LOCUS       NM_173500              11208 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens tau tubulin kinase 2 (TTBK2), mRNA.
ACCESSION   NM_173500
VERSION     NM_173500.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Lu,Y.Q., Chen,J.M., Huang,Y.L. and Zou,Z.Y.
  TITLE     A Novel TTBK2 Mutation in a Chinese Pedigree with Spinocerebellar
            Ataxia 11
  JOURNAL   Cerebellum 23 (3), 1221-1225 (2024)
   PUBMED   37848700
  REMARK    GeneRIF: A Novel TTBK2 Mutation in a Chinese Pedigree with
            Spinocerebellar Ataxia 11.
REFERENCE   2  (bases 1 to 11208)
  AUTHORS   Felicio,D. and Santos,M.
  TITLE     Spinocerebellar ataxia type 11 (SCA11): TTBK2 variants, functions
            and associated disease mechanisms
  JOURNAL   Cerebellum 23 (2), 678-687 (2024)
   PUBMED   36892783
  REMARK    GeneRIF: Spinocerebellar ataxia type 11 (SCA11): TTBK2 variants,
            functions and associated disease mechanisms.
            Review article
REFERENCE   3  (bases 1 to 11208)
  AUTHORS   Bashore,F.M., Marquez,A.B., Chaikuad,A., Howell,S., Dunn,A.S.,
            Beltran,A.A., Smith,J.L., Drewry,D.H., Beltran,A.S. and Axtman,A.D.
  TITLE     Modulation of tau tubulin kinases (TTBK1 and TTBK2) impacts
            ciliogenesis
  JOURNAL   Sci Rep 13 (1), 6118 (2023)
   PUBMED   37059819
  REMARK    GeneRIF: Modulation of tau tubulin kinases (TTBK1 and TTBK2)
            impacts ciliogenesis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11208)
  AUTHORS   Rosa E Silva,I., Bino,L., Johnson,C.M., Rutherford,T.J.,
            Neuhaus,D., Andreeva,A., Cajanek,L. and van Breugel,M.
  TITLE     Molecular mechanisms underlying the role of the centriolar
            CEP164-TTBK2 complex in ciliopathies
  JOURNAL   Structure 30 (1), 114-128 (2022)
   PUBMED   34499853
  REMARK    GeneRIF: Molecular mechanisms underlying the role of the centriolar
            CEP164-TTBK2 complex in ciliopathies.
REFERENCE   5  (bases 1 to 11208)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   6  (bases 1 to 11208)
  AUTHORS   Brusco,A., Gellera,C., Cagnoli,C., Saluto,A., Castucci,A.,
            Michielotto,C., Fetoni,V., Mariotti,C., Migone,N., Di Donato,S. and
            Taroni,F.
  TITLE     Molecular genetics of hereditary spinocerebellar ataxia: mutation
            analysis of spinocerebellar ataxia genes and CAG/CTG repeat
            expansion detection in 225 Italian families
  JOURNAL   Arch Neurol 61 (5), 727-733 (2004)
   PUBMED   15148151
  REMARK    GeneRIF: Observational study of genotype prevalence. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 11208)
  AUTHORS   Dgany,O., Avidan,N., Delaunay,J., Krasnov,T., Shalmon,L.,
            Shalev,H., Eidelitz-Markus,T., Kapelushnik,J., Cattan,D.,
            Pariente,A., Tulliez,M., Cretien,A., Schischmanoff,P.O.,
            Iolascon,A., Fibach,E., Koren,A., Rossler,J., Le Merrer,M.,
            Yaniv,I., Zaizov,R., Ben-Asher,E., Olender,T., Lancet,D.,
            Beckmann,J.S. and Tamary,H.
  TITLE     Congenital dyserythropoietic anemia type I is caused by mutations
            in codanin-1
  JOURNAL   Am J Hum Genet 71 (6), 1467-1474 (2002)
   PUBMED   12434312
REFERENCE   8  (bases 1 to 11208)
  AUTHORS   Worth,P.F., Giunti,P., Gardner-Thorpe,C., Dixon,P.H., Davis,M.B.
            and Wood,N.W.
  TITLE     Autosomal dominant cerebellar ataxia type III: linkage in a large
            British family to a 7.6-cM region on chromosome 15q14-21.3
  JOURNAL   Am J Hum Genet 65 (2), 420-426 (1999)
   PUBMED   10417284
REFERENCE   9  (bases 1 to 11208)
  AUTHORS   Chen,Z., Puzriakova,A. and Houlden,H.
  TITLE     Spinocerebellar Ataxia Type 11
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301723
REFERENCE   10 (bases 1 to 11208)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC041876.1, AF525400.1,
            BC071556.1, AB020654.1, CB242184.1 and AC090510.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_173500.3.
            
            Summary: This gene encodes a serine-threonine kinase that
            putatively phosphorylates tau and tubulin proteins. Mutations in
            this gene cause spinocerebellar ataxia type 11 (SCA11); a
            neurodegenerative disease characterized by progressive ataxia and
            atrophy of the cerebellum and brainstem. [provided by RefSeq, Aug
            2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.2278035.1,
                                           SRR1660803.41021.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000267890.11/ ENSP00000267890.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-64                BC041876.1         32-95
            65-430              AF525400.1         1-366
            431-1817            BC041876.1         462-1848
            1818-4254           BC071556.1         1623-4059
            4255-5590           AB020654.1         2051-3386
            5591-5600           CB242184.1         5-14                c
            5601-11208          AC090510.4         99748-105355        c
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.2"
     gene            1..11208
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /note="tau tubulin kinase 2"
                     /db_xref="GeneID:146057"
                     /db_xref="HGNC:HGNC:19141"
                     /db_xref="MIM:611695"
     exon            1..341
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            342..477
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     CDS             409..4143
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /EC_number="2.7.11.1"
                     /codon_start=1
                     /product="tau-tubulin kinase 2"
                     /protein_id="NP_775771.3"
                     /db_xref="CCDS:CCDS42029.1"
                     /db_xref="GeneID:146057"
                     /db_xref="HGNC:HGNC:19141"
                     /db_xref="MIM:611695"
                     /translation="MSGGGEQLDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTR
                     ENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLA
                     DLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNFAMGRFPSTCRKCY
                     MLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVE
                     FVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSIFLDHISSLDYFTKPDYQLL
                     TSVFDNSIKTFGVIESDPFDWEKTGNDGSLTTTTTSTTPQLHTRLTPAAIGIANATPI
                     PGDLLRENTDEVFPDEQLSDGENGIPVGVSPDKLPGSLGHPRPQEKDVWEEMDANKNK
                     IKLGICKAATEEENSHGQANGLLNAPSLGSPIRVRSEITQPDRDIPLVRKLRSIHSFE
                     LEKRLTLEPKPDTDKFLETCLEKMQKDTSAGKESILPALLHKPCVPAVSRTDHIWHYD
                     EEYLPDASKPASANTPEQADGGGSNGFIAVNLSSCKQEIDSKEWVIVDKEQDLQDFRT
                     NEAVGHKTTGSPSDEEPEVLQVLEASPQDEKLQLGPWAENDHLKKETSGVVLALSAEG
                     PPTAASEQYTDRLELQPGAASQFIAATPTSLMEAQAEGPLTAITIPRPSVASTQSTSG
                     SFHCGQQPEKKDLQPMEPTVELYSPRENFSGLVVTEGEPPSGGSRTDLGLQIDHIGHD
                     MLPNIRESNKSQDLGPKELPDHNRLVVREFENLPGETEEKSILLESDNEDEKLSRGQH
                     CIEISSLPGDLVIVEKDHSATTEPLDVTKTQTFSVVPNQDKNNEIMKLLTVGTSEISS
                     RDIDPHVEGQIGQVAEMQKNKISKDDDIMSEDLPGHQGDLSTFLHQEGKREKITPRNG
                     ELFHCVSENEHGAPTRKDMVRSSFVTRHSRIPVLAQEIDSTLESSSPVSAKEKLLQKK
                     AYQPDLVKLLVEKRQFKSFLGDLSSASDKLLEEKLATVPAPFCEEEVLTPFSRLTVDS
                     HLSRSAEDSFLSPIISQSRKSKIPRPVSWVNTDQVNSSTSSQFFPRPPPGKPPTRPGV
                     EARLRRYKVLGSSNSDSDLFSRLAQILQNGSQKPRSTTQCKSPGSPHNPKTPPKSPVV
                     PRRSPSASPRSSSLPRTSSSSPSRAGRPHHDQRSSSPHLGRSKSPPSHSGSSSSRRSC
                     QQEHCKPSKNGLKGSGSLHHHSASTKTPQGKSKPASKLSR"
     exon            478..625
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            626..699
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            700..840
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            841..945
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1011
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1104
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1230
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1388
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1605
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            1606..1817
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..2406
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            2407..3680
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     exon            3681..11208
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10938..10943
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /note="hexamer: AATAAA"
     polyA_site      10964
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /note="major polyA site"
     regulatory      11184..11189
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
                     /note="hexamer: AATAAA"
     polyA_site      11208
                     /gene="TTBK2"
                     /gene_synonym="SCA11; TTBK"
ORIGIN      
        1 gcagtagtcg ccgcagcggc gatgggagcg gtggggacga ggcggcggcg gcggcaggag
       61 ggggagcagg tgctggcaca agagcagcgg cttgggggag ccggcagcag cagtaacagc
      121 agcagcagcc gccgccgccg ccgccagtaa acgcggacgg taccccaggg gactacccag
      181 ccggccggcc ctggaagccg cgctcgggtc ccgccgcagt cggcggtggg ggatgggcag
      241 gcagtggcgg tcccgcctgc cgagggttaa cccccgccgg tcccggtcct gagctggacc
      301 agagccctcc tccagaaacc cctgcgtccg ccacggccca ggttaaatgg aaaccaccct
      361 tgggaactgg atgcctgtgt agctgttcta ccatatcagt gtattgcaat gagtggggga
      421 ggagagcagc tggatatcct gagtgttgga atcctagtga aagaaagatg gaaagtgttg
      481 agaaagattg ggggtggggg ctttggagaa atttacgatg ccttggacat gctcaccagg
      541 gaaaatgttg cactgaaggt ggaatcagct caacaaccaa aacaagttct gaaaatggaa
      601 gttgctgttt tgaaaaagct gcaagggaaa gaccatgttt gtagatttat tggctgtggg
      661 aggaatgatc gattcaacta tgtggtcatg cagttgcagg gtcggaatct ggcagatctt
      721 cgccgtagcc agtcccgagg cacattcacc attagtacca ctctccggct gggtagacag
      781 attttggagt ctattgaaag cattcattct gtgggattct tgcatcgaga catcaaaccg
      841 tcgaacttcg ctatgggtcg ctttcctagt acatgtagga aatgttacat gcttgatttt
      901 ggcttggctc gacaatttac caattcctgt ggtgacgtca gaccacctcg agctgtggca
      961 ggttttcgag ggacagttcg ttatgcatca atcaacgcac atcggaacag ggaaatggga
     1021 agacatgatg acctttggtc cttattctac atgttggtgg agtttgtggt tggtcagctg
     1081 ccctggagaa aaataaagga caaggagcaa gtaggctcta ttaaggagag atatgaccac
     1141 aggctcatgt tgaaacatct ccctccagaa ttcagcatct ttctagacca tatctcttct
     1201 ttggattatt ttacaaaacc agactaccag cttcttacat ccgtgtttga caatagcatc
     1261 aagacttttg gagtaattga gagtgaccct tttgactggg agaagactgg aaatgatggc
     1321 tccctaacaa ccaccactac ttctaccacc cctcagttgc acactcgctt gacccctgct
     1381 gcaattggaa ttgccaatgc tactcccatc cctggagact tgcttcgaga aaatacagat
     1441 gaggtatttc cagatgaaca gcttagcgat ggagaaaatg gcatccctgt tggtgtgtca
     1501 ccagataaat tgcctggatc tctgggacac ccccgtcccc aggagaagga tgtttgggaa
     1561 gagatggatg ccaacaaaaa caagataaag cttggaattt gtaaggctgc tactgaagag
     1621 gagaacagcc atggccaggc aaatggtctt ctcaatgctc caagccttgg gtcaccaatt
     1681 cgtgtccgct cagagattac tcagccagac agagatattc cactggtgcg aaagttacgt
     1741 tccattcaca gctttgagct ggaaaaacgt ctgaccctgg agccaaagcc agacactgac
     1801 aagttccttg agacctgcct ggagaaaatg cagaaagata ccagtgcagg aaaagaatct
     1861 attctccctg ctctgctgca taagccttgc gttcctgctg tgtcccgtac tgaccacatc
     1921 tggcactatg atgaagaata tcttccagat gcctccaagc ctgcttctgc caacacccct
     1981 gagcaggcag atggtggtgg cagcaatgga tttatagctg ttaacctgag ctcttgcaag
     2041 caagaaattg attccaaaga atgggtgatt gtggacaagg agcaggacct tcaggatttt
     2101 aggacaaatg aggctgtagg acataaaaca actggaagtc cttctgatga ggagcctgaa
     2161 gtacttcaag tcctggaggc atcacctcaa gatgaaaagc tccagttagg tccttgggca
     2221 gaaaatgatc atttaaagaa ggaaacctca ggtgtggtct tagcactttc tgcagagggt
     2281 cctcctactg ctgcttcaga acaatataca gataggctgg aactccagcc tggagctgct
     2341 agtcagttta ttgcagcgac gcccacaagt ctaatggagg cgcaggcaga aggacccctt
     2401 acagcgatta caattcctag accttctgtg gcatctacac agtcaacttc aggaagcttt
     2461 cactgtggtc agcagccaga gaagaaagat cttcagccca tggagcccac tgtggaactt
     2521 tactctccaa gggaaaactt ctctggcttg gttgtgacag agggtgaacc tcctagtgga
     2581 ggaagcagaa cagatttggg gcttcagata gatcacattg gtcatgacat gttacccaac
     2641 attagagaaa gtaacaaatc tcaagacctg ggaccaaaag aacttcctga tcataataga
     2701 ctggttgtga gagaatttga aaatctccct ggggaaactg aagagaaaag catcctttta
     2761 gagtcagata atgaagatga gaagttaagt agagggcagc attgtattga gatctcctct
     2821 ctcccaggag atttggtaat tgtggaaaag gatcactcag ctactactga acctcttgat
     2881 gtgacaaaaa cacagacttt tagtgtggtg ccaaatcaag acaaaaataa tgagataatg
     2941 aagcttctga cagttggaac ttcagaaatt tcttccagag acattgaccc acatgttgaa
     3001 ggtcagatag gccaagtggc agaaatgcaa aaaaataaga tatctaagga tgatgacatc
     3061 atgagtgaag acttgccagg tcatcaagga gacctctcta cttttttgca ccaagagggc
     3121 aagagagaga aaatcacccc tagaaatgga gaactatttc attgtgtttc agagaatgaa
     3181 catggtgccc caacccggaa ggatatggtt aggtcatcct ttgtaactag acacagccga
     3241 atccctgttt tagcacaaga gatagactca actttggaat catcctctcc agtttctgca
     3301 aaagaaaagc tcctccaaaa gaaagcctat cagccagacc tagtcaagct tctggtggaa
     3361 aaaagacaat tcaagtcctt ccttggcgac ctctcaagtg cctctgataa attgctagag
     3421 gagaaactag ctactgttcc tgctcccttt tgtgaggagg aagtgctcac tcccttttca
     3481 agactgacag tagattctca cctgagtagg tcagctgaag atagctttct gtcacccatc
     3541 atctcccagt ctagaaagag caaaattcca aggccagttt catgggtcaa cacagatcag
     3601 gtcaatagct caacttcgtc tcagttcttt cctcggccac caccaggaaa gccacccacg
     3661 aggcctggag tagaagccag gctacgcaga tataaagtcc tagggagtag taactccgac
     3721 tcagaccttt tctcccgcct ggcccaaatt cttcaaaatg gatctcagaa accccggagc
     3781 actactcagt gcaagagtcc aggatctcct cacaatccaa aaacaccacc caagagtcca
     3841 gttgtccctc gcaggagtcc cagtgcctct cctcgaagct catccttgcc tcgcacgtct
     3901 agttcctcac catctagggc tggacggccc caccatgacc agaggagttc gtccccacat
     3961 ctggggagaa gcaagtcacc tcccagccac tcaggatctt cctcctccag gaggtcctgc
     4021 caacaggagc attgcaaacc cagcaagaat ggcctgaaag gatccggcag cctccaccac
     4081 cactcagcca gcactaaaac cccccaaggg aagagtaagc cagccagtaa actcagcaga
     4141 taggagccag gctgcatctc tttgaaaggt gtgagatctt cctcctaaac ctgatgcatg
     4201 tgtgtccctg tactttctat gtaaaaaaat cagtgttgat cttctcttgc aaaagaaagt
     4261 aacatgatca attatttata agaagacata atacatgata aggaattacc taaggcaggc
     4321 agcaagtaga ttaggaatca atgtctttgt acaagaagga aaaatagagc aaaaatccaa
     4381 gggggagaaa ctcattaaaa tgagctctca ttttttaagc tgcctttgaa acaaaagagt
     4441 tgaggatagg agatagaatg gaattttagg ggggttgcct aattttttta agcctcaatt
     4501 caaagattat atagcaaaag tgaaacttct tgtttgatat tttcattcaa aactttccca
     4561 ccctgaagag tcattgatca gatattagat tatataagaa gtctgttgcc agggagccag
     4621 tattcatgta tatttggctt gtgtgtttat ttcgtgtatt gagaatgaac acctttactt
     4681 tgcctcattc ctagtaccct ccctggagtt cagatttttt tttaaaattt tgtatgtctc
     4741 gtctgattca atctctctgc ttttatttta tggtcctagt tgtactatca aatccaatta
     4801 ctttttttta ggtccccctg attttttttt tttagagcaa gagttcttaa catattacat
     4861 ttttattatg aaaaataaga aagttaggta aaggaaagaa aagtctaact agagctattt
     4921 tgcaggcttt agtgtttagg gagagaaaga aagtgtgggt taatagcctt caagatagaa
     4981 gatgcccttt catctctgtt aagtgtcctc ctttagaaac ttgagtagaa ggaaaactga
     5041 ccagagtaga ctgcttcctt aagtcttctg ggttccaact gtttgtaata tcagcatcca
     5101 agatgatacg agggaagcac aatgctttgg actgtgattt gagatttaga aataaattag
     5161 atatattatt gaggcttaga atcctcaaac tttgtatttt atacatttag ccaataagga
     5221 attaatatct ggggaaataa atttaggcaa atatttcttt tttaatgttt tattacctgc
     5281 ttctcctgtg ttttagttca acatttgggc ttcttggcct gattttcata caatctcaat
     5341 ttacgaagct gtaaagagga agatatttgt tctaatctca ctcttctaat aggaatcagg
     5401 caaatgaaag tctaccagac ttttaaaatg ggctgttttt atactctcta ggtgttttgt
     5461 gttgtaaaga ccttattaag gtcaggtaaa ttggtctgct tgctgttgaa atttgccttc
     5521 tagcaaacat atgtgctttc tgtttgacct tgtgtttgct gccaaaccta atacagttga
     5581 attgggaaac aaaaaaaaaa gaaaggaata catttcctcc ccaagtgaac atcttctaat
     5641 gctgcatcaa agctgccctg aagctgcact gaacttctct tgttctcttt atctgtgttg
     5701 agctttttaa aaaaacaaac tcaaaacact attgaggcat ataacgtctc ttataagaaa
     5761 tgtagcatag tgtggaatct taatttctct ccagtttcaa acactccaga ggaatgcaat
     5821 agataagaca tacttgctgt ttatctaaag caactgtaat attggaagat cagtccttct
     5881 gtattatatt gtataatagt tgctataaca ctacttgcat gtcttcatgg taaattatat
     5941 aaatatttat aaatatatag agagacatat ccttatatag agtcattctt tgtcattctc
     6001 catttgtaat ttcgagatca cagatggcga aattcctttt ctattgtgtg cctcatgtac
     6061 acttgggcct tgcctatctt cattttctga aactatgttc ttggcatgtg acacctcaga
     6121 gtcttcttgc cttccctgtg tacaatgcaa gggtgggcag tatttaacaa gccaagatag
     6181 gttatttaat tttcatttca aaaagatttt aatagggaaa aaattacagg gacatgcttg
     6241 taatactggt tcagagattt gtcatgttaa tcacatagtt ctctccttat agttagagtg
     6301 gataaactgg gatgatgatg agtctgacag atttttaaaa catttcttaa aaattgccat
     6361 tctactttcc tcttttcatc tcactttttt tgcagggggt cgggggaata atgaagaaat
     6421 tgtcatattt ctgttgcatt agctaaattg tgctaaaagt agctagcttt aaatcctgac
     6481 ccctttagtc ggaaatatgc tagtagtaat cattggaaaa gctgatgaag ctacaactgt
     6541 ggtacgtgtg tgtgatttta tttcttattg gcatttctct gctggcagtt ggaactgaca
     6601 gtgatgggaa tgtggcaact gaatgttttc tctctttctt ggttccttcc atttttatct
     6661 caacttttct agtgtgttgt ccccttttct aaatggcatt gacaaactca tcaagatcca
     6721 gagataaaca gtccatcgtt taagaatatt ttattttgca tgtagaggaa agaattaacc
     6781 aaagacgttt ctgctctgat actttctaaa gaaaacagac taattatata taagtggtat
     6841 caataggtct gcttataagt attgtcctat gtagagattt actttttggt ttttacaagc
     6901 atagtttgcg cttttagatg tctctcaggt gaaaaataga agttggcagc cctgagcaag
     6961 cacctgtgac acagggattt ttattttagt tttaaagtat gatacagaga atgaggtttt
     7021 tggtttgatt tggtttggtt gtgtttttta gttctttatt ttttatttgg gggttctctt
     7081 agtgatggca tatttcaggt ttcagtgaca tagatcaagt aattttaaac caatttggtt
     7141 attctatcta agatgaggga catggctatt aaggtcaagc caaactatac taaaagtagt
     7201 atagggcagc agttaaaatt atcttttgaa aatcaagtat tacctggttt cttctgtcac
     7261 aacagaatag ctggttacct agtcagtcac agttgccctt gccttccctt gttagtccct
     7321 ggaggtactt gagtggaaca gaaggtagaa ttagcaacag ctcaatcact ttaggtagca
     7381 tttctcctga attctgctgc caaatcctca gggtctatgg attggttgaa atagtaaaat
     7441 cacacatagt gatttcttgc acagcgtaga ggcgtttaat tttagtgtat agtgaacaaa
     7501 gaaggaaaac tgggtcatat aaaatttaac cttaaaacac agattataaa tatctttgaa
     7561 tgttaagccc taggcagaca gacactctca ttcttaactc tggtatgcaa gctggccttg
     7621 ctgacagtgg catagttaca ttaactgttt tttaagttgc atgttgtgat gtgaatgttt
     7681 tttgtaaaag ttgccaagtt aatcgagtcc taaataagta gccaaatggt aactgagacc
     7741 cactgtagac attactcgct ttatattatt tgctttcaaa tactaaaacc tcagctgcta
     7801 attcagcgct ctaggtgtac agtgtctcct tcccttggct cctagcaatg tagggaactt
     7861 gaggttcttc agtatcttca gagattcctt ccaggccaca tatttttatg ctaatacttc
     7921 tatagttgca ttcaatctac aaggagaaag tgtacattgg tgcatgtttt ataaacccag
     7981 acacagatag ctaaaaccat aattagtttt tctataccca gagctttgaa ttaaaaacag
     8041 atgctttttt tttccccaag agcagacagt tctacattcc taacattagg aattggtgat
     8101 acgagaagca ggatccagaa ttcagaatga tttagtgaga ctcggtgaaa aaaatgcatt
     8161 ttcccctggc tgtttgaaaa tttacttatt tgcagataag tctagattta gtcttggaga
     8221 tcaaagtctt ttatatttta aaaacttatt ctttatattg atcaaacatg gcatatgtta
     8281 gagaaccact tcttctgtca tgtttatgta ttttggaatt aagttgtttg cattcacttt
     8341 caaaatctgc ccatttctgt ttatgtgcac ttaccacaga tgtgtcggga ctttgcctca
     8401 ggggagaggt actttagcac ctgtgtcact gaggagatgg agtggttgac aagtactgtt
     8461 gcgctgtgta acttggggtt tggccctgtg gacaatatat tagcagaatg ataccacaca
     8521 aaagtattac aggattaagg catgtaactt ctatggtagt ccttatgtat cagcgtatac
     8581 ccaagttcag aaaccacagg tgcattttta gacctttact tagagaacta aaggcagttc
     8641 caaccatcag cccatatggc gggattaatg catgaaaacc ctcagagggt gttgggacat
     8701 cctacttccc tgtcctcacc cagtggaact ctggtgtgtg ccttgaggat aaggaagtag
     8761 agtggaaact catcctatca ttgagtattc tcaatatttt ggccttccct ctggaattat
     8821 gagaaattta acaaagtctc aggaaccttt agaatccatt gtccaacact gctagaaaaa
     8881 ctgtaggagg tacatggaga attcctatag ttcttaggta agtgcaagac atggcacagg
     8941 gatccctatc cacataaagg ggaatctgga tgctgcacac ctcaattctg agaaatccct
     9001 gactgaactt ggaattatga cagtaaagtt ttcgtccttt agttttctag agcagctcac
     9061 agaaatttta aaaagtaaaa caaggccagg cgcagtggct catgcctgta atcccagctc
     9121 tttgggaggc tgaggcgggc agatcacgag gtgaggagat cgagaccatc ctggctaaca
     9181 gggtgaaacc ccgtctctac tgaaaataca aaaaattagc tgggcatggt ggcgggcgcc
     9241 tgtagtccca gatgctcagg aggctgaggc aggagaatcg cttgaacctg ggaggcagaa
     9301 gattacagta agccaagatc gccccactgc actccagcct gggcgacaga gtgagactcc
     9361 gtctcaaaaa aaaaaaaaaa aaaaagtaaa acaaaaataa agtctatgcc cattaagacg
     9421 tcttctaatt cagttgtgat tgtctgctcc tacttaaaaa aatatttaag cttgatgttt
     9481 aattattccc tttcagcaaa tttggatcag aaaattaaag tatgtgacaa gatcaggtca
     9541 ccttgaattt ccacacaatc tcaagacact gaatagcaaa aaagtaacat tacacagtaa
     9601 tgattaggat atttccttag actttgctgg atctttggtc ttaaggtaac atgtaaaagt
     9661 agtgaagcct ttcctttcat ggccctgtgc aatgtaacgg ttttctgcct cctcttcagc
     9721 tggaagcgtt agtggtagta tgggcacaga atatatgtac actggcgatg ctgaccatgc
     9781 ctcccaggta ccctggctct gggttccttg acctagggaa caagattgga tgaggcagat
     9841 ctttgagccc atgtgactat agaatttgct gatgatataa ttttacaata acaatggata
     9901 ggaattttac ctctcttttt attagtttaa tattatttaa tattatgtac ataagtgttc
     9961 actcgcctaa ttaaaaacat tgagtaaacc aagtttttat atagactacc cttgccatat
    10021 gatgctcttt ttctctaata atatgcagtt taaatcctga ggaatcaatg cccagcattt
    10081 caccacatct gaactctgtg tgggcattct tcactcgcct acaaggggta aacaaggcta
    10141 ccagaacttg aatttgactt atagggagct acccaggaag gggaaagccc ttgggacttt
    10201 ttccaaaaca atcttctatt tgaactgttc atcagccaaa gtagtccact gaggtgacaa
    10261 agctttcaga aatacaaaga tgggaagata aaggtaacac tggcccactt ggggctttga
    10321 cattggattg ggtggactga ataaacacag cctaggtggc ctgggcttga gcctcactta
    10381 cttctccttg atacatagtt cctggtctac cttctgaccc tttttctaaa atagccagtg
    10441 tctatttcac taggccattt acttacaagt tcccagcttt tagggaaaaa agagggaggg
    10501 gggagcatct agttttgaat tagatataca tcttagaagt aatgagctat tggcagctgt
    10561 taaatcagat tcagccacaa accagaattc tttcttgttg aacaagacca atgagttaga
    10621 tgactttaat aattccactt ttctctccct ctcttctcct cttcctgaaa tcagagagat
    10681 gagaaactac tctttgaaat acctccagag gcgttttatt gtgttccttt cccctccaag
    10741 cagctccctt tatacaattt tgctcaggca accaaggaca gagtatcggc agaaacatgg
    10801 agtgcttttg tataggccac ctgtacataa aagtgtaatt atttatttaa ttttcccatt
    10861 tgtatcatat taaagctttg tacagtgttt taagttctgt tttaaaatta ttttgtattt
    10921 tatttttata acctagtaat aaaatattca ttccgcatgc aaaatctagt tctgtttgtg
    10981 tgatggtctg gatttcaaaa gtggaaaata tttttctaat ttaataaagt tattgaatac
    11041 accagatgtt acaagatcaa cggggagcag atagtgttac tgtaaatgca gtaccacatc
    11101 tagaagttcc ctagaaaaag cagcccagga ctgaatagaa gctaggtgtt aagtgtccct
    11161 gcagttagga gatgttttcc tgtaataaaa ttaaaatatt aaaagctc
//
