LOCUS       NM_032538               7130 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens tau tubulin kinase 1 (TTBK1), mRNA.
ACCESSION   NM_032538 XM_166453
VERSION     NM_032538.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Bashore,F.M., Marquez,A.B., Chaikuad,A., Howell,S., Dunn,A.S.,
            Beltran,A.A., Smith,J.L., Drewry,D.H., Beltran,A.S. and Axtman,A.D.
  TITLE     Modulation of tau tubulin kinases (TTBK1 and TTBK2) impacts
            ciliogenesis
  JOURNAL   Sci Rep 13 (1), 6118 (2023)
   PUBMED   37059819
  REMARK    GeneRIF: Modulation of tau tubulin kinases (TTBK1 and TTBK2)
            impacts ciliogenesis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Ahamad,S., Hema,K., Kumar,V. and Gupta,D.
  TITLE     The structural, functional, and dynamic effect of Tau tubulin
            kinase1 upon a mutation: A neuro-degenerative hotspot
  JOURNAL   J Cell Biochem 122 (11), 1653-1664 (2021)
   PUBMED   34297427
  REMARK    GeneRIF: The structural, functional, and dynamic effect of Tau
            tubulin kinase1 upon a mutation: A neuro-degenerative hotspot.
REFERENCE   3  (bases 1 to 7130)
  AUTHORS   McMillan,P., Wheeler,J., Gatlin,R.E., Taylor,L., Strovas,T.,
            Baum,M., Bird,T.D., Latimer,C., Keene,C.D., Kraemer,B.C. and
            Liachko,N.F.
  TITLE     Adult onset pan-neuronal human tau tubulin kinase 1 expression
            causes severe cerebellar neurodegeneration in mice
  JOURNAL   Acta Neuropathol Commun 8 (1), 200 (2020)
   PUBMED   33228809
  REMARK    GeneRIF: Adult onset pan-neuronal human tau tubulin kinase 1
            expression causes severe cerebellar neurodegeneration in mice.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7130)
  AUTHORS   Li,J., Chen,W., Yi,Y. and Tong,Q.
  TITLE     miR-219-5p inhibits tau phosphorylation by targeting TTBK1 and
            GSK-3beta in Alzheimer's disease
  JOURNAL   J Cell Biochem 120 (6), 9936-9946 (2019)
   PUBMED   30556160
  REMARK    GeneRIF: miR-219-5p inhibits tau phosphorylation by targeting TTBK1
            and GSK-3beta in Alzheimer's disease.
REFERENCE   5  (bases 1 to 7130)
  AUTHORS   Taylor,L.M., McMillan,P.J., Liachko,N.F., Strovas,T.J., Ghetti,B.,
            Bird,T.D., Keene,C.D. and Kraemer,B.C.
  TITLE     Pathological phosphorylation of tau and TDP-43 by TTBK1 and TTBK2
            drives neurodegeneration
  JOURNAL   Mol Neurodegener 13 (1), 7 (2018)
   PUBMED   29409526
  REMARK    GeneRIF: Our findings suggest a possible etiology for the two most
            common frontotemporal lobar degeneration subtypes through a TTBK1/2
            activation driven mechanism of neurodegeneration
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7130)
  AUTHORS   Vazquez-Higuera,J.L., Martinez-Garcia,A., Sanchez-Juan,P.,
            Rodriguez-Rodriguez,E., Mateo,I., Pozueta,A., Frank,A.,
            Valdivieso,F., Berciano,J., Bullido,M.J. and Combarros,O.
  TITLE     Genetic variations in tau-tubulin kinase-1 are linked to
            Alzheimer's disease in a Spanish case-control cohort
  JOURNAL   Neurobiol Aging 32 (3), 550 (2011)
   PUBMED   20096481
  REMARK    GeneRIF: This study demonistrated that TTBK1 is a promising new
            candidate tau phosphorylation-related gene for Alzheimer's disease
            risk.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 7130)
  AUTHORS   Sato,S., Xu,J., Okuyama,S., Martinez,L.B., Walsh,S.M.,
            Jacobsen,M.T., Swan,R.J., Schlautman,J.D., Ciborowski,P. and
            Ikezu,T.
  TITLE     Spatial learning impairment, enhanced CDK5/p35 activity, and
            downregulation of NMDA receptor expression in transgenic mice
            expressing tau-tubulin kinase 1
  JOURNAL   J Neurosci 28 (53), 14511-14521 (2008)
   PUBMED   19118186
  REMARK    GeneRIF: TTBK1 in AD brain may be one of the underlying mechanisms
            inducing CDK5 and calpain activation, NR2B downregulation, and
            subsequent memory dysfunction.
REFERENCE   8  (bases 1 to 7130)
  AUTHORS   Sato,S., Cerny,R.L., Buescher,J.L. and Ikezu,T.
  TITLE     Tau-tubulin kinase 1 (TTBK1), a neuron-specific tau kinase
            candidate, is involved in tau phosphorylation and aggregation
  JOURNAL   J Neurochem 98 (5), 1573-1584 (2006)
   PUBMED   16923168
REFERENCE   9  (bases 1 to 7130)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   10 (bases 1 to 7130)
  AUTHORS   Takahashi,M., Tomizawa,K., Sato,K., Ohtake,A. and Omori,A.
  TITLE     A novel tau-tubulin kinase from bovine brain
  JOURNAL   FEBS Lett 372 (1), 59-64 (1995)
   PUBMED   7556643
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL133375.25, AB218664.1,
            AK131217.1 and BC036764.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_032538.2.
            
            Summary:This gene belongs to the casein kinase 1 superfamily. The
            encoded protein is a neuron-specific, serine/threonine and tyrosine
            kinase, which regulates phosphorylation of tau, a protein that
            associates with microtubule assemblies and stabilizes them. Genetic
            variants in this gene are associated with Alzheimer's disease.
            [provided by RefSeq, Jul 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB218664.1, ERR4352443.407120.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000259750.9/ ENSP00000259750.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-199               AL133375.25        65768-65966
            200-390             AB218664.1         1-191
            391-538             AK131217.1         118-265
            539-7127            AB218664.1         340-6928
            7128-7130           BC036764.1         3917-3919
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.1"
     gene            1..7130
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /note="tau tubulin kinase 1"
                     /db_xref="GeneID:84630"
                     /db_xref="HGNC:HGNC:19140"
                     /db_xref="MIM:619415"
     exon            1..228
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            229..390
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     CDS             283..4248
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /EC_number="2.7.11.1"
                     /note="brain-derived tau kinase"
                     /codon_start=1
                     /product="tau-tubulin kinase 1"
                     /protein_id="NP_115927.1"
                     /db_xref="CCDS:CCDS34455.1"
                     /db_xref="GeneID:84630"
                     /db_xref="HGNC:HGNC:19140"
                     /db_xref="MIM:619415"
                     /translation="MQCLAAALKDETNMSGGGEQADILPANYVVKDRWKVLKKIGGGG
                     FGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKF
                     NYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNF
                     AMGRLPSTYRKCYMLDFGLARQYTNTTGDVRPPRNVAGFRGTVRYASVNAHKNREMGR
                     HDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIA
                     SLDYFTKPDYQLIMSVFENSMKERGIAENEAFDWEKAGTDALLSTSTSTPPQQNTRQT
                     AAMFGVVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPILPGRPSEGLGPSPHLVPHP
                     GGPEAEVWEETDVNRNKLRINIGKSPCVEEEQSRGMGVPSSPVRAPPDSPTTPVRSLR
                     YRRVNSPESERLSTADGRVELPERRSRMDLPGSPSRQACSSQPAQMLSVDTGHADRQA
                     SGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFPPGAEPSTSGT
                     TDEEPEELRPLPEEGEERRRLGAEPTVRPRGRSMQALAEEDLQHLPPQPLPPQLSQGD
                     GRSETSQPPTPGSPSHSPLHSGPRPRRRESDPTGPQRQVFSVAPPFEVNGLPRAVPLS
                     LPYQDFKRDLSDYRERARLLNRVRRVGFSHMLLTTPQVPLAPVQPQANGKEEEEEEEE
                     DEEEEEEDEEEEEEEEEEEEEEEEEEEEEEEAAAAVALGEVLGPRSGSSSEGSERSTD
                     RSQEGAPSTLLADDQKESRGRASMADGDLEPEEGSKTLVLVSPGDMKKSPVTAELAPD
                     PDLGTLAALTPQHERPQPTGSQLDVSEPGTLSSVLKSEPKPPGPGAGLGAGTVTTGVG
                     GVAVTSSPFTKVERTFVHIAEKTHLNVMSSGGQALRSEEFSAGGELGLELASDGGAVE
                     EGARAPLENGLALSGLNGAEIEGSALSGAPRETPSEMATNSLPNGPALADGPAPVSPL
                     EPSPEKVATISPRRHAMPGSRPRSRIPVLLSEEDTGSEPSGSLSAKERWSKRARPQQD
                     LARLVMEKRQGRLLLRLASGASSSSSEEQRRASETLSGTGSEEDTPASEPAAALPRKS
                     GRAAATRSRIPRPIGLRMPMPVAAQQPASRSHGAAPALDTAITSRLQLQTPPGSATAA
                     DLRPKQPPGRGLGPGRAQAGARPPAPRSPRLPASTSAARNASASPRSQSLSRRESPSP
                     SHQARPGVPPPRGVPPARAQPDGTPSPGGSKKGPRGKLQAQRATTKGRAGGAEGRAGA
                     R"
     exon            391..538
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            539..612
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            613..753
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            754..858
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            859..924
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1017
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1143
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1298
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1530
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1706
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..2268
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..3854
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     exon            3855..7130
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7109..7114
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /note="hexamer: ATTAAA"
     polyA_site      7130
                     /gene="TTBK1"
                     /gene_synonym="BDTK"
                     /note="major polyA site"
ORIGIN      
        1 attgtgggca gctccccgct ctgccgctgc cgccgccgtc gcccaaggag gatcggggcc
       61 gggccgggcc gggatgatcc gggtcggaag gccgccgccg ccggagggag cgggtcaccc
      121 aacgccgcac tgagccgccc ccgccccgcc ccggccccgg gggatgcgcc gccccgagct
      181 gctgcctccg ccgccgccgc agccgcagcc gcagcgggca cagagcaggt agatggcccc
      241 ctcagggcag gcccggcgga cacccctccc tctggctggc ggatgcagtg cctagcggcc
      301 gcccttaagg acgaaaccaa catgagtggg ggaggggagc aggccgacat cctgccggcc
      361 aactacgtgg tcaaggatcg ctggaaggtg ctgaaaaaga tcgggggcgg gggctttggt
      421 gagatctacg aggccatgga cctgctgacc agggagaatg tggccctcaa ggtggagtca
      481 gcccagcagc ccaagcaggt cctcaagatg gaggtggccg tgctcaagaa gttgcaaggg
      541 aaggaccatg tgtgcaggtt cattggctgt ggcaggaacg agaagtttaa ctatgtagtg
      601 atgcagctcc agggccggaa cctggccgac ctgcgccgta gccagccgcg aggcaccttc
      661 acgctgagca ccacattgcg gctgggcaag cagatcttgg agtccatcga ggccatccac
      721 tctgtgggct tcctgcaccg tgacatcaag ccttcaaact ttgccatggg caggctgccc
      781 tccacctaca ggaagtgcta tatgctggac ttcgggctgg cccggcagta caccaacacc
      841 acgggggatg tgcggccccc tcggaatgtg gccgggtttc gaggaacggt tcgctatgcc
      901 tcagtcaatg cccacaagaa ccgggagatg ggccgccacg acgacctgtg gtccctcttc
      961 tacatgctgg tggagtttgc agtgggccag ctgccctgga ggaagatcaa ggacaaggaa
     1021 caggtaggga tgatcaagga gaagtatgag caccggatgc tgctgaagca catgccgtca
     1081 gagttccacc tcttcctgga ccacattgcc agcctcgact acttcaccaa gcccgactac
     1141 cagttgatca tgtcagtgtt tgagaacagc atgaaggaga ggggcattgc cgagaatgag
     1201 gcctttgact gggagaaggc aggcaccgat gccctcctgt ccacgagcac ctctaccccg
     1261 ccccagcaga acacccggca gacggcagcc atgtttgggg tggtcaatgt gacgccagtg
     1321 cctggggacc tgctccggga gaacaccgag gatgtgctac agggagagca cctgagtgac
     1381 caggagaatg cacccccaat tctgcccggg aggccctctg aggggctggg ccccagtccc
     1441 caccttgtcc cccaccccgg gggtcctgag gctgaagtct gggaggagac agatgtcaac
     1501 cggaacaaac tccggatcaa catcggcaaa agcccctgtg tggaggagga acagagccga
     1561 ggcatggggg tccccagctc cccagtgcgt gcccccccag actcccccac aaccccagtc
     1621 cgttctctgc gctaccggag ggtgaacagc cctgagtcag aaaggctgtc cacggcggac
     1681 gggcgagtgg agctacctga gaggaggtca cggatggatc tgcctggctc gccctcgcgc
     1741 caggcctgct cctctcagcc agcccagatg ctgtcagtgg acacaggcca cgctgaccga
     1801 caggccagtg gccgcatgga cgtgtcagcc tctgtggagc aggaggccct gagcaacgcc
     1861 ttccgctcgg tgccgctggc tgaggaggag gatttcgaca gcaaagagtg ggtcatcatc
     1921 gacaaggaga cggagctcaa ggacttccct ccaggggctg agcccagcac atcgggcacc
     1981 acggatgagg agcccgagga gctgcggcca ctgcccgagg agggcgaaga gcggcggcgg
     2041 ctgggggcag agcccaccgt ccggccccgg ggacgcagca tgcaggcgct ggcggaggag
     2101 gacctgcagc atttgccgcc ccagcccctg ccaccccagc tgagccaggg cgatggccgt
     2161 tccgagacgt cacagccccc cacgcctggc agcccttccc actcacccct gcactcggga
     2221 ccccgccctc gacggagaga gtcggacccc acaggcccac agagacaggt gttctccgtg
     2281 gcgcccccat ttgaggtgaa tggcctccca cgagctgtgc ctctgagtct gccctaccag
     2341 gacttcaaaa gagacctctc cgattaccga gaacgggcgc ggttgctcaa cagggtccgg
     2401 agggtgggct tctcgcacat gctgctcacc accccccagg tcccactggc tcctgttcag
     2461 cctcaggcta atgggaagga ggaagaggag gaggaggagg aagatgagga agaggaagaa
     2521 gaggatgagg aagaagaaga ggaggaagag gaagaggagg aggaagaaga ggaggaggag
     2581 gaagaggagg aggaggctgc agcggcagtt gccttggggg aggtgctggg gcctcgtagt
     2641 ggctccagca gtgaggggag tgagaggagc actgaccgga gccaggaggg tgccccgtcc
     2701 acgctgctgg cagacgatca gaaggagtcc aggggccggg cctccatggc cgatggggac
     2761 ctggagcctg aggagggctc caaaacgctg gtgcttgtct ctcctggcga catgaagaag
     2821 tcgcccgtca ctgccgaact ggcccccgac cccgacctgg gcaccctggc tgccctcact
     2881 cctcagcatg agcggcccca gcccacgggc agccagctgg acgtatctga gccaggcacc
     2941 ctgtcctctg tcctcaagtc tgagcccaag cccccggggc ctggggcagg gctgggggcc
     3001 gggacagtga ccacaggggt cgggggcgtg gcagtcacct cctcaccctt caccaaagtt
     3061 gagaggacct ttgtgcacat tgcggagaaa acccacctca acgtcatgtc ttccggtgga
     3121 caagccttgc ggtctgagga gttcagcgct gggggcgagc tgggtctgga gctggcctct
     3181 gatgggggcg ctgtggagga gggggcccga gcgcccctgg agaacggcct cgccctgtca
     3241 gggctgaatg gggctgagat agagggctct gccctgtctg gggccccccg ggaaaccccc
     3301 tcagagatgg ccacaaactc actgcccaat ggcccggccc ttgcagacgg gccagccccg
     3361 gtgtccccgc tggagccaag ccctgagaaa gtggccacca tctcccccag acgccatgct
     3421 atgccaggct ctcgccccag gagccgtatc cctgtcctgc tctctgagga ggacacgggc
     3481 tcggagccct caggctcact gtcggccaaa gagcggtgga gcaagcgggc tcggccgcag
     3541 caggacctgg cgcggctggt gatggagaag aggcagggcc gcctgctgtt gcggctggcc
     3601 tcaggggcct cgtcctcctc cagtgaggag cagcgccgtg cctctgagac cctctcaggc
     3661 acgggctctg aggaggacac gcccgcctct gagccggcag cggccttgcc caggaagagc
     3721 gggagggcag ccgccaccag gagccggatt ccccgcccca ttggcctccg catgcccatg
     3781 cctgttgcag cccagcagcc cgccagcaga tcccatggcg cggccccagc attggacaca
     3841 gccatcacca gcaggctcca gctgcagacg cccccagggt cggccactgc tgctgacctc
     3901 cgccccaaac aacctcctgg ccgcggcctg ggcccagggc gagcccaagc cggagccagg
     3961 cccccagcgc cgcgcagccc gcgcctcccc gcgtccacat ccgccgcgcg caatgccagc
     4021 gcgtcccccc ggagccagtc cctgtcccgc agagagagcc cctccccctc gcaccaggcc
     4081 cggcccgggg tccccccgcc ccggggcgtc ccgccggccc gggcccagcc tgatggcacc
     4141 ccctcccccg ggggctccaa gaaaggaccc agagggaaac tccaggctca gcgcgcaaca
     4201 accaaaggcc gggcaggagg cgcggagggc cgggctgggg ccagataatg acgcccgctg
     4261 ctctccgcgg tcccccaccc tcaccccggc cccccacccg cagccggcca cactggagca
     4321 gctcccagca cagccttacg cgcccgacgc gcgccacccg cggccccagc tttccgcctg
     4381 cacccgcgag gacgcgcgcg agcacacgcg gcgccccgcc aggccttagg gcccgtgggg
     4441 gacgcggccc cgcgccgcgg ggagggtctg cctccccttc ctcgccctgt gtcctctcat
     4501 cctcccgccg cccgtcaggc cggccagcct cacatcagtc tctccgcccc ggggaaggct
     4561 cagccacttt tcatcgagga ctccacttct ggggacgcct ggttcgttcg cccaccaggc
     4621 ctaggctacg ctccatgctc ccccagcaat ctctgcctac acctcctgcg gcgccttgcc
     4681 ctcctccgac ccctttccag ccaaagtccc cccacccctt cagagaagca gcctcaaatt
     4741 ccagaagtgg aggctccagc ctccccgcga gggtccagcc ccacagtctt ctgggagcca
     4801 ttgtggccag ggacggcctc tggactgcca ggctgggttg gggacccagg gaacatcggt
     4861 ctactcaggt gtgagggggc aggtctgacc tgccccaaag ttggctccat cctggacaac
     4921 tcggtgagag gcagtgggca agtgatcttg gagatgggtg ggcaggtgat tctgtgggca
     4981 ggggatgtgc tcccctgcac ctctggggtg cagaaacctc ttgcctccag atttgggtgg
     5041 agcctctgtg ggaaccatag gaagtgtgtg ggctgccttc ctgggcaagt atttcccagt
     5101 gggaagttgg agggggcttt aacaaagttt tactccctcc cctgttcccc tgatctagtg
     5161 ctcaggaccc ttcaccatca ggaattcctt cctgtcatct aacctcagtc ctgcctactg
     5221 cagttccagc caacctgctc tttcctgagt tcaaagcagg tggagactgg ctggttacca
     5281 tctttgcact ggcccttcgg agattcgggg actcagttct ggtggggtca ccctccctgt
     5341 cctcccgcct gtgggaggga gggagggctg gctcaggcat cgtctcccgc aatgggcaga
     5401 gagagcagag acaggtggac caacagacag ctggcccctg gaggcagaaa ggcccttcta
     5461 acttccagat tgtatgcttg agtgatgggt ccccagccca agcccactct tccctcagct
     5521 cacccttcag cctgttcctt cttgccctga ccccagcccg tgcagctgct ctactccagg
     5581 aatggatgtg gggactcttc ctgggttctg gctcctgcat agctcacccc acctcatcat
     5641 gagcctcaac tgcctacatc tggggcaagc agcacaccgg ctgcagatgg gacagccagc
     5701 cctgcctatc tggacaggcc cctgcagcct ctgtcccctg gcctagcctc tctgtccttc
     5761 cctgagtcac agagagcaag ccaagacatc cagggaaaga ggaagaaagg ccttagtgtg
     5821 ccccagcagt ctggctgcgt ccagccacca tcacccggaa ggatgcccac aaggcagctg
     5881 accctgaaag cagcctcccc ctcatggaga gtcagcagct tgggcagcca cttccaggcc
     5941 agggtggtgg cttctctgca gaccagctga ggggaggact cctgggtgga cagcctttga
     6001 cgtccacccc acgctgatgc agaagctccc agaacactca ggaaacttct ccggacagag
     6061 ccctccttgt caacttgagg ccctcccaag gccctctact gccctctggg tccagcagag
     6121 ggagtggagg aagggccact gcctcccacc tagagcttct ccgaatgaca atcagctcgt
     6181 gccaggtggg gaccaggata tgactcctgg tgcccaggcc ctgggcctgc tccttgccac
     6241 caaccgaacc gtgaatgtag ggcccccagc ctcacctctg ccccaggacc aacaacaccc
     6301 tggtttggag ctgggaggaa gaagggggcc tgagagagcc ccaggtccat tctaccccca
     6361 gcttcactca gcactggagc tggcagagac gcaaaaccca gtctgccctt gggattccaa
     6421 acctccctag ggctcccaac tgacctcagg cctctgagtc actgaatgtc accaggagag
     6481 gtgggggagg gaaagtgggc cagtggggag ggggtcacct aggggactgc ctctgtgcct
     6541 ctccccagga agcatccagg gcagaggaag ccacatctcc cggtgccccc aaccccagct
     6601 gcagcctcct ccccctgagc attcattctc tccaccaggc ctccaggtcc tgagcccttc
     6661 ctctgtaaaa gtgtcacacc acctccctca gcacttcccc atcacaacaa cctatgtcac
     6721 tgactcagat gcagggtctg ctcaccccaa cacatgcctt ccctccccag ccacaccgtg
     6781 cacgaagggg gcacaggaga ggagaggggc tgtgccccag gctccccatt tcccagctcc
     6841 tcacagaggc ctggtttgct cagtcttctg aactccaggg accagccctg gtgggcatgg
     6901 ggtggggagc agggagttgc ccttcccctc cctcgggaag ccacctaaga atgtttacat
     6961 gccaaacaga atgtaacacc cctccccaag cccttcccag tcactgcatg gcctctgccc
     7021 atcctgcacc tgtccacccc accccaacac cctggaagcc actgtcaatg attagatcgg
     7081 gtctcggaag ggaagtagcc atcacaccat taaaaagcct gtggaccttt
//
