LOCUS       NM_005725               3216 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens tetraspanin 2 (TSPAN2), transcript variant 1, mRNA.
ACCESSION   NM_005725
VERSION     NM_005725.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Reynolds,J.L. and Mahajan,S.D.
  TITLE     Transmigration of Tetraspanin 2 (Tspan2) siRNA Via Microglia
            Derived Exosomes across the Blood Brain Barrier Modifies the
            Production of Immune Mediators by Microglia Cells
  JOURNAL   J Neuroimmune Pharmacol 15 (3), 554-563 (2020)
   PUBMED   31823250
  REMARK    GeneRIF: Transmigration of Tetraspanin 2 (Tspan2) siRNA Via
            Microglia Derived Exosomes across the Blood Brain Barrier Modifies
            the Production of Immune Mediators by Microglia Cells.
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 3216)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3216)
  AUTHORS   Richard,M.A., Huan,T., Ligthart,S., Gondalia,R., Jhun,M.A.,
            Brody,J.A., Irvin,M.R., Marioni,R., Shen,J., Tsai,P.C.,
            Montasser,M.E., Jia,Y., Syme,C., Salfati,E.L., Boerwinkle,E.,
            Guan,W., Mosley,T.H. Jr., Bressler,J., Morrison,A.C., Liu,C.,
            Mendelson,M.M., Uitterlinden,A.G., van Meurs,J.B., Franco,O.H.,
            Zhang,G., Li,Y., Stewart,J.D., Bis,J.C., Psaty,B.M., Chen,Y.I.,
            Kardia,S.L.R., Zhao,W., Turner,S.T., Absher,D., Aslibekyan,S.,
            Starr,J.M., McRae,A.F., Hou,L., Just,A.C., Schwartz,J.D.,
            Vokonas,P.S., Menni,C., Spector,T.D., Shuldiner,A., Damcott,C.M.,
            Rotter,J.I., Palmas,W., Liu,Y., Paus,T., Horvath,S.,
            O'Connell,J.R., Guo,X., Pausova,Z., Assimes,T.L., Sotoodehnia,N.,
            Smith,J.A., Arnett,D.K., Deary,I.J., Baccarelli,A.A., Bell,J.T.,
            Whitsel,E., Dehghan,A., Levy,D. and Fornage,M.
  CONSRTM   BIOS Consortium
  TITLE     DNA Methylation Analysis Identifies Loci for Blood Pressure
            Regulation
  JOURNAL   Am J Hum Genet 101 (6), 888-902 (2017)
   PUBMED   29198723
  REMARK    GeneRIF: the authors have identified TSPAN2 as a candidate gene for
            Blood Pressure Regulation that is regulated by heritable DNA
            methylation
REFERENCE   5  (bases 1 to 3216)
  AUTHORS   Yaseen,I.H., Monk,P.N. and Partridge,L.J.
  TITLE     Tspan2: a tetraspanin protein involved in oligodendrogenesis and
            cancer metastasis
  JOURNAL   Biochem Soc Trans 45 (2), 465-475 (2017)
   PUBMED   28408487
  REMARK    GeneRIF: Studies suggest that TSPAN2 may contribute to the early
            stages of the oligodendrocyte differentiation into myelin-forming
            glia. Furthermore, in human lung cancer, Tspan2 could be involved
            in the progression of the tumour metastasis by modulating cancer
            cell motility and invasion functions. [review]
            Review article
REFERENCE   6  (bases 1 to 3216)
  AUTHORS   Esserlind,A.L., Christensen,A.F., Le,H., Kirchmann,M., Hauge,A.W.,
            Toyserkani,N.M., Hansen,T., Grarup,N., Werge,T., Steinberg,S.,
            Bettella,F., Stefansson,H. and Olesen,J.
  TITLE     Replication and meta-analysis of common variants identifies a
            genome-wide significant locus in migraine
  JOURNAL   Eur J Neurol 20 (5), 765-772 (2013)
   PUBMED   23294458
  REMARK    GeneRIF: the C-allele of rs2078371 near TSPAN-2 also reached
            genome-wide significance for association with migraine.
REFERENCE   7  (bases 1 to 3216)
  AUTHORS   Davila,S., Froeling,F.E., Tan,A., Bonnard,C., Boland,G.J.,
            Snippe,H., Hibberd,M.L. and Seielstad,M.
  TITLE     New genetic associations detected in a host response study to
            hepatitis B vaccine
  JOURNAL   Genes Immun 11 (3), 232-238 (2010)
   PUBMED   20237496
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 3216)
  AUTHORS   Hemler,M.E.
  TITLE     Specific tetraspanin functions
  JOURNAL   J Cell Biol 155 (7), 1103-1107 (2001)
   PUBMED   11756464
  REMARK    Review article
REFERENCE   9  (bases 1 to 3216)
  AUTHORS   Berditchevski,F.
  TITLE     Complexes of tetraspanins with integrins: more than meets the eye
  JOURNAL   J Cell Sci 114 (Pt 23), 4143-4151 (2001)
   PUBMED   11739647
  REMARK    Review article
REFERENCE   10 (bases 1 to 3216)
  AUTHORS   Todd,S.C., Doctor,V.S. and Levy,S.
  TITLE     Sequences and expression of six new members of the
            tetraspanin/TM4SF family
  JOURNAL   Biochim Biophys Acta 1399 (1), 101-104 (1998)
   PUBMED   9714763
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL109660.14, CB993308.1,
            AK315385.1, BG697236.1, AK022144.1, AU117697.1, AA724831.1,
            AW051326.1 and AW015874.1.
            
            On Nov 23, 2018 this sequence version replaced NM_005725.5.
            
            Summary: The protein encoded by this gene is a member of the
            transmembrane 4 superfamily, also known as the tetraspanin family.
            Most of these members are cell-surface proteins that are
            characterized by the presence of four hydrophobic domains. The
            proteins mediate signal transduction events that play a role in the
            regulation of cell development, activation, growth and motility.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Apr 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.107984.1,
                                           SRR1803612.128369.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369516.7/ ENSP00000358529.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AL109660.14        87726-87728         c
            4-312               CB993308.1         27-335
            313-737             AK315385.1         270-694
            738-1142            BG697236.1         317-721
            1143-2308           AK022144.1         329-1494
            2309-2309           AU117697.1         201-201
            2310-2849           AK022144.1         1496-2035
            2850-3139           AA724831.1         77-366              c
            3140-3140           AW051326.1         81-81               c
            3141-3215           AA724831.1         2-76                c
            3216-3216           AW015874.1         15-15               c
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p13.2"
     gene            1..3216
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /note="tetraspanin 2"
                     /db_xref="GeneID:10100"
                     /db_xref="HGNC:HGNC:20659"
                     /db_xref="MIM:613133"
     exon            1..140
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..737
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tetraspan 2; tetraspan TM4SF; new EST tetraspan 3;
                     tetraspan NET-3"
                     /codon_start=1
                     /product="tetraspanin-2 isoform 1"
                     /protein_id="NP_005716.2"
                     /db_xref="CCDS:CCDS881.1"
                     /db_xref="GeneID:10100"
                     /db_xref="HGNC:HGNC:20659"
                     /db_xref="MIM:613133"
                     /translation="MGRFRGGLRCIKYLLLGFNLLFWLAGSAVIAFGLWFRFGGAIKE
                     LSSEDKSPEYFYVGLYVLVGAGALMMAVGFFGCCGAMRESQCVLGSFFTCLLVIFAAE
                     VTTGVFAFIGKGVAIRHVQTMYEEAYNDYLKDRGKGNGTLITFHSTFQCCGKESSEQV
                     QPTCPKELLGHKNCIDEIETIISVKLQLIGIVGIGIAGLTIFGMIFSMVLCCAIRNSR
                     DVI"
     exon            141..243
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /inference="alignment:Splign:2.1.0"
     exon            244..341
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /inference="alignment:Splign:2.1.0"
     exon            342..416
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /inference="alignment:Splign:2.1.0"
     exon            417..515
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /inference="alignment:Splign:2.1.0"
     exon            516..587
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /inference="alignment:Splign:2.1.0"
     exon            588..671
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /inference="alignment:Splign:2.1.0"
     exon            672..3216
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      983..988
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /note="hexamer: AATGAA"
     polyA_site      1004
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /note="major polyA site"
     regulatory      1991..1996
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /note="hexamer: AATAAA"
     polyA_site      2009
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
     regulatory      3194..3199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /note="hexamer: ATTAAA"
     polyA_site      3216
                     /gene="TSPAN2"
                     /gene_synonym="NET3; TSN2; TSPAN-2"
                     /note="major polyA site"
ORIGIN      
        1 gccggagcct gccgctcccc gcgctcgtag cgcgggcctg gggactgggg atcccgccgc
       61 cgggccgcag catggggcgc ttccgcgggg gcctgcggtg catcaagtac ctgctgcttg
      121 gcttcaacct gctcttctgg ctggctggat cggccgtcat tgcttttgga ctatggtttc
      181 ggttcggagg tgccataaag gagttatcat cagaggacaa gtccccagag tatttctatg
      241 tggggctgta tgttctggtt ggagccgggg ccctgatgat ggccgtgggg ttcttcgggt
      301 gctgcggagc catgcgggag tcgcaatgtg tgcttggatc attttttacc tgcctcctgg
      361 tgatatttgc tgctgaagta accactggag tatttgcttt tataggcaag ggggtagcta
      421 tccgacatgt tcagaccatg tatgaagagg cttacaatga ttaccttaaa gacaggggaa
      481 aaggcaatgg gacactcatc accttccact caacatttca gtgctgtgga aaagaaagct
      541 ccgaacaggt ccaacctaca tgcccaaagg agcttctagg acacaagaat tgcatcgatg
      601 aaattgagac cataatcagt gttaagctcc agctcattgg aattgtcggt attggaattg
      661 caggtctgac gatctttggc atgatattca gcatggtcct ctgctgtgcg atacgaaact
      721 cacgagatgt gatatgaagc tacttctaca tgaaaattgc aatctaaagc tttcatacca
      781 aatgtcacag gagctgtctc ccagctcatt tttaacactg aaatgacatt aggatctaaa
      841 ataatttgct gtcaattgta catttgcatg agtacgtatg tttggctcat tactggttta
      901 ccccttgagt gaatgcctgt ttatgatgac tgagagcata ttcatgtgtg atctgcgtgt
      961 ttctggaata tgctttatac gtaatgaaat ctgtttgctg ggaattcctg attcttgtta
     1021 tataagaaga acaacctatt tcgctcccag aaaaaaaaga tcaaagagct ttcagaaact
     1081 ttgagaactt ggctatttag aaaaagtgat aatgggtcag tttctcagac tgtagccatt
     1141 gaaaattaga tgcagagaat tcagagattt cttcttaatg gaagtaataa gctgtaagaa
     1201 ttgagagatc acaatggagt gttaaaactg actgtgtcta agttgggtgt aagggtttcc
     1261 tgggtttttt tatatacatg ctctccccag aatacagtaa accacagttt tagaactaaa
     1321 cacatctgta aaactaaata tagcatggaa aatccaattt gaataagtca tgctttccta
     1381 gaatttaaaa ataaaaaagt cttcctctgg aaagagaagt cacacagaca atcatgtgcc
     1441 ctataaaagt gagtgtttat aggactaaaa aacttttaac aactttttaa ggaaatattt
     1501 ttgttcttat acaaaaacat gtaaatattg ctttattact ttcattttct gaccctgctg
     1561 taaactactg caaccctcac atcctcaaag ggacttttat gtcaaactct tctgtttctc
     1621 caaatataag gaaaaaagac taaagcaaga gatctggcag ttgaaaattg tgggaaagag
     1681 aatttgtatg ggcactgtat ctatgaaata cctcataact tacgtttaca tgttttccta
     1741 actttttgta tttttcttgt atagccacct agagaattct tcatagatta agaactacag
     1801 ttttcaccac ttaacataag taaaacaaag tccttcataa tttaaccatt agcatctttg
     1861 gccaaaccaa aataaagaaa agcatcttct cctagttgtg tgtgggcaac agaaacaagt
     1921 taaggaaaca aaaatactta tatatacaca gaacaaaaat aatgttcttt ttatgcaaat
     1981 cccctgtgaa aataaaattt tcaatgtttt tttggtctta acgcttcatt ttaacttagt
     2041 aaacccaaat ttaggattta cattggcaaa tttttcagtg atttttaacg tgttacattt
     2101 agtaaaccat agatttttta aaaactacat tttgtaaggc cacacataag tgaaaatacg
     2161 gaaggctttg gaaaccccca gatgaacttc tatacatgtg aaaacacaag tctttgagtg
     2221 tgcactaaaa gtgtcacact cccaaaaaac tcacaaaaca ttttgctaaa aagatatgac
     2281 tgcttgaaat agtctctctc ccatggagaa ccacatttct ctttctgcca tacttgctac
     2341 tgctactgat tttggaggaa atttttctag aagaacaaag gtcatatttg aggcaagcta
     2401 aaaacttaat tatacatggc tactttactg tgtctgtagc taaagaaaaa ttattttaca
     2461 aaaattactt catgaaatat agtttgtacc ccagtagttg agaaatgtga ccttttaggc
     2521 aaagaacagg cagtccatct ctcttagctt tagtttatct ctctgcctga agacaggaaa
     2581 caggcccaag tgcatactcg ggttctttcc aactcagaat catctctgat tccacaaaag
     2641 tgagtttagt ttcctatctg aattaacaac tttaaaggag actataatag ttaaaagtgg
     2701 aagaatagaa ataaataaat ttaaaatgaa attaattaaa gtagaagaga agggttctgt
     2761 tccatgtacg attaatgtgc cccttttgga gataagcttt ccaaaatatg cgtatgagta
     2821 aaattagaga attccaccac taagcaacaa tagattttat atgtgtgtgt ggatatatat
     2881 acacacacac acagaaagat gaatatatat atatatatat ctgtatagac acacatataa
     2941 tatatataga tatataatcc ctatatatct tgaatctaca aatatcttta tatgtatata
     3001 atttgaatat atacatgtat gtatatacag atgtgtgtgt atatatatat atacatacac
     3061 atatacataa attttcagct atttctgttc ccctgaacta atttgagatt agacccaaac
     3121 atgcttttaa atgtctcatg ccaaggcact gaattaagac ttcttaatgt tgaaatattt
     3181 taagtgattt catattaaat tttctatata ctgaaa
//
