LOCUS       NM_021733               1854 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens testis specific serine kinase substrate (TSKS), mRNA.
ACCESSION   NM_021733
VERSION     NM_021733.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Shetty,J., Sinville,R., Shumilin,I.A., Minor,W., Zhang,J.,
            Hawkinson,J.E., Georg,G.I., Flickinger,C.J. and Herr,J.C.
  TITLE     Recombinant production of enzymatically active male contraceptive
            drug target hTSSK2 - Localization of the TSKS domain phosphorylated
            by TSSK2
  JOURNAL   Protein Expr Purif 121, 88-96 (2016)
   PUBMED   26777341
  REMARK    GeneRIF: n vitro phosphorylation experiments carried out with TSKS
            (isoform 1) fragments revealed particularly strong phosphorylation
            of a recombinant N-terminal region representing aa 1-150 of TSKS,
            indicating that the N-terminus of human TSKS is phosphorylated by
            human TSSK2.
REFERENCE   3  (bases 1 to 1854)
  AUTHORS   Hendrickx,A., Beullens,M., Ceulemans,H., Den Abt,T., Van Eynde,A.,
            Nicolaescu,E., Lesage,B. and Bollen,M.
  TITLE     Docking motif-guided mapping of the interactome of protein
            phosphatase-1
  JOURNAL   Chem Biol 16 (4), 365-371 (2009)
   PUBMED   19389623
REFERENCE   4  (bases 1 to 1854)
  AUTHORS   Xu,B., Hao,Z., Jha,K.N., Zhang,Z., Urekar,C., Digilio,L.,
            Pulido,S., Strauss,J.F. 3rd, Flickinger,C.J. and Herr,J.C.
  TITLE     Targeted deletion of Tssk1 and 2 causes male infertility due to
            haploinsufficiency
  JOURNAL   Dev Biol 319 (2), 211-222 (2008)
   PUBMED   18533145
REFERENCE   5  (bases 1 to 1854)
  AUTHORS   Xu,B., Hao,Z., Jha,K.N., Zhang,Z., Urekar,C., Digilio,L.,
            Pulido,S., Strauss,J.F. 3rd, Flickinger,C.J. and Herr,J.C.
  TITLE     TSKS concentrates in spermatid centrioles during flagellogenesis
  JOURNAL   Dev Biol 319 (2), 201-210 (2008)
   PUBMED   18495105
REFERENCE   6  (bases 1 to 1854)
  AUTHORS   Wu,C., Ma,M.H., Brown,K.R., Geisler,M., Li,L., Tzeng,E., Jia,C.Y.,
            Jurisica,I. and Li,S.S.
  TITLE     Systematic identification of SH3 domain-mediated human
            protein-protein interactions by peptide array target screening
  JOURNAL   Proteomics 7 (11), 1775-1785 (2007)
   PUBMED   17474147
REFERENCE   7  (bases 1 to 1854)
  AUTHORS   Hao,Z., Jha,K.N., Kim,Y.H., Vemuganti,S., Westbrook,V.A.,
            Chertihin,O., Markgraf,K., Flickinger,C.J., Coppola,M., Herr,J.C.
            and Visconti,P.E.
  TITLE     Expression analysis of the human testis-specific serine/threonine
            kinase (TSSK) homologues. A TSSK member is present in the
            equatorial segment of human sperm
  JOURNAL   Mol Hum Reprod 10 (6), 433-444 (2004)
   PUBMED   15044604
REFERENCE   8  (bases 1 to 1854)
  AUTHORS   Scorilas,A., Yousef,G.M., Jung,K., Rajpert-De Meyts,E., Carsten,S.
            and Diamandis,E.P.
  TITLE     Identification and characterization of a novel human
            testis-specific kinase substrate gene which is downregulated in
            testicular tumors
  JOURNAL   Biochem Biophys Res Commun 285 (2), 400-408 (2001)
   PUBMED   11444856
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011495.8.
            
            On Nov 26, 2018 this sequence version replaced NM_021733.1.
            
            Summary: This gene may play a role in testicular physiology,
            spermatogenesis or spermiogenesis. Expression of the encoded
            protein is highest in the testis and down-regulated in testicular
            cancer. The gene is localized to the region 19q13.3 among the
            related RAS viral oncogene homolog (RRAS) and interferon regulatory
            factor 3 (IRF3) genes, which are both involved in tumorigenesis
            pathways and progression. [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058862.1, ERR279875.3295.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000246801.8/ ENSP00000246801.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-229               AC011495.8         188888-189116       c
            230-458             AC011495.8         187814-188042       c
            459-554             AC011495.8         174184-174279       c
            555-638             AC011495.8         173895-173978       c
            639-722             AC011495.8         173199-173282       c
            723-1051            AC011495.8         172280-172608       c
            1052-1246           AC011495.8         171012-171206       c
            1247-1420           AC011495.8         170041-170214       c
            1421-1556           AC011495.8         167695-167830       c
            1557-1681           AC011495.8         165869-165993       c
            1682-1854           AC011495.8         165570-165742       c
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..1854
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /note="testis specific serine kinase substrate"
                     /db_xref="GeneID:60385"
                     /db_xref="HGNC:HGNC:30719"
                     /db_xref="MIM:608253"
     exon            1..229
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..1838
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /note="testis-specific kinase substrate; testis specific
                     serine/threonine kinase substrate; STK22 substrate 1;
                     protein phosphatase 1, regulatory subunit 161"
                     /codon_start=1
                     /product="testis-specific serine kinase substrate"
                     /protein_id="NP_068379.1"
                     /db_xref="CCDS:CCDS12780.1"
                     /db_xref="GeneID:60385"
                     /db_xref="HGNC:HGNC:30719"
                     /db_xref="MIM:608253"
                     /translation="MASVVVKTIWQSKEIHEAGDTPTGVESCSQLVPEAPRRVTSRAK
                     GIPKKKKAVSFHGVEPQMSHQPMHWCLNLKRSSACTNVSLLNLAAMEPTDSTGTDSTV
                     EDLSGQLTLAGPPASPTLPWDPDDADITEILSGVNSGLVRAKDSITSLKEKTNRVNQH
                     VQSLQSECSVLSENLERRRQEAEELEGYCIQLKENCWKVTRSVEDAEIKTNVLKQNSA
                     LLEEKLRYLQQQLQDETPRRQEAELQEPEEKQEPEEKQEPEEKQKPEAGLSWNSLGPA
                     ATSQGCPGPPGSPDKPSRPHGLVPAGWGMGPRAGEGPYVSEQELQKLFTGIEELRREV
                     SSLTARWHQEEGAVQEALRLLGGLGGRVDGFLGQWERAQREQAQTARDLQELRGRADE
                     LCTMVERSAVSVASLRSELEGLGPLKPILEEFGRQFQNSRRGPDLSMNLDRSHQGNCA
                     RCASQGSQLSTESLQQLLDRALTSLVDEVKQRGLTPACPSCQRLHKKILELERQALAK
                     HVRAEALSSTLRLAQDEALRAKNLLLTDKMKPEEKMATLDHLHLKMCSLHDHLSNLPL
                     EGSTGTMGGGSSAGTPPKQGGSAPEQ"
     exon            230..458
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /inference="alignment:Splign:2.1.0"
     exon            459..554
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /inference="alignment:Splign:2.1.0"
     exon            555..638
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /inference="alignment:Splign:2.1.0"
     exon            639..722
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /inference="alignment:Splign:2.1.0"
     exon            723..1051
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1246
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1420
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1556
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..1681
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1854
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1834..1839
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /note="hexamer: AATAAA"
     polyA_site      1854
                     /gene="TSKS"
                     /gene_synonym="PPP1R161; STK22S1; TSKS1; TSSKS"
                     /note="major polyA site"
ORIGIN      
        1 acagtaggta ggaagtctca gaggaaggag cccctccctg ggagccagac ccccacacca
       61 tggcgagcgt ggtggtgaag acgatctggc agtccaaaga gatccatgag gccggggaca
      121 cccccacggg ggtggagagc tgctcccagc tagtcccaga ggctccccgg agggtgacca
      181 gccgggccaa ggggatcccg aagaaaaaga aggccgtgtc gttccacggg gtggagcccc
      241 agatgtccca tcagcccatg cactggtgcc tgaacctcaa acggtcctcg gcctgcacca
      301 acgtgtcact gctcaacctg gccgccatgg agcccactga ctccacgggg acagactcca
      361 cagtggaaga cctcagcggc caactcacac tggctgggcc ccctgcctcc cctaccctac
      421 cctgggatcc ggatgacgca gacatcacgg aaatcctgag tggggtcaac agtggattgg
      481 tccgcgccaa agactccatc accagcttga aggaaaagac caaccgggtt aaccagcacg
      541 tgcagtctct gcagagcgag tgttctgtgc tgagcgagaa tctggagaga aggcggcaag
      601 aggcagaaga gttggagggg tactgcattc aactcaagga gaactgctgg aaggtgaccc
      661 ggtctgtgga agatgctgaa atcaaaacca acgtcttgaa gcagaattct gccctgctgg
      721 aggagaagct gcgctacctc cagcagcagc tgcaggatga gacgccgcga cggcaggagg
      781 ccgagctgca ggagccggag gagaagcagg agccggagga gaagcaggag ccggaggaga
      841 agcagaagcc ggaggctggc ctctcctgga acagcctggg ccccgccgcc acgtcccagg
      901 gctgccccgg cccgccaggg agtcccgaca aaccctcgcg gccacacggc ctggtccccg
      961 caggctgggg aatggggcct cgggctggcg agggccccta cgtgagcgag caggaattgc
     1021 agaagctgtt caccggcatc gaagagctga ggagagaggt gtcctcactg accgcccggt
     1081 ggcatcagga ggagggggcg gtgcaggaag ccctgcggct gctcgggggc ctgggcggca
     1141 gggtcgacgg cttcctaggc cagtgggagc gggcacagcg cgaacaggca cagacggcgc
     1201 gggacttgca ggagctgcga ggtcgggcgg atgagctgtg caccatggtg gagcggtcag
     1261 cagtgtctgt ggcttcactg aggagcgaac tggaggggct gggcccactg aaacccattc
     1321 tggaggagtt cgggcggcaa tttcagaact ctcgaagagg gcctgacctc tccatgaacc
     1381 tggatcggtc ccaccaaggc aactgtgccc gctgtgccag ccaggggtcg cagttgtcta
     1441 cggagtccct gcagcagctg ctggaccgag cactgacctc actagtggac gaggtgaagc
     1501 agaggggcct gactcctgcc tgtcccagct gtcagaggct acacaagaag attctggagc
     1561 tggagcgcca ggccttagcc aaacacgtca gggcagaggc cctgagctcc acccttcggc
     1621 tggcccaaga cgaggccctg cgggccaaga acctactgct gacagacaag atgaagccag
     1681 aggagaagat ggccactctg gaccatctac acttgaagat gtgctccctc cacgatcatc
     1741 tcagcaacct gccacttgag gggtccacgg gaacaatggg gggaggcagc agtgcaggaa
     1801 cccccccaaa acaggggggc tcagcccctg aacaataaat ggcctctcat gcta
//
