LOCUS       NM_004616               1131 bp    mRNA    linear   PRI 12-AUG-2024
DEFINITION  Homo sapiens tetraspanin 8 (TSPAN8), transcript variant 1, mRNA.
ACCESSION   NM_004616
VERSION     NM_004616.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Mrozowska,M., Gornicki,T., Olbromski,M., Partynska,A.I.,
            Dziegiel,P. and Rusak,A.
  TITLE     New insights into the role of tetraspanin 6, 7, and 8 in physiology
            and pathology
  JOURNAL   Cancer Med 13 (14), e7390 (2024)
   PUBMED   39031113
  REMARK    GeneRIF: New insights into the role of tetraspanin 6, 7, and 8 in
            physiology and pathology.
            Review article
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Suwatthanarak,T., Tanjak,P., Chaiboonchoe,A., Acharayothin,O.,
            Thanormjit,K., Chanthercrob,J., Suwatthanarak,T., Niyomchan,A.,
            Tanaka,M., Okochi,M., Pongpaibul,A., Chalermwai,W.V.,
            Trakarnsanga,A., Methasate,A., Pithukpakorn,M. and
            Chinswangwatanakul,V.
  TITLE     Overexpression of TSPAN8 in consensus molecular subtype 3
            colorectal cancer
  JOURNAL   Exp Mol Pathol 137, 104911 (2024)
   PUBMED   38861838
  REMARK    GeneRIF: Overexpression of TSPAN8 in consensus molecular subtype 3
            colorectal cancer.
REFERENCE   3  (bases 1 to 1131)
  AUTHORS   Xie,X. and Wei,D.
  TITLE     miR-378a-5p represses Barrett's esophagus cells proliferation,
            migration and invasion through targeting TSPAN8
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (2), 97-103 (2024)
   PUBMED   38430035
  REMARK    GeneRIF: miR-378a-5p represses Barrett's esophagus cells
            proliferation, migration and invasion through targeting TSPAN8.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1131)
  AUTHORS   Zhang,L., Xu,Y., Cai,E., Zheng,M., Liu,L., Wang,Q. and Li,S.
  TITLE     TSPAN8 regulates EGFR/AKT pathway to enhance metastasis in gastric
            cancer
  JOURNAL   Mol Biol Rep 50 (10), 7955-7965 (2023)
   PUBMED   37535246
  REMARK    GeneRIF: TSPAN8 regulates EGFR/AKT pathway to enhance metastasis in
            gastric cancer.
REFERENCE   5  (bases 1 to 1131)
  AUTHORS   Khatiwada,P., Rimal,U., Han,Z., Malla,M., Zhou,J. and
            Shemshedini,L.
  TITLE     The Transmembrane Protein TM4SF3 Interacts With AR and AR-V7 and is
            Recruited to AR Target Genes
  JOURNAL   Endocrinology 164 (5) (2023)
   PUBMED   36951301
  REMARK    GeneRIF: The Transmembrane Protein TM4SF3 Interacts With AR and
            AR-V7 and is Recruited to AR Target Genes.
REFERENCE   6  (bases 1 to 1131)
  AUTHORS   Berditchevski,F.
  TITLE     Complexes of tetraspanins with integrins: more than meets the eye
  JOURNAL   J Cell Sci 114 (Pt 23), 4143-4151 (2001)
   PUBMED   11739647
  REMARK    Review article
REFERENCE   7  (bases 1 to 1131)
  AUTHORS   Kanetaka,K., Sakamoto,M., Yamamoto,Y., Yamasaki,S., Lanza,F.,
            Kanematsu,T. and Hirohashi,S.
  TITLE     Overexpression of tetraspanin CO-029 in hepatocellular carcinoma
  JOURNAL   J Hepatol 35 (5), 637-642 (2001)
   PUBMED   11690710
REFERENCE   8  (bases 1 to 1131)
  AUTHORS   Serru,V., Le Naour,F., Billard,M., Azorsa,D.O., Lanza,F.,
            Boucheix,C. and Rubinstein,E.
  TITLE     Selective tetraspan-integrin complexes (CD81/alpha4beta1,
            CD151/alpha3beta1, CD151/alpha6beta1) under conditions disrupting
            tetraspan interactions
  JOURNAL   Biochem J 340 (Pt 1) (Pt 1), 103-111 (1999)
   PUBMED   10229664
REFERENCE   9  (bases 1 to 1131)
  AUTHORS   Gwynn,B., Eicher,E.M. and Peters,L.L.
  TITLE     Genetic localization of Cd63, a member of the transmembrane 4
            superfamily, reveals two distinct loci in the mouse genome
  JOURNAL   Genomics 35 (2), 389-391 (1996)
   PUBMED   8661157
REFERENCE   10 (bases 1 to 1131)
  AUTHORS   Szala,S., Kasai,Y., Steplewski,Z., Rodeck,U., Koprowski,H. and
            Linnenbach,A.J.
  TITLE     Molecular cloning of cDNA for the human tumor-associated antigen
            CO-029 and identification of related transmembrane antigens
  JOURNAL   Proc Natl Acad Sci U S A 87 (17), 6833-6837 (1990)
   PUBMED   2395876
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025575.21.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 25, 2018 this sequence version replaced NM_004616.2.
            
            Summary: The protein encoded by this gene is a member of the
            transmembrane 4 superfamily, also known as the tetraspanin family.
            Most of these members are cell-surface proteins that are
            characterized by the presence of four hydrophobic domains. The
            proteins mediate signal transduction events that play a role in the
            regulation of cell development, activation, growth and motility.
            This encoded protein is a cell surface glycoprotein that is known
            to complex with integrins. This gene is expressed in different
            carcinomas. The use of alternate polyadenylation sites has been
            found for this gene. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC005246.1, SRR11853565.7293.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000247829.8/ ENSP00000247829.3
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                AC025575.21        63207-63276
            71-239              AC025575.21        63419-63587
            240-302             AC025575.21        76993-77055
            303-440             AC025575.21        81358-81495
            441-515             AC025575.21        82976-83050
            516-623             AC025575.21        83146-83253
            624-755             AC025575.21        88382-88513
            756-839             AC025575.21        91792-91875
            840-1131            AC025575.21        95819-96110
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q21.1"
     gene            1..1131
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /note="tetraspanin 8"
                     /db_xref="GeneID:7103"
                     /db_xref="HGNC:HGNC:11855"
                     /db_xref="MIM:600769"
     exon            1..70
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /inference="alignment:Splign:2.1.0"
     exon            71..239
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /inference="alignment:Splign:2.1.0"
     CDS             180..893
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /note="transmembrane 4 superfamily member 3;
                     tumor-associated antigen CO-029; tspan-8"
                     /codon_start=1
                     /product="tetraspanin-8"
                     /protein_id="NP_004607.1"
                     /db_xref="CCDS:CCDS8999.1"
                     /db_xref="GeneID:7103"
                     /db_xref="HGNC:HGNC:11855"
                     /db_xref="MIM:600769"
                     /translation="MAGVSACIKYSMFTFNFLFWLCGILILALAIWVRVSNDSQAIFG
                     SEDVGSSSYVAVDILIAVGAIIMILGFLGCCGAIKESRCMLLLFFIGLLLILLLQVAT
                     GILGAVFKSKSDRIVNETLYENTKLLSATGESEKQFQEAIIVFQEEFKCCGLVNGAAD
                     WGNNFQHYPELCACLDKQRPCQSYNGKQVYKETCISFIKDFLAKNLIIVIGISFGLAV
                     IEILGLVFSMVLYCQIGNK"
     exon            240..302
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /inference="alignment:Splign:2.1.0"
     exon            303..440
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /inference="alignment:Splign:2.1.0"
     exon            441..515
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /inference="alignment:Splign:2.1.0"
     exon            516..623
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /inference="alignment:Splign:2.1.0"
     exon            624..755
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /inference="alignment:Splign:2.1.0"
     exon            756..839
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /inference="alignment:Splign:2.1.0"
     exon            840..1131
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1109..1114
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /note="hexamer: AATAAA"
     polyA_site      1131
                     /gene="TSPAN8"
                     /gene_synonym="CO-029; TM4SF3"
                     /note="major polyA site"
ORIGIN      
        1 agtgccccag gagctatgac aagcaaagga acatacttgc ctggagatag cctttgcgat
       61 atttaaatgt ccgtggatac agaaatctct gcaggcaagt tgctccagag catattgcag
      121 gacaagcctg taacgaatag ttaaattcac ggcatctgga ttcctaatcc ttttccgaaa
      181 tggcaggtgt gagtgcctgt ataaaatatt ctatgtttac cttcaacttc ttgttctggc
      241 tatgtggtat cttgatccta gcattagcaa tatgggtacg agtaagcaat gactctcaag
      301 caatttttgg ttctgaagat gtaggctcta gctcctacgt tgctgtggac atattgattg
      361 ctgtaggtgc catcatcatg attctgggct tcctgggatg ctgcggtgct ataaaagaaa
      421 gtcgctgcat gcttctgttg tttttcatag gcttgcttct gatcctgctc ctgcaggtgg
      481 cgacaggtat cctaggagct gttttcaaat ctaagtctga tcgcattgtg aatgaaactc
      541 tctatgaaaa cacaaagctt ttgagcgcca caggggaaag tgaaaaacaa ttccaggaag
      601 ccataattgt gtttcaagaa gagtttaaat gctgcggttt ggtcaatgga gctgctgatt
      661 ggggaaataa ttttcaacac tatcctgaat tatgtgcctg tctagataag cagagaccat
      721 gccaaagcta taatggaaaa caagtttaca aagagacctg tatttctttc ataaaagact
      781 tcttggcaaa aaatttgatt atagttattg gaatatcatt tggactggca gttattgaga
      841 tactgggttt ggtgttttct atggtcctgt attgccagat cgggaacaaa tgaatctgtg
      901 gatgcatcaa cctatcgtca gtcaaacccc tttaaaatgt tgctttggct ttgtaaattt
      961 aaatatgtaa gtgctatata agtcaggagc agctgtcttt ttaaaatgtc tcggctagct
     1021 agaccacaga tatcttctag acatattgaa cacatttaag atttgaggga tataagggaa
     1081 aatgatatga atgtgtattt ttactcaaaa taaaagtaac tgtttacgtt g
//
