LOCUS       NM_001277991             364 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens thyroid stimulating hormone subunit beta (TSHB),
            transcript variant 2, mRNA.
ACCESSION   NM_001277991
VERSION     NM_001277991.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Mezei,M., Baliram,R., Ali,M.R., Zaidi,M., Davies,T.F. and Latif,R.
  TITLE     The Human TSHbeta Subunit Proteins and Their Binding Sites on the
            TSH Receptor Using Molecular Dynamics Simulation
  JOURNAL   Endocrinology 161 (9) (2020)
   PUBMED   32738139
  REMARK    GeneRIF: The Human TSHbeta Subunit Proteins and Their Binding Sites
            on the TSH Receptor Using Molecular Dynamics Simulation.
REFERENCE   2  (bases 1 to 364)
  AUTHORS   Zhou,W., Brumpton,B., Kabil,O., Gudmundsson,J., Thorleifsson,G.,
            Weinstock,J., Zawistowski,M., Nielsen,J.B., Chaker,L., Medici,M.,
            Teumer,A., Naitza,S., Sanna,S., Schultheiss,U.T., Cappola,A.,
            Karjalainen,J., Kurki,M., Oneka,M., Taylor,P., Fritsche,L.G.,
            Graham,S.E., Wolford,B.N., Overton,W., Rasheed,H., Haug,E.B.,
            Gabrielsen,M.E., Skogholt,A.H., Surakka,I., Davey Smith,G.,
            Pandit,A., Roychowdhury,T., Hornsby,W.E., Jonasson,J.G., Senter,L.,
            Liyanarachchi,S., Ringel,M.D., Xu,L., Kiemeney,L.A., He,H.,
            Netea-Maier,R.T., Mayordomo,J.I., Plantinga,T.S., Hrafnkelsson,J.,
            Hjartarson,H., Sturgis,E.M., Palotie,A., Daly,M., Citterio,C.E.,
            Arvan,P., Brummett,C.M., Boehnke,M., de la Chapelle,A.,
            Stefansson,K., Hveem,K., Willer,C.J. and Asvold,B.O.
  TITLE     GWAS of thyroid stimulating hormone highlights pleiotropic effects
            and inverse association with thyroid cancer
  JOURNAL   Nat Commun 11 (1), 3981 (2020)
   PUBMED   32769997
  REMARK    GeneRIF: GWAS of thyroid stimulating hormone highlights pleiotropic
            effects and inverse association with thyroid cancer.
            Erratum:[Nat Commun. 2021 Dec 16;12(1):7354. doi:
            10.1038/s41467-021-27675-w.. PMID: 34916535]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 364)
  AUTHORS   Kovanicova,Z., Kurdiova,T., Balaz,M., Stefanicka,P., Varga,L.,
            Kulterer,O.C., Betz,M.J., Haug,A.R., Burger,I.A., Kiefer,F.W.,
            Wolfrum,C., Ukropcova,B. and Ukropec,J.
  TITLE     Cold Exposure Distinctively Modulates Parathyroid and Thyroid
            Hormones in Cold-Acclimatized and Non-Acclimatized Humans
  JOURNAL   Endocrinology 161 (7) (2020)
   PUBMED   32242612
  REMARK    GeneRIF: Cold Exposure Distinctively Modulates Parathyroid and
            Thyroid Hormones in Cold-Acclimatized and Non-Acclimatized Humans.
REFERENCE   4  (bases 1 to 364)
  AUTHORS   Liu,C., Li,L., Ying,F., Xu,C., Zang,X. and Gao,Z.
  TITLE     A newly identified TSHbeta splice variant is involved in the
            pathology of Hashimoto's thyroiditis
  JOURNAL   Mol Biol Rep 39 (12), 10019-10030 (2012)
   PUBMED   22752807
  REMARK    GeneRIF: In Hashimoto's thyroiditis the hTSHbeta splice variant
            exists in human serum and dimerises with TSHalpha.
REFERENCE   5  (bases 1 to 364)
  AUTHORS   Schaefer,J.S. and Klein,J.R.
  TITLE     A novel thyroid stimulating hormone beta-subunit isoform in human
            pituitary, peripheral blood leukocytes, and thyroid
  JOURNAL   Gen Comp Endocrinol 162 (3), 241-244 (2009)
   PUBMED   19364510
  REMARK    GeneRIF: Human pituitary, thyroid, and mononuclear leukocytes
            express a TSHbeta isoform that is analogous to the mouse TSHbeta
            isoform, consisting of a 27 nucleotide portion of intron 2 and all
            of exon 3, coding for 71.2% of the native human TSHbeta
            polypeptide.
            Erratum:[Gen Comp Endocrinol. 2009 Nov-Dec;164(2-3):130]
REFERENCE   6  (bases 1 to 364)
  AUTHORS   Steinfelder,H.J., Radovick,S., Mroczynski,M.A., Hauser,P.,
            McClaskey,J.H., Weintraub,B.D. and Wondisford,F.E.
  TITLE     Role of a pituitary-specific transcription factor (pit-1/GHF-1) or
            a closely related protein in cAMP regulation of human
            thyrotropin-beta subunit gene expression
  JOURNAL   J Clin Invest 89 (2), 409-419 (1992)
   PUBMED   1310694
REFERENCE   7  (bases 1 to 364)
  AUTHORS   Tokino,T., Hayashizaki,Y., Takata,K.I., Yoshida,M.C. and
            Matsubara,K.
  TITLE     Chromosome regional mapping for the human thyroid stimulating
            hormone beta subunit (TSHB) gene
  JOURNAL   Cytogenet Cell Genet 53 (2-3), 140-143 (1990)
   PUBMED   2369842
REFERENCE   8  (bases 1 to 364)
  AUTHORS   Tatsumi,K., Hayashizaki,Y., Hiraoka,Y., Miyai,K. and Matsubara,K.
  TITLE     The structure of the human thyrotropin beta-subunit gene
  JOURNAL   Gene 73 (2), 489-497 (1988)
   PUBMED   3243440
REFERENCE   9  (bases 1 to 364)
  AUTHORS   Sairam,M.R. and Li,C.H.
  TITLE     Human pituitary thyrotropin. The primary structure of the alpha and
            beta subunits
  JOURNAL   Can J Biochem 55 (7), 755-760 (1977)
   PUBMED   890569
REFERENCE   10 (bases 1 to 364)
  AUTHORS   Isomaa,B. and Sjoblom,G.
  TITLE     The effect of CTAB, a cationic surfactant, on the absorption rate
            of [14C]tripalmitate from a test meal in the rat
  JOURNAL   Food Cosmet Toxicol 13 (5), 517-520 (1975)
   PUBMED   290
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL109660.14.
            
            Summary: The four human glycoprotein hormones chorionic
            gonadotropin (CG), luteinizing hormone (LH), follicle stimulating
            hormone (FSH), and thyroid stimulating hormone (TSH) are dimers
            consisting of alpha and beta subunits that are associated
            noncovalently. The alpha subunits of these hormones are identical,
            however, their beta chains are unique and confer biological
            specificity. Thyroid stimulating hormone functions in the control
            of thyroid structure and metabolism. The protein encoded by this
            gene is the beta subunit of thyroid stimulating hormone. Mutations
            in this gene are associated with congenital central and secondary
            hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of
            this gene results in multiple transcript variants. [provided by
            RefSeq, May 2013].
            
            Transcript Variant: This variant (2) initiates translation at an
            alternate start codon and contains an intronless coding sequence,
            compared to variant 1. The encoded isoform (2, also known as novel
            TSH-beta) has a distinct N-terminus and is shorter than isoform 1.
            This variant is based on experimental data in PMIDs 19364510 and
            22752807.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on data in published reports (PMIDs 19364510 and
            22752807).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-364               AL109660.14        32171-32534
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p13.2"
     gene            1..364
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /note="thyroid stimulating hormone subunit beta"
                     /db_xref="GeneID:7252"
                     /db_xref="HGNC:HGNC:12372"
                     /db_xref="MIM:188540"
     exon            1..364
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /inference="alignment:Splign:2.1.0"
     CDS             1..282
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /note="isoform 2 is encoded by transcript variant 2;
                     thyrotropin subunit beta; thyrotropin beta chain; thyroid
                     stimulating hormone beta"
                     /codon_start=1
                     /product="thyrotropin subunit beta isoform 2"
                     /protein_id="NP_001264920.1"
                     /db_xref="GeneID:7252"
                     /db_xref="HGNC:HGNC:12372"
                     /db_xref="MIM:188540"
                     /translation="MLSFLFFPQDINGKLFLPKYALSQDVCTYRDFIYRTVEIPGCPL
                     HVAPYFSYPVALSCKCGKCNTDYSDCIHEAIKTNYCTKPQKSYLVGFSV"
     regulatory      324..329
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /note="hexamer: AATAAA"
     polyA_site      357
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /note="major polyA site"
ORIGIN      
        1 atgctctctt ttctgttctt tccccaggat atcaatggca aactgtttct tcccaaatat
       61 gctctgtccc aggatgtttg cacatataga gacttcatct acaggactgt agaaatacca
      121 ggatgcccac tccatgttgc tccctatttt tcctatcctg ttgctttaag ctgtaagtgt
      181 ggcaagtgca atactgacta tagtgactgc atacatgaag ccatcaagac aaactactgt
      241 accaaacctc agaagtctta tctggtagga ttttctgtct aatagtgata taatttgcaa
      301 tttggttaaa tgtgcttgcc tgaaataaag ctaataaaaa tattatgttt cacattatct
      361 tctg
//
