LOCUS       NM_053006               1315 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens testis specific serine kinase 2 (TSSK2), mRNA.
ACCESSION   NM_053006
VERSION     NM_053006.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   3  (bases 1 to 1315)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1315)
  AUTHORS   Shetty,J., Sinville,R., Shumilin,I.A., Minor,W., Zhang,J.,
            Hawkinson,J.E., Georg,G.I., Flickinger,C.J. and Herr,J.C.
  TITLE     Recombinant production of enzymatically active male contraceptive
            drug target hTSSK2 - Localization of the TSKS domain phosphorylated
            by TSSK2
  JOURNAL   Protein Expr Purif 121, 88-96 (2016)
   PUBMED   26777341
  REMARK    GeneRIF: n vitro phosphorylation experiments carried out with TSKS
            (isoform 1) fragments revealed particularly strong phosphorylation
            of a recombinant N-terminal region representing aa 1-150 of TSKS,
            indicating that the N-terminus of human TSKS is phosphorylated by
            human TSSK2.
REFERENCE   5  (bases 1 to 1315)
  AUTHORS   Shang,P., Hoogerbrugge,J., Baarends,W.M. and Grootegoed,J.A.
  TITLE     Evolution of testis-specific kinases TSSK1B and TSSK2 in primates
  JOURNAL   Andrology 1 (1), 160-168 (2013)
   PUBMED   23258646
  REMARK    GeneRIF: Report evolution of testis-specific kinases TSSK1B and
            TSSK2 in primates.
REFERENCE   6  (bases 1 to 1315)
  AUTHORS   Hao,Z., Jha,K.N., Kim,Y.H., Vemuganti,S., Westbrook,V.A.,
            Chertihin,O., Markgraf,K., Flickinger,C.J., Coppola,M., Herr,J.C.
            and Visconti,P.E.
  TITLE     Expression analysis of the human testis-specific serine/threonine
            kinase (TSSK) homologues. A TSSK member is present in the
            equatorial segment of human sperm
  JOURNAL   Mol Hum Reprod 10 (6), 433-444 (2004)
   PUBMED   15044604
  REMARK    GeneRIF: Data show that human testis-specific serine/threonine
            (Ser/Thr) kinase (TSSK) 1 and 2 messages are expressed exclusively
            in the testis and suggest that this family of kinases might play a
            role in sperm function.
REFERENCE   7  (bases 1 to 1315)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
REFERENCE   8  (bases 1 to 1315)
  AUTHORS   Dunham,I., Shimizu,N., Roe,B.A., Chissoe,S., Hunt,A.R.,
            Collins,J.E., Bruskiewich,R., Beare,D.M., Clamp,M., Smink,L.J.,
            Ainscough,R., Almeida,J.P., Babbage,A., Bagguley,C., Bailey,J.,
            Barlow,K., Bates,K.N., Beasley,O., Bird,C.P., Blakey,S.,
            Bridgeman,A.M., Buck,D., Burgess,J., Burrill,W.D., O'Brien,K.P. et
            al.
  TITLE     The DNA sequence of human chromosome 22
  JOURNAL   Nature 402 (6761), 489-495 (1999)
   PUBMED   10591208
  REMARK    Erratum:[Nature 2000 Apr 20;404(6780):904]
REFERENCE   9  (bases 1 to 1315)
  AUTHORS   Kueng,P., Nikolova,Z., Djonov,V., Hemphill,A., Rohrbach,V.,
            Boehlen,D., Zuercher,G., Andres,A.C. and Ziemiecki,A.
  TITLE     A novel family of serine/threonine kinases participating in
            spermiogenesis
  JOURNAL   J Cell Biol 139 (7), 1851-1859 (1997)
   PUBMED   9412477
REFERENCE   10 (bases 1 to 1315)
  AUTHORS   Gong,W., Emanuel,B.S., Collins,J., Kim,D.H., Wang,Z., Chen,F.,
            Zhang,G., Roe,B. and Budarf,M.L.
  TITLE     A transcription map of the DiGeorge and velo-cardio-facial syndrome
            minimal critical region on 22q11
  JOURNAL   Hum Mol Genet 5 (6), 789-800 (1996)
   PUBMED   8776594
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            L77564.1 and BC037781.2.
            
            On Nov 22, 2018 this sequence version replaced NM_053006.4.
            
            Summary: TSSK2 belongs to a family of serine/threonine kinases
            highly expressed in testis (Hao et al., 2004 [PubMed
            15044604]).[supplied by OMIM, Mar 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: SRR5189667.153170.1,
                                        SRR5189667.124858.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399635.4/ ENSP00000382544.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1264              L77564.1           501-1764
            1265-1315           BC037781.2         1296-1346
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.21"
     gene            1..1315
                     /gene="TSSK2"
                     /gene_synonym="DGS-G; SPOGA2; STK22B; TSK2"
                     /note="testis specific serine kinase 2"
                     /db_xref="GeneID:23617"
                     /db_xref="HGNC:HGNC:11401"
                     /db_xref="MIM:610710"
     exon            1..1315
                     /gene="TSSK2"
                     /gene_synonym="DGS-G; SPOGA2; STK22B; TSK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..1169
                     /gene="TSSK2"
                     /gene_synonym="DGS-G; SPOGA2; STK22B; TSK2"
                     /EC_number="2.7.11.1"
                     /note="testis specific serine threonine kinase 2;
                     serine/threonine kinase 22B (spermiogenesis associated);
                     spermiogenesis associated 2; TSK-2; TSSK-2;
                     testis-specific kinase 2; diGeorge syndrome protein G;
                     serine/threonine-protein kinase 22B; testicular secretory
                     protein Li 61"
                     /codon_start=1
                     /product="testis-specific serine/threonine-protein kinase
                     2"
                     /protein_id="NP_443732.3"
                     /db_xref="CCDS:CCDS13755.1"
                     /db_xref="GeneID:23617"
                     /db_xref="HGNC:HGNC:11401"
                     /db_xref="MIM:610710"
                     /translation="MDDATVLRKKGYIVGINLGKGSYAKVKSAYSERLKFNVAVKIID
                     RKKTPTDFVERFLPREMDILATVNHGSIIKTYEIFETSDGRIYIIMELGVQGDLLEFI
                     KCQGALHEDVARKMFRQLSSAVKYCHDLDIVHRDLKCENLLLDKDFNIKLSDFGFSKR
                     CLRDSNGRIILSKTFCGSAAYAAPEVLQSIPYQPKVYDIWSLGVILYIMVCGSMPYDD
                     SDIRKMLRIQKEHRVDFPRSKNLTCECKDLIYRMLQPDVSQRLHIDEILSHSWLQPPK
                     PKATSSASFKREGEGKYRAECKLDTKTGLRPDHRPDHKLGAKTQHRLLVVPENENRME
                     DRLAETSRAKDHHISGAEVGKAST"
     regulatory      1281..1286
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSSK2"
                     /gene_synonym="DGS-G; SPOGA2; STK22B; TSK2"
                     /note="hexamer: AGTAAA"
     regulatory      1295..1300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSSK2"
                     /gene_synonym="DGS-G; SPOGA2; STK22B; TSK2"
                     /note="hexamer: ATTAAA"
     polyA_site      1306
                     /gene="TSSK2"
                     /gene_synonym="DGS-G; SPOGA2; STK22B; TSK2"
     polyA_site      1315
                     /gene="TSSK2"
                     /gene_synonym="DGS-G; SPOGA2; STK22B; TSK2"
                     /note="major polyA site"
ORIGIN      
        1 agacgcctcc ggtagtgtaa atgaggacaa tgcctgctgg cccacatgac ggggggatgt
       61 agacggcagc ggcgccagtc gctcctggca ccatggacga tgccacagtc ctaaggaaga
      121 agggttacat cgtaggcatc aatcttggca agggttccta cgcaaaagtc aaatctgcct
      181 actctgagcg cctcaagttc aatgtggctg tcaagatcat cgaccgcaag aaaacaccta
      241 ctgactttgt ggagagattc cttcctcggg agatggacat cctggcaact gtcaaccacg
      301 gctccatcat caagacttac gagatctttg agacctctga cggacggatc tacatcatca
      361 tggagcttgg cgtccagggc gacctcctcg agttcatcaa gtgccaggga gccctgcatg
      421 aggacgtggc acgcaagatg ttccgacagc tctcctccgc cgtcaagtac tgccacgacc
      481 tggacatcgt ccaccgggac ctcaagtgcg agaaccttct cctcgacaag gacttcaaca
      541 tcaagctgtc tgactttggc ttctccaagc gctgcctgcg ggacagcaat gggcgcatca
      601 tcctcagcaa gaccttctgc gggtcggcag catatgcagc ccccgaggtg ctgcagagca
      661 tcccctacca gcccaaggtg tatgacatct ggagcctggg cgtgatcctg tacatcatgg
      721 tctgcggctc catgccctat gacgactccg acatcaggaa gatgctgcgt atccagaagg
      781 agcaccgtgt ggacttcccg cgctccaaga acctgacctg cgagtgcaag gacctcatct
      841 accgcatgct gcagcccgac gtcagccagc ggctccacat cgatgagatc ctcagccact
      901 cgtggctgca gccccccaag cccaaagcca cgtcttctgc ctccttcaag agggaggggg
      961 agggcaagta ccgcgctgag tgcaaactgg acaccaagac aggcttgagg cccgaccacc
     1021 ggcccgacca caagcttgga gccaaaaccc agcaccggct gctggtggtg cccgagaacg
     1081 agaacaggat ggaggacagg ctggccgaga cctccagggc caaagaccat cacatctccg
     1141 gagctgaggt ggggaaagca agcacctagc atgacaatgg ccccgttgtg tgtggtgggg
     1201 gtcggggttg gggggcatgg tgcagtcggc cttcacgtaa actaagtagg caggtaggat
     1261 ctgaagaagg cacaggtgca agtaaaattc gtcaattaaa ccactatttt gatta
//
