LOCUS       NM_173485              12236 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens teashirt zinc finger homeobox 2 (TSHZ2), transcript
            variant 1, mRNA.
ACCESSION   NM_173485 NM_018692
VERSION     NM_173485.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12236)
  AUTHORS   Uribe,M.L., Dahlhoff,M., Batra,R.N., Nataraj,N.B., Haga,Y.,
            Drago-Garcia,D., Marrocco,I., Sekar,A., Ghosh,S., Vaknin,I.,
            Lebon,S., Kramarski,L., Tsutsumi,Y., Choi,I., Rueda,O.M., Caldas,C.
            and Yarden,Y.
  TITLE     TSHZ2 is an EGF-regulated tumor suppressor that binds to the
            cytokinesis regulator PRC1 and inhibits metastasis
  JOURNAL   Sci Signal 14 (688) (2021)
   PUBMED   34158398
  REMARK    GeneRIF: TSHZ2 is an EGF-regulated tumor suppressor that binds to
            the cytokinesis regulator PRC1 and inhibits metastasis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12236)
  AUTHORS   Zhao,S., Guo,X., Mizutani,K.I., Yamaguchi,R., Yamada,S., Gu,C. and
            Uramoto,H.
  TITLE     Overexpression of Teashirt Homolog 2 suppresses cell proliferation
            and predicts the favorable survival of Lung Adenocarcinoma
  JOURNAL   Int J Med Sci 18 (9), 1980-1989 (2021)
   PUBMED   33850468
  REMARK    GeneRIF: Overexpression of Teashirt Homolog 2 suppresses cell
            proliferation and predicts the favorable survival of Lung
            Adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 12236)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 12236)
  AUTHORS   Riku,M., Inaguma,S., Ito,H., Tsunoda,T., Ikeda,H. and Kasai,K.
  TITLE     Down-regulation of the zinc-finger homeobox protein TSHZ2 releases
            GLI1 from the nuclear repressor complex to restore its
            transcriptional activity during mammary tumorigenesis
  JOURNAL   Oncotarget 7 (5), 5690-5701 (2016)
   PUBMED   26744317
  REMARK    GeneRIF: Down-regulation of TSHZ2 is crucial for mammary
            tumorigenesis via the activation of GLI1.
REFERENCE   5  (bases 1 to 12236)
  AUTHORS   Dowling,J.K., Becker,C.E., Bourke,N.M., Corr,S.C., Connolly,D.J.,
            Quinn,S.R., Pandolfi,P.P., Mansell,A. and O'Neill,L.A.J.
  TITLE     Promyelocytic leukemia protein interacts with the
            apoptosis-associated speck-like protein to limit inflammasome
            activation
  JOURNAL   J Biol Chem 289 (10), 6429-6437 (2014)
   PUBMED   24407287
REFERENCE   6  (bases 1 to 12236)
  AUTHORS   Jenkins,D., Caubit,X., Dimovski,A., Matevska,N., Lye,C.M.,
            Cabuk,F., Gucev,Z., Tasic,V., Fasano,L. and Woolf,A.S.
  TITLE     Analysis of TSHZ2 and TSHZ3 genes in congenital pelvi-ureteric
            junction obstruction
  JOURNAL   Nephrol Dial Transplant 25 (1), 54-60 (2010)
   PUBMED   19745106
  REMARK    GeneRIF: Mutations in TSHZ2 and TSHZ3 are not a major cause of
            pelvi-ureteric junction obstruction, at least in Albanian and
            Macedonian populations.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 12236)
  AUTHORS   Vaquerizas,J.M., Kummerfeld,S.K., Teichmann,S.A. and Luscombe,N.M.
  TITLE     A census of human transcription factors: function, expression and
            evolution
  JOURNAL   Nat Rev Genet 10 (4), 252-263 (2009)
   PUBMED   19274049
  REMARK    Review article
REFERENCE   8  (bases 1 to 12236)
  AUTHORS   Gocke,C.B., Yu,H. and Kang,J.
  TITLE     Systematic identification and analysis of mammalian small
            ubiquitin-like modifier substrates
  JOURNAL   J Biol Chem 280 (6), 5004-5012 (2005)
   PUBMED   15561718
REFERENCE   9  (bases 1 to 12236)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   10 (bases 1 to 12236)
  AUTHORS   Collins,C., Rommens,J.M., Kowbel,D., Godfrey,T., Tanner,M.,
            Hwang,S.I., Polikoff,D., Nonet,G., Cochran,J., Myambo,K., Jay,K.E.,
            Froula,J., Cloutier,T., Kuo,W.L., Yaswen,P., Dairkee,S.,
            Giovanola,J., Hutchinson,G.B., Isola,J., Kallioniemi,O.P.,
            Palazzolo,M., Martin,C., Ericsson,C., Pinkel,D., Albertson,D.,
            Li,W.B. and Gray,J.W.
  TITLE     Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2
            and overexpressed in breast carcinoma
  JOURNAL   Proc Natl Acad Sci U S A 95 (15), 8703-8708 (1998)
   PUBMED   9671742
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121902.13, BX640770.1,
            BX276189.1, AL354993.24 and BQ574274.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_173485.5.
            
            Summary: This gene is a member of the teashirt C2H2-type
            zinc-finger protein family of transcription factors. This gene
            encodes a protein with five C2H2-type zinc fingers, a homeobox
            DNA-binding domain and a coiled-coil domain. This nuclear protein
            is predicted to act as a transcriptional repressor. This gene is
            thought to play a role in the development and progression of breast
            and other types of cancer. Alternative splicing results in multiple
            transcript variants encoding different isoforms. [provided by
            RefSeq, Dec 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX640770.1, AK091206.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371497.10/ ENSP00000360552.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                AL121902.13        123464-123512
            50-995              BX640770.1         1-946
            996-3683            BX276189.1         38594-41281
            3684-4178           BX640770.1         3635-4129
            4179-11752          AL354993.24        82345-89918
            11753-12236         BQ574274.1         18-501              c
FEATURES             Location/Qualifiers
     source          1..12236
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.2"
     gene            1..12236
                     /gene="TSHZ2"
                     /gene_synonym="C20orf17; OVC10-2; TSH2; ZABC2; ZNF218"
                     /note="teashirt zinc finger homeobox 2"
                     /db_xref="GeneID:128553"
                     /db_xref="HGNC:HGNC:13010"
                     /db_xref="MIM:614118"
     exon            1..976
                     /gene="TSHZ2"
                     /gene_synonym="C20orf17; OVC10-2; TSH2; ZABC2; ZNF218"
                     /inference="alignment:Splign:2.1.0"
     CDS             937..4041
                     /gene="TSHZ2"
                     /gene_synonym="C20orf17; OVC10-2; TSH2; ZABC2; ZNF218"
                     /note="isoform 1 is encoded by transcript variant 1;
                     teashirt family zinc finger 2; teashirt homolog 2;
                     serologically defined colon cancer antigen 33 like; zinc
                     finger protein 218; cell growth-inhibiting protein 7;
                     ovarian cancer-related protein 10-2"
                     /codon_start=1
                     /product="teashirt homolog 2 isoform 1"
                     /protein_id="NP_775756.3"
                     /db_xref="CCDS:CCDS33490.1"
                     /db_xref="GeneID:128553"
                     /db_xref="HGNC:HGNC:13010"
                     /db_xref="MIM:614118"
                     /translation="MPRRKQQAPKRAAGYAQEEQLKEEEEIKEEEEEEDSGSVAQLQG
                     GNDTGTDEELETGPEQKGCFSYQNSPGSHLSNQDAENESLLSDASDQVSDIKSVCGRD
                     ASDKKAHTHVRLPNEAHNCMDKMTAVYANILSDSYWSGLGLGFKLSNSERRNCDTRNG
                     SNKSDFDWHQDALSKSLQQNLPSRSVSKPSLFSSVQLYRQSSKMCGTVFTGASRFRCR
                     QCSAAYDTLVELTVHMNETGHYQDDNRKKDKLRPTSYSKPRKRAFQDMDKEDAQKVLK
                     CMFCGDSFDSLQDLSVHMIKTKHYQKVPLKEPVPTISSKMVTPAKKRVFDVNRPCSPD
                     STTGSFADSFSSQKNANLQLSSNNRYGYQNGASYTWQFEACKSQILKCMECGSSHDTL
                     QQLTTHMMVTGHFLKVTSSASKKGKQLVLDPLAVEKMQSLSEAPNSDSLAPKPSSNSA
                     SDCTASTTELKKESKKERPEETSKDEKVVKSEDYEDPLQKPLDPTIKYQYLREEDLED
                     GSKGGGDILKSLENTVTTAINKAQNGAPSWSAYPSIHAAYQLSEGTKPPLPMGSQVLQ
                     IRPNLTNKLRPIAPKWKVMPLVSMPTHLAPYTQVKKESEDKDEAVKECGKESPHEEAS
                     SFSHSEGDSFRKSETPPEAKKTELGPLKEEEKLMKEGSEKEKPQPLEPTSALSNGCAL
                     ANHAPALPCINPLSALQSVLNNHLGKATEPLRSPSCSSPSSSTISMFHKSNLNVMDKP
                     VLSPASTRSASVSRRYLFENSDQPIDLTKSKSKKAESSQAQSCMSPPQKHALSDIADM
                     VKVLPKATTPKPASSSRVPPMKLEMDVRRFEDVSSEVSTLHKRKGRQSNWNPQHLLIL
                     QAQFASSLFQTSEGKYLLSDLGPQERMQISKFTGLSMTTISHWLANVKYQLRKTGGTK
                     FLKNMDKGHPIFYCSDCASQFRTPSTYISHLESHLGFQMKDMTRLSVDQQSKVEQEIS
                     RVSSAQRSPETIAAEEDTDSKFKCKLCCRTFVSKHAVKLHLSKTHSKSPEHHSQFVTD
                     VDEE"
     exon            977..4049
                     /gene="TSHZ2"
                     /gene_synonym="C20orf17; OVC10-2; TSH2; ZABC2; ZNF218"
                     /inference="alignment:Splign:2.1.0"
     exon            4050..12236
                     /gene="TSHZ2"
                     /gene_synonym="C20orf17; OVC10-2; TSH2; ZABC2; ZNF218"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6512..6517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSHZ2"
                     /gene_synonym="C20orf17; OVC10-2; TSH2; ZABC2; ZNF218"
                     /note="hexamer: AATAAA"
     polyA_site      6537
                     /gene="TSHZ2"
                     /gene_synonym="C20orf17; OVC10-2; TSH2; ZABC2; ZNF218"
                     /note="major polyA site"
     regulatory      7824..7829
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSHZ2"
                     /gene_synonym="C20orf17; OVC10-2; TSH2; ZABC2; ZNF218"
                     /note="hexamer: AGTAAA"
     polyA_site      7849
                     /gene="TSHZ2"
                     /gene_synonym="C20orf17; OVC10-2; TSH2; ZABC2; ZNF218"
                     /note="major polyA site"
     regulatory      12206..12211
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSHZ2"
                     /gene_synonym="C20orf17; OVC10-2; TSH2; ZABC2; ZNF218"
                     /note="hexamer: AATAAA"
     polyA_site      12236
                     /gene="TSHZ2"
                     /gene_synonym="C20orf17; OVC10-2; TSH2; ZABC2; ZNF218"
                     /note="major polyA site"
ORIGIN      
        1 agtttgttca gcgggatcga tgtttgctgg catcgcctcg ccctgtcttg tgtgtgtgtg
       61 cgagggtgtg tgtgtgtgtt tgtgtgtgtg tgcatatgtg gggggtgtga gtgtgtgtgt
      121 gcgaggaagc gggggtgcgt gcgcgtgtga gtgcgtgtgt gagtgtctgt gtgtgtgtct
      181 gtgtgtgtgt gtgagtgagt gaattccaga ttttctgtct ttccaaaacc cgctcctgtc
      241 ctctcgcata tcactcacag acggggatct gacagcagcc acaaacctac agtgagtgat
      301 cgctctcccc ccggcacgaa tccgccatag agatcggcga ggaggaggag gaggaggagg
      361 aagaaaagaa ggaggaggtg gaggaggagg tggaggagga ggaggaggag ggaaagagga
      421 gaaggaagaa gaagaaaaag aagaaaccca ctaccttccc aggattgcct tttttttttc
      481 cttatcttta cgcgcgagtg tgcctgtggc gcgtgtgcgc ccctcgtccc ttccatccga
      541 acccgggctt ggatgtttaa taaagaaatc aagtgtctca acagtcacca aaaaaaaaaa
      601 aaaccgcaaa aacaaaacca aaaaaattcc aaaagcaaaa acaaaaaaga gagaggaaaa
      661 aaaattcaaa ataaacaaac aaacaaacaa ggcagaacca acctctactt caaagcagcc
      721 ggcacaagcc acccgtgtct gccacccaga gaggggggtc tctggcccgt ggtggaggag
      781 ttgcaggggg gatcgtcagg gggacagagg ccgagtgacg tcctaggagc caccgggcaa
      841 gaggcggagg agacccagag aggccagaga gacagcgggc cccagcgcgc ggctcggggc
      901 tggggcgcca gaagtgggac tggagcgaag tagaggatgc cgaggagaaa acagcaggca
      961 cccaagcggg cggcaggcta cgcccaggag gaacagctga aagaagagga ggaaataaaa
     1021 gaagaggagg aggaggagga cagcggttca gtagctcaac tgcagggtgg caatgacaca
     1081 gggacggacg aggagctaga aacgggccca gagcaaaaag gctgcttcag ctaccagaac
     1141 tctccaggaa gtcatttgtc caatcaggat gccgagaacg agtctctgct gagtgacgcc
     1201 agtgatcagg tgtcggacat caagagtgtc tgcggcagag atgcctcaga caagaaagca
     1261 cacactcacg tcaggcttcc aaacgaagca cacaattgca tggataaaat gaccgctgtc
     1321 tacgccaaca tcctgtcgga ttcctactgg tcaggcctgg gccttggctt caagctgtcc
     1381 aatagtgaga ggaggaactg tgacacccga aacggcagca acaagagtga ttttgattgg
     1441 caccaagacg ctctgtccaa aagcctgcag cagaacttgc cttctcggtc cgtctcgaaa
     1501 cccagcctgt tcagctcggt gcagttgtac cgacagagca gcaagatgtg cgggactgtg
     1561 ttcacagggg ccagcagatt ccgatgccga cagtgcagcg cggcctatga caccctagtc
     1621 gagctgactg tgcacatgaa tgaaacgggc cactatcaag atgacaaccg caaaaaggac
     1681 aagctcagac ccacgagcta ttcaaagccc aggaaaaggg ctttccagga tatggacaaa
     1741 gaggatgctc aaaaggttct gaaatgtatg ttttgtggcg actcctttga ttccctccaa
     1801 gatttgagcg tccacatgat taaaacaaaa cattaccaaa aagtgccttt gaaggagcca
     1861 gtcccaacca tttcctcgaa aatggtcacc ccggctaaga aacgcgtttt tgatgtcaat
     1921 cggccgtgtt cccccgattc aaccacagga tcttttgcag attctttttc ttctcagaag
     1981 aacgccaact tgcagttgtc ctccaacaac cgctatggct accaaaatgg agccagctac
     2041 acctggcagt ttgaggcctg caagtcccag atcttaaagt gcatggagtg tgggagctcc
     2101 catgacacct tgcagcagct caccacccac atgatggtca caggtcactt tctcaaggtc
     2161 accagctctg cctccaagaa agggaagcag ctggtattag acccgttagc agtggagaaa
     2221 atgcagtcgt tgtctgaggc cccaaacagt gattctctgg ctcccaagcc atccagtaac
     2281 tcagcatcag attgtacagc ctctacaact gagttaaaga aagagagtaa aaaagaaagg
     2341 ccagaggaaa ccagcaagga tgagaaagtc gtgaaaagcg aggactatga agatcctcta
     2401 caaaaacctt tagaccctac aatcaaatat caatacctaa gggaggaaga cttggaagat
     2461 ggctcaaagg gtggagggga cattttgaaa tctttggaaa atactgtcac cacagccatc
     2521 aacaaagccc aaaacggggc ccccagctgg agtgcctacc ccagcatcca cgcagcctac
     2581 cagctgtctg agggcaccaa gccgcctttg cctatgggat cccaggtact gcagatccgg
     2641 cctaatctca ccaacaagct gaggcccatt gcaccaaagt ggaaagtgat gccactggtt
     2701 tctatgccca cacacctggc cccttacact caagtcaaga aagagtcaga agacaaagat
     2761 gaagcggtga aggagtgtgg gaaagaaagt ccccacgaag aggcctcatc tttcagccac
     2821 agtgagggcg attctttccg caaaagtgaa acacctccag aagccaaaaa gaccgagctg
     2881 ggtcccctga aggaggagga gaagctgatg aaagagggca gcgagaagga gaaaccccag
     2941 cccctggagc ccacatctgc tctgagcaat gggtgcgccc tcgccaacca cgccccggcc
     3001 ctgccatgca tcaacccact cagcgccctg cagtccgtcc tgaacaatca cttgggcaaa
     3061 gccacggagc ccttgcgctc accttcctgc tccagcccaa gttcaagcac aatttccatg
     3121 ttccacaagt cgaatctcaa tgtcatggac aagccggtct tgagtcctgc ctccacaagg
     3181 tcagccagcg tgtccaggcg ctacctgttt gagaacagcg atcagcccat tgacctgacc
     3241 aagtccaaaa gcaagaaagc cgagtcctcg caagcacaat cttgtatgtc cccacctcag
     3301 aagcacgctc tgtctgacat cgccgacatg gtcaaagtcc tccccaaagc caccacccca
     3361 aagccagcct cctcctccag ggtccccccc atgaagctgg aaatggatgt caggcgcttt
     3421 gaggatgtct ccagtgaagt ctcaactttg cataaaagaa aaggccggca gtccaactgg
     3481 aatcctcagc atcttctgat tctacaagcc cagtttgcct cgagcctctt ccagacatca
     3541 gagggcaaat acctgctgtc tgatctgggc ccacaagagc gtatgcaaat ctctaagttt
     3601 acgggactct caatgaccac tatcagtcac tggctggcca acgtcaagta ccagcttagg
     3661 aaaacgggcg ggacaaaatt tctgaaaaac atggacaaag gccaccccat cttttattgc
     3721 agtgactgtg cctcccagtt cagaacccct tctacctaca tcagtcactt agaatctcac
     3781 ctgggtttcc aaatgaagga catgacccgc ttgtcagtgg accagcaaag caaggtggag
     3841 caagagatct cccgggtatc gtcggctcag aggtctccag aaacaatagc tgccgaagag
     3901 gacacagact ctaaattcaa gtgtaagttg tgctgtcgga catttgtgag caaacatgcg
     3961 gtaaaactcc acctaagcaa aacgcacagc aagtcacccg aacaccattc acagtttgta
     4021 acagacgtgg atgaagaata gctctgcagg acgaatgcct tagtttccac tttccagcct
     4081 ggatcccctc acactgaacc cttcttcgtt gcaccatcct gcttctgaca ttgaactcat
     4141 tgaactcctc ctgacaccct ggctctgaga agactgccaa aaaaaaaaaa aaaaaaaaat
     4201 caccccagcc atttctcttc atcctcacta acaatttggt aatgaagtat tgatttccac
     4261 ttctctgctt atgggcggta ttagattttc attgataaat tgcaatgggg ctgtctcgtc
     4321 tccacagtac ccttttcact gtcacaagaa aacaaagtgc caccgaagaa aagtaatgac
     4381 tgagagcatt gatgtactta ttttgtcagt ttgtaacagg aaagtggggg ggagtctaag
     4441 tcttcatagt ctaatgtcca agtgggttgc actagatgta gacacttgga ggcttacttt
     4501 tcatggtaat gtccatttcc tatttataac ccctctggga acgtttgtct aaaggaaatg
     4561 tttctgttca gtgtaacaat tacagttgca cctggattgc ccagtcctgc ccctgcacta
     4621 ggggaccatt aatcactgca aagtagaaga attattaagt taaaccagag tttgagccaa
     4681 gaaaacccct gaacaatgtt catcttctgt gaaacttgct caaatagtta agcttaacca
     4741 tgttgctgcc aaagactttt cctatgcagt ggtggggcac cttgatcatc atcattatct
     4801 tgattggctg aaaaaaaaat agttttaagc acacaccact gtctatgaga actgcaaatt
     4861 gggagaatag gtgaaatgca gaatctgaga gaacgcgaga agatgagatc attacagggt
     4921 ggaaagttct gcagcagcct tttctggtaa tccctttctg cagaacctga tgtttatggg
     4981 ctctaaaacg cagcttagct ttagaagcaa cagaaagcat gaaatagggt gtccatttta
     5041 aatgtgttcc tgcaactttt ttcattaaaa ctttgagggc ccaattttaa tttgtggaat
     5101 attcccgtta ataatgagat ctaattaaga catccattaa aagcccgtta aagttaattt
     5161 aacgtaaaaa ttccaataga actgtattag attttctcca ttaaattaac gttatggatt
     5221 tttaacggat gtcttaatta tacgttatta ttaacgggaa tactgtatta cacagattaa
     5281 aatcaggtcc taagtcaact tggaagagct aagagcatgt tttaatatta aaagtcttgc
     5341 atacctagtg cacagtttgg agacgcaagg atagatctgt ttactctagt tgaacatttt
     5401 ctatacaatt gaaagcaacc tataatagat aaatccatca ttgcatttaa acaatgaatt
     5461 tccttattct caaaggacaa atacgtctgg attatgtggt aaattgctac tcagctatgg
     5521 tgaaatattt atactattct aggcacaaca ctaggaacta ggtgattctg aaacaaaagg
     5581 aatattttct gttgttgctt taattaccaa ggttattttt ttttaatctc aacactgaca
     5641 aaatgaaacc aaatatctct tcctcaccat ttctcaagga ggctgcctgt tggaattgtt
     5701 ttggaaattt tgacatgatc cctaaattca acattgggat taaaaaaaaa aaaaaacttc
     5761 ttatttacct cctagggaaa gtgttgccct tatgccacat ataatagcaa attgcttttt
     5821 ttatggcatg cataacctag atgggaaaaa atatggcgct tcggggaagg agggaaaaag
     5881 taaatgaagt tccaggaatg tcattctgaa gtaatgaggc atggacagaa aatatacccc
     5941 tcacatcatc ggattgagat ggcagtcgaa atagcttcat tgaagtgtca gcactcatcc
     6001 atcaatcaat cacccacaag gaaaaatagc aacagtacaa cggggtggct tttatgggat
     6061 ttactcatgg gcatagggaa tagcggctca aatgtagttc tgacatgaaa agcaaggtgc
     6121 tgatattatt ttttatgatg ggaggatcat aaagtgaatt gagaacagtg aggtctgtct
     6181 ttgcttaacc tattcaacca gaaatgaatg gagctcgact ggaaaggaac agtcttcaga
     6241 tgggttaaga ttgaagggtg gactggactc tactgagcac cgtccttcaa caaggaaatt
     6301 ctattaaagg aaaatcaatg cattagtatt ggggttctcg tagctgttaa aaattgtctg
     6361 ctccaatcca gggttattag gccaaagtta cataattcag atctcactgc aaccatccaa
     6421 aagtggattc tcgagccctt gctccaatgg ggggaggaga tcaatacaat tcccaattcc
     6481 atggaaattg tttcccttct aaggaagaaa aaataaatca tctgcttcaa catataatcg
     6541 atatggtttt gttagcgtaa tttctatggt gggtggggtg ggaggtgaga gaaaaaaata
     6601 ttgataaatt tggtaagaca ggtgaattgc cgcctggcaa ccgtgcatgt cactgccgag
     6661 ggatggctgc taaggttcac cttagaaaac aagatctggg ctggcactgg ggcatacatc
     6721 accactcagc atattcctag aggccaggcc tgtcttcact cagccagccc tctgaggctt
     6781 ctagaaactt ctttctggag gaaaaaaact aaataacata aactcaggag aatgtcttta
     6841 cccaccttca taccactgct ttctttttgc tgaataaaac acagttctga taagtaagaa
     6901 ctttagaatt ggaaaggagg ctgacatgca aatataatgc aaattaccct caagtatcgc
     6961 cattcttcca ccacctcttg gtaccagtga gagcgagaga ttgccttttc ttccccatcc
     7021 ctccttccag ctaagaccac caaccagctg caaattgaga tgtccattta aaaatttata
     7081 tgtcaatatt taaatgttac atatttggcc ctattttgta gttcagcaaa tcctccaaat
     7141 acacagcatg ttacaaggca ctggtggcac agggcacaac aggaaatgat atttatttag
     7201 caaattcatt taacaaatat tattgggcac ctgttatgtg agacactgtc ctaggcactg
     7261 tgggataaca acagcaaaca cttcacacaa cagcctggcc ttcctgtgtt ttacaacagc
     7321 tcctaaagat agctgatatc aagacatttg agggacacag ttcatgtaga atcaaaatat
     7381 tagtatttca gaataaggat tttttttctg aaaagcatac agagaggaaa cagcttaaaa
     7441 ataggtcaag acctaaaaac agaatataat cacggaataa actggataac ccagacagtc
     7501 cccacagaat ttctttcagg tcacagattt cttaaaactc acccccaaaa tgtgcctgct
     7561 tggttgtttg aatcttgcat aattaatgtc acaggcgcaa gccgctgaac ttagttgaga
     7621 tgcagaaaac aaacaaatgc aatgacatat ctgagaagca tttatgtaac tccggttaag
     7681 tggtgaggag gggtgtgtga agacagtgtg catgcatgag tgtgtattca tatatatgtg
     7741 tatacatatg aatttcactg ttattttcca gggtctatgg acaatgtggc agtaagagtc
     7801 tatgatgttc tgaaactttt cacagtaaat ccaaagatta cagaccttac aaggtgcttg
     7861 cattctgttg cttttccatc tgtcacttct caggttattt gactgtgttc aaaccttctt
     7921 ttctttttca ttgagtttca ttttttaagc ttgttaaatg cttttgttta aaaaaaaaaa
     7981 aaaaacccca aatgtcattt ttcacattat cctctcttct ctgcaacaag gatagtaaga
     8041 tgtagatgaa tgcaaaaata ataacaacaa taaggaaata tattaaagct ttaaaatatg
     8101 cacatatgta gttctaaaga gcaataacgg tagtatctat ttcgaacatg cattaggcaa
     8161 aaaagaaatc aaaactgaaa ttttcgtgta tttttcccct tgtaagatgt tcaaatgcta
     8221 acttcatttt ctcctttcct ctatgtggca ctttctcaaa atatctatga aatactttta
     8281 gacaaagatt gagctggaga aagagataca aatttccatc cccccagaca gagagacata
     8341 tttccattgt aggaaggcat taaacatttt gaaacttgtg aatcatcttt agaatttcta
     8401 ctggggaatt ttacttcttc atccaaagta aaagccactt atctcctttg gttcccagtg
     8461 acagattcag aggcatacgc agatatacaa ttttcaggct ctagttaatc ttcttccaat
     8521 agttacgaac aatgggctaa caggcgtggg tgtttctcca aaaattattc atgcacaagg
     8581 cagcccaaag cttcagggaa aactagaaat gtgttatgga ttagaatagg actgttttaa
     8641 aatgctagta ccaggtggaa cgctatttct gcaacaggac tctgtccatt tcctttggaa
     8701 caatatattc caagtaaaat ggctcttcca aggaatgaca cctttacttg acacccttcg
     8761 gcatacaaat gattttacca atagccatga ttattattaa ggccttttaa aatacaggct
     8821 gtttgaaaaa agacagatta aatattcaca gcctttgtat catggttatt tgcttaaaac
     8881 agcttttaga agtacaagta ataacttttt gataagaaac cccaggagaa actttttggt
     8941 aagaaacctc aaaaaatttg aacaaaggca ttacaaaaaa aaaaaaaaaa ctaaccactc
     9001 cattcaactc tctcagaaaa taaatttcaa tgtgttcaat gaattgtctt gaacctgaaa
     9061 cctgcattta gatatcagtc ccctgccaat agctaatatt aacagaattt gaacaatcat
     9121 acaattatgt ctcaaatgtg aagactttgt acagtaatat tttcactttc taaatgaccc
     9181 atataacatt caggaattat agatgtgtat gtatattttt taagtacaga aagttcagcc
     9241 agtcttcaga gaagtaaaag tgatgtctat tgtgcattga agtaaatatt acaaacattc
     9301 cagtttcgca atacaatact tgagctttcg aacacctcag acactagaat gtgtaatgcg
     9361 agtcaaaaaa gctgacatac aaaacaattc ccatttggct cagggttcct aaatgtcaca
     9421 atatcttggg taaaatatac tttttgattt cctgatgatg tccttctaat cccttctgac
     9481 tttgattcct aacagccagg cactgttgac atgaatcatt aacttccaaa cccctttaaa
     9541 atcaagaagc taggtgatca tacagtcatt tcaatggcca accagttctt gctctacaga
     9601 gcttttacac ctttttggga aacctgatat caaacacatt tatgttatat atttgctccc
     9661 ttgcattaat tctagatttt tttttaattt cttttagaaa gggcaggggg gaagtgggtc
     9721 agagcaaggt tcaagaatca cattcatcct tgctctaaag tgtttacttg ccagcaaaga
     9781 aaggcaaaca catttttata ttcagaaagc agaccggtca ttttcaaaga aaaatgactg
     9841 caaccatgcc tgtagaatgt ttctgtgcaa gcgcactaat tttctatcac ctgcatgctg
     9901 tatataatac atttgcctgt atactaggaa gaaaaaccag gctgttttcc ctgagtacaa
     9961 tgcagcttgg atggctggga gcgtaagcct tccgtgcatt tttatagtgt acatatttgt
    10021 atatactaac tatatcgcca tgtatgaaca cagattttgt tatatttgct tgtttctgtt
    10081 tcctaccaaa ctggcccaca atggggattc ttttgtatag aaaaaatatg cttgtaattt
    10141 tttcctggtc attctctttc aatagcttat gaaagaatta gatctgagtt tacaaagaaa
    10201 ctataagaac caagtttgtc tgtctgcatg agtcccgtcc aattgctgga tctagggagg
    10261 aaccaacttc ctaattcaga gttttccttt taaaggcatg ctttaccccc atgggaaaac
    10321 tgcacactca tccatgtaga attattctct ttgtatttta tctaatagtg cctgaaaatt
    10381 tttttaatgt cttcttagaa gaagaattca taattgtcaa aatttgaaac attagcttaa
    10441 ttttgttttt atgacctcaa gattcttctc cttatttatt cggttgctgt tgtaatgggg
    10501 ccccaggcca ttcctgacat cggcgtgttc ttcttctgca ttaaggatgt ttttgaaatt
    10561 acagagatta ttgagccaac aggctgtttt aatcaaaacc atgtttcact tctttttgat
    10621 gattataaat tgtccttgca atgaaaaaaa aaaaagaact tttctgctag gaagattata
    10681 ccaccctgtg gccaaacaga ttcatcacag ataggcatct atgcccattt ctctgggatc
    10741 tggaaaattc ttcccttggc tgaccccaat ttcttttact ccccattatc ctgaatatta
    10801 gctttcaatg cagtcactat ttgacatttc caaaggcttt gccgcattgt cactgcccaa
    10861 agacaaacaa ccactggaaa tgatggcttt cctgcttgaa acgaaggggg ccaggtgcag
    10921 tggctcaagc ctgtaacccc tgcactttgg aaggctgagg caggcggatc acttgaggtc
    10981 aggagtttca gaccaacctg gccaacatgg caaaacctcg tctctactaa aaatacaaaa
    11041 aacattagca gggcatggtg gtgcgtgcct gtagtcccag ctacttggga ggctgaggca
    11101 agagaattgc ttgagcccgg gaggcgaagg ttgcagtgag ctgagatggt gccactgcac
    11161 tccagcctgg gcaacagagc aagactgtgt ctcaaaaaaa aaaaaaagaa tggattttca
    11221 gaaaaagtgc tccctttcct gtcctgtggt gccaccatcc tgtcctcctt cgtaatcatg
    11281 aacaatctga tcttgaactc ccacataact taaatcaggc aaaaagaaac attcacagcg
    11341 tccccttgct gaataaaaat gactttgttt ggaggcactt aagatgtatg cctgtgtgtg
    11401 gtgccgcagc attgaaatta tctgtagaag gggaattttt tttaaaaata caattttatc
    11461 actagaaata aattccgatg gtggaaacga agaaaaccct taaattatat cacaaaagcc
    11521 attatttttt gcatccaaag agtttttttt tttaaggaaa atcattctac tttgagaact
    11581 gtaattaaag ccctaaataa cagacactac tttgttgagc tattgtgaaa aaaaaacaac
    11641 acattcgcca aggttatatg gagcccctga tttccatcaa aaaggtttct ataagtatat
    11701 tatttacatt tttatacatg ataactcttg cctttgtgtt gaaaaaaaaa aagtctcttt
    11761 tttttccccc actcagcagt tattggaaat agactgttcc catctgaaac cgtatcgtaa
    11821 tttgcatcag gaaacccaac tgctgacatt gaggacctgg gtgtgttcaa ttatgatttt
    11881 gctggaggct gtccctcatt ttaatgctgc agctattgaa ccaccttcct gaaacctagc
    11941 tgatacggaa tagcagagac atgcctctca acaccattag ctttgcaaat ggcttcattt
    12001 cagtcaacgt cgacttctgc tttggccaat tgaaaaatga aaattaaagg agagaagaaa
    12061 aaaaacacag atgcacttaa aacatgaaaa gaattattta tatgataaaa atatatttag
    12121 cttttcaaag cacaagactg aatagaagtg ctctttttat gctttctgga gatgttactg
    12181 ttaaatgtct ttctacatca ggcttaataa atctgtaatg acatttgatg gattga
//
