LOCUS       NP_003585               1162 aa            linear   PRI 20-SEP-2024
DEFINITION  transcription termination factor 2 [Homo sapiens].
ACCESSION   NP_003585
VERSION     NP_003585.3
DBSOURCE    REFSEQ: accession NM_003594.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 1162)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (residues 1 to 1162)
  AUTHORS   Miele,A., Medina,R., van Wijnen,A.J., Stein,G.S. and Stein,J.L.
  TITLE     The interactome of the histone gene regulatory factor HiNF-P
            suggests novel cell cycle related roles in transcriptional control
            and RNA processing
  JOURNAL   J Cell Biochem 102 (1), 136-148 (2007)
   PUBMED   17577209
REFERENCE   3  (residues 1 to 1162)
  AUTHORS   Jiang,Y. and Price,D.H.
  TITLE     Rescue of the TTF2 knockdown phenotype with an siRNA-resistant
            replacement vector
  JOURNAL   Cell Cycle 3 (9), 1151-1153 (2004)
   PUBMED   15467445
  REMARK    GeneRIF: Importantly, the mitotic phenotype seen with TTF2-siRNA is
            rescued by expression of the siRNA-resistant GFP-tagged TTF2
            proving that reduced TTF2 is responsible for the retention of RNA
            polymerase II on mitotic chromosomes.
REFERENCE   4  (residues 1 to 1162)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   5  (residues 1 to 1162)
  AUTHORS   Jiang,Y., Liu,M., Spencer,C.A. and Price,D.H.
  TITLE     Involvement of transcription termination factor 2 in mitotic
            repression of transcription elongation
  JOURNAL   Mol Cell 14 (3), 375-385 (2004)
   PUBMED   15125840
  REMARK    GeneRIF: TTF2 is the only ATP-dependent termination activity
            associated with Pol II transcription elongation complexes, is
            largely unaffected by template position, and is impervious to the
            phosphorylation state of the polymerase.
REFERENCE   6  (residues 1 to 1162)
  AUTHORS   Leonard,D., Ajuh,P., Lamond,A.I. and Legerski,R.J.
  TITLE     hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA
            splicing
  JOURNAL   Biochem Biophys Res Commun 308 (4), 793-801 (2003)
   PUBMED   12927788
  REMARK    GeneRIF: hLodestar/HuF2 interacts with CDC5L and is involved in
            pre-mRNA splicing
REFERENCE   7  (residues 1 to 1162)
  AUTHORS   Hara,R., Selby,C.P., Liu,M., Price,D.H. and Sancar,A.
  TITLE     Human transcription release factor 2 dissociates RNA polymerases I
            and II stalled at a cyclobutane thymine dimer
  JOURNAL   J Biol Chem 274 (35), 24779-24786 (1999)
   PUBMED   10455150
REFERENCE   8  (residues 1 to 1162)
  AUTHORS   Liu,M., Xie,Z. and Price,D.H.
  TITLE     A human RNA polymerase II transcription termination factor is a
            SWI2/SNF2 family member
  JOURNAL   J Biol Chem 273 (40), 25541-25544 (1998)
   PUBMED   9748214
REFERENCE   9  (residues 1 to 1162)
  AUTHORS   Girdham,C.H. and Glover,D.M.
  TITLE     Chromosome tangling and breakage at anaphase result from mutations
            in lodestar, a Drosophila gene encoding a putative nucleoside
            triphosphate-binding protein
  JOURNAL   Genes Dev 5 (10), 1786-1799 (1991)
   PUBMED   1916263
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL445231.8, AF073771.1,
            BC030058.1, AF080255.1, BQ422564.1, BI857056.1, BU182600.1,
            AW027795.1, H81916.1, AI991355.1 and AL391476.20.
            
            On Jan 13, 2004 this sequence version replaced NP_003585.2.
            
            Summary: This gene encodes a member of the SWI2/SNF2 family of
            proteins, which play a critical role in altering protein-DNA
            interactions. The encoded protein has been shown to have
            dsDNA-dependent ATPase activity and RNA polymerase II termination
            activity. This protein interacts with cell division cycle 5-like,
            associates with human splicing complexes, and plays a role in
            pre-mRNA splicing. [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.123353.1, AF080255.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369466.9/ ENSP00000358478.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p13.1"
     Protein         1..1162
                     /product="transcription termination factor 2"
                     /note="lodestar protein; human factor 2; lodestar homolog;
                     transcription release factor 2; RNA polymerase II
                     termination factor; zinc finger, GRF-type containing 6;
                     transcription termination factor, RNA polymerase II"
                     /calculated_mol_wt=129458
     Region          4..39
                     /region_name="zf-GRF"
                     /note="GRF zinc finger; cl46396"
                     /db_xref="CDD:480736"
     Region          97..116
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UNY4.2)"
     Region          142..358
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UNY4.2)"
     Region          <147..378
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          388..407
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UNY4.2)"
     Region          459..503
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UNY4.2)"
     Site            460
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:20068231; propagated from
                     UniProtKB/Swiss-Prot (Q9UNY4.2)"
     Region          <559..1142
                     /region_name="HepA"
                     /note="Superfamily II DNA or RNA helicase, SNF2 family
                     [Transcription, Replication, recombination, and repair];
                     COG0553"
                     /db_xref="CDD:440319"
     Region          570..820
                     /region_name="DEXHc_TTF2"
                     /note="DEAH-box helicase domain of TTF2; cd18072"
                     /db_xref="CDD:350830"
     Site            order(598..604,659,737..738)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350830"
     Region          737..740
                     /region_name="DEAH box"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UNY4.2)"
     Region          871..890
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UNY4.2)"
     Site            883
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9UNY4.2)"
     Site            908
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:20068231; propagated from
                     UniProtKB/Swiss-Prot (Q9UNY4.2)"
     CDS             1..1162
                     /gene="TTF2"
                     /gene_synonym="F2; HuF2; ZGRF6"
                     /coded_by="NM_003594.4:22..3510"
                     /db_xref="CCDS:CCDS892.1"
                     /db_xref="GeneID:8458"
                     /db_xref="HGNC:HGNC:12398"
                     /db_xref="MIM:604718"
ORIGIN      
        1 meevrcpehg tfcflktgvr dgpnkgksfy vcradtcsfv ratdipvshc llhedfvvel
       61 qglllpqdkk eyrlffrcir skaegkrwcg sipwqdpdsk ehsvsnksqh asetfhhssn
      121 wlrnpfkvld knqepalwkq likgegeekk adkkqrekgd qlfdqkkeqk pemmekdlss
      181 glvpkkkqsv vqekkqeega eiqceaetgg thkrdfseik sqqcqgnelt rpsassqeks
      241 sgksqdvqre seplrekvtq llpqnvhshn siskpqkggp lnkeytnwea ketkakdgps
      301 iqatqkslpq ghfqerpeth svpapggpaa qaapaapgls lgegreaats sddeeeddvv
      361 fvsskpgspl lfdstldlet kenlqfpdrs vqrkvspasg vskkvepsdp varrvylttq
      421 lkqkkstlas vniqalpdkg qklikqiqel eevlsgltls peqgtneksn sqvpqqshft
      481 ktttgpphlv ppqplprrgt qpvgslelks acqvtaggss qcyrghtnqd hvhavwkits
      541 eaigqlhrsl escpgetvva edpaglkvpl llhqkqalaw llwresqkpq ggiladdmgl
      601 gktltmiali ltqknqekke ekekstaltw lskddscdft shgtliicpa slihhwknev
      661 ekrvnsnklr vylyhgpnrd srarvlstyd ivittyslva keiptnkqea eipganlnve
      721 gtstpllria wariildeah nvknprvqts iavcklqaca rwavtgtpiq nnlldmysll
      781 kflrcspfde fnlwrsqvdn gskkggerls iltkslllrr tkdqldstgr plvilpqrkf
      841 qlhhlklsed eetvynvffa rsrsalqsyl krhesrgnqs grspnnpfsr valefgseep
      901 rhseaadspr sstvhilsql lrlrqccchl sllksaldpm elkgeglvls leeqlsaltl
      961 selrdsepss tvslngtffk melfegmres tkissllael eaiqrnsasq ksvivsqwtn
     1021 mlkvvalhlk khgltyatid gsvnpkqrmd lveafnhsrg pqvmlislla ggvglnltgg
     1081 nhlflldmhw npsledqacd riyrvgqqkd vvihrfvceg tveekilqlq ekkkdlakqv
     1141 lsgsgesvtk ltladlrvlf gi
//
