LOCUS       NM_003318               2966 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens TTK protein kinase (TTK), transcript variant 1, mRNA.
ACCESSION   NM_003318
VERSION     NM_003318.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Lee,C.C., Tsai,K.Y., Lee,A.W., Wei,P.L., Huang,C.Y., Batzorig,U.
            and Chang,Y.J.
  TITLE     CWH43 Is a Novel Tumor Suppressor Gene with Negative Regulation of
            TTK in Colorectal Cancer
  JOURNAL   Int J Mol Sci 24 (20), 15262 (2023)
   PUBMED   37894942
  REMARK    GeneRIF: CWH43 Is a Novel Tumor Suppressor Gene with Negative
            Regulation of TTK in Colorectal Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Ma,J.J., Xiang,C. and Wang,J.W.
  TITLE     TTK is a potential regulator of tumor progression correlated with
            dedifferentiation and immune cell infiltration in papillary thyroid
            cancer
  JOURNAL   Aging (Albany NY) 15 (19), 10607-10626 (2023)
   PUBMED   37815894
  REMARK    GeneRIF: TTK is a potential regulator of tumor progression
            correlated with dedifferentiation and immune cell infiltration in
            papillary thyroid cancer.
REFERENCE   3  (bases 1 to 2966)
  AUTHORS   Yu,J., Gao,G., Wei,X. and Wang,Y.
  TITLE     TTK Protein Kinase promotes temozolomide resistance through
            inducing autophagy in glioblastoma
  JOURNAL   BMC Cancer 22 (1), 786 (2022)
   PUBMED   35850753
  REMARK    GeneRIF: TTK Protein Kinase promotes temozolomide resistance
            through inducing autophagy in glioblastoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2966)
  AUTHORS   Gao,Y.H., Qu,S.S., Cao,L.Q. and Yao,M.
  TITLE     TTK predicts triple positive breast cancer prognosis and regulates
            tumor proliferation and invasion
  JOURNAL   Neoplasma 69 (2), 274-282 (2022)
   PUBMED   34846157
  REMARK    GeneRIF: TTK predicts triple positive breast cancer prognosis and
            regulates tumor proliferation and invasion.
REFERENCE   5  (bases 1 to 2966)
  AUTHORS   Yao,Z.P., Zhu,H., Shen,F. and Gong,D.
  TITLE     Hsp90 regulates the tumorigenic function of tyrosine protein kinase
            in osteosarcoma
  JOURNAL   Clin Exp Pharmacol Physiol 49 (3), 380-390 (2022)
   PUBMED   34767669
  REMARK    GeneRIF: Hsp90 regulates the tumorigenic function of tyrosine
            protein kinase in osteosarcoma.
REFERENCE   6  (bases 1 to 2966)
  AUTHORS   Stucke,V.M., Sillje,H.H., Arnaud,L. and Nigg,E.A.
  TITLE     Human Mps1 kinase is required for the spindle assembly checkpoint
            but not for centrosome duplication
  JOURNAL   EMBO J 21 (7), 1723-1732 (2002)
   PUBMED   11927556
  REMARK    GeneRIF: human Mps1(TTK kinase) is essential for the establishment
            and/or maintenance of the spindle assembly checkpoint, but
            dispensable for centrosome duplication.
REFERENCE   7  (bases 1 to 2966)
  AUTHORS   Cahill,D.P., da Costa,L.T., Carson-Walter,E.B., Kinzler,K.W.,
            Vogelstein,B. and Lengauer,C.
  TITLE     Characterization of MAD2B and other mitotic spindle checkpoint
            genes
  JOURNAL   Genomics 58 (2), 181-187 (1999)
   PUBMED   10366450
REFERENCE   8  (bases 1 to 2966)
  AUTHORS   Lindberg,R.A., Fischer,W.H. and Hunter,T.
  TITLE     Characterization of a human protein threonine kinase isolated by
            screening an expression library with antibodies to phosphotyrosine
  JOURNAL   Oncogene 8 (2), 351-359 (1993)
   PUBMED   7678926
REFERENCE   9  (bases 1 to 2966)
  AUTHORS   Mills,G.B., Schmandt,R., McGill,M., Amendola,A., Hill,M.,
            Jacobs,K., May,C., Rodricks,A.M., Campbell,S. and Hogg,D.
  TITLE     Expression of TTK, a novel human protein kinase, is associated with
            cell proliferation
  JOURNAL   J Biol Chem 267 (22), 16000-16006 (1992)
   PUBMED   1639825
REFERENCE   10 (bases 1 to 2966)
  AUTHORS   Hanks,S.K. and Quinn,A.M.
  TITLE     Protein kinase catalytic domain sequence database: identification
            of conserved features of primary structure and classification of
            family members
  JOURNAL   Methods Enzymol 200, 38-62 (1991)
   PUBMED   1956325
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA853919.1, AK315696.1 and
            AU154042.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003318.4.
            
            Summary: This gene encodes a dual specificity protein kinase with
            the ability to phosphorylate tyrosine, serine and threonine.
            Associated with cell proliferation, this protein is essential for
            chromosome alignment at the centromere during mitosis and is
            required for centrosome duplication. It has been found to be a
            critical mitotic checkpoint protein for accurate segregation of
            chromosomes during mitosis. Tumorigenesis may occur when this
            protein fails to degrade and produces excess centrosomes resulting
            in aberrant mitotic spindles. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Nov 2009].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC000633.2, SRR5189661.103703.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369798.7/ ENSP00000358813.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                DA853919.1         45-83
            40-2641             AK315696.1         1-2602
            2642-2966           AU154042.1         1-325               c
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q14.1"
     gene            1..2966
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /note="TTK protein kinase"
                     /db_xref="GeneID:7272"
                     /db_xref="HGNC:HGNC:12401"
                     /db_xref="MIM:604092"
     exon            1..65
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            66..206
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..2641
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /EC_number="2.7.12.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cancer/testis antigen 96; monopolar spindle 1-like 1;
                     phosphotyrosine picked threonine kinase; dual specificity
                     protein kinase TTK; phosphotyrosine picked
                     threonine-protein kinase; monopolar spindle 1 kinase"
                     /codon_start=1
                     /product="dual specificity protein kinase TTK isoform 1"
                     /protein_id="NP_003309.2"
                     /db_xref="CCDS:CCDS4993.1"
                     /db_xref="GeneID:7272"
                     /db_xref="HGNC:HGNC:12401"
                     /db_xref="MIM:604092"
                     /translation="MESEDLSGRELTIDSIMNKVRDIKNKFKNEDLTDELSLNKISAD
                     TTDNSGTVNQIMMMANNPEDWLSLLLKLEKNSVPLSDALLNKLIGRYSQAIEALPPDK
                     YGQNESFARIQVRFAELKAIQEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVK
                     KSKQLLQKAVERGAVPLEMLEIALRNLNLQKKQLLSEEEKKNLSASTVLTAQESFSGS
                     LGHLQNRNNSCDSRGQTTKARFLYGENMPPQDAEIGYRNSLRQTNKTKQSCPFGRVPV
                     NLLNSPDCDVKTDDSVVPCFMKRQTSRSECRDLVVPGSKPSGNDSCELRNLKSVQNSH
                     FKEPLVSDEKSSELIITDSITLKNKTESSLLAKLEETKEYQEPEVPESNQKQWQSKRK
                     SECINQNPAASSNHWQIPELARKVNTEQKHTTFEQPVFSVSKQSPPISTSKWFDPKSI
                     CKTPSSNTLDDYMSCFRTPVVKNDFPPACQLSTPYGQPACFQQQQHQILATPLQNLQV
                     LASSSANECISVKGRIYSILKQIGSGGSSKVFQVLNEKKQIYAIKYVNLEEADNQTLD
                     SYRNEIAYLNKLQQHSDKIIRLYDYEITDQYIYMVMECGNIDLNSWLKKKKSIDPWER
                     KSYWKNMLEAVHTIHQHGIVHSDLKPANFLIVDGMLKLIDFGIANQMQPDTTSVVKDS
                     QVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTYGKTPFQQIINQ
                     ISKLHAIIDPNHEIEFPDIPEKDLQDVLKCCLKRDPKQRISIPELLAHPYVQIQTHPV
                     NQMAKGTTEEMKYVLGQLVGLNSPNSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGK
                     K"
     exon            207..429
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            430..536
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            537..680
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            681..795
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            796..868
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            869..963
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1051
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1175
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1324
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1461
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1588
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1681
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1839
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..1991
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2116
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2197
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            2198..2374
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            2375..2459
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..2557
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     exon            2558..2966
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2932..2937
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /note="hexamer: AATAAA"
     polyA_site      2966
                     /gene="TTK"
                     /gene_synonym="CT96; ESK; MPH1; MPS1; MPS1L1; PYT"
                     /note="major polyA site"
ORIGIN      
        1 caaacgtgtt tgcggaaagg agtttgggtt ccatcttttc atttccccag cgcagctttc
       61 tgtagaaatg gaatccgagg atttaagtgg cagagaattg acaattgatt ccataatgaa
      121 caaagtgaga gacattaaaa ataagtttaa aaatgaagac cttactgatg aactaagctt
      181 gaataaaatt tctgctgata ctacagataa ctcgggaact gttaaccaaa ttatgatgat
      241 ggcaaacaac ccagaggact ggttgagttt gttgctcaaa ctagagaaaa acagtgttcc
      301 gctaagtgat gctcttttaa ataaattgat tggtcgttac agtcaagcaa ttgaagcgct
      361 tcccccagat aaatatggcc aaaatgagag ttttgctaga attcaagtga gatttgctga
      421 attaaaagct attcaagagc cagatgatgc acgtgactac tttcaaatgg ccagagcaaa
      481 ctgcaagaaa tttgcttttg ttcatatatc ttttgcacaa tttgaactgt cacaaggtaa
      541 tgtcaaaaaa agtaaacaac ttcttcaaaa agctgtagaa cgtggagcag taccactaga
      601 aatgctggaa attgccctgc ggaatttaaa cctccaaaaa aagcagctgc tttcagagga
      661 ggaaaagaag aatttatcag catctacggt attaactgcc caagaatcat tttccggttc
      721 acttgggcat ttacagaata ggaacaacag ttgtgattcc agaggacaga ctactaaagc
      781 caggttttta tatggagaga acatgccacc acaagatgca gaaataggtt accggaattc
      841 attgagacaa actaacaaaa ctaaacagtc atgcccattt ggaagagtcc cagttaacct
      901 tctaaatagc ccagattgtg atgtgaagac agatgattca gttgtacctt gttttatgaa
      961 aagacaaacc tctagatcag aatgccgaga tttggttgtg cctggatcta aaccaagtgg
     1021 aaatgattcc tgtgaattaa gaaatttaaa gtctgttcaa aatagtcatt tcaaggaacc
     1081 tctggtgtca gatgaaaaga gttctgaact tattattact gattcaataa ccctgaagaa
     1141 taaaacggaa tcaagtcttc tagctaaatt agaagaaact aaagagtatc aagaaccaga
     1201 ggttccagag agtaaccaga aacagtggca atctaagaga aagtcagagt gtattaacca
     1261 gaatcctgct gcatcttcaa atcactggca gattccggag ttagcccgaa aagttaatac
     1321 agagcagaaa cataccactt ttgagcaacc tgtcttttca gtttcaaaac agtcaccacc
     1381 aatatcaaca tctaaatggt ttgacccaaa atctatttgt aagacaccaa gcagcaatac
     1441 cttggatgat tacatgagct gttttagaac tccagttgta aagaatgact ttccacctgc
     1501 ttgtcagttg tcaacacctt atggccaacc tgcctgtttc cagcagcaac agcatcaaat
     1561 acttgccact ccacttcaaa atttacaggt tttagcatct tcttcagcaa atgaatgcat
     1621 ttcggttaaa ggaagaattt attccatatt aaagcagata ggaagtggag gttcaagcaa
     1681 ggtatttcag gtgttaaatg aaaagaaaca gatatatgct ataaaatatg tgaacttaga
     1741 agaagcagat aaccaaactc ttgatagtta ccggaacgaa atagcttatt tgaataaact
     1801 acaacaacac agtgataaga tcatccgact ttatgattat gaaatcacgg accagtacat
     1861 ctacatggta atggagtgtg gaaatattga tcttaatagt tggcttaaaa agaaaaaatc
     1921 cattgatcca tgggaacgca agagttactg gaaaaatatg ttagaggcag ttcacacaat
     1981 ccatcaacat ggcattgttc acagtgatct taaaccagct aactttctga tagttgatgg
     2041 aatgctaaag ctaattgatt ttgggattgc aaaccaaatg caaccagata caacaagtgt
     2101 tgttaaagat tctcaggttg gcacagttaa ttatatgcca ccagaagcaa tcaaagatat
     2161 gtcttcctcc agagagaatg ggaaatctaa gtcaaagata agccccaaaa gtgatgtttg
     2221 gtccttagga tgtattttgt actatatgac ttacgggaaa acaccatttc agcagataat
     2281 taatcagatt tctaaattac atgccataat tgatcctaat catgaaattg aatttcccga
     2341 tattccagag aaagatcttc aagatgtgtt aaagtgttgt ttaaaaaggg acccaaaaca
     2401 gaggatatcc attcctgagc tcctggctca tccatatgtt caaattcaaa ctcatccagt
     2461 taaccaaatg gccaagggaa ccactgaaga aatgaaatat gttctgggcc aacttgttgg
     2521 tctgaattct cctaactcca ttttgaaagc tgctaaaact ttatatgaac actatagtgg
     2581 tggtgaaagt cataattctt catcctccaa gacttttgaa aaaaaaaggg gaaaaaaatg
     2641 atttgcagtt attcgtaatg tcagatacca cctataaaat atattggact gttatactct
     2701 tgaatccctg tggaaatcta catttgaaga caacatcact ctgaagtgtt atcagcaaaa
     2761 aaaattcagt agattatctt taaaagaaaa ctgtaaaaat agcaaccact tatggcactg
     2821 tatatattgt agacttgttt tctctgtttt atgctcttgt gtaatctact tgacatcatt
     2881 ttactcttgg aatagtgggt ggatagcaag tatattctaa aaaactttgt aaataaagtt
     2941 ttgtggctaa aatgacacta acattt
//
