtaatcaaa actttatcat tcattcgttg gaaaactatt caatattaac tctttcatct
    12241 gttcatcatc agaatgaagg ttctattact tgtgtgctat ttaaccaata tggaacagta
    12301 aaaacaacca gtatgcttaa agtgaaagca aaacaaaaac atgatgtcaa agcacataaa
    12361 gtcccagtgt tccatgacta cttggatgag gaagaagagt tagcattggt gtttgaccaa
    12421 gcaaaaggcg cccatccatc tatgagtcaa gagggccaaa caaatcttca tttgttaaaa
    12481 actaacccac cagttcctcc ctctggagac acagaattgc tttcctttcc tgtagaaatt
    12541 caggtaacag cagctactcc tatcccagaa caggataaag agtctaaaga agtgtttcaa
    12601 actgaggagc tggaacctaa agcaatgcct caagatcaag ttactcaatc accaaaacac
    12661 agatttgtat ttttgtctga tattactaat gaaccaccaa aaatgttaca agaaatgcca
    12721 aagcatgcca ggtgtagaga aggggattcc atcattcttg aatgtttaat atctggtgag
    12781 ccccagccag ttgtaacatg gtttcaaaat ggagtccttt taaagcagaa ccagaagttt
    12841 cagtttgaag aagttaattg tagccaccaa ttatatatta aggatgttaa ttctcaagat
    12901 tctggaaaat ataaatgtgt tgctgaaaac aattcagggg cagttgaaag tgtttcagat
    12961 ctaacagtgg agcctgtcac ttatagagaa aatagtcaat tcgaaaatat tggtgaaatt
    13021 tatggaaaat attccagaga tcagcaactg caagaccagg gagaatctgt aagggcacat
    13081 ttttatgatt atccagctgg tccctttact ccctggacaa atgtaaaaga atattcagta
    13141 agggactatt ttcaaagcct tgagactatt gagcaaatag accagaaaga gcaggtgcgt
    13201 tgcatacctt ctagagaaaa aattcccaga tttgtgcatg gtgcttcaag aaccataaaa
    13261 atcagcaagc ccatcagggc tgaattcata caatgtcagg ctgaagggaa agagagacat
    13321 gtaagtgaaa aatcaaagct gcaccaagca gagggtactg tttatccatt tgttgatgat
    13381 ttcagtgatg tcactattaa gaaagaaata agaaataatt ttggaaaact tggtagatca
    13441 gaaaaggaaa atgtacaaga atgtgcccaa agtgactatt taccaaatat tcactctgag
    13501 agaatttctg acagttacaa tacaaaagat tcatctgcca ttgtatatga agaatccctt
    13561 ggtgaagaga tacactaccc ggggaaaaag gtgaagcata gaattatcga atttgaaaaa
    13621 ttacatgtag aaaaaggagt tctagaaaaa agacctacca gaacatcaat tgttaatcct
    13681 cctcaaaaga agattgatga caaagccttt tcattaaagc aaagagaatc aagatcaagc
    13741 aatctaaatg caaatatgta tcaggcagag aaaatgtctc caaatactga atcagattca
    13801 tctaacattg caataaattt aaagctgctt tcctctcaaa ctcataaaga atttgacgca
    13861 caagaaaggg aacaacagga aaaaataagt cttatagata aacctgcaat ttctaaaaga
    13921 gctgaacatg aatcacctat tacatttgac ttaaagcagt ttcacactca aataaaacac
    13981 acagatgtga aattccaaga attagatagt ggtcaaccag aagaagctta ttttaaaatc
    14041 caacatcctg ctgacactga gaacattgta tttgatctaa aacaaatgta ttctcatata
    14101 ggagatccag ctctggagtt ccaagggcaa gaaactaggg aacagcagga aatacattat
    14161 aaagaaaaaa ttcctagccc tgaaacgtta caacctgaca cacacaatat ctctaaaagt
    14221 gtgcaaaata atgtatttgc cagtcaagaa atttcttcca gtcaagagct ttcaaataga
    14281 actatggtgg agaaaagtag tattgatgaa aattccattt ctttagaaaa agaagtccga
    14341 catgtacagg aacaaaactt agacatttta aaaactgatc tttctcttaa atccttttct
    14401 gaagaaatct atagtgaatc atgtgcccta cttccaacct catctgcaga tatagaagaa
    14461 actgatcttt ctgagaaaag ttgtcctcta gaaaatggag gcagaagttc catctcacat
    14521 ttgaaaaaag cagcaagtga ggaaaagcct ttaggggttg gtgaaatgga agaagaatgc
    14581 actctagaac cagagttggc agcatttcca aagcaggatg gtgggacaca ggaatataca
    14641 gatgccactt tggaagacca cagaggtgat gttcaggaag ctgacacact ccatagacag
    14701 ctctccctga gtcagtgctt cccactcttg atgactgaag aacaacagaa tccaggtgaa
    14761 caaataagta caaacattca tgcctctgga gaagaaaaat gttatgaaga agttcaagtt
    14821 cagaatgaag cttctttctc cactttagaa ggagaaatga tagaaactag tttttctcaa
    14881 aatattccta aattagatga ggcacatacc actgaggcag cagaatctga gacctcccta
    14941 acacaatatt tacttgctgc tggaaagcgt gaggttcctg aaactaaaga caccagagac
    15001 caggcaaagc ttgtccagag tgaatcaatc acttcaatgg aggtggaaga agtaactttc
    15061 aacactgtgt atgaatacta caaccagaaa caggaatcac taggtcgtcc actttctcct
    15121 gagtctgaca tttccattgg tgtgggaagt acaaccagtg aagaaatatc tgagttagat
    15181 cagttttata ccccaccatc atctgttgaa tattttgaat ctccaaagtc ccctgactta
    15241 tattttaatc cttcagatat aactaaacaa tcctcaatac attcaggagg tgagacagtt
    15301 gaaagatatt ccacaccttt aggggaagtt gctgaaagat attcaacacc ttccgaaggt
    15361 gaggtaggag aaagatactc cacaccccca ggggagacac tagagagata ctccacacct
    15421 ccaggagaga ctctagagag atactccaca cccccaggag agactctaga gagatattcc
    15481 acacccccag gagagactct ggagagatac tccacacccc caggagagac tctagagcga
    15541 tattccacac ccccagggga ggcactagag agatattcta ttcctactgg aggaccaaac
    15601 cccactggta cttttaaaac atatccatca aaaatagaaa gggaagacgg tacaccaaat
    15661 gagcatttct acacacctac agaagagagg ggttcagctt atgaaatatg gcgttccgat
    15721 tcatttggta cacccaatga agccattgag ccaaaagaca atgaaatgcc tccatctttt
    15781 attgaacctc tgaccaaaag gaaggtatat gaaaacacaa cactaggctt cattgttgaa
    15841 gttgaaggtc ttccagttcc tggtgtgaaa tggtatcgaa ataaatcttt actagagcca
    15901 gatgaaagaa tcaaaatgga aagagtgggt aatgtgtgtt cactggaaat ttctaacatt
    15961 caaaaaggag aagggggaga gtacatgtgt catgctgtaa acatcatagg ggaagcaaag
    16021 agctttgcaa atgtagacat aatgccccag gaagaaagag tggtggcact accacctcca
    16081 gtaacacatc agcatgtcat ggagtttgat ttggaacaca ccacatcatc aagaacacct
    16141 tctcctcaag aaattgtcct ggaagttgaa ttaagtgaaa aagacgttaa agaatttgag
    16201 aagcaggtga aaatagtgac agttcccgaa tttactcctg accataaaag tatgattgtg
    16261 agtctagatg ttcttccatt taattttgta gatccaaata tggattcaag ggagggagaa
    16321 gacaaagaac taaaaattga tttagaagta tttgaaatgc ctcctcgctt tataatgcct
    16381 atttgtgatt ttaaaattcc agaaaattca gatgctgtat tcaaatgttc agtcataggg
    16441 atcccgactc ccgaagttaa gtggtataaa gaatatatgt gtattgagcc agataatatt
    16501 aaatacgtga ttagcgagga gaagggaagt cacactctta aaattcgaaa tgtctgtctt
    16561 tctgatagtg caacatacag gtgcagagct gtgaattgtg taggagaggc tatctgtcgg
    16621 ggattcctca ccatgggaga ttctgaaata tttgctgtga tagcaaagaa aagcaaagtg
    16681 actttaagca gtttaatgga agaattggtc ttaaagagca actacacaga cagttttttt
    16741 gaatttcagg tggtggaagg gcctcccagg tttatcaaag gtatttctga ctgttatgca
    16801 ccaataggta cagcagcata ttttcagtgc ttagttcgtg gctctccaag acccacggtt
    16861 tactggtaca aagatggaaa attagtccaa ggaagaaggt tcactgttga ggaaagtggc
    16921 acagggttcc ataacctgtt tataacaagc ttagtaaaga gtgatgaagg agagtatagg
    16981 tgtgtagcta caaacaaatc aggaatggct gagagctttg cagcactcac cttaacttaa
    17041 aatgtaatgt tttagtgcct cagtaattat tagcattgat ctgagtgctt tcatattttc
    17101 caaattatgt ggatctaata aacttccaaa caggtccacc atatttgaat tcattacctt
    17161 ggagacccct aaagaaataa tctctatgta gaaatctcat ctttgtaata catgtaaata
    17221 ttttgttatc tgaactgtgg aatcatcact tgtgtcaatc atgctgtgta atatcaaaca
    17281 caattaaatc tctccttggc tacctaattg tttgtacttc tatttaccct catgcctcct
    17341 ccacaaagtg tttcagtgga atggactctc ttttagcaga taaaccatat atgcaaaagt
    17401 acaatgtaaa agtggagacc atatcagatg accacgcaga caaattggta agggcaaatc
    17461 tctctgctcc cattctaagt tatttttgga attttaaccc ctttgcatcc aaactcatgt
    17521 ttatttactt tgacttagaa taggcaaaac aaacaacaaa aaccagcttc tcccaattgg
    17581 caactaactc tataatctgt ttgacatttc aatcttaata tattaacttc atctctttta
    17641 aaaatgtttt cgttattaat attcctcttc ataagtgctt taattctcac tggcttcaag
    17701 ggaccaattg tgctctttgt gggcagggtt cacctagaca tttgcaaaat aagcaaatag
    17761 atctttccac ttttggagat accttatttt tctccatttt tgagatgaca tcaagtgtaa
    17821 aatgcagcaa taatttagta ttggcctggg gaaaaaaggg aaactatcat attgtgttta
    17881 catatagatt ttaaaactgt attctgattt cagaatcttt aaaatttaaa aaaaggtggg
    17941 atatttttag aattgagaca atatggtaat ttgtatttca gattatatat aaataacaag
    18001 ataacaagct ttcctatcag tttcactgaa tttcatcttt aaaacgaata tagcttaata
    18061 agcattcaat ttttattgtg atgtttatgt ttctaaagta actctttaat tttgtctttt
    18121 aaaattaaaa taacagtaaa ttaacatact ccttaatatc ttacctatat actaatgtgg
    18181 acccaagtct tctcatataa gactcgacaa atataaaaaa
//
