LOCUS       NM_001282510             927 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens tetraspanin 16 (TSPAN16), transcript variant 3, mRNA.
ACCESSION   NM_001282510 XM_005259852
VERSION     NM_001282510.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 927)
  AUTHORS   Zhang,W., Zhang,X. and Qu,S.
  TITLE     Cysteine Scanning Mutagenesis of TM4b-4c Loop of Glutamate
            Transporter EAAT1 Reveals Three Conformationally Sensitive Residues
  JOURNAL   Mol Pharmacol 94 (1), 713-721 (2018)
   PUBMED   29654220
  REMARK    GeneRIF: To probe the structural role of the TM4b-4c loop of EAAT1
            (Rattus norvegicus), each of the 57 amino acid residues was mutated
            to cysteine.
REFERENCE   3  (bases 1 to 927)
  AUTHORS   Weissglas-Volkov,D., Aguilar-Salinas,C.A., Nikkola,E., Deere,K.A.,
            Cruz-Bautista,I., Arellano-Campos,O., Munoz-Hernandez,L.L.,
            Gomez-Munguia,L., Ordonez-Sanchez,M.L., Reddy,P.M., Lusis,A.J.,
            Matikainen,N., Taskinen,M.R., Riba,L., Cantor,R.M.,
            Sinsheimer,J.S., Tusie-Luna,T. and Pajukanta,P.
  TITLE     Genomic study in Mexicans identifies a new locus for triglycerides
            and refines European lipid loci
  JOURNAL   J Med Genet 50 (5), 298-308 (2013)
   PUBMED   23505323
REFERENCE   4  (bases 1 to 927)
  AUTHORS   Puls,K.L., Ni,J., Liu,D., Morahan,G. and Wright,M.D.
  TITLE     The molecular characterisation of a novel tetraspanin protein,
            TM4-B(1)
  JOURNAL   Biochim Biophys Acta 1447 (1), 93-99 (1999)
   PUBMED   10500248
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB457828.1, BM560276.1,
            AW169944.1 and AI688554.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001282510.1.
            
            Summary: The protein encoded by this gene is a member of the
            transmembrane 4 superfamily, also known as the tetraspanin family.
            Most of these members are cell-surface proteins that are
            characterized by the presence of four hydrophobic domains. The
            proteins mediate signal transduction events that play a role in the
            regulation of cell development, activation, growth and motility.
            This encoded protein might couple to signal transduction pathways
            and possibly modulate cellular activation and adhesion in
            haemopoietic and neural tissue. Several transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Sep 2013].
            
            Transcript Variant: This variant (3) differs in the 3' UTR and
            coding sequence and lacks an alternate in-frame exon compared to
            variant 1. The resulting isoform (3) has a shorter and distinct
            C-terminus and lacks an alternate internal segment compared to
            isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.365266.1,
                                           SRR5189667.57862.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                DB457828.1         12-74
            64-405              BM560276.1         72-413
            406-715             BM560276.1         489-798
            716-923             AW169944.1         10-217              c
            924-927             AI688554.1         23-26               c
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.2"
     gene            1..927
                     /gene="TSPAN16"
                     /gene_synonym="TM-8; TM4-B; TM4SF16"
                     /note="tetraspanin 16"
                     /db_xref="GeneID:26526"
                     /db_xref="HGNC:HGNC:30725"
                     /db_xref="MIM:617580"
     exon            1..207
                     /gene="TSPAN16"
                     /gene_synonym="TM-8; TM4-B; TM4SF16"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..798
                     /gene="TSPAN16"
                     /gene_synonym="TM-8; TM4-B; TM4SF16"
                     /note="isoform 3 is encoded by transcript variant 3;
                     transmembrane 4 superfamily member 16; tetraspanin TM4-B;
                     tspan-16"
                     /codon_start=1
                     /product="tetraspanin-16 isoform 3"
                     /protein_id="NP_001269439.1"
                     /db_xref="CCDS:CCDS62550.1"
                     /db_xref="GeneID:26526"
                     /db_xref="HGNC:HGNC:30725"
                     /db_xref="MIM:617580"
                     /translation="MAEIHTPYSSLKKLLSLLNGFVAVSGIILVGLGIGGKCGGASLT
                     NVLGLSSAYLLHVGNLCLVMGCITVLLGCAGWYGATKESRGTLLFVGDVALEHTFVTL
                     RKNYRGYNEPDDYSTQWNLVMEKLKCCGVNNYTDFSGSSFEMTTGHTYPRSCCKSIGS
                     VSCDGRDVSPNVIHQKGCFHKLLKITKTQSFTLSGSSLGAAVIQLPGILATLLLFIKL
                     G"
     exon            208..405
                     /gene="TSPAN16"
                     /gene_synonym="TM-8; TM4-B; TM4SF16"
                     /inference="alignment:Splign:2.1.0"
     exon            406..513
                     /gene="TSPAN16"
                     /gene_synonym="TM-8; TM4-B; TM4SF16"
                     /inference="alignment:Splign:2.1.0"
     exon            514..666
                     /gene="TSPAN16"
                     /gene_synonym="TM-8; TM4-B; TM4SF16"
                     /inference="alignment:Splign:2.1.0"
     exon            667..750
                     /gene="TSPAN16"
                     /gene_synonym="TM-8; TM4-B; TM4SF16"
                     /inference="alignment:Splign:2.1.0"
     exon            751..927
                     /gene="TSPAN16"
                     /gene_synonym="TM-8; TM4-B; TM4SF16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      906..911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSPAN16"
                     /gene_synonym="TM-8; TM4-B; TM4SF16"
                     /note="hexamer: AATAAA"
     polyA_site      927
                     /gene="TSPAN16"
                     /gene_synonym="TM-8; TM4-B; TM4SF16"
                     /note="major polyA site"
ORIGIN      
        1 aggcagccag gacacagagg ggcagagcaa gtcagcattg gcgccccttc ctcagatccc
       61 tatcatcttg ggaaacagta gcccagaggt tcaggaagat gttaacttaa atgttcaggg
      121 tgccccagtc tgttcagcat ggctgaaatc cacactccgt attcttcctt gaagaaactg
      181 ttatctttac tcaatggctt cgtggctgtg tctggcatca tcctagttgg cctgggcatt
      241 ggtggtaaat gtggaggggc ctctctgacg aatgtcctcg ggctgtcctc cgcatacctc
      301 cttcacgttg gcaacctgtg cctggtgatg ggatgcatca cggtactgct tggctgtgcc
      361 gggtggtatg gagcgactaa agagagcaga ggcacgctct tgtttgttgg agatgtggcc
      421 ttggaacaca ccttcgtgac cctgaggaag aattacagag gttacaacga gccagacgac
      481 tattctacac agtggaactt ggtcatggag aagctaaagt gctgtggggt gaataactac
      541 acagattttt ctggctcttc cttcgaaatg acaacgggcc acacctaccc caggagttgc
      601 tgtaaatcca tcggaagtgt gtcctgtgac ggacgcgatg tgtctccaaa cgtcatccac
      661 cagaagggct gtttccataa actcctaaaa atcaccaaga ctcagagctt caccctgagt
      721 gggagctctc tgggagctgc agtgatacag ttgccaggaa ttcttgccac tttgctgctg
      781 tttatcaagc tgggctgaca cccaggcctg gagaagatga gacacctggg cccatctggc
      841 tgctggagat tcagtctcag ttttatttct ctgtggcact cactgcttct ggaggggaga
      901 ctgttaataa aagatttggg aaccccc
//
