LOCUS       NM_001303264           11110 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens TSC22 domain family member 2 (TSC22D2), transcript
            variant 2, mRNA.
ACCESSION   NM_001303264 XM_005247920
VERSION     NM_001303264.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Xiao,L., Wei,F., Liang,F., Li,Q., Deng,H., Tan,S., Chen,S.,
            Xiong,F., Guo,C., Liao,Q., Li,X., Zhang,W., Wu,M., Zhou,Y.,
            Xiang,B., Zhou,M., Li,X., Xiong,W., Zeng,Z. and Li,G.
  TITLE     TSC22D2 identified as a candidate susceptibility gene of
            multi-cancer pedigree using genome-wide linkage analysis and
            whole-exome sequencing
  JOURNAL   Carcinogenesis 40 (7), 819-827 (2019)
   PUBMED   31125406
  REMARK    GeneRIF: Genome-wide linkage analysis and whole-exome sequencing in
            a rare, large multi-cancer pedigree recently found in China
            identified a novel mutant of transforming growth factor beta
            stimulated clone 22 domain family, member 2 (TSC22D2, c.-91T-C)
            cosegregated with the cancer phenotype. Study found that histone H4
            transcription factor (HINFP) binds to the promoter region of
            TSC22D2 and may regulate its transcription.
REFERENCE   2  (bases 1 to 11110)
  AUTHORS   Liang,F., Li,Q., Li,X., Li,Z., Gong,Z., Deng,H., Xiang,B., Zhou,M.,
            Li,X., Li,G., Zeng,Z. and Xiong,W.
  TITLE     TSC22D2 interacts with PKM2 and inhibits cell growth in colorectal
            cancer
  JOURNAL   Int J Oncol 49 (3), 1046-1056 (2016)
   PUBMED   27573352
  REMARK    GeneRIF: Results found TSC22D2 significantly downregulated in
            colorectal cancer (CRC). Its overexpression can inhibit CRC cell
            growth. Also, TSC22D2 physically associates with PKM2, and
            demonstrated that TSC22D2 overexpression reduces nuclear PKM2
            levels and represses the expression of cyclin D1.
REFERENCE   3  (bases 1 to 11110)
  AUTHORS   Li,Q., Chen,P., Zeng,Z., Liang,F., Song,Y., Xiong,F., Li,X.,
            Gong,Z., Zhou,M., Xiang,B., Peng,C., Li,X., Chen,X., Li,G. and
            Xiong,W.
  TITLE     Yeast two-hybrid screening identified WDR77 as a novel interacting
            partner of TSC22D2
  JOURNAL   Tumour Biol 37 (9), 12503-12512 (2016)
   PUBMED   27337956
  REMARK    GeneRIF: TSC22D2 protein might be a member of the PRMT5 complex via
            direct binding of WDR77.
REFERENCE   4  (bases 1 to 11110)
  AUTHORS   Wilson,C.H., Crombie,C., van der Weyden,L., Poulogiannis,G.,
            Rust,A.G., Pardo,M., Gracia,T., Yu,L., Choudhary,J., Poulin,G.B.,
            McIntyre,R.E., Winton,D.J., March,H.N., Arends,M.J., Fraser,A.G.
            and Adams,D.J.
  TITLE     Nuclear receptor binding protein 1 regulates intestinal progenitor
            cell homeostasis and tumour formation
  JOURNAL   EMBO J 31 (11), 2486-2497 (2012)
   PUBMED   22510880
REFERENCE   5  (bases 1 to 11110)
  AUTHORS   Dastani,Z., Hivert,M.F., Timpson,N., Perry,J.R., Yuan,X.,
            Scott,R.A., Henneman,P., Heid,I.M., Kizer,J.R., Lyytikainen,L.P.,
            Fuchsberger,C., Tanaka,T., Morris,A.P., Small,K., Isaacs,A.,
            Beekman,M., Coassin,S., Lohman,K., Qi,L., Kanoni,S., Pankow,J.S.,
            Uh,H.W., Wu,Y., Bidulescu,A., Rasmussen-Torvik,L.J.,
            Greenwood,C.M., Ladouceur,M., Grimsby,J., Manning,A.K., Liu,C.T.,
            Kooner,J., Mooser,V.E., Vollenweider,P., Kapur,K.A., Chambers,J.,
            Wareham,N.J., Langenberg,C., Frants,R., Willems-Vandijk,K.,
            Oostra,B.A., Willems,S.M., Lamina,C., Winkler,T.W., Psaty,B.M.,
            Tracy,R.P., Brody,J., Chen,I., Viikari,J., Kahonen,M.,
            Pramstaller,P.P., Evans,D.M., St Pourcain,B., Sattar,N., Wood,A.R.,
            Bandinelli,S., Carlson,O.D., Egan,J.M., Bohringer,S., van
            Heemst,D., Kedenko,L., Kristiansson,K., Nuotio,M.L., Loo,B.M.,
            Harris,T., Garcia,M., Kanaya,A., Haun,M., Klopp,N., Wichmann,H.E.,
            Deloukas,P., Katsareli,E., Couper,D.J., Duncan,B.B.,
            Kloppenburg,M., Adair,L.S., Borja,J.B., Wilson,J.G., Musani,S.,
            Guo,X., Johnson,T., Semple,R., Teslovich,T.M., Allison,M.A.,
            Redline,S., Buxbaum,S.G., Mohlke,K.L., Meulenbelt,I.,
            Ballantyne,C.M., Dedoussis,G.V., Hu,F.B., Liu,Y., Paulweber,B.,
            Spector,T.D., Slagboom,P.E., Ferrucci,L., Jula,A., Perola,M.,
            Raitakari,O., Florez,J.C., Salomaa,V., Eriksson,J.G.,
            Frayling,T.M., Hicks,A.A., Lehtimaki,T., Smith,G.D.,
            Siscovick,D.S., Kronenberg,F., van Duijn,C., Loos,R.J.,
            Waterworth,D.M., Meigs,J.B., Dupuis,J., Richards,J.B., Voight,B.F.,
            Scott,L.J., Steinthorsdottir,V., Dina,C., Welch,R.P., Zeggini,E.,
            Huth,C., Aulchenko,Y.S., Thorleifsson,G., McCulloch,L.J.,
            Ferreira,T., Grallert,H., Amin,N., Wu,G., Willer,C.J.,
            Raychaudhuri,S., McCarroll,S.A., Hofmann,O.M., Segre,A.V., van
            Hoek,M., Navarro,P., Ardlie,K., Balkau,B., Benediktsson,R.,
            Bennett,A.J., Blagieva,R., Boerwinkle,E., Bonnycastle,L.L.,
            Bostrom,K.B., Bravenboer,B., Bumpstead,S., Burtt,N.P.,
            Charpentier,G., Chines,P.S., Cornelis,M., Crawford,G., Doney,A.S.,
            Elliott,K.S., Elliott,A.L., Erdos,M.R., Fox,C.S., Franklin,C.S.,
            Ganser,M., Gieger,C., Grarup,N., Green,T., Griffin,S., Groves,C.J.,
            Guiducci,C., Hadjadj,S., Hassanali,N., Herder,C., Isomaa,B.,
            Jackson,A.U., Johnson,P.R., Jorgensen,T., Kao,W.H., Kong,A.,
            Kraft,P., Kuusisto,J., Lauritzen,T., Li,M., Lieverse,A.,
            Lindgren,C.M., Lyssenko,V., Marre,M., Meitinger,T., Midthjell,K.,
            Morken,M.A., Narisu,N., Nilsson,P., Owen,K.R., Payne,F.,
            Petersen,A.K., Platou,C., Proenca,C., Prokopenko,I., Rathmann,W.,
            Rayner,N.W., Robertson,N.R., Rocheleau,G., Roden,M., Sampson,M.J.,
            Saxena,R., Shields,B.M., Shrader,P., Sigurdsson,G., Sparso,T.,
            Strassburger,K., Stringham,H.M., Sun,Q., Swift,A.J., Thorand,B.,
            Tichet,J., Tuomi,T., van Dam,R.M., van Haeften,T.W., van Herpt,T.,
            van Vliet-Ostaptchouk,J.V., Walters,G.B., Weedon,M.N., Wijmenga,C.,
            Witteman,J., Bergman,R.N., Cauchi,S., Collins,F.S., Gloyn,A.L.,
            Gyllensten,U., Hansen,T., Hide,W.A., Hitman,G.A., Hofman,A.,
            Hunter,D.J., Hveem,K., Laakso,M., Morris,A.D., Palmer,C.N.,
            Rudan,I., Sijbrands,E., Stein,L.D., Tuomilehto,J., Uitterlinden,A.,
            Walker,M., Watanabe,R.M., Abecasis,G.R., Boehm,B.O., Campbell,H.,
            Daly,M.J., Hattersley,A.T., Pedersen,O., Barroso,I., Groop,L.,
            Sladek,R., Thorsteinsdottir,U., Wilson,J.F., Illig,T., Froguel,P.,
            van Duijn,C.M., Stefansson,K., Altshuler,D., Boehnke,M.,
            McCarthy,M.I., Soranzo,N., Wheeler,E., Glazer,N.L.,
            Bouatia-Naji,N., Magi,R., Randall,J., Elliott,P., Rybin,D.,
            Dehghan,A., Hottenga,J.J., Song,K., Goel,A., Lajunen,T., Doney,A.,
            Cavalcanti-Proenca,C., Kumari,M., Timpson,N.J., Zabena,C.,
            Ingelsson,E., An,P., O'Connell,J., Luan,J., Elliott,A.,
            McCarroll,S.A., Roccasecca,R.M., Pattou,F., Sethupathy,P.,
            Ariyurek,Y., Barter,P., Beilby,J.P., Ben-Shlomo,Y., Bergmann,S.,
            Bochud,M., Bonnefond,A., Borch-Johnsen,K., Bottcher,Y., Brunner,E.,
            Bumpstead,S.J., Chen,Y.D., Chines,P., Clarke,R., Coin,L.J.,
            Cooper,M.N., Crisponi,L., Day,I.N., de Geus,E.J., Delplanque,J.,
            Fedson,A.C., Fischer-Rosinsky,A., Forouhi,N.G., Franzosi,M.G.,
            Galan,P., Goodarzi,M.O., Graessler,J., Grundy,S., Gwilliam,R.,
            Hallmans,G., Hammond,N., Han,X., Hartikainen,A.L., Hayward,C.,
            Heath,S.C., Hercberg,S., Hillman,D.R., Hingorani,A.D., Hui,J.,
            Hung,J., Kaakinen,M., Kaprio,J., Kesaniemi,Y.A., Kivimaki,M.,
            Knight,B., Koskinen,S., Kovacs,P., Kyvik,K.O., Lathrop,G.M.,
            Lawlor,D.A., Le Bacquer,O., Lecoeur,C., Li,Y., Mahley,R.,
            Mangino,M., Martinez-Larrad,M.T., McAteer,J.B., McPherson,R.,
            Meisinger,C., Melzer,D., Meyre,D., Mitchell,B.D., Mukherjee,S.,
            Naitza,S., Neville,M.J., Orru,M., Pakyz,R., Paolisso,G.,
            Pattaro,C., Pearson,D., Peden,J.F., Pedersen,N.L., Pfeiffer,A.F.,
            Pichler,I., Polasek,O., Posthuma,D., Potter,S.C., Pouta,A.,
            Province,M.A., Rayner,N.W., Rice,K., Ripatti,S., Rivadeneira,F.,
            Rolandsson,O., Sandbaek,A., Sandhu,M., Sanna,S., Sayer,A.A.,
            Scheet,P., Seedorf,U., Sharp,S.J., Shields,B., Sigurethsson,G.,
            Sijbrands,E.J., Silveira,A., Simpson,L., Singleton,A., Smith,N.L.,
            Sovio,U., Swift,A., Syddall,H., Syvanen,A.C., Tonjes,A.,
            Uitterlinden,A.G., van Dijk,K.W., Varma,D., Visvikis-Siest,S.,
            Vitart,V., Vogelzangs,N., Waeber,G., Wagner,P.J., Walley,A.,
            Ward,K.L., Watkins,H., Wild,S.H., Willemsen,G., Witteman,J.C.,
            Yarnell,J.W., Zelenika,D., Zethelius,B., Zhai,G., Zhao,J.H.,
            Zillikens,M.C., Borecki,I.B., Meneton,P., Magnusson,P.K.,
            Nathan,D.M., Williams,G.H., Silander,K., Bornstein,S.R.,
            Schwarz,P., Spranger,J., Karpe,F., Shuldiner,A.R., Cooper,C.,
            Serrano-Rios,M., Lind,L., Palmer,L.J., Hu,F.B. 1st, Franks,P.W.,
            Ebrahim,S., Marmot,M., Kao,W.H., Pramstaller,P.P., Wright,A.F.,
            Stumvoll,M., Hamsten,A., Buchanan,T.A., Valle,T.T., Rotter,J.I.,
            Penninx,B.W., Boomsma,D.I., Cao,A., Scuteri,A., Schlessinger,D.,
            Uda,M., Ruokonen,A., Jarvelin,M.R., Peltonen,L., Mooser,V.,
            Sladek,R., Musunuru,K., Smith,A.V., Edmondson,A.C., Stylianou,I.M.,
            Koseki,M., Pirruccello,J.P., Chasman,D.I., Johansen,C.T.,
            Fouchier,S.W., Peloso,G.M., Barbalic,M., Ricketts,S.L., Bis,J.C.,
            Feitosa,M.F., Orho-Melander,M., Melander,O., Li,X., Li,M.,
            Cho,Y.S., Go,M.J., Kim,Y.J., Lee,J.Y., Park,T., Kim,K., Sim,X.,
            Ong,R.T., Croteau-Chonka,D.C., Lange,L.A., Smith,J.D., Ziegler,A.,
            Zhang,W., Zee,R.Y., Whitfield,J.B., Thompson,J.R., Surakka,I.,
            Spector,T.D., Smit,J.H., Sinisalo,J., Scott,J., Saharinen,J.,
            Sabatti,C., Rose,L.M., Roberts,R., Rieder,M., Parker,A.N., Pare,G.,
            O'Donnell,C.J., Nieminen,M.S., Nickerson,D.A., Montgomery,G.W.,
            McArdle,W., Masson,D., Martin,N.G., Marroni,F., Lucas,G., Luben,R.,
            Lokki,M.L., Lettre,G., Launer,L.J., Lakatta,E.G., Laaksonen,R.,
            Kyvik,K.O., Konig,I.R., Khaw,K.T., Kaplan,L.M., Johansson,A.,
            Janssens,A.C., Igl,W., Hovingh,G.K., Hengstenberg,C.,
            Havulinna,A.S., Hastie,N.D., Harris,T.B., Haritunians,T.,
            Hall,A.S., Groop,L.C., Gonzalez,E., Freimer,N.B., Erdmann,J.,
            Ejebe,K.G., Doring,A., Dominiczak,A.F., Demissie,S., Deloukas,P.,
            de Faire,U., Crawford,G., Chen,Y.D., Caulfield,M.J.,
            Boekholdt,S.M., Assimes,T.L., Quertermous,T., Seielstad,M.,
            Wong,T.Y., Tai,E.S., Feranil,A.B., Kuzawa,C.W., Taylor,H.A. Jr.,
            Gabriel,S.B., Holm,H., Gudnason,V., Krauss,R.M., Ordovas,J.M.,
            Munroe,P.B., Kooner,J.S., Tall,A.R., Hegele,R.A., Kastelein,J.J.,
            Schadt,E.E., Strachan,D.P., Reilly,M.P., Samani,N.J., Schunkert,H.,
            Cupples,L.A., Sandhu,M.S., Ridker,P.M., Rader,D.J. and
            Kathiresan,S.
  CONSRTM   DIAGRAM+ Consortium; MAGIC Consortium; GLGC Investigators; MuTHER
            Consortium; DIAGRAM Consortium; GIANT Consortium; Global B Pgen
            Consortium; Procardis Consortium; MAGIC investigators; GLGC
            Consortium
  TITLE     Novel loci for adiponectin levels and their influence on type 2
            diabetes and metabolic traits: a multi-ethnic meta-analysis of
            45,891 individuals
  JOURNAL   PLoS Genet 8 (3), e1002607 (2012)
   PUBMED   22479202
REFERENCE   6  (bases 1 to 11110)
  AUTHORS   Gluderer,S., Brunner,E., Germann,M., Jovaisaite,V., Li,C.,
            Rentsch,C.A., Hafen,E. and Stocker,H.
  TITLE     Madm (Mlf1 adapter molecule) cooperates with Bunched A to promote
            growth in Drosophila
  JOURNAL   J Biol 9 (1), 9 (2010)
   PUBMED   20149264
  REMARK    GeneRIF: Long form of TSC22DF is evolutionarily conserved and has
            growth-regulating potential.
REFERENCE   7  (bases 1 to 11110)
  AUTHORS   Vaquerizas,J.M., Kummerfeld,S.K., Teichmann,S.A. and Luscombe,N.M.
  TITLE     A census of human transcription factors: function, expression and
            evolution
  JOURNAL   Nat Rev Genet 10 (4), 252-263 (2009)
   PUBMED   19274049
  REMARK    Review article
REFERENCE   8  (bases 1 to 11110)
  AUTHORS   Gevaert,K., Goethals,M., Martens,L., Van Damme,J., Staes,A.,
            Thomas,G.R. and Vandekerckhove,J.
  TITLE     Exploring proteomes and analyzing protein processing by mass
            spectrometric identification of sorted N-terminal peptides
  JOURNAL   Nat Biotechnol 21 (5), 566-569 (2003)
   PUBMED   12665801
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC018545.14, AB014569.1, AK297748.1, BC044643.1, AA040291.1 and
            BU736250.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001303264.1.
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            coding region compared to variant 1. The encoded isoform (2) is
            shorter compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.12431.1,
                                           SRR7346977.1766880.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000688009.1/ ENSP00000510392.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AC018545.14        100308-100344
            38-2910             AB014569.1         1-2873
            2911-4147           AK297748.1         203-1439
            4148-4532           BC044643.1         3517-3901
            4533-4684           AA040291.1         133-284             c
            4685-4807           BU736250.1         16-138              c
            4808-11110          AC018545.14        152130-158432
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q25.1"
     gene            1..11110
                     /gene="TSC22D2"
                     /gene_synonym="TILZ4a; TILZ4b; TILZ4c"
                     /note="TSC22 domain family member 2"
                     /db_xref="GeneID:9819"
                     /db_xref="HGNC:HGNC:29095"
                     /db_xref="MIM:617724"
     exon            1..3011
                     /gene="TSC22D2"
                     /gene_synonym="TILZ4a; TILZ4b; TILZ4c"
                     /inference="alignment:Splign:2.1.0"
     CDS             1054..3324
                     /gene="TSC22D2"
                     /gene_synonym="TILZ4a; TILZ4b; TILZ4c"
                     /note="isoform 2 is encoded by transcript variant 2; TSC22
                     domain family 2; TSC22 domain family protein 2;
                     TSC22-related-inducible leucine zipper protein 4"
                     /codon_start=1
                     /product="TSC22 domain family protein 2 isoform 2"
                     /protein_id="NP_001290193.1"
                     /db_xref="CCDS:CCDS93406.1"
                     /db_xref="GeneID:9819"
                     /db_xref="HGNC:HGNC:29095"
                     /db_xref="MIM:617724"
                     /translation="MSKMPAKKKSCFQITSVTTAQVATSITEDTESLDDPDESRTEDV
                     SSEIFDVSRATDYGPEEVCERSSSEETLNNVGDAETPGTVSPNLLLDGQLAAAAAAPA
                     NGGGVVSARSVSGALASTLAAAATSAPAPGAPGGPQLAGSSAGPVTAAPSQPPTTCSS
                     RFRVIKLDHGSGEPYRRGRWTCMEYYERDSDSSVLTRSGDCIRHSSTFDQTAERDSGL
                     GATGGSVVVVVASMQGAHGPESGTDSSLTAVSQLPPSEKMSQPTPAQPQSFSVGQPQP
                     PPPPVGGAVAQSSAPLPPFPGAATGPQPMMAAAQPSQPQGAGPGGQTLPPTNVTLAQP
                     AMSLPPQPGPAVGAPAAQQPQQFAYPQPQIPPGHLLPVQPSGQSEYLQQHVAGLQPPS
                     PAQPSSTGAAASPATAATLPVGTGQNASSVGAQLMGASSQPSEAMAPRTGPAQGGQVA
                     PCQPTGVPPATVGGVVQPCLGPAGAGQPQSVPPPQMGGSGPLSAVPGGPHAVVPGVPN
                     VPAAVPAPSVPSVSTTSVTMPNVPAPLAQSQQLSSHTPVSRSSSIIQHVGLPLAPGTH
                     SAPTSLPQSDLSQFQTQTQPLVGQVDDTRRKSEPLPQPPLSLIAENKPVVKPPVADSL
                     ANPLQLTPMNSLATSVFSIAIPVDGDEDSASGGGVVAIDNKIEQAMDLVKSHLMYAVR
                     EEVEVLKEQIKELVERNSLLERENALLKSLSSNDQLSQLPTQQANPGSTSQQQAVIAQ
                     PPQPTQPPQQPNVSSA"
     exon            3012..3063
                     /gene="TSC22D2"
                     /gene_synonym="TILZ4a; TILZ4b; TILZ4c"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..11110
                     /gene="TSC22D2"
                     /gene_synonym="TILZ4a; TILZ4b; TILZ4c"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4621..4626
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC22D2"
                     /gene_synonym="TILZ4a; TILZ4b; TILZ4c"
                     /note="hexamer: AATAAA"
     polyA_site      4643
                     /gene="TSC22D2"
                     /gene_synonym="TILZ4a; TILZ4b; TILZ4c"
     regulatory      4785..4790
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC22D2"
                     /gene_synonym="TILZ4a; TILZ4b; TILZ4c"
                     /note="hexamer: ATTAAA"
     polyA_site      4807
                     /gene="TSC22D2"
                     /gene_synonym="TILZ4a; TILZ4b; TILZ4c"
                     /note="major polyA site"
     regulatory      11088..11093
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TSC22D2"
                     /gene_synonym="TILZ4a; TILZ4b; TILZ4c"
                     /note="hexamer: AATAAA"
     polyA_site      11110
                     /gene="TSC22D2"
                     /gene_synonym="TILZ4a; TILZ4b; TILZ4c"
ORIGIN      
        1 agtcggcagc ggcggcggca gcggggctgc tgctcctccc agcatccctg gcgcggccat
       61 ttcagcccca tcttggccca gcggagggag ctgcagccgc cgcttcagca gtttgaattt
      121 cagtttcttc ggggtttagg aattgggcgc accgaggagg agccggagaa gccaccaccg
      181 ctacctcaca cagccggggc gcctccggtc ccgtgccagc ggtctgccgc cgccgcccct
      241 ctctgagaga agccgcgagg ggaggccgag accgccgtcg ccgcccgccc gcaggggtgg
      301 ttccccctgt aaggggagga ccgagccggc tttcccctcc cccagagcgg gtttccgcct
      361 gaggcagtcg acatgtccct ggggctgagc tccggctaga gccggggagg gagggccgcc
      421 tgcccgcgcc acagccccac cgcccgcccg cggaccttca gccagctccg gcccgcggct
      481 cccggctgaa ttaggcatct ccgactccgg actcgccgct ccttccctca gtcagagagc
      541 ccagcgctga cgccggcacc ggcctgagga gcccgagcgg ggcagcaccg cccctcacgc
      601 cgccgccacc gcctcctcct cctcctcttc gccctcccac tcccaccccc agccaaggcc
      661 tgctgaccgc gccgagcgcc gagctggact gaccacggct gcccggagac gagagaggaa
      721 gcagccggcc cgcccctggg ttcgcgctct cgccgcctct gagggaattg aattgaggcg
      781 ccgcggctgc gagagctaaa aaggaaggag gagccgccgc gggactgaga cgggggcaga
      841 gccgaagaga ccgacacaga gaaggaaacg aggaggagga tgtctcaccg ggcggccagc
      901 gcctggatca gcccgtgact cttaacagcg gcgggcctca gaccccagcg cagactcgga
      961 ctttgtcttt gggggcccgt gctctgccct ccccggtttc cgacaggacc cagaggagcc
     1021 ggcgtgcctc tctgccctcc agccttcttc accatgtcca agatgccggc caagaagaag
     1081 agctgcttcc agatcaccag tgtcaccacg gcccaggtgg ccactagcat caccgaggac
     1141 accgagagct tggacgaccc ggacgagtca cgcacagagg acgtctcctc cgagattttc
     1201 gacgtctctc gggccacgga ttatggccct gaggaggtct gcgagcgcag ctcttccgaa
     1261 gagacgctta acaatgttgg ggatgcggag actcccggga ccgtctcccc aaacctcctc
     1321 ctagatgggc agctggcagc ggcggctgct gctcccgcca acggaggagg agtcgtttcg
     1381 gcccggagcg tgtctggggc gctcgccagt accctggcgg cggctgccac ttcggccccc
     1441 gcccccggag cacccggcgg cccccagctc gcgggctcat ccgccgggcc agtgactgca
     1501 gccccatctc agcctcccac cacatgtagt tcccgttttc gcgtgatcaa gctggaccac
     1561 gggagcggag agccctatag acgcggccga tggacgtgta tggaatacta tgagagggat
     1621 tcagacagca gcgtcctgac tagatccggg gattgcatta gacacagcag tacttttgac
     1681 cagactgcgg agcgggacag cggcctgggc gccaccggag ggtcggtggt ggtagtagtg
     1741 gcctccatgc agggggcgca cgggcccgag tcgggaactg acagctcctt gactgctgtg
     1801 tcacagctac ccccgtcgga gaaaatgagc cagcccactc cggcccagcc gcagagtttt
     1861 agcgttgggc agccacagcc gccgccgcca cccgtaggtg gggctgtggc tcaaagctcg
     1921 gctccgctgc cgccgttccc gggagccgcg accgggccgc agccaatgat ggcagccgcg
     1981 cagcccagcc agccccaggg agcggggccc gggggacaga ctctgccgcc gacgaatgta
     2041 accctggcgc agccggctat gtccctgcct ccgcagccgg gccctgcagt gggcgccccc
     2101 gcggcgcagc agccccagca gttcgcgtat cctcagcctc agataccgcc cggacatttg
     2161 ctgcccgtcc agccctccgg ccagagtgag tacctgcagc agcacgtggc cggcctgcag
     2221 ccgccaagcc ccgcgcagcc ctcgtccacc ggcgccgcag cgagccccgc cacggcggcc
     2281 acccttcccg tgggcaccgg ccagaatgct tcctcggtgg gcgcgcagct catgggcgcg
     2341 tcttcccagc ccagcgaagc catggccccc cggacgggac cagcgcaagg cgggcaggtc
     2401 gcgccttgtc agccgactgg agtgcccccg gctactgtgg gaggcgtggt gcagccgtgc
     2461 ctcggtcctg ccggggctgg gcagccccag tccgtgcctc cgccgcagat gggtggcagt
     2521 ggtccgctgt cagccgtacc tggtggccct cacgccgtgg tgcccggagt tccaaacgtg
     2581 cctgcagccg tgcccgctcc aagcgtgcct agtgtgtcta ccacttctgt tactatgcca
     2641 aatgtacccg cgcctctggc ccagtcgcaa cagctgagca gccatacgcc agtcagcagg
     2701 agcagcagca taatccagca tgttgggctg cccttagcgc caggcacaca cagcgcacca
     2761 acaagtctac cacagtctga cctaagccag tttcaaactc agacccagcc tttagtcggg
     2821 caagtcgacg atactagaag aaaatcagaa cccctacctc aaccaccact ttctctcatt
     2881 gctgaaaata agcctgttgt gaagccgcct gttgcagatt ccctggcaaa cccccttcag
     2941 ttaacaccta tgaacagtct ggccacctct gtattcagca tagctattcc tgttgatggt
     3001 gatgaagaca gtgcatctgg gggaggtgtt gtagccattg acaacaaaat agaacaagca
     3061 atggatctgg tgaaaagcca tttgatgtat gcagtaagag aagaagtgga agttttaaag
     3121 gaacaaataa aagaattagt tgaaagaaac tctttacttg aacgagaaaa tgcactgtta
     3181 aaatctcttt caagcaatga tcaattatcc caactcccaa cccaacaggc caatcctggt
     3241 agcacttctc aacagcaagc agtgatagca cagcctccgc agccaacgca acctccacag
     3301 cagccgaatg tctcctcagc ataaagcttt cttaagcctc attaagaaaa aaactgaaag
     3361 caatctatcc ttgtgtgcca ctggtgttct ttccacttta tacgaaagca agtagccatg
     3421 ctttggttgt gtgtttggcc ttttcagtat tagacaatca ttctacaaga gcttttcctc
     3481 tctctgagat gtcatgcagc gctgttgatg tccagttcta tgtcatcagt acacaaggag
     3541 aataatagat ggggtttatt aaagcgagca aagtctgcat tttacctggt gcgcatgagt
     3601 ggggtcttta agagttttgg tggctctccc atgtttccta ttacccatgg atttaccctg
     3661 agccttccta tcacattata aataacagtt catctaaaga gccacttttc tttctgattc
     3721 agtaacattt gcctacataa gttttcattt atttgtgttt tatttattac agggctgcta
     3781 ttttcataat gtacatgaac aatgtcacag aactttttta atttttttga ataattataa
     3841 gtatcagtaa aggaagtgaa agacaggatt gcatttaata gataaaacgt ttaggcaata
     3901 attgaacaaa agaatcctgg catatttcta acactaatgg caatttactt atggtattta
     3961 ttttcagtag taaagaccca gcttgaatgt aaattttgta tagtgtaagt atgaagaaca
     4021 tagtgcaact gtacaggtag tcaccagtta ttgtgatatg ataaataatt gggctatttt
     4081 gatgaagaaa actttgttca tttgtttcta ctttctaaga gaaattgcca cgattcctct
     4141 gcttttcaac atttcgtatg actttttttt cgggtgggaa taaaaagctg tgaaattgtt
     4201 caacctactt tgtaaccaaa gaagcaaagc tgtgtaatgg agtttggttt ttttttgttg
     4261 tttttttttt tttgtctttt ttttttttta taatgcacat tctttatgta tttttattta
     4321 gtgttttctc agtcacaatt ttctttactg tctagcatga tctgcatgac ctataatctt
     4381 tgaaccactt tcgtacctca tgtttttatc cagcactctt attgtaatat gtactagtct
     4441 gtgaacaatg tcaaataaaa gagaacgaac aggtagtttg gtggagctga gctagtgtac
     4501 aatacactag ttgtaaaaaa aaaaaaaaaa aaaagtgagc catcttttgt tcatttaaaa
     4561 tggtgttttg aatttcgtat gcagaaaacg ttttgttaca ttgcagattt taatgtattt
     4621 aataaatgca acatgcagat taagtgcagt gtatactgag tatttaaatt aaaatgtaca
     4681 tttcataaat acagtttcaa aagaaagcat cattttgtgt atactaacac attaagtgta
     4741 tgtcagaaat tgatgtataa atatatattt taacattttc tgaaattaaa ctgcagtttt
     4801 gttgaaatat ttggctctat atgtgttcaa aatttgacta gatttgtatt ttcacagttc
     4861 aaaatactcc ttaaagacct gttaataatg acttactgat agttcttaaa ttttcaaagt
     4921 aaaacaattt aaagaatgca aaaatagtga gcaatatttg tactcaatat tcatattatg
     4981 ttagtgatac ttaagtagga aatccttgtc taccaaaact tttacttgaa ttcagaaagg
     5041 tgcctgtaga aattaacatg cactgcatct gtagccagct agctaatcag agttaatgag
     5101 aaccagtagt tagttctccg tgttaaaaga cttatttgaa gtaaaaattt aatcagatta
     5161 gccacaaaca aacaaggcat aaaattggga tgtacatgag attgccaaat attttctatt
     5221 ctagttgtgc tgcaagacat tgaacaaaat gttaatgaat aacattataa atcaaggaac
     5281 taagtacata ggcttgagta agcaacccta aggatgtgta gtaagattcc aagtagagct
     5341 gctacaggtg agatacttaa tggaatgtga aacttcactg ttggactgtt aaccgaaaag
     5401 gtttgctggc tgtgcccctc cagtgctaac tgtgacatgt gatatttaat acaggtggag
     5461 aaaaactagt cttagaaatg ggatttttgt gaaaatagat tccattttga ttctggggct
     5521 ggaaaaacac ttcataaaca aagtaatagt ctccaaggaa atgaataata aggtttaaaa
     5581 tggtaacagc agttattact acttttatat taagaaaaac agtagaaaag gagagaaaat
     5641 atgggtagtt cacagaagag ataaatggca gtttctaaag gttcaacctc tagtaaccta
     5701 agaaatgcaa attaatgttt ttggcgaatt gtaataagtg tcctaagtag caggacttaa
     5761 catagaaaga aagacaggct ttcctgtgta cttttagaaa gtaatttggc agtatgtatc
     5821 tagagcctta aataataagc tttccagtgt aacgacagga gaagatactc aactcatggt
     5881 ccattgttca aaaatgcaga attattgtgg tgtctgccct ctgctttccc atcatcccct
     5941 cccctcaaaa tccaaactat ccctactgtc aacccttccc ccagctgatt gtttaggatc
     6001 ttaagtaact tccaattcag ctttggagat ttagtctcct gctccaaata gttgtaaata
     6061 ttaactgatt gagatcccaa tttggaggca cagtcttaca ggaatgccat ctatctaatt
     6121 ctgtcagtgt aatgcccata gagcaaggtc ccacataagc ttttctgttt taatttagcc
     6181 ggtaaatcat aagtagaaat ttttctgata cggtaattgt ctacaattca aacctaactt
     6241 tccccaaata aaatttagtt tgttccgatg tgatctcaca gcttgccaca ccaaaaacaa
     6301 atagtagggg ttaggaggca ttaaataaac agtttcctta actcctgatt gtccaaaata
     6361 gctgaatttt tccaattcta tttataatat cctatgctaa ttagaagtat tgagataacg
     6421 ttttataatc aagcctccag tgactgggga tcacagctgc cattattcac aagaattttc
     6481 acaggtgaat gattatattt ctccaagttg caaactccag taattcatgg accttttttt
     6541 aaaaaaaaaa aaagtcttaa tagaaccaga atatctctgt tcttgaaaat ttatctttgc
     6601 agaaccagaa atagctaatt gtctggaaac aatttttaag atttgaagtc acatttttaa
     6661 aagaagcttt atggctcagt ggcaagcact ttgtgacatt tctttaataa tcctatgaaa
     6721 aggacaaaac ccagccctca aaattgacaa tttgaggaca taataagcca actgataatg
     6781 cagtgaggaa tacttgttac tatgtacagt cagggatggt tttgtcaggg taggtttcct
     6841 caaaaacatg acacctgagt cttgaaggtc ataagagcca gagggaaggg tgttccaagc
     6901 acaagaaaag agaactcaat ggcttaaaag tataaacaag gctcctaggg cagccacaga
     6961 attgcattgg agacaggcag gcagtgactt ccccactgac ctagtatttt ggcatctatt
     7021 cagaaagcaa gaatgattta ttgaatggtt ttaagcaatg ggatactatg ctcaaattat
     7081 gctggatgtg cacaattcca gtggcagatg ggaggtaggt tagagtagct gaaactggaa
     7141 gctgagaaat aatttaggag ggatgacaat ccggaaaata aatgaaaggg caaaattaca
     7201 taggcaattt tgtgaaggtc atgaaggaag tttgtcttgg gtctttctgt accattttcc
     7261 tctccacaat gaacatccca ttcccagtgt caggtatcag gtgcctattt tctttttttc
     7321 ttttttcttt tttttttttt ttgagacaga gtcttgctgt gctcaggctg gagtgcagtg
     7381 gcaagatctc ggctcactgc aacctccgcc tcccaggttc aagcgattct cctgcctcag
     7441 cctcctgagt agctgggatt acaggcgtgt gccaccatgc caggctaatt tttgtatttt
     7501 tagtagagac agggtttcac catgttggcc aggttggtct tgaactcctg acctcaagtc
     7561 atctcccccg cccgcctggg cctcccaaag tgttgggatt acaggcgtga gccaccgcac
     7621 ccagctgcct attttcttgt ccctccaaaa aaagattaaa gagtcccaac tcatacttga
     7681 aagtttgact cggtcttttc cttccaacag ttataaatct gtgaaatgaa agtctttgtt
     7741 tccttaaagg gatttcaaga atttaacaaa agggttgggg aatggaacct ttgctgtcac
     7801 tatgcctgtc tctcaattct accctaaagg taaactaaat ggaatccttt tatggtgact
     7861 aggattagga tgttgattcc aaagcctttc cctaaaatct attccttgct ccctggaaag
     7921 gataatacag gttctggccc ccactgtagg tactgacagc ccaaggcaga ataggttttg
     7981 ctaggcttta gccttttatt ctttgacata ggtcagtcct gcaggcttct tttttctaag
     8041 tctcctgcct atccaaaacc agaccttttc tcccacctcc ttctgcttca agcctcttaa
     8101 taatccatat ttctattttc tagcaatagt aatagtttat tatttagtga tgtatacttg
     8161 caccccattt actccttttc aatatttcag gatacatctt tgtcctgtat ttctgtacca
     8221 gttcacagaa tttctacagc aagatccctt taaatgtatg gctttccttc ataggttgca
     8281 aggattactt tatacaagtg ctactgtaac tatatctttt gtggagtaca attggtatga
     8341 catgctgtgt tcatagatga aaatgctaag tgaggcactc tgaagcaagt taaagttcac
     8401 agacattctt gcttgcccac cacccccccg aaccccgcca tggaatcatc aaataagaaa
     8461 aataagacag attttcagat tggtgatggc aaagtaagac gctggtattt tgcactggcg
     8521 cagtccattt agttgttgaa tttgttcagc atacctggat ggtgagtgag ttagctttat
     8581 ttttcattct cagcagggaa actgatagac taggcagttt ggggaatttt cccattgtct
     8641 actgtcatgg gtccatagta tttaagttta cagactacaa cattgtccac agaagccata
     8701 tttatcccct tggagggaag ggaaaggtgt gctaacttag atcataaaaa caactgaatt
     8761 ttaaaacaag gcaaatgcca cagttcaact tctccataaa aaagttaagt tcatctctcc
     8821 attcttttgc atgcctacaa tatttccata taacaactgt aaaatactct tgcctgctgc
     8881 aatccaaatg ttatccggat cacagttctt gctgtcattt cacaggcatg caatgtatct
     8941 atagataggt catatcatac ctgaggaaga tcagagacag caagggaaca ttttccccac
     9001 acttgagatg gaaataataa caggtcagtg gctaataaaa agaaacatgt atgactttgt
     9061 aaaaccctaa aagtgatgtt ttcaagaaat ttaggtttct tttttttatt ttataaaaaa
     9121 aatgttgaca tgcaatttaa ttctatttca atttaggtgc tgcaacctgt actggatgtt
     9181 tggggataga aagatgaatg ggacatgatt tgtgtgctcc aatgagctca gtccagtgta
     9241 agtgtaaaag ttaaagtgcc atattgttta ttcagagtat atgttatagt ttgtgaacct
     9301 tatatcattt gttaaatgaa aggtttttat aaatcaacag taatattgcc atcccatgta
     9361 aaaatccttt gatattgcca ggaaacaact ttaaatcacc agaaagcttt aacatcaaat
     9421 aatactttta gaaggagggg aaaagccttc cacctcgaca tcgagcctat gaagtattcc
     9481 ctaaaaaaga ggaactaatc tgagaaaagt ttacacattt ttaatactct aagttgtaga
     9541 taatgaaatt gatttaaatc aaagttaact agaataggat tcatgtgtca tgaattatat
     9601 tgtctattgt ctcataagca acaagtgaca cttattatct tggctgacaa aatcagctac
     9661 catttaatga accaagttaa gaataactca ggtgactgat accaaaaata aatctctaat
     9721 acagctgaga atccagaaac acaaataata tgttgagtaa gtaataaaag tgacatttca
     9781 aattagtaag gaaaaaatgg attattcaac aaatagtatg ggatagcata acaatttaga
     9841 agaaatataa agttagatct ttcacaaaaa ttaattctag ctagactagc agttagtctt
     9901 ctactttttg gttactccct tttagaaatt ggaagctatg aagctatata ccctgtccat
     9961 ttgaaaatgc acataagcaa agaactttac aaataggtta taaagagaag ctcaatctca
    10021 gcaaaataag ggaagtgcac attaaaacac aagaaaccat ctcagatcca actaatattt
    10081 agcgtgcata caaatcaaaa gctggaaagg gtgtggaaaa acaggaatac attgctagtg
    10141 agcatacaaa ttggcacagc ctcttttaaa gacaattgtg tatcacccat taaattttaa
    10201 aagtcacata ccctgtgacc taacaattcc aacttttaat gtcctaaaga aacatacatg
    10261 tgttcacaag atagcctgta caatgatgtc aattgcacct ttgcttgtac agtagacccc
    10321 cttatctgca ttttcacttt ttgaggtttc agtcatccaa ggtcaaccac aatacaaaaa
    10381 tattaaatgg aaaattccag aaacgaacat aagttttaaa cttcaaacct ttctgcatag
    10441 tataataaaa atctctcact atccctctcc atcctgcctg ggacaggaat catccctttg
    10501 tccagtgtat ccacaatgta tacactcatt atatacccat tttataatgc ccattagtca
    10561 tttctattat cagatcataa aaaaacatag tacatagagg gtttggtatt attcatggtt
    10621 tcaggcattc agtgagggtc ttggaaaata tcctccatga tgggggagct actgtaatac
    10681 tatgaaattg gaaaaaccaa aatactcatc aagaggatgg actatggtac acatgatgaa
    10741 gtattaagta tcaatgaaaa agagagagtc ctacatgtcc ggcatggaag gatctccagg
    10801 agacaatgtt aaaagtttgg gggaaaaatt gaaagacaat acctgtaata catatgatac
    10861 tagtgatgtt aaatcacatc acacacacac acacacacac acacacacac acacaaatac
    10921 tgtatagtgt acttctacaa gtacatattt gtgtattata tagagagaag gtctggaagg
    10981 atactaacca aactgtcaac agtagtttgg tagcgtgcat tagctttaac tgtaatgttt
    11041 gatgtttttg aatatgcatt tatgtattgt ttgtgtaatg acaaattaat aaattagaaa
    11101 taaaataaaa
//
