LOCUS       NM_024753               5415 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens tetratricopeptide repeat domain 21B (TTC21B), mRNA.
ACCESSION   NM_024753
VERSION     NM_024753.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Li,Y., Dai,L., Xu,H., Huang,J., Zhang,J., Mei,Z. and Zhang,R.
  TITLE     Clinical report and genetic analysis of rare premature infant
            nephronophthisis caused by biallelic TTC21B variants
  JOURNAL   Mol Genet Genomic Med 12 (3), e2399 (2024)
   PUBMED   38439578
  REMARK    GeneRIF: Clinical report and genetic analysis of rare premature
            infant nephronophthisis caused by biallelic TTC21B variants.
            Review article
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Viehl,L., Wegner,D.J., Hmiel,S.P., White,F.V., Jain,S., Cole,F.S.
            and Wambach,J.A.
  TITLE     Lethal neonatal respiratory failure due to biallelic variants in
            BBS1 and monoallelic variant in TTC21B
  JOURNAL   Pediatr Nephrol 38 (2), 605-609 (2023)
   PUBMED   35695966
  REMARK    GeneRIF: Lethal neonatal respiratory failure due to biallelic
            variants in BBS1 and monoallelic variant in TTC21B.
REFERENCE   3  (bases 1 to 5415)
  AUTHORS   Chen,W., Wang,F., Zeng,W., Zhang,X., Shen,L., Zhang,Y. and Zhou,X.
  TITLE     Biallelic mutations of TTC12 and TTC21B were identified in Chinese
            patients with multisystem ciliopathy syndromes
  JOURNAL   Hum Genomics 16 (1), 48 (2022)
   PUBMED   36273201
  REMARK    GeneRIF: Biallelic mutations of TTC12 and TTC21B were identified in
            Chinese patients with multisystem ciliopathy syndromes.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5415)
  AUTHORS   Ben-Yosef,T., Asia Batsir,N., Ali Nasser,T. and Ehrenberg,M.
  TITLE     Retinal dystrophy as part of TTC21B-associated ciliopathy
  JOURNAL   Ophthalmic Genet 42 (3), 329-333 (2021)
   PUBMED   33599192
  REMARK    GeneRIF: Retinal dystrophy as part of TTC21B-associated ciliopathy.
REFERENCE   5  (bases 1 to 5415)
  AUTHORS   Otto,E.A., Ramaswami,G., Janssen,S., Chaki,M., Allen,S.J., Zhou,W.,
            Airik,R., Hurd,T.W., Ghosh,A.K., Wolf,M.T., Hoppe,B., Neuhaus,T.J.,
            Bockenhauer,D., Milford,D.V., Soliman,N.A., Antignac,C.,
            Saunier,S., Johnson,C.A. and Hildebrandt,F.
  CONSRTM   GPN Study Group
  TITLE     Mutation analysis of 18 nephronophthisis associated ciliopathy
            disease genes using a DNA pooling and next generation sequencing
            strategy
  JOURNAL   J Med Genet 48 (2), 105-116 (2011)
   PUBMED   21068128
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[J Med Genet. 2015 Dec;52(12):866. doi:
            10.1136/jmg-2010-082552corr1.. PMID: 26603841]
REFERENCE   6  (bases 1 to 5415)
  AUTHORS   Mukhopadhyay,S., Wen,X., Chih,B., Nelson,C.D., Lane,W.S.,
            Scales,S.J. and Jackson,P.K.
  TITLE     TULP3 bridges the IFT-A complex and membrane phosphoinositides to
            promote trafficking of G protein-coupled receptors into primary
            cilia
  JOURNAL   Genes Dev 24 (19), 2180-2193 (2010)
   PUBMED   20889716
REFERENCE   7  (bases 1 to 5415)
  AUTHORS   Ohara,O., Nagase,T., Mitsui,G., Kohga,H., Kikuno,R., Hiraoka,S.,
            Takahashi,Y., Kitajima,S., Saga,Y. and Koseki,H.
  TITLE     Characterization of size-fractionated cDNA libraries generated by
            the in vitro recombination-assisted method
  JOURNAL   DNA Res 9 (2), 47-57 (2002)
   PUBMED   12056414
REFERENCE   8  (bases 1 to 5415)
  AUTHORS   Stokman,M., Lilien,M. and Knoers,N.
  TITLE     Nephronophthisis-Related Ciliopathies
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   27336129
REFERENCE   9  (bases 1 to 5415)
  AUTHORS   Gunay-Aygun,M., Gahl,W.A. and Heller,T.
  TITLE     Congenital Hepatic Fibrosis Overview horizontal line RETIRED
            CHAPTER, FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301743
REFERENCE   10 (bases 1 to 5415)
  AUTHORS   Parisi,M. and Glass,I.
  TITLE     Joubert Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301500
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC055424.1, AK292942.1,
            AC011241.7, BC035767.2, CK905149.1 and BX114229.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_024753.4.
            
            Summary: This gene encodes a member of TTC21 family, containing
            several tetratricopeptide repeat (TPR) domains. This protein is
            localized to the cilium axoneme, and may play a role in retrograde
            intraflagellar transport in cilia. Mutations in this gene are
            associated with various ciliopathies, nephronophthisis 12, and
            asphyxiating thoracic dystrophy 4. [provided by RefSeq, Oct 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.3660294.1, AK292942.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000243344.8/ ENSP00000243344.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-671               BC055424.1         63-733
            672-794             AK292942.1         677-799
            795-896             BC055424.1         857-958
            897-897             AC011241.7         91180-91180         c
            898-4438            BC055424.1         960-4500
            4439-4834           BC035767.2         1267-1662
            4835-5398           CK905149.1         1-564               c
            5399-5415           BX114229.1         354-370
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.3"
     gene            1..5415
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /note="tetratricopeptide repeat domain 21B"
                     /db_xref="GeneID:79809"
                     /db_xref="HGNC:HGNC:25660"
                     /db_xref="MIM:612014"
     exon            1..92
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..4022
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /note="putative protein product of Nbla10696; TPR repeat
                     protein 21B; intraflagellar transport 139 homolog;
                     tetratricopeptide repeat-containing hedgehog modulator-1;
                     Nephronophthisis type12"
                     /codon_start=1
                     /product="tetratricopeptide repeat protein 21B"
                     /protein_id="NP_079029.3"
                     /db_xref="CCDS:CCDS33315.1"
                     /db_xref="GeneID:79809"
                     /db_xref="HGNC:HGNC:25660"
                     /db_xref="MIM:612014"
                     /translation="MDSQELKTLINYYCQERYFHHVLLVASEGIKRYGSDPVFRFYHA
                     YGTLMEGKTQEALREFEAIKNKQDVSLCSLLALIYAHKMSPNPDREAILESDARVKEQ
                     RKGAGEKALYHAGLFLWHIGRHDKAREYIDRMIKISDGSKQGHVLKAWLDITRGKEPY
                     TKKALKYFEEGLQDGNDTFALLGKAQCLEMRQNYSGALETVNQIIVNFPSFLPAFVKK
                     MKLQLALQDWDQTVETAQRLLLQDSQNVEALRMQALYYVCREGDIEKASTKLENLGNT
                     LDAMEPQNAQLFYNITLAFSRTCGRSQLILQKIQTLLERAFSLNPQQSEFATELGYQM
                     ILQGRVKEALKWYKTAMTLDETSVSALVGFIQCQLIEGQLQDADQQLEFLNEIQQSIG
                     KSAELIYLHAVLAMKKNKRQEEVINLLNDVLDTHFSQLEGLPLGIQYFEKLNPDFLLE
                     IVMEYLSFCPMQPASPGQPLCPLLRRCISVLETVVRTVPGLLQTVFLIAKVKYLSGDI
                     EAAFNNLQHCLEHNPSYADAHLLLAQVYLSQEKVKLCSQSLELCLSYDFKVRDYPLYH
                     LIKAQSQKKMGEIADAIKTLHMAMSLPGMKRIGASTKSKDRKTEVDTSHRLSIFLELI
                     DVHRLNGEQHEATKVLQDAIHEFSGTSEEVRVTIANADLALAQGDIERALSILQNVTA
                     EQPYFIEAREKMADIYLKHRKDKMLYITCFREIAERMANPRSFLLLGDAYMNILEPEE
                     AIVAYEQALNQNPKDGTLASKMGKALIKTHNYSMAITYYEAALKTGQKNYLCYDLAEL
                     LLKLKWYDKAEKVLQHALAHEPVNELSALMEDGRCQVLLAKVYSKMEKLGDAITALQQ
                     ARELQARVLKRVQMEQPDAVPAQKHLAAEICAEIAKHSVAQRDYEKAIKFYREALVHC
                     ETDNKIMLELARLYLAQDDPDSCLRQCALLLQSDQDNEAATMMMADLMFRKQDYEQAV
                     FHLQQLLERKPDNYMTLSRLIDLLRRCGKLEDVPRFFSMAEKRNSRAKLEPGFQYCKG
                     LYLWYTGEPNDALRHFNKARKDRDWGQNALYNMIEICLNPDNETVGGEVFENLDGDLG
                     NSTEKQESVQLAVRTAEKLLKELKPQTVQGHVQLRIMENYCLMATKQKSNVEQALNTF
                     TEIAASEKEHIPALLGMATAYMILKQTPRARNQLKRIAKMNWNAIDAEEFEKSWLLLA
                     DIYIQSAKYDMAEDLLKRCLRHNRSCCKAYEYMGYIMEKEQAYTDAALNYEMAWKYSN
                     RTNPAVGYKLAFNYLKAKRYVDSIDICHQVLEAHPTYPKIRKDILDKARASLRP"
     exon            93..222
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            223..333
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            334..500
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            501..623
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            624..781
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            782..866
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            867..965
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1158
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1159..1256
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1457
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1587
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1745
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1746..1970
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1971..2209
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..2282
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2283..2393
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2532
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2639
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2640..2828
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2829..2939
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2940..3021
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3022..3172
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3173..3334
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3335..3530
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3531..3755
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3756..3876
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3877..3944
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3945..5415
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5394..5399
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /note="hexamer: AATAAA"
     polyA_site      5415
                     /gene="TTC21B"
                     /gene_synonym="ATD4; CFAP60; FAP60; FLA17; IFT139;
                     IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1"
                     /note="major polyA site"
ORIGIN      
        1 gaattctgcg tcttccttta ggctgcggcg agacaatccc cagagccccg cggcccggcc
       61 tcggggcagc catggactcg caggaattga agactttgat taattactat tgtcaagaga
      121 gatatttcca tcatgtatta ctggttgcca gtgaaggaat taagaggtat ggaagtgatc
      181 cagtcttcag gttttatcat gcctatggca cattaatgga aggtaaaact caagaagctc
      241 ttcgagaatt tgaggctatt aaaaataaac aagatgtatc actttgttct ctacttgcac
      301 tgatatatgc ccataaaatg agtcctaatc cagatagaga agctattctg gaatcagatg
      361 ccagagtgaa ggaacaacgt aaaggagctg gagagaaagc cttataccat gcaggcttat
      421 ttttatggca cattggtcgc catgataaag caagggaata tattgacaga atgatcaaaa
      481 tatcagatgg tagtaaacag ggacacgttt tgaaagcatg gcttgatatt acaagaggaa
      541 aagagcctta cactaaaaaa gcactgaagt attttgaaga gggactccaa gatgggaatg
      601 atacttttgc tctgctgggt aaggcacaat gccttgagat gcgccagaat tattcaggtg
      661 ccctggagac tgtgaaccag ataatcgtga attttccgag cttccttcct gcttttgtta
      721 agaaaatgaa attacaacta gccttgcagg attgggacca gacagttgag acagcacaaa
      781 ggttgctgct ccaagatagc caaaatgtgg aagcactgag aatgcaggca ctctactatg
      841 tgtgtagaga gggggatata gagaaggctt ccaccaagct ggaaaacttg ggaaatacat
      901 tggatgccat ggaaccacag aatgctcaac ttttctataa cattacactc gccttcagca
      961 gaacttgtgg acgtagtcaa cttattcttc aaaaaattca aacgttactt gagagagctt
     1021 ttagtttaaa ccctcagcaa tcagaatttg ctacagaact tggataccaa atgattttac
     1081 aaggaagagt taaagaggca ctgaagtggt ataagaccgc catgacactt gatgagacta
     1141 gtgtgtctgc cctagttgga tttatccaat gtcagttgat agaagggcaa ttacaggatg
     1201 cagatcagca gctagaattt ttaaatgaaa tccagcaatc cattggaaaa tctgcggaat
     1261 taatctattt acatgcagtt cttgccatga agaaaaataa acgacaagaa gaagttatta
     1321 atttgttaaa tgatgtcctg gacactcact tttcacaatt agaaggtttg cctcttggca
     1381 tacagtattt tgaaaagcta aatcctgatt tcttgttaga aattgttatg gagtatctga
     1441 gcttctgtcc aatgcagcct gcaagtcctg ggcaacctct ttgtccactt ctcaggcgtt
     1501 gcatctcagt cctggagact gtagtaagaa ctgttccagg tcttctgcaa acagtcttcc
     1561 taatagcaaa agtgaaatat ttgtcaggtg atattgaagc agctttcaat aaccttcagc
     1621 actgcttaga acacaatccc tcttatgctg atgctcatct gctgctagct caggtttact
     1681 tgtctcaaga aaaagtcaaa ttgtgttctc agtctcttga actttgtctg agctatgatt
     1741 ttaaggtgag agactatcct ttataccatt tgataaaagc tcagtcacaa aagaaaatgg
     1801 gagaaatagc agacgcaatt aaaacactgc atatggcaat gagtttacca ggaatgaaaa
     1861 gaattggagc ttccacaaaa tcaaaagaca gaaaaactga agttgataca agccatcgtt
     1921 tatcgatctt tcttgaattg atagacgttc accgcttaaa tggagagcag catgaggcaa
     1981 ccaaagtttt acaagatgcc atccatgaat tttctggaac atctgaagaa gtgcgggtta
     2041 ccattgctaa tgcagacctt gctctagccc aaggagatat tgaacgggct ttaagcatcc
     2101 ttcagaatgt tacagccgaa cagccttatt ttatagaggc cagagaaaaa atggcagata
     2161 tttatctgaa gcacagaaaa gataaaatgt tatatatcac ttgttttaga gaaattgctg
     2221 aaagaatggc taaccctcgg tcttttcttc tccttggtga tgcatacatg aatattctag
     2281 agcctgaaga agccatagta gcatatgagc aagcattaaa tcagaacccg aaagatggaa
     2341 cattggcaag caaaatgggc aaagcactta tcaaaactca taactactca atggcaatca
     2401 cttactatga agctgctctg aaaactggac aaaagaatta tctttgctat gacctggctg
     2461 agctcttatt aaaattgaaa tggtatgaca aagcagaaaa agttcttcag catgctctgg
     2521 ctcatgaacc tgtaaatgaa ctgtcagctc tcatggagga tggacgttgt caagttcttc
     2581 tagcaaaagt ttatagtaaa atggaaaaac ttggtgatgc gatcactgca ttacaacagg
     2641 ctcgagaatt acaagctcgg gtactaaaac gtgttcagat ggaacagcca gatgcagttc
     2701 ctgcacagaa acatttagca gctgaaattt gtgcagagat tgcaaaacat tctgttgctc
     2761 agcgagacta tgaaaaagca attaagtttt atagagaggc tctggttcac tgcgaaacag
     2821 ataataagat tatgttggaa ctggcacgat tatacctggc acaagatgac cctgattcct
     2881 gcctgcggca gtgtgctcta ctgcttcaga gtgaccagga taacgaagct gctaccatga
     2941 tgatggctga tctcatgttc agaaaacaag actatgaaca agcagtgttt catttacagc
     3001 agcttttaga acgtaagcca gataattata tgacattatc tcgtttgatt gatctcctaa
     3061 gaagatgtgg aaaactcgag gatgtcccaa gatttttctc aatggctgag aaacgtaact
     3121 ccagagcaaa attggaacca ggatttcagt attgtaaagg actgtatctt tggtacactg
     3181 gagaaccaaa tgatgccctt cgacatttta ataaagctcg gaaagatcgt gactggggcc
     3241 aaaatgccct ttataatatg atagagatct gtttgaatcc agataatgaa actgttggag
     3301 gtgaagtatt tgaaaacctg gatggagacc tgggtaattc aactgagaag caagaatctg
     3361 tgcaactggc agtaagaaca gcagaaaaac ttcttaagga actaaaacct cagactgttc
     3421 agggtcacgt acagcttcgc ataatggaaa actattgctt aatggctacc aaacagaaat
     3481 ctaatgttga acaagcatta aataccttca ctgaaatagc agcatctgag aaggagcata
     3541 tcccagcgct cttgggaatg gcaacggctt atatgatctt gaaacagact ccacgagcca
     3601 gaaaccagct gaagcgtatt gcgaaaatga attggaatgc tattgatgct gaagagtttg
     3661 agaagagttg gctgctactt gctgatattt acattcaatc agcaaaatat gacatggcag
     3721 aagacctgtt aaaacggtgc ctgcgtcata atagatcttg ctgcaaagct tatgaatata
     3781 tgggatacat tatggaaaaa gagcaagcat atacagatgc tgccttgaac tatgagatgg
     3841 catggaaata tagcaatcgg acaaatccgg cagtaggata caaactggca tttaattact
     3901 taaaagcaaa aagatatgtg gattcaattg acatatgtca ccaggttctt gaagcacatc
     3961 caacttatcc aaaaatcaga aaggatatac ttgataaggc ccgtgcgtct ttaagacctt
     4021 gaaaataatt ttaacttagg tgttggttta acaggaaatg aaagaaatct aactttcagt
     4081 tcttcctgtt caaaacaggt ttgagctcag tgctttgtta ttagaagtat accctttttt
     4141 ctccagcaga ggttgctgct gtacatcaag agaagtacta tgtgaaattg gtgtttccta
     4201 atggagttga atgagagctg gtctatttga ctctgttttg attgggtaca gatttggtga
     4261 ctctgtggta aagactataa ttatttctat aaagaatatt ttgttaaaat ctaggtaatt
     4321 aaataccctg tatcttttct aaggaatatt atttcaggaa atatatttaa aatgcattgt
     4381 tctcttttaa agtgtttttg ttatattctt ttatttattt atttattttt aaagacagag
     4441 ccttgatctg ccgcccaggc tggagtgcag tggcacgatc ttggctcact gcaacctccg
     4501 cctcctgggt ttacgcgatt ctcctgcttc agcctcccga gtagctggga ctaaaggcat
     4561 gtgccatcac acccggctaa ttttttgtat ttttagtaga gatgggattt caccgtgtta
     4621 gccaggaggg gcttgatctc ttgaccttgt gatccacctg cctcggcctc ccaaagtgct
     4681 gggattacag gcatgagcca ccgcacccag cctgtttttg ttgtattctg tgttgtttca
     4741 aaaattggaa taaatctccc tgtaaataac tcatgaaaaa gacaatatat atattttaaa
     4801 tatatatttt aataaaggtt ttataaatag atgatttata ttcctcttgg aactcaaaag
     4861 atgtggtaag ttttgcaaat cagggtaagc tccccctgca ttctgtaatg ttaatgggtg
     4921 gtggtatagt acagaaaatg taactttcat tgaaaaatct tttttagaaa agggtagagt
     4981 gtgtgtttcc ctttcctttc ttgagctcat tcttcctgct ttcaaggcca ttgtggtctt
     5041 aagaaataat caggtcctga aacctcaatt taaataggtc ccttattagg gatatttaac
     5101 cttagcctag aagacttggt ctgcaggtat aatttcagac actgtaacat ttgatggtaa
     5161 agggtgacca aatacttttg gtcagcaggg agtcacaaag cacaccatgt gaaggcaacg
     5221 gtttacccag ctctgtttaa cagcaccctg gcatagaagc tttcactctc ttacactaaa
     5281 tttaacctaa tctaaactta atccttagca ttatttttaa tttctcatct acatcttttt
     5341 caattttcct taaaattgta gtaaacacag tcctcaattt tatttgcatt tacaataaac
     5401 agtatttata agtca
//
