LOCUS       NM_000549                528 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens thyroid stimulating hormone subunit beta (TSHB),
            transcript variant 1, mRNA.
ACCESSION   NM_000549
VERSION     NM_000549.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Mezei,M., Baliram,R., Ali,M.R., Zaidi,M., Davies,T.F. and Latif,R.
  TITLE     The Human TSHbeta Subunit Proteins and Their Binding Sites on the
            TSH Receptor Using Molecular Dynamics Simulation
  JOURNAL   Endocrinology 161 (9) (2020)
   PUBMED   32738139
  REMARK    GeneRIF: The Human TSHbeta Subunit Proteins and Their Binding Sites
            on the TSH Receptor Using Molecular Dynamics Simulation.
REFERENCE   2  (bases 1 to 528)
  AUTHORS   Zhou,W., Brumpton,B., Kabil,O., Gudmundsson,J., Thorleifsson,G.,
            Weinstock,J., Zawistowski,M., Nielsen,J.B., Chaker,L., Medici,M.,
            Teumer,A., Naitza,S., Sanna,S., Schultheiss,U.T., Cappola,A.,
            Karjalainen,J., Kurki,M., Oneka,M., Taylor,P., Fritsche,L.G.,
            Graham,S.E., Wolford,B.N., Overton,W., Rasheed,H., Haug,E.B.,
            Gabrielsen,M.E., Skogholt,A.H., Surakka,I., Davey Smith,G.,
            Pandit,A., Roychowdhury,T., Hornsby,W.E., Jonasson,J.G., Senter,L.,
            Liyanarachchi,S., Ringel,M.D., Xu,L., Kiemeney,L.A., He,H.,
            Netea-Maier,R.T., Mayordomo,J.I., Plantinga,T.S., Hrafnkelsson,J.,
            Hjartarson,H., Sturgis,E.M., Palotie,A., Daly,M., Citterio,C.E.,
            Arvan,P., Brummett,C.M., Boehnke,M., de la Chapelle,A.,
            Stefansson,K., Hveem,K., Willer,C.J. and Asvold,B.O.
  TITLE     GWAS of thyroid stimulating hormone highlights pleiotropic effects
            and inverse association with thyroid cancer
  JOURNAL   Nat Commun 11 (1), 3981 (2020)
   PUBMED   32769997
  REMARK    GeneRIF: GWAS of thyroid stimulating hormone highlights pleiotropic
            effects and inverse association with thyroid cancer.
            Erratum:[Nat Commun. 2021 Dec 16;12(1):7354. doi:
            10.1038/s41467-021-27675-w.. PMID: 34916535]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 528)
  AUTHORS   Kovanicova,Z., Kurdiova,T., Balaz,M., Stefanicka,P., Varga,L.,
            Kulterer,O.C., Betz,M.J., Haug,A.R., Burger,I.A., Kiefer,F.W.,
            Wolfrum,C., Ukropcova,B. and Ukropec,J.
  TITLE     Cold Exposure Distinctively Modulates Parathyroid and Thyroid
            Hormones in Cold-Acclimatized and Non-Acclimatized Humans
  JOURNAL   Endocrinology 161 (7) (2020)
   PUBMED   32242612
  REMARK    GeneRIF: Cold Exposure Distinctively Modulates Parathyroid and
            Thyroid Hormones in Cold-Acclimatized and Non-Acclimatized Humans.
REFERENCE   4  (bases 1 to 528)
  AUTHORS   Liu,C., Li,L., Ying,F., Xu,C., Zang,X. and Gao,Z.
  TITLE     A newly identified TSHbeta splice variant is involved in the
            pathology of Hashimoto's thyroiditis
  JOURNAL   Mol Biol Rep 39 (12), 10019-10030 (2012)
   PUBMED   22752807
  REMARK    GeneRIF: In Hashimoto's thyroiditis the hTSHbeta splice variant
            exists in human serum and dimerises with TSHalpha.
REFERENCE   5  (bases 1 to 528)
  AUTHORS   Schaefer,J.S. and Klein,J.R.
  TITLE     A novel thyroid stimulating hormone beta-subunit isoform in human
            pituitary, peripheral blood leukocytes, and thyroid
  JOURNAL   Gen Comp Endocrinol 162 (3), 241-244 (2009)
   PUBMED   19364510
  REMARK    GeneRIF: Human pituitary, thyroid, and mononuclear leukocytes
            express a TSHbeta isoform that is analogous to the mouse TSHbeta
            isoform, consisting of a 27 nucleotide portion of intron 2 and all
            of exon 3, coding for 71.2% of the native human TSHbeta
            polypeptide.
            Erratum:[Gen Comp Endocrinol. 2009 Nov-Dec;164(2-3):130]
REFERENCE   6  (bases 1 to 528)
  AUTHORS   Steinfelder,H.J., Radovick,S., Mroczynski,M.A., Hauser,P.,
            McClaskey,J.H., Weintraub,B.D. and Wondisford,F.E.
  TITLE     Role of a pituitary-specific transcription factor (pit-1/GHF-1) or
            a closely related protein in cAMP regulation of human
            thyrotropin-beta subunit gene expression
  JOURNAL   J Clin Invest 89 (2), 409-419 (1992)
   PUBMED   1310694
REFERENCE   7  (bases 1 to 528)
  AUTHORS   Tokino,T., Hayashizaki,Y., Takata,K.I., Yoshida,M.C. and
            Matsubara,K.
  TITLE     Chromosome regional mapping for the human thyroid stimulating
            hormone beta subunit (TSHB) gene
  JOURNAL   Cytogenet Cell Genet 53 (2-3), 140-143 (1990)
   PUBMED   2369842
REFERENCE   8  (bases 1 to 528)
  AUTHORS   Tatsumi,K., Hayashizaki,Y., Hiraoka,Y., Miyai,K. and Matsubara,K.
  TITLE     The structure of the human thyrotropin beta-subunit gene
  JOURNAL   Gene 73 (2), 489-497 (1988)
   PUBMED   3243440
REFERENCE   9  (bases 1 to 528)
  AUTHORS   Sairam,M.R. and Li,C.H.
  TITLE     Human pituitary thyrotropin. The primary structure of the alpha and
            beta subunits
  JOURNAL   Can J Biochem 55 (7), 755-760 (1977)
   PUBMED   890569
REFERENCE   10 (bases 1 to 528)
  AUTHORS   Isomaa,B. and Sjoblom,G.
  TITLE     The effect of CTAB, a cationic surfactant, on the absorption rate
            of [14C]tripalmitate from a test meal in the rat
  JOURNAL   Food Cosmet Toxicol 13 (5), 517-520 (1975)
   PUBMED   290
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL109660.14.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 26, 2019 this sequence version replaced NM_000549.4.
            
            Summary: The four human glycoprotein hormones chorionic
            gonadotropin (CG), luteinizing hormone (LH), follicle stimulating
            hormone (FSH), and thyroid stimulating hormone (TSH) are dimers
            consisting of alpha and beta subunits that are associated
            noncovalently. The alpha subunits of these hormones are identical,
            however, their beta chains are unique and confer biological
            specificity. Thyroid stimulating hormone functions in the control
            of thyroid structure and metabolism. The protein encoded by this
            gene is the beta subunit of thyroid stimulating hormone. Mutations
            in this gene are associated with congenital central and secondary
            hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of
            this gene results in multiple transcript variants. [provided by
            RefSeq, May 2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1, also known as native
            TSH-beta).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments and data in published reports (PMID
            3243440).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: BG202613.1, BC069298.1 [ECO:0000331]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000256592.3/ ENSP00000256592.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AL109660.14        28051-28085
            36-198              AL109660.14        31587-31749
            199-528             AL109660.14        32198-32527
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p13.2"
     gene            1..528
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /note="thyroid stimulating hormone subunit beta"
                     /db_xref="GeneID:7252"
                     /db_xref="HGNC:HGNC:12372"
                     /db_xref="MIM:188540"
     exon            1..35
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            36..198
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..453
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; thyrotropin subunit beta; thyrotropin beta
                     chain; thyroid stimulating hormone beta"
                     /codon_start=1
                     /product="thyrotropin subunit beta isoform 1 precursor"
                     /protein_id="NP_000540.2"
                     /db_xref="CCDS:CCDS880.1"
                     /db_xref="GeneID:7252"
                     /db_xref="HGNC:HGNC:12372"
                     /db_xref="MIM:188540"
                     /translation="MTALFLMSMLFGLTCGQAMSFCIPTEYTMHIERRECAYCLTINT
                     TICAGYCMTRDINGKLFLPKYALSQDVCTYRDFIYRTVEIPGCPLHVAPYFSYPVALS
                     CKCGKCNTDYSDCIHEAIKTNYCTKPQKSYLVGFSV"
     exon            199..528
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      495..500
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /note="hexamer: AATAAA"
     polyA_site      528
                     /gene="TSHB"
                     /gene_synonym="TSH-B; TSH-BETA"
                     /note="major polyA site"
ORIGIN      
        1 gtcaccacag catctgctca ccaatgcaaa gtaagcatga ctgctctctt tctgatgtcc
       61 atgctttttg gccttacatg tgggcaagcg atgtcttttt gtattccaac tgagtataca
      121 atgcacatcg aaaggagaga gtgtgcttat tgcctaacca tcaacaccac catctgtgct
      181 ggatattgta tgacacggga tatcaatggc aaactgtttc ttcccaaata tgctctgtcc
      241 caggatgttt gcacatatag agacttcatc tacaggactg tagaaatacc aggatgccca
      301 ctccatgttg ctccctattt ttcctatcct gttgctttaa gctgtaagtg tggcaagtgc
      361 aatactgact atagtgactg catacatgaa gccatcaaga caaactactg taccaaacct
      421 cagaagtctt atctggtagg attttctgtc taatagtgat ataatttgca atttggttaa
      481 atgtgcttgc ctgaaataaa gctaataaaa atattatgtt tcacatta
//
