LOCUS       NM_015516               2585 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU),
            transcript variant 1, mRNA.
ACCESSION   NM_015516
VERSION     NM_015516.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Li,Y., Jin,L., Yan,J., Huang,Y., Zhang,H., Zhang,R. and Hu,C.
  TITLE     Tsukushi and TSKU genotype in obesity and related metabolic
            disorders
  JOURNAL   J Endocrinol Invest 44 (12), 2645-2654 (2021)
   PUBMED   33860453
  REMARK    GeneRIF: Tsukushi and TSKU genotype in obesity and related
            metabolic disorders.
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Deng,X., Li,Y., Guo,C., Zhao,Z. and Yuan,G.
  TITLE     Novel roles of Tsukushi in signaling pathways and multiple disease
            processes
  JOURNAL   Biofactors 47 (4), 512-521 (2021)
   PUBMED   33759220
  REMARK    GeneRIF: Novel roles of Tsukushi in signaling pathways and multiple
            disease processes.
            Review article
REFERENCE   3  (bases 1 to 2585)
  AUTHORS   Liu,D., Zhang,P., Wei,X., Deng,Y., Liu,W., Guo,D., Liu,J., Xu,B.,
            Huang,C., Huang,J., Lin,J., Liu,S., Xue,Y. and Zhang,H.
  TITLE     Elevated Serum Tsukushi Levels in Patients With Hyperthyroidism
  JOURNAL   Front Endocrinol (Lausanne) 11, 580097 (2020)
   PUBMED   33117292
  REMARK    GeneRIF: Elevated Serum Tsukushi Levels in Patients With
            Hyperthyroidism.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2585)
  AUTHORS   Grander,C., Jaschke,N., Enrich,B., Grabherr,F., Mayr,L.,
            Schwarzler,J., Effenberger,M., Adolph,T.E. and Tilg,H.
  TITLE     Gastric banding-associated weight loss diminishes hepatic Tsukushi
            expression
  JOURNAL   Cytokine 133, 155114 (2020)
   PUBMED   32442908
  REMARK    GeneRIF: Gastric banding-associated weight loss diminishes hepatic
            Tsukushi expression.
REFERENCE   5  (bases 1 to 2585)
  AUTHORS   Mouchiroud,M., Camire,E., Aldow,M., Caron,A., Jubinville,E.,
            Turcotte,L., Kaci,I., Beaulieu,M.J., Roy,C., Labbe,S.M.,
            Varin,T.V., Gelinas,Y., Lamothe,J., Trottier,J., Mitchell,P.L.,
            Guenard,F., Festuccia,W.T., Joubert,P., Rose,C.F., Karvellas,C.J.,
            Barbier,O., Morissette,M.C., Marette,A. and Laplante,M.
  TITLE     The hepatokine Tsukushi is released in response to NAFLD and
            impacts cholesterol homeostasis
  JOURNAL   JCI Insight 4 (15), 129492 (2019)
   PUBMED   31391339
  REMARK    GeneRIF: The hepatokine Tsukushi is released in response to NAFLD
            and impacts cholesterol homeostasis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2585)
  AUTHORS   Henke,A., Grace,O.C., Ashley,G.R., Stewart,G.D., Riddick,A.C.,
            Yeun,H., O'Donnell,M., Anderson,R.A. and Thomson,A.A.
  TITLE     Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and
            Tsukushi in developing prostate and decreased levels of decorin in
            prostate cancer
  JOURNAL   PLoS One 7 (8), e42516 (2012)
   PUBMED   22880013
  REMARK    GeneRIF: The spatio-temporal expression of Decorin, Semaphorin6D,
            SPARC, Spry-1 and Tsukushi in developing prostate is described and
            observed similar mesenchymal expression patterns in rat and human.
REFERENCE   7  (bases 1 to 2585)
  AUTHORS   Rakhshandehroo,M., Hooiveld,G., Muller,M. and Kersten,S.
  TITLE     Comparative analysis of gene regulation by the transcription factor
            PPARalpha between mouse and human
  JOURNAL   PLoS One 4 (8), e6796 (2009)
   PUBMED   19710929
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2585)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   9  (bases 1 to 2585)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   10 (bases 1 to 2585)
  AUTHORS   Charpentier,A.H., Bednarek,A.K., Daniel,R.L., Hawkins,K.A.,
            Laflin,K.J., Gaddis,S., MacLeod,M.C. and Aldaz,C.M.
  TITLE     Effects of estrogen on global gene expression: identification of
            novel targets of estrogen action
  JOURNAL   Cancer Res 60 (21), 5977-5983 (2000)
   PUBMED   11085516
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK091475.1 and BC020975.1.
            
            On Nov 22, 2018 this sequence version replaced NM_015516.3.
            
            Transcript Variant: This variant (1) differs in the 5' UTR and
            coding sequence compared to variant 3. The resulting isoform (b) is
            shorter at the N-terminus compared to isoform a. Variants 1, 2, 4,
            and 5 all encode isoform b.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358317.1, AK075476.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000333090.5/ ENSP00000332668.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AK091475.1         29-35
            8-2585              BC020975.1         1-2578
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.5"
     gene            1..2585
                     /gene="TSKU"
                     /gene_synonym="E2IG4; LRRC54; TSK"
                     /note="tsukushi, small leucine rich proteoglycan"
                     /db_xref="GeneID:25987"
                     /db_xref="HGNC:HGNC:28850"
                     /db_xref="MIM:608015"
     exon            1..49
                     /gene="TSKU"
                     /gene_synonym="E2IG4; LRRC54; TSK"
                     /inference="alignment:Splign:2.1.0"
     exon            50..2585
                     /gene="TSKU"
                     /gene_synonym="E2IG4; LRRC54; TSK"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..1119
                     /gene="TSKU"
                     /gene_synonym="E2IG4; LRRC54; TSK"
                     /note="isoform b precursor is encoded by transcript
                     variant 1; tsukushi homolog; tsukushin; leucine rich
                     repeat containing 54; E2-induced gene 4 protein;
                     leucine-rich repeat-containing protein 54; tsukushi small
                     leucine rich proteoglycan homolog"
                     /codon_start=1
                     /product="tsukushi isoform b precursor"
                     /protein_id="NP_056331.2"
                     /db_xref="CCDS:CCDS8246.1"
                     /db_xref="GeneID:25987"
                     /db_xref="HGNC:HGNC:28850"
                     /db_xref="MIM:608015"
                     /translation="MPWPLLLLLAVSGAQTTRPCFPGCQCEVETFGLFDSFSLTRVDC
                     SGLGPHIMPVPIPLDTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPT
                     AFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRAL
                     HVDLSHNLIHRLVPHPTRAGLPAPTIQSLNLAWNRLHAVPNLRDLPLRYLSLDGNPLA
                     VIGPGAFAGLGGLTHLSLASLQRLPELAPSGFRELPGLQVLDLSGNPKLNWAGAEVFS
                     GLSSLQELDLSGTNLVPLPEALLLHLPALQSVSVGQDVRCRRLVREGTYPRRPGSSPK
                     VALHCVDTRDSAARGPTIL"
     regulatory      2566..2571
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSKU"
                     /gene_synonym="E2IG4; LRRC54; TSK"
                     /note="hexamer: AATAAA"
     polyA_site      2585
                     /gene="TSKU"
                     /gene_synonym="E2IG4; LRRC54; TSK"
                     /note="major polyA site"
ORIGIN      
        1 cgggccgccg gggtgagcgt gccgaggcgg ctgtggcgca ggcttccagc ccccaccatg
       61 ccgtggcccc tgctgctgct gctggccgtg agtggggccc agacaacccg gccatgcttc
      121 cccgggtgcc aatgcgaggt ggagaccttc ggccttttcg acagcttcag cctgactcgg
      181 gtggattgta gcggcctggg cccccacatc atgccggtgc ccatccctct ggacacagcc
      241 cacttggacc tgtcctccaa ccggctggag atggtgaatg agtcggtgtt ggcggggccg
      301 ggctacacga cgttggctgg cctggatctc agccacaacc tgctcaccag catctcaccc
      361 actgccttct cccgccttcg ctacctggag tcgcttgacc tcagccacaa tggcctgaca
      421 gccctgccag ccgagagctt caccagctca cccctgagcg acgtgaacct tagccacaac
      481 cagctccggg aggtctcagt gtctgccttc acgacgcaca gtcagggccg ggcactacac
      541 gtggacctct cccacaacct cattcaccgc ctcgtgcccc accccacgag ggccggcctg
      601 cctgcgccca ccattcagag cctgaacctg gcctggaacc ggctccatgc cgtgcccaac
      661 ctccgagact tgcccctgcg ctacctgagc ctggatggga accctctagc tgtcattggt
      721 ccgggtgcct tcgcggggct gggaggcctt acacacctgt ctctggccag cctgcagagg
      781 ctccctgagc tggcgcccag tggcttccgt gagctaccgg gcctgcaggt cctggacctg
      841 tcgggcaacc ccaagcttaa ctgggcagga gctgaggtgt tttcaggcct gagctccctg
      901 caggagctgg acctttcggg caccaacctg gtgcccctgc ctgaggcgct gctcctccac
      961 ctcccggcac tgcagagcgt cagcgtgggc caggatgtgc ggtgccggcg cctggtgcgg
     1021 gagggcacct acccccggag gcctggctcc agccccaagg tggccctgca ctgcgtagac
     1081 acccgggatt ctgctgccag gggccccacc atcttgtgac aaatggtgtg gcccagggcc
     1141 acataacaga ctgctgtcct gggctgcctc aggtcccgag taacttatgt tcaatgtgcc
     1201 aacaccagtg gggagcccgc aggcctatgt ggcagcgtca ccacaggagt tgtgggccta
     1261 ggagaggctt tggacctggg agccacacct aggagcaaag tctcacccct ttgtctacgt
     1321 tgcttcccca aaccatgagc agagggactt cgatgccaaa ccagactcgg gtcccctcct
     1381 gcttcccttc cccacttatc ccccaagtgc cttccctcat gcctgggccg gcctgacccg
     1441 caatgggcag agggtgggtg ggaccccctg ctgcagggca gagttcaggt ccactgggct
     1501 gagtgtcccc ttgggcccat ggcccagtca ctcaggggcg agtttctttt ctaacatagc
     1561 cctttctttg ccatgaggcc atgaggcccg cttcatcctt ttctatttcc ctagaacctt
     1621 aatggtagaa ggaattgcaa agaatcaagt ccacccttct catgtgacag atggggaaac
     1681 tgaggccttg agaaggaaaa aggctaatct aagttcctgc gggcagtggc atgactggag
     1741 cacagcctcc tgcctcccag cccggaccca atgcactttc ttgtctcctc taataagccc
     1801 caccctcccc gcctgggctc cccttgctgc ccttgcctgt tccccattag cacaggagta
     1861 gcagcagcag gacaggcaag agcctcacaa gtgggactct gggcctctga ccagctgtgc
     1921 ggcatgggct aagtcactct gcccttcgga gcctctggaa gcttagggca cattggttcc
     1981 agcctagcca gtttctcacc ctgggttggg gtcccccagc atccagactg gaaacctacc
     2041 cattttcccc tgagcatcct ctagatgctg ccccaaggag ttgctgcagt tctggagcct
     2101 catctggctg ggatctccaa ggggcctcct ggattcagtc cccactggcc ctgagcacga
     2161 cagcccttct taccctccca ggaatgccgt gaaaggagac aaggtctgcc cgacccatgt
     2221 ctatgctcta cccccagggt agcatctcag cttccgaacc ctgggctgtt tccttagtct
     2281 tcattttata aaagttgttg cctttttaac ggagtgtcac tttcaaccgg cctcccctac
     2341 ccctgctggc cggggatgga gacatgtcat ttgtaaaagc agaaaaaggt tgcatttgtt
     2401 cacttttgta atattgtcct gggcctgtgt tggggtgttg ggggaagctg ggcatcagtg
     2461 gccacatggg catcaggggc tggccccaca gagaccccac agggcagtga gctctgtctt
     2521 cccccacctg cctagcccat catctatcta accggtcctt gatttaataa acactataaa
     2581 aagtt
//
