LOCUS       NM_014640               5007 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens tubulin tyrosine ligase like 4 (TTLL4), mRNA.
ACCESSION   NM_014640
VERSION     NM_014640.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Arnold,J., Schattschneider,J., Blechner,C., Krisp,C., Schluter,H.,
            Schweizer,M., Nalaskowski,M., Oliveira-Ferrer,L. and Windhorst,S.
  TITLE     Tubulin Tyrosine Ligase Like 4 (TTLL4) overexpression in breast
            cancer cells is associated with brain metastasis and alters exosome
            biogenesis
  JOURNAL   J Exp Clin Cancer Res 39 (1), 205 (2020)
   PUBMED   32998758
  REMARK    GeneRIF: Tubulin Tyrosine Ligase Like 4 (TTLL4) overexpression in
            breast cancer cells is associated with brain metastasis and alters
            exosome biogenesis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Mahalingan,K.K., Keith Keenan,E., Strickland,M., Li,Y., Liu,Y.,
            Ball,H.L., Tanner,M.E., Tjandra,N. and Roll-Mecak,A.
  TITLE     Author Correction: Structural basis for polyglutamate chain
            initiation and elongation by TTLL family enzymes
  JOURNAL   Nat Struct Mol Biol 27 (9), 870 (2020)
   PUBMED   32792670
  REMARK    Correction to:[Nat Struct Mol Biol. 2020 Sep;27(9):802-813. doi:
            10.1038/s41594-020-0462-0.. PMID: 32747782]
REFERENCE   3  (bases 1 to 5007)
  AUTHORS   He,M., Xu,M., Zhang,B., Liang,J., Chen,P., Lee,J.Y., Johnson,T.A.,
            Li,H., Yang,X., Dai,J., Liang,L., Gui,L., Qi,Q., Huang,J., Li,Y.,
            Adair,L.S., Aung,T., Cai,Q., Cheng,C.Y., Cho,M.C., Cho,Y.S.,
            Chu,M., Cui,B., Gao,Y.T., Go,M.J., Gu,D., Gu,W., Guo,H., Hao,Y.,
            Hong,J., Hu,Z., Hu,Y., Huang,J., Hwang,J.Y., Ikram,M.K., Jin,G.,
            Kang,D.H., Khor,C.C., Kim,B.J., Kim,H.T., Kubo,M., Lee,J., Lee,J.,
            Lee,N.R., Li,R., Li,J., Liu,J., Longe,J., Lu,W., Lu,X., Miao,X.,
            Okada,Y., Ong,R.T., Qiu,G., Seielstad,M., Sim,X., Song,H.,
            Takeuchi,F., Tanaka,T., Taylor,P.R., Wang,L., Wang,W., Wang,Y.,
            Wu,C., Wu,Y., Xiang,Y.B., Yamamoto,K., Yang,H., Liao,M., Yokota,M.,
            Young,T., Zhang,X., Kato,N., Wang,Q.K., Zheng,W., Hu,F.B., Lin,D.,
            Shen,H., Teo,Y.Y., Mo,Z., Wong,T.Y., Lin,X., Mohlke,K.L., Ning,G.,
            Tsunoda,T., Han,B.G., Shu,X.O., Tai,E.S., Wu,T. and Qi,L.
  TITLE     Meta-analysis of genome-wide association studies of adult height in
            East Asians identifies 17 novel loci
  JOURNAL   Hum Mol Genet 24 (6), 1791-1800 (2015)
   PUBMED   25429064
REFERENCE   4  (bases 1 to 5007)
  AUTHORS   Ahmeti,K.B., Ajroud-Driss,S., Al-Chalabi,A., Andersen,P.M.,
            Armstrong,J., Birve,A., Blauw,H.M., Brown,R.H., Bruijn,L., Chen,W.,
            Chio,A., Comeau,M.C., Cronin,S., Diekstra,F.P., Soraya Gkazi,A.,
            Glass,J.D., Grab,J.D., Groen,E.J., Haines,J.L., Hardiman,O.,
            Heller,S., Huang,J., Hung,W.Y., Jaworski,J.M., Jones,A., Khan,H.,
            Landers,J.E., Langefeld,C.D., Leigh,P.N., Marion,M.C.,
            McLaughlin,R.L., Meininger,V., Melki,J., Miller,J.W., Mora,G.,
            Pericak-Vance,M.A., Rampersaud,E., Robberecht,W., Russell,L.P.,
            Salachas,F., Saris,C.G., Shatunov,A., Shaw,C.E., Siddique,N.,
            Siddique,T., Smith,B.N., Sufit,R., Topp,S., Traynor,B.J., Vance,C.,
            van Damme,P., van den Berg,L.H., van Es,M.A., van Vught,P.W.,
            Veldink,J.H., Yang,Y. and Zheng,J.G.
  CONSRTM   ITALSGEN consortium; ALSGEN Consortium
  TITLE     Age of onset of amyotrophic lateral sclerosis is modulated by a
            locus on 1p34.1
  JOURNAL   Neurobiol Aging 34 (1), 357 (2013)
   PUBMED   22959728
REFERENCE   5  (bases 1 to 5007)
  AUTHORS   Rogowski,K., van Dijk,J., Magiera,M.M., Bosc,C., Deloulme,J.C.,
            Bosson,A., Peris,L., Gold,N.D., Lacroix,B., Bosch Grau,M., Bec,N.,
            Larroque,C., Desagher,S., Holzer,M., Andrieux,A., Moutin,M.J. and
            Janke,C.
  TITLE     A family of protein-deglutamylating enzymes associated with
            neurodegeneration
  JOURNAL   Cell 143 (4), 564-578 (2010)
   PUBMED   21074048
REFERENCE   6  (bases 1 to 5007)
  AUTHORS   Kashiwaya,K., Nakagawa,H., Hosokawa,M., Mochizuki,Y., Ueda,K.,
            Piao,L., Chung,S., Hamamoto,R., Eguchi,H., Ohigashi,H.,
            Ishikawa,O., Janke,C., Shinomura,Y. and Nakamura,Y.
  TITLE     Involvement of the tubulin tyrosine ligase-like family member 4
            polyglutamylase in PELP1 polyglutamylation and chromatin remodeling
            in pancreatic cancer cells
  JOURNAL   Cancer Res 70 (10), 4024-4033 (2010)
   PUBMED   20442285
REFERENCE   7  (bases 1 to 5007)
  AUTHORS   van Dijk,J., Miro,J., Strub,J.M., Lacroix,B., van Dorsselaer,A.,
            Edde,B. and Janke,C.
  TITLE     Polyglutamylation is a post-translational modification with a broad
            range of substrates
  JOURNAL   J Biol Chem 283 (7), 3915-3922 (2008)
   PUBMED   18045879
REFERENCE   8  (bases 1 to 5007)
  AUTHORS   Trichet,V., Ruault,M., Roizes,G. and De Sario,A.
  TITLE     Characterization of the human tubulin tyrosine ligase-like 1 gene
            (TTLL1) mapping to 22q13.1
  JOURNAL   Gene 257 (1), 109-117 (2000)
   PUBMED   11054573
  REMARK    GeneRIF: Characterization of another human tubulin tyrosine
            ligase-like gene family member
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC009974.10, DB097392.1 and AK291770.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014640.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The extent of this RefSeq
            transcript is supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D79995.1, SRR1803611.163390.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392102.6/ ENSP00000375951.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AC009974.10        48919-48928
            11-276              DB097392.1         8-273
            277-3984            AK291770.1         225-3932
            3985-5007           AC009974.10        92478-93500
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q35"
     gene            1..5007
                     /gene="TTLL4"
                     /note="tubulin tyrosine ligase like 4"
                     /db_xref="GeneID:9654"
                     /db_xref="HGNC:HGNC:28976"
                     /db_xref="MIM:618738"
     exon            1..203
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            204..282
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            283..1867
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     CDS             381..3980
                     /gene="TTLL4"
                     /note="tubulin polyglutamylase TTLL4; tubulin--tyrosine
                     ligase-like protein 4; tubulin tyrosine ligase-like
                     family, member 4; protein monoglutamylase TTLL4"
                     /codon_start=1
                     /product="tubulin monoglutamylase TTLL4"
                     /protein_id="NP_055455.3"
                     /db_xref="CCDS:CCDS2422.1"
                     /db_xref="GeneID:9654"
                     /db_xref="HGNC:HGNC:28976"
                     /db_xref="MIM:618738"
                     /translation="MASAGTQHYSIGLRQKNSFKQSGPSGTVPATPPEKPSEGRVWPQ
                     AHQQVKPIWKLEKKQVETLSAGLGPGLLGVPPQPAYFFCPSTLCSSGTTAVIAGHSSS
                     CYLHSLPDLFNSTLLYRRSSYRQKPYQQLESFCLRSSPSEKSPFSLPQKSLPVSLTAN
                     KATSSMVFSMAQPMASSSTEPYLCLAAAGENPSGKSLASAISGKIPSPLSSSYKPMLN
                     NNSFMWPNSTPVPLLQTTQGLKPVSPPKIQPVSWHHSGGTGDCAPQPVDHKVPKSIGT
                     VPADASAHIALSTASSHDTSTTSVASSWYNRNNLAMRAEPLSCALDDSSDSQDPTKEI
                     RFTEAVRKLTARGFEKMPRQGCQLEQSSFLNPSFQWNVLNRSRRWKPPAVNQQFPQED
                     AGSVRRVLPGASDTLGLDNTVFCTKRISIHLLASHASGLNHNPACESVIDSSAFGEGK
                     APGPPFPQTLGIANVATRLSSIQLGQSEKERPEEARELDSSDRDISSATDLQPDQAET
                     EDTEEELVDGLEDCCSRDENEEEEGDSECSSLSAVSPSESVAMISRSCMEILTKPLSN
                     HEKVVRPALIYSLFPNVPPTIYFGTRDERVEKLPWEQRKLLRWKMSTVTPNIVKQTIG
                     RSHFKISKRNDDWLGCWGHHMKSPSFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQ
                     SRFGKKEFSFFPQSFILPQDAKLLRKAWESSSRQKWIVKPPASARGIGIQVIHKWSQL
                     PKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSM
                     KSLGNKFMHLTNYSVNKKNAEYQANADEMACQGHKWALKALWNYLSQKGVNSDAIWEK
                     IKDVVVKTIISSEPYVTSLLKMYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLH
                     SSSPLDISIKGQMIRDLLNLAGFVLPNAEDIISSPSSCSSSTTSLPTSPGDKCRMAPE
                     HVTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRILVEMEDEFSRRGQFERIFPSH
                     ISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRSWCYKGFHMGVVSDSAPVWS
                     LPTSLLTISKDDVILNAFSKSETSKLGKQSSCEVSLLLSEDGTTPKSKKTQAGLSPYP
                     QKPSSSKDSEDTSKEPSLSTQTLPVIKCSGQTSRLSASSTFQSISDSLLAVSP"
     exon            1868..1977
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2041
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2166
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2277
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2354
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            2355..2546
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            2547..2629
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            2630..2758
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            2759..2881
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            2882..2980
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            2981..3115
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3116..3253
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3254..3356
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3357..3567
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3568..3638
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3639..3724
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3725..5007
                     /gene="TTLL4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4256..4261
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TTLL4"
                     /note="hexamer: TATAAA"
     polyA_site      4278
                     /gene="TTLL4"
                     /note="major polyA site"
     regulatory      4984..4989
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TTLL4"
                     /note="hexamer: AGTAAA"
     polyA_site      5007
                     /gene="TTLL4"
ORIGIN      
        1 ctcttcttcc agactctcgg tctgtccgct gggggcgcgc gcggtgtgtg gcaggcggca
       61 gcggcgctgg cggccgagtg cgcttgtcac gcgtggcggt gcgtggttgc taggggcgcc
      121 tgaggctgcc gggtagccca gcaggccgag ggaggaagta gcgtggagcc ggtgccgagc
      181 cggggcgaag ctggatcccc tagatagact gtcttcaagc tcactgatat tttcctctgc
      241 ttgatccatt gtgctgttga gagcctctag taaatttttc agactgacag acttcaagga
      301 tgcagctgct actaccggag gtgtgtggca ccttacctca gcaaggccat gagaccgtgt
      361 ggccatgatg tgggcccctc atggcctcag caggaacaca gcactatagt attggcctcc
      421 gccagaaaaa cagcttcaag cagagtggtc cctcaggcac agtacctgcc acgccacctg
      481 agaaaccctc ggagggcaga gtctggcctc aggcccatca gcaagtgaag ccaatctgga
      541 agctggaaaa gaagcaagtg gagacactgt cagcagggtt gggcccaggc ctcttgggcg
      601 tcccacccca gccagcatat ttcttttgcc ccagcacttt atgtagctct gggaccacgg
      661 ctgtcattgc aggccacagc agttcctgtt acctacactc tctcccggac ttgttcaaca
      721 gcaccctgct ataccgccgc tccagctata ggcaaaaacc gtaccagcaa ctggagtctt
      781 tctgcttgcg ttcgagcccg tcagaaaaaa gccctttttc tctccctcaa aagagcctcc
      841 ctgtcagtct cactgccaac aaggccactt cttccatggt cttctccatg gcccagccca
      901 tggcctcctc atccacagaa ccatacctct gcttggcagc ggctggggaa aacccttcag
      961 ggaagagcct ggcctctgcc atctcaggga agatcccatc tccactctct tcctcctata
     1021 agcccatgct gaataataat tccttcatgt ggccaaatag cacgccagtg cctttattgc
     1081 agaccacaca gggcctgaag ccagtatcgc cacccaagat ccagcctgtc tcctggcatc
     1141 attcaggggg tactggagac tgtgcaccgc agcctgttga ccataaggtg cccaaaagca
     1201 ttggcactgt cccagctgat gccagtgccc atatcgcctt gtctaccgct agctcccacg
     1261 acacatccac caccagtgtt gcctcttcct ggtataaccg gaataactta gccatgaggg
     1321 cagagccact ttcctgtgct ctggatgaca gctctgattc ccaggatcca actaaggaga
     1381 ttcggttcac tgaggccgtg aggaaattga ccgcaagagg ctttgagaag atgccgaggc
     1441 aaggctgcca gcttgaacag tctagtttcc tgaaccccag cttccagtgg aatgtcctca
     1501 acaggagcag gcggtggaaa cctcctgcgg taaatcagca gtttcctcag gaggatgctg
     1561 gatcggtcag gcgggtcctc cctggtgcct cagatacctt ggggttggac aatacagtct
     1621 tctgtaccaa gcgtatcagc attcacctcc ttgcctcaca tgccagtggg ctcaatcaca
     1681 accctgcctg tgaatctgta attgactcct cagcatttgg agaaggcaaa gctccaggtc
     1741 ccccttttcc tcaaactctt ggcatagcca acgtggccac ccgcctctct tccatccagc
     1801 tgggccagtc tgagaaggag agacctgagg aggccaggga gctggactca tctgataggg
     1861 atattagttc agctactgac ctccagccag atcaggctga gactgaagat acagaagaag
     1921 aactagtaga tggtttggaa gactgttgta gccgtgatga gaatgaagag gaggagggag
     1981 actcagagtg ctcctcatta agtgctgtct cccccagcga atcggtggcc atgatctcta
     2041 gaagctgtat ggaaattctg accaaacccc tttccaatca tgagaaagtt gtccgaccag
     2101 ccctcatcta cagtctcttt cccaacgttc cccctaccat ctattttggc actcgggatg
     2161 agagagtgga gaaacttccc tgggaacaga ggaagttgct ccgatggaag atgagcacag
     2221 tgacccccaa cattgtcaag cagaccattg gacggtccca cttcaaaatc agcaaaagaa
     2281 acgatgactg gctgggctgc tggggtcacc acatgaagtc tcctagtttc cgatccattc
     2341 gagagcatca gaagctaaac catttcccag gctcattcca gattgggagg aaggaccggc
     2401 tatggcggaa cctgtcacgt atgcagagcc gctttggcaa gaaggagttc agtttcttcc
     2461 cccagtcctt tatcctgccc caggacgcca agctcctgcg caaagcgtgg gagagcagca
     2521 gccgccaaaa gtggattgtg aagccaccag catcagctcg aggcattggc atccaggtta
     2581 ttcacaagtg gagtcagctc cccaagcgaa ggcccctcct ggtacagagg tatctacaca
     2641 aaccctacct catcagcggc agcaagtttg acctgcggat ctatgtttat gtcacttcct
     2701 acgatcctct gcggatttac ctcttttcag atggactggt ccgctttgcc agttgcaagt
     2761 attcgccttc catgaagagc cttggcaata agttcatgca cctgaccaac tacagtgtca
     2821 ataaaaagaa tgccgagtac caggccaatg cagatgaaat ggcttgccag ggccacaaat
     2881 gggcactgaa ggctttgtgg aactacctga gccagaaggg agtcaatagc gacgccatct
     2941 gggagaagat aaaggatgtt gttgtcaaaa ctatcatctc gtcagagccc tatgtgacca
     3001 gcctgctcaa gatgtatgtg cgacggccct atagctgcca tgaactcttt ggttttgaca
     3061 tcatgctaga cgaaaacctc aagccctggg tcctggaagt caacatttcc ccaagcctcc
     3121 actccagctc tccactggat atcagcatca aaggccagat gattcgtgac cttctgaatc
     3181 tggcaggttt tgtcctgccc aatgcagagg atatcatttc cagccccagc agctgcagca
     3241 gctccaccac cagcctgccc acctcccctg gggacaaatg tcgaatggct ccagagcatg
     3301 tcactgcaca gaagatgaag aaagcctatt atctgaccca gaaaattcct gatcaggact
     3361 tctatgcatc tgtgctggat gtcctgacac cagatgatgt tcggattctg gttgagatgg
     3421 aagatgagtt ttctcgccgt ggtcagtttg aacgaatttt tccttctcat atctcctctc
     3481 gctatctccg cttttttgag cagccacgat atttcaacat tctcaccacc caatgggaac
     3541 agaaatacca tggcaacaag cttaaaggag tagatctgct ccggagttgg tgctacaaag
     3601 ggttccacat gggagttgtc tctgattctg ctccagtgtg gtctctcccg acatcacttc
     3661 tgactatctc aaaggatgac gtgatactca atgccttcag caaatcagag actagcaagc
     3721 tgggaaaaca aagctcctgt gaggttagcc tactactctc tgaagacggg accacgccca
     3781 aatccaagaa gactcaagct ggcctttccc cttatcccca gaaacccagt tcctcaaagg
     3841 acagtgagga caccagcaaa gagcccagcc tttctaccca gacgttacct gtgatcaagt
     3901 gctctgggca gacttcaaga ctttctgctt cctccacttt ccagtcaatc agtgactccc
     3961 tcctggctgt gagcccataa ctggcctctc tccaaaagcc tctgcccagg agcatgggca
     4021 tcagctacct cacgggaacc agcctgctgt tcagaccagt ctgaccccct acccctttca
     4081 ccctgtccct cctcagagta ttttttgaag tggttgcatt atagagatgg gtatttgtag
     4141 ggccggaggg atggtagtga tggggagaag gtgaggaagg gtcaccctct gtcacctgtc
     4201 tgcctggctg gcacctcata tctcagcaga gaagccagtg gtggccacgc agccttataa
     4261 agcaggtttt ggtttctacc ttaagtgagc catgtgtggt ttgtctgggg gccctggtgt
     4321 ggttgctgag ttgtagctca agaggagaaa acatacagaa catatttgga ccggaaatcc
     4381 tttgttctga atttgagggg gtcttctgag gtccttactt ccttaggtct ttcctcaccc
     4441 ctctcccacc gctgtcctga ggagaaaccc ttgaacttcc tcagtagaca ggcggagagg
     4501 ccacaacatg ccgaacccat ttcctgtcat cctagtcttg ggtcttcacc gcctccttcc
     4561 aaatacccac cctgccagca gccctaggtc ttcctgttct gaccccccat cactgctcgt
     4621 tcagccttct agatgtctct ctcgtggaca tctgttcttt agctgttggc tttctctgag
     4681 gtgtgagagg gtctatgaac tttgtgaatt tcccatggcc ccagtgaagg agcccagata
     4741 atcccagtag ctgttacctg tctccatgta tcaaaggaca cagtccaggg ggagggtgga
     4801 aggagatgtg gtttctctat agtgcaacaa acatggtttc tcaatgttct gctgtgcagc
     4861 aagcagggtc tggcggcttg gtaggtgggt ttcaggagca gtcactattg taggatgggc
     4921 ttccaatcaa acctcagact aaactcttgt actgaactga ttctacctcc ctcctctaga
     4981 ctcagtaaac agtgactatt caataaa
//
