LOCUS       NM_001184739            1347 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens testis specific serine kinase 4 (TSSK4), transcript
            variant 1, mRNA.
ACCESSION   NM_001184739
VERSION     NM_001184739.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Shen,Y., Zhang,F., Li,F., Jiang,X., Yang,Y., Li,X., Li,W., Wang,X.,
            Cheng,J., Liu,M., Zhang,X., Yuan,G., Pei,X., Cai,K., Hu,F., Sun,J.,
            Yan,L., Tang,L., Jiang,C., Tu,W., Xu,J., Wu,H., Kong,W., Li,S.,
            Wang,K., Sheng,K., Zhao,X., Yue,H., Yang,X. and Xu,W.
  TITLE     Loss-of-function mutations in QRICH2 cause male infertility with
            multiple morphological abnormalities of the sperm flagella
  JOURNAL   Nat Commun 10 (1), 433 (2019)
   PUBMED   30683861
  REMARK    Erratum:[Nat Commun. 2019 May 20;10(1):2289. doi:
            10.1038/s41467-019-10313-x.. PMID: 31110204]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Wang,X.L., Wei,Y.H., Fu,G.L. and Yu,L.
  TITLE     Testis specific serine/threonine protein kinase 4 (TSSK4) leads to
            cell apoptosis relying on its kinase activity
  JOURNAL   J Huazhong Univ Sci Technolog Med Sci 35 (2), 235-240 (2015)
   PUBMED   25877358
  REMARK    GeneRIF: TSSK4 was able to induce cell apoptosis in vitro depending
            on its kinase activity, which leads to abnormal testes weight and
            apoptosis, shedding light on its function in the process of
            spermatogenesis and/or spermiogenesis
REFERENCE   3  (bases 1 to 1347)
  AUTHORS   Wang,X., Wei,Y., Fu,G., Li,H., Saiyin,H., Lin,G., Wang,Z., Chen,S.
            and Yu,L.
  TITLE     Tssk4 is essential for maintaining the structural integrity of
            sperm flagellum
  JOURNAL   Mol Hum Reprod 21 (2), 136-145 (2015)
   PUBMED   25361759
REFERENCE   4  (bases 1 to 1347)
  AUTHORS   Wang,J., Huo,K., Ma,L., Tang,L., Li,D., Huang,X., Yuan,Y., Li,C.,
            Wang,W., Guan,W., Chen,H., Jin,C., Wei,J., Zhang,W., Yang,Y.,
            Liu,Q., Zhou,Y., Zhang,C., Wu,Z., Xu,W., Zhang,Y., Liu,T., Yu,D.,
            Zhang,Y., Chen,L., Zhu,D., Zhong,X., Kang,L., Gan,X., Yu,X., Ma,Q.,
            Yan,J., Zhou,L., Liu,Z., Zhu,Y., Zhou,T., He,F. and Yang,X.
  TITLE     Toward an understanding of the protein interaction network of the
            human liver
  JOURNAL   Mol Syst Biol 7, 536 (2011)
   PUBMED   21988832
  REMARK    Erratum:[Mol Syst Biol. 2017 Dec 18;13(12):965. doi:
            10.15252/msb.20178107.. PMID: 29254952]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1347)
  AUTHORS   Aston,K.I., Krausz,C., Laface,I., Ruiz-Castane,E. and Carrell,D.T.
  TITLE     Evaluation of 172 candidate polymorphisms for association with
            oligozoospermia or azoospermia in a large cohort of men of European
            descent
  JOURNAL   Hum Reprod 25 (6), 1383-1397 (2010)
   PUBMED   20378615
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 1347)
  AUTHORS   Su,D., Zhang,W., Yang,Y., Deng,Y., Ma,Y., Song,H. and Zhang,S.
  TITLE     Mutation screening and association study of the TSSK4 Gene in
            Chinese infertile men with impaired spermatogenesis
  JOURNAL   J Androl 29 (4), 374-378 (2008)
   PUBMED   18390560
  REMARK    GeneRIF: association of some single nucleotide polymorphisms in
            TSSK4 with male infertility.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 1347)
  AUTHORS   Zeng,M., Deng,W., Wang,X., Qiu,W., Liu,Y., Sun,H., Tao,D., Zhang,S.
            and Ma,Y.
  TITLE     DAZL binds to the transcripts of several Tssk genes in germ cells
  JOURNAL   BMB Rep 41 (4), 300-304 (2008)
   PUBMED   18452650
REFERENCE   8  (bases 1 to 1347)
  AUTHORS   Chen,X., Lin,G., Wei,Y., Hexige,S., Niu,Y., Liu,L., Yang,C. and
            Yu,L.
  TITLE     TSSK5, a novel member of the testis-specific serine/threonine
            kinase family, phosphorylates CREB at Ser-133, and stimulates the
            CRE/CREB responsive pathway
  JOURNAL   Biochem Biophys Res Commun 333 (3), 742-749 (2005)
   PUBMED   15964553
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL136295.3, AK131042.1 and
            AY461663.1.
            
            On May 17, 2019 this sequence version replaced NM_001184739.1.
            
            Summary: This gene encodes a member of the testis-specific
            serine/threonine kinase family. The encoded protein is thought to
            be involved in spermatogenesis via stimulation of the CREB/CRE
            responsive pathway through phosphorylation of the cAMP responsive
            element binding protein transcription factor. Alternative splicing
            results in multiple transcript variants.[provided by RefSeq, May
            2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK131042.1, SRR5189667.180041.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000339917.10/ ENSP00000339179.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AL136295.3         4316-4339           c
            25-571              AK131042.1         1-547
            572-572             AL136295.3         3408-3408           c
            573-1345            AK131042.1         549-1321
            1346-1347           AY461663.1         1256-1257
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q12"
     gene            1..1347
                     /gene="TSSK4"
                     /gene_synonym="C14orf20; STK22E; TSK-4; TSK4; TSSK5"
                     /note="testis specific serine kinase 4"
                     /db_xref="GeneID:283629"
                     /db_xref="HGNC:HGNC:19825"
                     /db_xref="MIM:610711"
     exon            1..453
                     /gene="TSSK4"
                     /gene_synonym="C14orf20; STK22E; TSK-4; TSK4; TSSK5"
                     /inference="alignment:Splign:2.1.0"
     CDS             229..1245
                     /gene="TSSK4"
                     /gene_synonym="C14orf20; STK22E; TSK-4; TSK4; TSSK5"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine kinase 22E; serine/threonine-protein
                     kinase 22E"
                     /codon_start=1
                     /product="testis-specific serine/threonine-protein kinase
                     4 isoform 1"
                     /protein_id="NP_001171668.1"
                     /db_xref="CCDS:CCDS53890.1"
                     /db_xref="GeneID:283629"
                     /db_xref="HGNC:HGNC:19825"
                     /db_xref="MIM:610711"
                     /translation="MGKGDVLEAAPTTTAYHSLMDEYGYEVGKAIGHGSYGSVYEAFY
                     TKQKVMVAVKIISKKKASDDYLNKFLPREIQVMKVLRHKYLINFYRAIESTSRVYIIL
                     ELAQGGDVLEWIQRYGACSEPLAGKWFSQLTLGIAYLHSKSIVHRLMPSLSAAGRDLK
                     LENLLLDKWENVKISDFGFAKMVPSNQPVGCSPSYRQVNCFSHLSQTYCGSFAYACPE
                     ILRGLPYNPFLSDTWSMGVILYTLVVAHLPFDDTNLKKLLRETQKEVTFPANHTISQE
                     CKNLILQMLRQATKRATILDIIKDSWVLKFQPEQPTHEIRLLEAMCQLHNTTKQHQSL
                     QITT"
     exon            454..668
                     /gene="TSSK4"
                     /gene_synonym="C14orf20; STK22E; TSK-4; TSK4; TSSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            669..1062
                     /gene="TSSK4"
                     /gene_synonym="C14orf20; STK22E; TSK-4; TSK4; TSSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1347
                     /gene="TSSK4"
                     /gene_synonym="C14orf20; STK22E; TSK-4; TSK4; TSSK5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1317..1322
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSSK4"
                     /gene_synonym="C14orf20; STK22E; TSK-4; TSK4; TSSK5"
                     /note="hexamer: AATAAA"
     polyA_site      1347
                     /gene="TSSK4"
                     /gene_synonym="C14orf20; STK22E; TSK-4; TSK4; TSSK5"
                     /note="major polyA site"
ORIGIN      
        1 aaagtaacaa cagccagtgg agacaaaaag aactgcttct ctttctttcc ccctccaagt
       61 tcctagtgga gggctgagtc cagcatccca gactcgtgtg actatatagg caagcatttg
      121 gggacctact tcactttgat accctagcct tcagcagctc aaggtgttgg cctttggata
      181 ggaggcttcc aagtagtaaa gctccctgct ctcagcaagc ccaacaccat ggggaaggga
      241 gatgtcttag aggcagcacc aaccaccaca gcctaccatt ccctcatgga tgaatatggt
      301 tatgaggtgg gcaaggccat tggccatggc tcctatgggt cggtatatga ggctttctac
      361 acaaagcaga aggttatggt ggcagtcaag atcatctcaa agaagaaggc ctctgatgac
      421 tatcttaaca agttcctgcc ccgtgaaata caggtaatga aagtcttgcg gcacaagtac
      481 ctcatcaact tctatcgggc cattgagagc acatctcgag tatacatcat tctggaactg
      541 gctcagggtg gtgatgtcct tgaatggatc cagcgctacg gggcctgctc tgagcccctt
      601 gctggcaagt ggttctccca gctgaccctg ggcattgcct acctgcacag caagagcatc
      661 gtgcaccgcc tgatgcccag cctttctgct gctggtaggg acttaaagtt ggagaacctg
      721 ttgctggaca agtgggagaa tgtgaagata tcagactttg gctttgccaa gatggtgcct
      781 tctaaccagc ctgtgggttg tagcccttct taccgccaag tgaactgctt ttcccacctc
      841 agccagactt actgtggcag ctttgcttac gcttgcccag agatcttacg aggcttgccc
      901 tacaaccctt tcctgtctga cacctggagc atgggcgtca tcctttacac tctagtggtc
      961 gcccatctgc cctttgatga caccaatctc aaaaagctgc taagagagac tcagaaggag
     1021 gtcactttcc cagctaacca taccatctcc caggagtgca agaacctgat cctccagatg
     1081 ctacgccaag ccactaagcg tgccaccatt ctggacatca tcaaggattc ctgggtgctc
     1141 aagttccagc ctgagcaacc cacccatgag atcaggctgc ttgaggccat gtgccagctc
     1201 cacaacacca ctaaacagca ccaatccttg caaattacga cctgaaaatg gctgagggag
     1261 ggggctaaga gaggagcaaa gcaggaggtc ttgggctaaa aatctttttt accaaaaata
     1321 aatctaagtc tgatttagtt tcatcaa
//
