LOCUS       NM_001025234            1397 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens tetraspanin 4 (TSPAN4), transcript variant 2, mRNA.
ACCESSION   NM_001025234
VERSION     NM_001025234.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Zheng,Y., Lang,Y., Qi,B. and Li,T.
  TITLE     TSPAN4 and migrasomes in atherosclerosis regression correlated to
            myocardial infarction and pan-cancer progression
  JOURNAL   Cell Adh Migr 17 (1), 14-19 (2023)
   PUBMED   36513632
  REMARK    GeneRIF: TSPAN4 and migrasomes in atherosclerosis regression
            correlated to myocardial infarction and pan-cancer progression.
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Pitsava,G., Feldkamp,M.L., Pankratz,N., Lane,J., Kay,D.M.,
            Conway,K.M., Shaw,G.M., Reefhuis,J., Jenkins,M.M., Almli,L.M.,
            Olshan,A.F., Pangilinan,F., Brody,L.C., Sicko,R.J., Hobbs,C.A.,
            Bamshad,M., McGoldrick,D., Nickerson,D.A., Finnell,R.H.,
            Mullikin,J., Romitti,P.A. and Mills,J.L.
  CONSRTM   University of Washington Center for Mendelian Genomics, NISC
            Comparative Sequencing Program and the National Birth Defects
            Prevention Study
  TITLE     Exome sequencing of child-parent trios with bladder exstrophy:
            Findings in 26 children
  JOURNAL   Am J Med Genet A 185 (10), 3028-3041 (2021)
   PUBMED   34355505
  REMARK    GeneRIF: Exome sequencing of child-parent trios with bladder
            exstrophy: Findings in 26 children.
REFERENCE   3  (bases 1 to 1397)
  AUTHORS   Ma,X., Verweij,E.W.E., Siderius,M., Leurs,R. and Vischer,H.F.
  TITLE     Identification of TSPAN4 as Novel Histamine H4 Receptor Interactor
  JOURNAL   Biomolecules 11 (8), 1127 (2021)
   PUBMED   34439793
  REMARK    GeneRIF: Identification of TSPAN4 as Novel Histamine H4 Receptor
            Interactor.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1397)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1397)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1397)
  AUTHORS   Yauch,R.L., Kazarov,A.R., Desai,B., Lee,R.T. and Hemler,M.E.
  TITLE     Direct extracellular contact between integrin alpha(3)beta(1) and
            TM4SF protein CD151
  JOURNAL   J Biol Chem 275 (13), 9230-9238 (2000)
   PUBMED   10734060
REFERENCE   7  (bases 1 to 1397)
  AUTHORS   Lozahic,S., Christiansen,D., Manie,S., Gerlier,D., Billard,M.,
            Boucheix,C. and Rubinstein,E.
  TITLE     CD46 (membrane cofactor protein) associates with multiple beta1
            integrins and tetraspans
  JOURNAL   Eur J Immunol 30 (3), 900-907 (2000)
   PUBMED   10741407
REFERENCE   8  (bases 1 to 1397)
  AUTHORS   Serru,V., Le Naour,F., Billard,M., Azorsa,D.O., Lanza,F.,
            Boucheix,C. and Rubinstein,E.
  TITLE     Selective tetraspan-integrin complexes (CD81/alpha4beta1,
            CD151/alpha3beta1, CD151/alpha6beta1) under conditions disrupting
            tetraspan interactions
  JOURNAL   Biochem J 340 (Pt 1) (Pt 1), 103-111 (1999)
   PUBMED   10229664
REFERENCE   9  (bases 1 to 1397)
  AUTHORS   Todd,S.C., Doctor,V.S. and Levy,S.
  TITLE     Sequences and expression of six new members of the
            tetraspanin/TM4SF family
  JOURNAL   Biochim Biophys Acta 1399 (1), 101-104 (1998)
   PUBMED   9714763
REFERENCE   10 (bases 1 to 1397)
  AUTHORS   Tachibana,I., Bodorova,J., Berditchevski,F., Zutter,M.M. and
            Hemler,M.E.
  TITLE     NAG-2, a novel transmembrane-4 superfamily (TM4SF) protein that
            complexes with integrins and other TM4SF proteins
  JOURNAL   J Biol Chem 272 (46), 29181-29189 (1997)
   PUBMED   9360996
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BQ679002.1, BM755435.1,
            BC000389.2 and BM555783.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001025234.1.
            
            Summary: The protein encoded by this gene is a member of the
            transmembrane 4 superfamily, also known as the tetraspanin family.
            Most of these members are cell-surface proteins that are
            characterized by the presence of four hydrophobic domains. The
            proteins mediate signal transduction events that play a role in the
            regulation of cell development, activation, growth and motility.
            This encoded protein is a cell surface glycoprotein and is similar
            in sequence to its family member CD53 antigen. It is known to
            complex with integrins and other transmembrane 4 superfamily
            proteins. Alternatively spliced transcript variants encoding
            different isoforms have been identified. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Variants 1, 2, 3, 4, 5 and 6 encode the same
            isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC093031.1, SRR14038196.3121198.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                BQ679002.1         30-111
            83-356              BM755435.1         93-366
            357-364             BC000389.2         293-300
            365-395             BM555783.1         385-415
            396-1397            BC000389.2         332-1333
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.5"
     gene            1..1397
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /note="tetraspanin 4"
                     /db_xref="GeneID:7106"
                     /db_xref="HGNC:HGNC:11859"
                     /db_xref="MIM:602644"
     exon            1..82
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            83..182
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            183..262
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..916
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /note="isoform a is encoded by transcript variant 2;
                     transmembrane 4 superfamily member 7; tetraspan TM4SF;
                     novel antigen 2"
                     /codon_start=1
                     /product="tetraspanin-4 isoform a"
                     /protein_id="NP_001020405.1"
                     /db_xref="CCDS:CCDS7721.1"
                     /db_xref="GeneID:7106"
                     /db_xref="HGNC:HGNC:11859"
                     /db_xref="MIM:602644"
                     /translation="MARACLQAVKYLMFAFNLLFWLGGCGVLGVGIWLAATQGSFATL
                     SSSFPSLSAANLLIITGAFVMAIGFVGCLGAIKENKCLLLTFFLLLLLVFLLEATIAI
                     LFFAYTDKIDRYAQQDLKKGLHLYGTQGNVGLTNAWSIIQTDFRCCGVSNYTDWFEVY
                     NATRVPDSCCLEFSESCGLHAPGTWWKAPCYETVKVWLQENLLAVGIFGLCTALVQIL
                     GLTFAMTMYCQVVKADTYCA"
     exon            263..454
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            455..529
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            530..631
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            632..763
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            764..847
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     exon            848..1397
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1379..1384
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /note="hexamer: AATAAA"
     polyA_site      1397
                     /gene="TSPAN4"
                     /gene_synonym="NAG-2; NAG2; TETRASPAN; TM4SF7; TSPAN-4"
                     /note="major polyA site"
ORIGIN      
        1 gaagggcagg acaggggccc tgagtaacgg tggcctgggg tcagagccag gttcagctgc
       61 gaggctgctg gtggctgtgg agagcttggg gcttccttgg tcgcacccac cacctgcctg
      121 cccactggtc agccttcagg gaccctgagc accgcctggt ctctttcctg tggccagccc
      181 agaactgaag cgctgcggca tggcgcgcgc ctgcctccag gccgtcaagt acctcatgtt
      241 cgccttcaac ctgctcttct ggctgggagg ctgtggcgtg ctgggtgtcg gcatctggct
      301 ggccgccaca caggggagct tcgccacgct gtcctcttcc ttcccgtccc tgtcggctgc
      361 caacttgctc atcatcaccg gcgcctttgt catggccatc ggcttcgtgg gctgcctggg
      421 tgccatcaag gagaacaagt gcctcctgct cactttcttc ctgctgctgc tgctggtgtt
      481 cctgctggag gccaccatcg ccatcctctt cttcgcctac acggacaaga ttgacaggta
      541 tgcccagcaa gacctgaaga aaggcttgca cctgtacggc acgcagggca acgtgggcct
      601 caccaacgcc tggagcatca tccagaccga cttccgctgc tgtggcgtct ccaactacac
      661 tgactggttc gaggtgtaca acgccacgcg ggtacctgac tcctgctgct tggagttcag
      721 tgagagctgt gggctgcacg cccccggcac ctggtggaag gcgccgtgct acgagacggt
      781 gaaggtgtgg cttcaggaga acctgctggc tgtgggcatc tttgggctgt gcacggcgct
      841 ggtgcagatc ctgggcctga ccttcgccat gaccatgtac tgccaagtgg tcaaggcaga
      901 cacctactgc gcgtaggccg cccaccgccc gcttctctgc caaaaggacg cccacgggga
      961 gatggccgca cccacagctg cctttcccac caccagcctc ggtgctctgc cccatgctgg
     1021 gaggagggag ggagggacag gtgcctggag cccccggaac cctgtttctg gaaggcccta
     1081 gctcaggtgg cttcagggcc tccggacccc ccctgggagg ggtggccacg tgctggctgc
     1141 ggaacccagg gcaggggtgg gaggggcctc cagcactttt tatatttacg tattctccaa
     1201 agcagtgttc acacgggagc cagcctgtgg cccccagcct cctggaaaac aggttggcgc
     1261 tggaggagcc gggtcttggc atcctggagg tggccccact ggtcctggtg ctccaggcgg
     1321 ggccgtggac ccctcaccta cattccatag tgggcccgtg gggctcctgg tgcatcttaa
     1381 taaagtgtga gcagcaa
//
