LOCUS       NM_001168320            4255 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens tetraspanin 9 (TSPAN9), transcript variant 2, mRNA.
ACCESSION   NM_001168320
VERSION     NM_001168320.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Qi,Y., Lv,J., Liu,S., Sun,L., Wang,Y., Li,H., Qi,W. and Qiu,W.
  TITLE     TSPAN9 and EMILIN1 synergistically inhibit the migration and
            invasion of gastric cancer cells by increasing TSPAN9 expression
  JOURNAL   BMC Cancer 19 (1), 630 (2019)
   PUBMED   31242895
  REMARK    GeneRIF: expression of the membrane protein TSPAN9 regulates
            gastric cancer cell migration and invasion by inhibiting the
            FAK-RAS-ERK1/2 signaling pathway. The extracellular secretory
            protein EMILIN1 can increase this tumor suppressive effect by
            promoting the expression of TSPAN9.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Li,P.Y., Lv,J., Qi,W.W., Zhao,S.F., Sun,L.B., Liu,N., Sheng,J. and
            Qiu,W.S.
  TITLE     Tspan9 inhibits the proliferation, migration and invasion of human
            gastric cancer SGC7901 cells via the ERK1/2 pathway
  JOURNAL   Oncol Rep 36 (1), 448-454 (2016)
   PUBMED   27177197
  REMARK    GeneRIF: The proliferation, migration and invasion of human gastric
            cancer SGC7901 cells were significantly inhibited by overexpression
            of Tspan9.
REFERENCE   3  (bases 1 to 4255)
  AUTHORS   Stiles,K.M. and Kielian,M.
  TITLE     Role of TSPAN9 in Alphavirus Entry and Early Endosomes
  JOURNAL   J Virol 90 (9), 4289-4297 (2016)
   PUBMED   26865714
  REMARK    GeneRIF: TSPAN9 is an endosomal tetraspanin whose depletion has a
            pronounced effect on the entry of several viruses that fuse in the
            early endosome.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4255)
  AUTHORS   Xie,T., Deng,L., Mei,P., Zhou,Y., Wang,B., Zhang,J., Lin,J.,
            Wei,Y., Zhang,X. and Xu,R.
  TITLE     Genome-wide association study combining pathway analysis for
            typical sporadic amyotrophic lateral sclerosis in Chinese Han
            populations
  JOURNAL   Neurobiol Aging 35 (7), 1778 (2014)
   PUBMED   24529757
REFERENCE   5  (bases 1 to 4255)
  AUTHORS   Yamaguchi,T., Nakaoka,H., Yamamoto,K., Fujikawa,T., Kim,Y.I.,
            Yano,K., Haga,S., Katayama,K., Shibusawa,T., Park,S.B., Maki,K.,
            Kimura,R. and Inoue,I.
  TITLE     Genome-wide association study of degenerative bony changes of the
            temporomandibular joint
  JOURNAL   Oral Dis 20 (4), 409-415 (2014)
   PUBMED   23746317
REFERENCE   6  (bases 1 to 4255)
  AUTHORS   Kotha,J., Zhang,C., Longhurst,C.M., Lu,Y., Jacobs,J., Cheng,Y. and
            Jennings,L.K.
  TITLE     Functional relevance of tetraspanin CD9 in vascular smooth muscle
            cell injury phenotypes: a novel target for the prevention of
            neointimal hyperplasia
  JOURNAL   Atherosclerosis 203 (2), 377-386 (2009)
   PUBMED   18799160
  REMARK    GeneRIF: tetraspanin CD9 has a role in determining vascular smooth
            muscle cell injury phenotypes
REFERENCE   7  (bases 1 to 4255)
  AUTHORS   Protty,M.B., Watkins,N.A., Colombo,D., Thomas,S.G., Heath,V.L.,
            Herbert,J.M., Bicknell,R., Senis,Y.A., Ashman,L.K.,
            Berditchevski,F., Ouwehand,W.H., Watson,S.P. and Tomlinson,M.G.
  TITLE     Identification of Tspan9 as a novel platelet tetraspanin and the
            collagen receptor GPVI as a component of tetraspanin microdomains
  JOURNAL   Biochem J 417 (1), 391-400 (2009)
   PUBMED   18795891
  REMARK    GeneRIF: Findings suggest a role for Tspan9 in regulating platelet
            function in concert with other platelet tetraspanins and their
            associated proteins.
REFERENCE   8  (bases 1 to 4255)
  AUTHORS   Macaulay,I.C., Tijssen,M.R., Thijssen-Timmer,D.C., Gusnanto,A.,
            Steward,M., Burns,P., Langford,C.F., Ellis,P.D., Dudbridge,F.,
            Zwaginga,J.J., Watkins,N.A., van der Schoot,C.E. and Ouwehand,W.H.
  TITLE     Comparative gene expression profiling of in vitro differentiated
            megakaryocytes and erythroblasts identifies novel activatory and
            inhibitory platelet membrane proteins
  JOURNAL   Blood 109 (8), 3260-3269 (2007)
   PUBMED   17192395
REFERENCE   9  (bases 1 to 4255)
  AUTHORS   Berditchevski,F.
  TITLE     Complexes of tetraspanins with integrins: more than meets the eye
  JOURNAL   J Cell Sci 114 (Pt 23), 4143-4151 (2001)
   PUBMED   11739647
  REMARK    Review article
REFERENCE   10 (bases 1 to 4255)
  AUTHORS   Serru,V., Dessen,P., Boucheix,C. and Rubinstein,E.
  TITLE     Sequence and expression of seven new tetraspans
  JOURNAL   Biochim Biophys Acta 1478 (1), 159-163 (2000)
   PUBMED   10719184
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC125807.9, AC005912.1 and
            AC005865.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001168320.1.
            
            Summary: The protein encoded by this gene is a member of the
            transmembrane 4 superfamily, also known as the tetraspanin family.
            Most of these members are cell-surface proteins that are
            characterized by the presence of four hydrophobic domains. The
            proteins mediate signal transduction events that play a role in the
            regulation of cell development, activation, growth and motility.
            Alternatively spliced transcripts encoding the same protein have
            been identified. [provided by RefSeq, Nov 2009].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853561.6118.1,
                                           SRR14038194.2064389.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                AC125807.9         87379-87453
            76-155              AC005912.1         84446-84525         c
            156-347             AC005912.1         7090-7281           c
            348-422             AC005912.1         6636-6710           c
            423-524             AC005912.1         5219-5320           c
            525-656             AC005912.1         4373-4504           c
            657-740             AC005912.1         3885-3968           c
            741-3397            AC005912.1         1-2657              c
            3398-4255           AC005865.1         45011-45868
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.33-p13.32"
     gene            1..4255
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /note="tetraspanin 9"
                     /db_xref="GeneID:10867"
                     /db_xref="HGNC:HGNC:21640"
                     /db_xref="MIM:613137"
     exon            1..75
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /inference="alignment:Splign:2.1.0"
     exon            76..155
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..812
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /note="transmembrane 4 superfamily member tetraspan NET-5;
                     new EST tetraspan 5; tspan-9"
                     /codon_start=1
                     /product="tetraspanin-9"
                     /protein_id="NP_001161792.1"
                     /db_xref="CCDS:CCDS8520.1"
                     /db_xref="GeneID:10867"
                     /db_xref="HGNC:HGNC:21640"
                     /db_xref="MIM:613137"
                     /translation="MARGCLCCLKYMMFLFNLIFWLCGCGLLGVGIWLSVSQGNFATF
                     SPSFPSLSAANLVIAIGTIVMVTGFLGCLGAIKENKCLLLSFFIVLLVILLAELILLI
                     LFFVYMDKVNENAKKDLKEGLLLYHTENNVGLKNAWNIIQAEMRCCGVTDYTDWYPVL
                     GENTVPDRCCMENSQGCGRNATTPLWRTGCYEKVKMWFDDNKHVLGTVGMCILIMQIL
                     GMAFSMTLFQHIHRTGKKYDA"
     exon            156..347
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /inference="alignment:Splign:2.1.0"
     exon            348..422
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /inference="alignment:Splign:2.1.0"
     exon            423..524
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /inference="alignment:Splign:2.1.0"
     exon            525..656
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /inference="alignment:Splign:2.1.0"
     exon            657..740
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /inference="alignment:Splign:2.1.0"
     exon            741..4255
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4228..4233
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /note="hexamer: AATAAA"
     polyA_site      4255
                     /gene="TSPAN9"
                     /gene_synonym="NET-5; NET5; PP1057"
                     /note="major polyA site"
ORIGIN      
        1 gtttccgctg gcggcggcgg cggcggcggt gccggagcgc gagcagagcg gagaccccca
       61 ggtcctgcgg gcgcgaattt aagaagtgca acatggccag gggctgcctc tgctgcttga
      121 agtacatgat gttcctcttc aatttgatat tctggctctg tggctgtggg ctgctgggag
      181 tgggcatctg gctctccgtg tcccaaggca actttgccac cttctccccc agcttccctt
      241 cgttgtctgc agccaacctg gtcattgcca taggcaccat tgtcatggtg acgggcttcc
      301 tcggctgcct gggggccatc aaggaaaaca agtgcctcct cctcagcttt ttcatcgtcc
      361 tgttggtcat cctcctagca gagctgatct tactcatcct cttctttgtc tacatggaca
      421 aggtgaacga gaacgccaag aaggacctga aggaaggcct gctgctgtac cacaccgaga
      481 acaacgtggg gctgaagaac gcctggaaca tcatccaggc tgagatgcga tgctgtggtg
      541 tcactgacta cacagactgg tacccagtgc tgggggagaa cacggttccc gaccgctgct
      601 gcatggagaa ctcccagggc tgcgggcgca acgccaccac gcctttgtgg agaacgggct
      661 gctatgaaaa ggtgaagatg tggttcgatg acaataagca cgtgctgggc acggtgggga
      721 tgtgcatcct catcatgcag atcctgggca tggccttctc catgaccctc ttccagcaca
      781 tccaccggac tggtaagaag tacgacgcat gagcgggctg gccgggagtg cccaccccgc
      841 cctgctgccc tgtggaggga agaggattga gctttgtgtc acctgcctgc gctctccaga
      901 tatgacccct gcacccaccc cccacagcct gccctacccc acctaccctg cctcagcctc
      961 ggacttctca gtgggtggag tgccagggag gaggaggcac acggagacct ggggctcggg
     1021 gcccctggat tcctgcatct gcatatgcgt atttgccaaa gacgacaggg tgggctgggg
     1081 tgcgctccgg aggaaccccc ggcactgatg ggcttctgcc cctgcccttc ctcacactga
     1141 cactttgtcc ccacatgggg tggggagcag agtgcccgcc ccgtggagat accgccccag
     1201 cgggggctgc gacatccatg gccaccatgg ggcacctggc ggggcggggg tctgccggcc
     1261 tctgggcaag gcccctggag catctcgccc aggcttttta taccttacaa tgtaactttt
     1321 ttattttatt ttactctatg attattcagg aatattatct ctcagataag tttagggtta
     1381 gatttctgat ttgtaacttt ttactgtgtt gatttcttta atggtttgac tttttttccc
     1441 tgagggtgag ggatgggtgg gaagagagga catctgtcca gtctcaatca ggacagacca
     1501 ccgtgcgaca cccaggaggc tctcggatgg ggcgcgcctg cgccctcaga acgtgtggga
     1561 aggagggggc gtggacagga cacgggacct tgccaggcct ggtgtctgag gacaggagcc
     1621 tgggagaggc gggtggagcg tgaagcaggc tggaggtgcc cctgcacggg aggtggcgtt
     1681 tgctaaccaa tcgagctgga aatgccaggg caggggggcc ccacggtgct gtggcagagc
     1741 tagaggggtc cttagacttt tcactgatga gcagttgttg gttttttctt tctcccttcc
     1801 tcccgctctc tgctggcacg cgaggcttcc ccttccaccc catgtgggta ttcccacaac
     1861 aggttctgca caccccagtt atttcacaga cattcctgct agaaactgtc agacaaatac
     1921 ctctctagtt cggatgctgc tcactttccc ccttgcttct ggaaggggaa gcagtcctta
     1981 ggttttgctg tgctgggaca gtggcagggg acccacaggg gtgaggaccc acggtcctcc
     2041 ccgccagcct tgctcagctg tgggttgccc tgctgggaag gagggaatca cgtccacctg
     2101 ggtcccaaga tcttcgcctc cttccctggg gccacggaca tcagcagtgg gttgggtggc
     2161 gattatatca tctgtgatcc caaggagaag aaatacagaa aacccaagag aggtcagact
     2221 ggctcttgtt accggagcca cgggaagaaa gcagccggag tcacgcacgt gcagagctgg
     2281 gcatgggaga gaaacgggct ggggagtgag gccaggagtg ggattcagct gcagcagggc
     2341 gccccctcca aactgcagct ggtctggctt actgttttgc cgttcaaaaa ggtcgcgaat
     2401 ccgtgggact gagcacgggg acctcacccg ctagccagcg tctgctgcac ttgatcaggt
     2461 ggggccttgg tgggcggctg cctttcctat acagtttgtc ttgtcaccct ggtttcccac
     2521 tggggccagg tctcttctcc agcctccacc tgcctgtctg atccaagagc tgagacacgg
     2581 ccacccagca ccagtcactc ctctgttcac cttaagtaac acacaaatcg ggaacaggag
     2641 gacagaaccg ttggcattat caggattcgt gttttgtggg ggtgggagtg gagagtaggg
     2701 tggtcttgtg agttgtgcag ggtgaagacc gcttccctga gacaggggca gtggtgctga
     2761 tggaatgtgg gggaggccca catttgagca aagctgccct gcccttgtcc cctggcctgg
     2821 cttcctggta aggagtttca gccgcctccg caggaacccc caaagtgcag attccggagc
     2881 agacacatcc gggcggagag actcagcaga caagtgctgc agttgcacgg tgggacccgg
     2941 ggcctcgtgc gttttttgct gtgggtgggg tgggtgggtt ggtttatgcc tatcaatgca
     3001 atttttaatt tttgttaata tcaacagcaa aagcctagtg cattgggaga tgtgcaacct
     3061 ccctgaaaat cttttctgtt tctggagtac ttcaggggtg gcctctggcc ccagagcctt
     3121 tgccacagtg ctcccaccag cccccacctc atccgtctgt ttgcagagcc tcatctacag
     3181 gtccccacgc tgccttcttt actcactctg cgcttggccg ttttgttatt tggcttagtc
     3241 tacattgggc ggaagtctgt gtgcacagag tgggtgttcc ttcgagcccc ttccactcag
     3301 agggccacac ccagcgatgc cagtgaaggt ggcacagcct ctcttcagtt tctcctgact
     3361 gtgatctcac tggggtagaa ttcccctgag agaattccct cactcacggc tccctttgcc
     3421 agagtcagtt caatcaggtc tgatgtgagc aatttacaca cttgtctcag aaagtccctc
     3481 agggtttgta gaggactgca ggggggcatc cgctgcagac tcagcctttc tctgcagcca
     3541 tcctgcagtg ggggtgagcg ggcacaggct gagaactgct cttgggtggt ggaagcaggt
     3601 gtcacggtgc aagtctcccc ctgcacccct cccccagctt gagccgtgtc acccccctct
     3661 ccctccagca tgggcctgtg tctcaggctc tctggaaggt ggccctgccc cggaccctct
     3721 tgcaggtgtc ctggtttgac ttggaactag atggccatct ttccaggctt tggtggccca
     3781 agagcagtct gggtggatgg aagtggctgt cccctcctct ccagcccctg cccacccact
     3841 ggtggaggtg ctaactagca gggacgtggc ataggatggg agctgggcgt gaggtgcttg
     3901 gggtccattc tttgtccctc agcttctcag agtccggcca gcccttgtgt tcccgtgccc
     3961 cacactttcc tcctccccac tgcagtgagt caatagtcca gggtggggcc tggcctccct
     4021 gccctgattg gggactcagg aggtgaggcc tggggggctt cctgccccct ccttgcccac
     4081 ctgcctgccc ccgggcagca cgggagggag agcagggtga gcacgcttgt tggtttcaga
     4141 tgcactttct gcttgcattg ccgtatctgt gcgttccttc atcctggtcc tggctttatg
     4201 gaacaccatg tttttagcat gtttttaaat aaaaacggat aaagtgtcaa aagca
//
