LOCUS       NM_001025237            1489 bp    mRNA    linear   PRI 10-JUN-2024
DEFINITION  Homo sapiens tetraspanin 4 (TSPAN4), transcript variant 1, mRNA.
ACCESSION   NM_001025237
VERSION     NM_001025237.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Zheng,Y., Lang,Y., Qi,B. and Li,T.
  TITLE     TSPAN4 and migrasomes in atherosclerosis regression correlated to
            myocardial infarction and pan-cancer progression
  JOURNAL   Cell Adh Migr 17 (1), 14-19 (2023)
   PUBMED   36513632
  REMARK    GeneRIF: TSPAN4 and migrasomes in atherosclerosis regression
            correlated to myocardial infarction and pan-cancer progression.
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Pitsava,G., Feldkamp,M.L., Pankratz,N., Lane,J., Kay,D.M.,
            Conway,K.M., Shaw,G.M., Reefhuis,J., Jenkins,M.M., Almli,L.M.,
            Olshan,A.F., Pangilinan,F., Brody,L.C., Sicko,R.J., Hobbs,C.A.,
            Bamshad,M., McGoldrick,D., Nickerson,D.A., Finnell,R.H.,
            Mullikin,J., Romitti,P.A. and Mills,J.L.
  CONSRTM   University of Washington Center for Mendelian Genomics, NISC
            Comparative Sequencing Program and the National Birth Defects
            Prevention Study
  TITLE     Exome sequencing of child-parent trios with bladder exstrophy:
            Findings in 26 children
  JOURNAL   Am J Med Genet A 185 (10), 3028-3041 (2021)
   PUBMED   34355505
  REMARK    GeneRIF: Exome sequencing of child-parent trios with bladder
            exstrophy: Findings in 26 children.
REFERENCE   3  (bases 1 to 1489)
  AUTHORS   Ma,X., Verweij,E.W.E., Siderius,M., Leurs,R. and Vischer,H.F.
  TITLE     Identification of TSPAN4 as Novel Histamine H4 Receptor Interactor
  JOURNAL   Biomolecules 11 (8), 1127 (2021)
   PUBMED   34439793
  REMARK    GeneRIF: Identification of TSPAN4 as Novel Histamine H4 Receptor
            Interactor.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1489)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1489)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1489)
  AUTHORS   Yauch,R.L., Kazarov,A.R., Desai,B., Lee,R.T. and Hemler,M.E.
  TITLE     Direct extracellular contact between integrin alpha(3)beta(1) and
            TM4SF protein CD151
  JOURNAL   J Biol Chem 275 (13), 9230-9238 (2000)
   PUBMED   10734060
REFERENCE   7  (bases 1 to 1489)
  AUTHORS   Lozahic,S., Christiansen,D., Manie,S., Gerlier,D., Billard,M.,
            Boucheix,C. and Rubinstein,E.
  TITLE     CD46 (membrane cofactor protein) associates with multiple beta1
            integrins and tetraspans
  JOURNAL   Eur J Immunol 30 (3), 900-907 (2000)
   PUBMED   10741407
REFERENCE   8  (bases 1 to 1489)
  AUTHORS   Serru,V., Le Naour,F., Billard,M., Azorsa,D.O., Lanza,F.,
            Boucheix,C. and Rubinstein,E.
  TITLE     Selective tetraspan-integrin complexes (CD81/alpha4beta1,
            CD151/alpha3beta1, CD151/alpha6beta1) under conditions disrupting
            tetraspan interactions
  JOURNAL   Biochem J 340 (Pt 1) (Pt 1), 103-111 (1999)
   PUBMED   10229664
REFERENCE   9  (bases 1 to 1489)
  AUTHORS   Todd,S.C., Doctor,V.S. and Levy,S.
  TITLE     Sequences and expression of six new members of the
            tetraspanin/TM4SF family
  JOURNAL   Biochim Biophys Acta 1399 (1), 101-104 (1998)
   PUBMED   9714763
REFERENCE   10 (bases 1 to 1489)
  AUTHORS   Tachibana,I., Bodorova,J., Berditchevski,F., Zutter,M.M. and
            Hemler,M.E.
  TITLE     NAG-2, a novel transmembrane-4 superfamily (TM4SF) protein that
            complexes with integrins and other TM4SF proteins
  JOURNAL   J Biol Chem 272 (46), 29181-29189 (1997)
   PUBMED   9360996
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP006623.1, BI820547.1,
            BM755435.1, BC000389.2 and BM555783.1.
            
            On Aug 14, 2020 this sequence version replaced NM_001025237.1.
            
            Summary: The protein encoded by this gene is a member of the
            transmembrane 4 superfamily, also known as the tetraspanin family.
            Most of these members are cell-surface proteins that are
            characterized by the presence of four hydrophobic domains. The
            proteins mediate signal transduction events that play a role in the
            regulation of cell development, activation, growth and motility.
            This encoded protein is a cell surface glycoprotein and is similar
            in sequence to its family member CD53 antigen. It is known to
            complex with integrins and other transmembrane 4 superfamily
            proteins. Alternatively spliced transcript variants encoding
            different isoforms have been identified. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2, 3, 4, 5 and 6 encode the same isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14478891.340980.1,
                                           SRR7346977.601693.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                AP006623.1         25631-25662
            33-174              BI820547.1         20-161
            175-448             BM755435.1         93-366
            449-456             BC000389.2         293-300
            457-487             BM555783.1         385-415
            488-1489            BC000389.2         332-1333
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.5"
     gene            1..1489
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /note="tetraspanin 4"
                     /db_xref="GeneID:7106"
                     /db_xref="HGNC:HGNC:11859"
                     /db_xref="MIM:602644"
     exon            1..174
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            175..274
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            275..354
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             292..1008
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /note="isoform a is encoded by transcript variant 1;
                     transmembrane 4 superfamily member 7; tetraspan TM4SF;
                     novel antigen 2"
                     /codon_start=1
                     /product="tetraspanin-4 isoform a"
                     /protein_id="NP_001020408.1"
                     /db_xref="CCDS:CCDS7721.1"
                     /db_xref="GeneID:7106"
                     /db_xref="HGNC:HGNC:11859"
                     /db_xref="MIM:602644"
                     /translation="MARACLQAVKYLMFAFNLLFWLGGCGVLGVGIWLAATQGSFATL
                     SSSFPSLSAANLLIITGAFVMAIGFVGCLGAIKENKCLLLTFFLLLLLVFLLEATIAI
                     LFFAYTDKIDRYAQQDLKKGLHLYGTQGNVGLTNAWSIIQTDFRCCGVSNYTDWFEVY
                     NATRVPDSCCLEFSESCGLHAPGTWWKAPCYETVKVWLQENLLAVGIFGLCTALVQIL
                     GLTFAMTMYCQVVKADTYCA"
     exon            355..546
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            547..621
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            622..723
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            724..855
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            856..939
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1489
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1471..1476
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /note="hexamer: AATAAA"
     polyA_site      1489
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /note="major polyA site"
ORIGIN      
        1 ccatttccca gcagagcccc tgagatctgg agactgcccc agcctcaggc tctgtgcatc
       61 tcccccgagc ccccaccaca tatgtgcgga ggcccctggg ggcccaggcc tgtcctcagt
      121 cccgactggg ctctacctga ggaccctggg gagcctgggc ctggccctcc tcagagcttg
      181 gggcttcctt ggtcgcaccc accacctgcc tgcccactgg tcagccttca gggaccctga
      241 gcaccgcctg gtctctttcc tgtggccagc ccagaactga agcgctgcgg catggcgcgc
      301 gcctgcctcc aggccgtcaa gtacctcatg ttcgccttca acctgctctt ctggctggga
      361 ggctgtggcg tgctgggtgt cggcatctgg ctggccgcca cacaggggag cttcgccacg
      421 ctgtcctctt ccttcccgtc cctgtcggct gccaacttgc tcatcatcac cggcgccttt
      481 gtcatggcca tcggcttcgt gggctgcctg ggtgccatca aggagaacaa gtgcctcctg
      541 ctcactttct tcctgctgct gctgctggtg ttcctgctgg aggccaccat cgccatcctc
      601 ttcttcgcct acacggacaa gattgacagg tatgcccagc aagacctgaa gaaaggcttg
      661 cacctgtacg gcacgcaggg caacgtgggc ctcaccaacg cctggagcat catccagacc
      721 gacttccgct gctgtggcgt ctccaactac actgactggt tcgaggtgta caacgccacg
      781 cgggtacctg actcctgctg cttggagttc agtgagagct gtgggctgca cgcccccggc
      841 acctggtgga aggcgccgtg ctacgagacg gtgaaggtgt ggcttcagga gaacctgctg
      901 gctgtgggca tctttgggct gtgcacggcg ctggtgcaga tcctgggcct gaccttcgcc
      961 atgaccatgt actgccaagt ggtcaaggca gacacctact gcgcgtaggc cgcccaccgc
     1021 ccgcttctct gccaaaagga cgcccacggg gagatggccg cacccacagc tgcctttccc
     1081 accaccagcc tcggtgctct gccccatgct gggaggaggg agggagggac aggtgcctgg
     1141 agcccccgga accctgtttc tggaaggccc tagctcaggt ggcttcaggg cctccggacc
     1201 ccccctggga ggggtggcca cgtgctggct gcggaaccca gggcaggggt gggaggggcc
     1261 tccagcactt tttatattta cgtattctcc aaagcagtgt tcacacggga gccagcctgt
     1321 ggcccccagc ctcctggaaa acaggttggc gctggaggag ccgggtcttg gcatcctgga
     1381 ggtggcccca ctggtcctgg tgctccaggc ggggccgtgg acccctcacc tacattccat
     1441 agtgggcccg tggggctcct ggtgcatctt aataaagtgt gagcagcaa
//
